Bacteria rarely live alone. Instead, they exist in complex communities where interactions between neighbouring cells affect their survival, adaptation, and ability to thrive in a shared environment. These interactions range from cooperative behaviours that help bacteria survive together to antagonistic strategies that suppress competing microorganisms. Understanding how bacteria balance cooperation and competition is important for explaining microbial community behaviour, especially in biofilms associated with chronic and recurrent infections.
Biofilms are a major challenge in healthcare because they are difficult to treat and often resist antibiotics. They are involved in many diseases, including catheter-associated urinary tract infections (CAUTI), ventilator-associated pneumonia (VAP), chronic wound infections, and infections associated with medical devices. These infections often involve multiple bacterial species coexisting at the same site. However, it is still not fully understood what determines whether bacteria compete, coexist, or support each other's survival.
This thesis studies how bacterial communities interact in different environments, focusing on how nutrient conditions, metabolic adaptation, and antagonistic behaviour shape bacterial survival. Using clinical isolates, laboratorystrains, biofilm models, metabolomics, and screening methods, this work examines interactions ranging from cooperationvia shared metabolism (Papers I-III) to direct chemical competition via growth inhibition (Paper IV).
Paper I studied the growth of Staphylococcus aureus and Pseudomonas aeruginosa in mono- and dual-cultures using clinical isolates from patients with VAP. Prior growth of S. aureus did not help P. aeruginosa subsequently attach to a surface. However, growing the two species together altered the bacterial growth, biofilm formation, the balance between species, and their metabolism. Higher biofilm biomass did not necessarily mean both species benefited equally; viable cell counts indicated that P. aeruginosa grew more than S. aureus in dual-species biofilms in the presence of glucose. These effects clearly depended on nutrient conditions and differed between clinical isolates and laboratory reference strains, showing that each patient's microenvironment can shape how bacteria survive.
Paper II studied interactions between Escherichia coli and P. aeruginosa under conditions similar to those in a urinary catheter. In an artificial urine medium, P. aeruginosa was the dominant species in dual-species biofilms, while nutrient-rich conditions allowed E. coli to compete more effectively or even become dominant in co-culture. Metabolomics analyses showed that both species adapted differently under varying growth conditions, and that their co-culture metabolic profile differed from those of either monoculture. This suggests that nutrient availability, together with how each species adjusts its metabolism, determines which species becomes dominant in catheter-associated biofilms.
Paper III, building on these findings, used a criss-cross sequential cultivation method to test whether the two species exchange metabolites directly. It was observed that E. coli consistently released succinate, which was later taken up by P. aeruginosa, showing a clear producer–consumer relationship. Other metabolites, including citrate, itaconate, cadaverine,and phenylalanine, showed patterns suggesting additional, more complex forms of metabolic exchange or species-specificmetabolism. These results suggested that nutrient sharing between species helps explain how these two clinically relevant bacteria coexist in nutrient-poor, host-associated environments, such as those in CAUTI. Unlike these cooperative interactions, bacterial communities can also be shaped by competition.
Paper IV studied the ability of marine bacteria to produce antimicrobial compounds as an example of this competitive strategy. One Bacillus subtilis isolate excreted compounds with antibacterial activity against other bacterial species, indicating that competition is an important survival strategy and that this isolate could be a potential source of new antibacterial compounds.
Overall, this thesis demonstrated that bacteria could interact in many different ways, from sharing nutrients and adapting together to actively suppressing one another. Which strategy occurs depends on the bacterial species involved and the environment they live in, showing that bacteria adjust their behaviour rather than relying on a single fixed way of coexisting. These findings offer new insight into how microbial communities function and may help improve the treatment of biofilm-related infections and also the discovery of new antibacterial compounds.