Spider silk, renowned for its high performance and biodegradable nature, holds significant promise across various industries seeking alternatives to unsustainable petroleum-based fibres. However, despite advancements in the artificial spinning of spider silk fibres, much remains to be uncovered to achieve both high yield and the exceptional mechanical properties characteristic of native silks.To address this challenge, the study delved into the expression patterns across 291 spider individuals and their silk compositions, aiming to elucidate the factors influencing the toughness, tensile strength, strain at break, and young's modulus of silk fibres. Through comprehensive analysis, the study sheds light on the challenges of conducting quantitative analyses across diverse species. Despite limitations arising from evolutionary distant samples and homologous clustering, the novel analysis method revealed signals of combinatory protein expressions and their impact on the mechanical properties of the silk fibre. The quantitative expression levels appear to contain valuable information when considered alongside protein sequences and motifs within Spidroin proteins, indicating paths for further analysis.The methodology shows promise, and the findings suggest clear directions for future research, paving the way for optimizing silk production and harnessing its full potential in diverse applications.