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  • 901.
    V. Piletska, Elena
    et al.
    Cranfield University, Bedford MK45 4DT, England.
    R. Guerreiro, Antonio
    Cranfield University, Bedford MK45 4DT, England.
    Romero-Guerra, Maria
    Cranfield University, Bedford MK45 4DT, England.
    Chianella, Iva
    Cranfield University, Bedford MK45 4DT, England.
    P. F. Turner, Anthony
    Cranfield University, UK.
    A. Piletsky, Sergey
    Cranfield University, Bedford MK45 4DT, England.
    Design of molecular imprinted polymers compatible with aqueous environment2008Inngår i: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 607, nr 1, s. 54-60Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The main problem of poor water compatibility of molecularly imprinted polymers (MIPs) was addressed in examples describing design of synthetic receptors with high affinity for drugs of abuse. An extensive potentiometric titration of 10 popular functional monomers and corresponding imprinted and Blank polymers was conducted in order to evaluate the subtleties of functional groups ionisation under aqueous conditions. It was found that polymers prepared using 2-trifluoromethacrylic acid (TFMAA) in combination with toluene as porogen possess superior properties which make them suitable for effective template recognition in water. The potential impact of phase separation during polymerisation on formation of high quality imprints has been discussed. Three drugs of abuse such as cocaine, deoxyephedrine and methadone were used as template models in polymer preparation for the practical validation of obtained results. The polymer testing showed that synthesized molecularly imprinted polymers have high affinity and selectivity for corresponding templates in aqueous environment, with imprinting factors of 2.6 for cocaine and 1.4 for methadone and deoxyephedrine. Corresponding Blank polymers were unable to differentiate between analytes, suggesting that imprinting phenomenon was responsible for the recognition properties. (c) 2007 Elsevier B.V. All rights reserved.

  • 902.
    Vagin, Mikhail Y
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Eriksson, Mats
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Boron-doped diamond microelectrode arrays for electrochemical monitoring of antibiotics contamination in water2014Inngår i: 15th International Conference on Electroanalysis (ESEAC), 2014Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    The improvement of water management and increasing the access to safe drinking water can develop the quality of life for millions of people world-wide and reduce child mortality due to water-borne diseases [1]. Sweden was recently affected by the lack of appropriate water management which resulted in microbial contamination and tens of thousands of people falling ill [2]. Pollution with chemical compounds is also a waterworks concern. The appearance of pharmaceuticals such as antibiotics in raw water affects the cleaning processes at waterworks [3]. Substances which are not, or are only partly, eliminated in the sewage treatment plant will reach the surface water where they may affect organisms of different trophic levels and cause, for example, the of antibiotics resistance [4]. The inhibition of bacteria of waste water plants by antibiotics may seriously affect organic matter degradation. The efficiency of nitrification as an important step in waste water purification, can be decreased by antibiotics inhibition [5]. Boron-doped diamond (BDD) is an advanced electrode material that possesses the combination of good electrical conductivity achieved via film doping and the extreme chemical inertness of diamond, which gives rise to a number of highly desirable properties of BDD as electrode material: a wide potential window in aqueous media allows electrochemical measurements at both extreme anodic and cathodic potentials, very low capacitive currents leads to a sensitivity increase and extreme chemical and structural inertness prevents electrode fouling [6]. Usage of a microelectrode array as the working electrode offers a variety of benefits for electroanalysis: an improvement of the analytical performance in comparison with macroelectrodes under planar diffusion, higher signal-to-noise ratios due to low capacitive currents at the small surface area, shorter response times and less sensitivity to variations in the water flow rate. The BDD arrays of this work contain 2900 microelectrodes (10 mm diameter each) and have been used for the detection of antibiotics (ofloxacine and canamycin A) in water with high amplitude pulse voltammetry processed by multivariate data analysis. The detection limits observed in monitoring mode were comparable with the characteristics of standard protocols of antibiotics detection, which opens the possibility for continuous monitoring of water.

    [1] The United Nations, World Water Development Report 4, 2012; [2] Lindberg, A. et al.,

    FOI-R--3376--SE, 2011; Dryselius, R.; National Food Agency, Sweden, 2012; [3] Kummerer

    K. Chemosphere, 2009, 75, 417; [4] Kummerer K. Chemosphere, 2009, 75, 435; [5]

    Dokianakis, S.N. et al., Water Sci. Technol. 50, 341; [6] Goeting, C. et al.,

    NewDiam.Front.C.Tech. 1999, 9, 207; Compton, R. et al., Electroanal. 2003, 15, 1349.

     

  • 903.
    Vagin, Mikhail Y
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Sekretaryova, Alina N
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Reategui, Rafael Sanchez
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Eriksson, Mats
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Screen-printed graphite microbands as a versatile biosensor platform2014Inngår i: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    The use of extremely small working electrodes offers a variety of benefits for electroanalysis. The enhanced mass transport as a result of convergent diffusion is the most important advantage of microdimensional electrodes and results in improved of analytical performance The low detectable-currents problem can be solved by single microelectrode multiplication into an array, thus combining the advantages of enhanced mass transport and high output signals. The microband is one of the most cost-effective and easy-fabricated geometries for microelectrodes. The microband width is a critical microscopic dimension of the electrode, which maintains the dominance of convergent diffusion, whereas the microband length is macroscopic and ensures registration of high currents.

    Graphite screen-printing on a plastic support is a standard technology for large-scale production of low cost electrochemical devices. This has been combined with simple guillotine cutting to fabricate of microband arrays for autonomous environmental and clinical monitoring.

    Single-layer and multilayer microband arrays of different band lengths were produced and characterised using optical and electrochemical methods. The critical dimension for the microband width to facilitate convergent diffusion was assessed electrochemically and found to be in the order of 5 microns. The developed electrode structures were used as a versatile platform for the manufacture of model electroanalytical systems. Direct oxidation of ascorbic acid was explored at the microband arrays and a glucose biosensor based on mediated and immobilised glucose oxidase was fabricated. Both examples yielded significant enhancement of the analytical performance.

    A: the layout of the screen-printed graphite microband array of 5 electrode layers. B: voltammmetric responses obtained at the microband arrays.

    Acknowledgement: Formas and Security Link for financial support; David Nilsson (Acreo) for screen-printing.

  • 904.
    Vagin, Mikhail Y
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Sekretaryova, Alina N
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Reategui, Rafael Sanchez
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Eriksson, Mats
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Screen-printed graphite microbands for electroanalysis2014Inngår i: 15th International Conference on Electroanalysis (ESEAC), 2014Konferansepaper (Annet vitenskapelig)
  • 905.
    Vagin, Mikhail Yu.
    et al.
    Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem.
    Jeerapan, Itthipon
    Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Hat Yai, Songkla, Thailand.
    Wannapob, Rodtichoti
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hat Yai, Songkla, Thailand.
    Thavarungkul, Panote
    Hat Yai, Songkla, Thailand.
    Kanatharana, Proespichaya
    Hat Yai, Songkla, Thailand.
    Anwar, Nargis
    Dublin Road, Dundalk, County Louth, Ireland.
    McCormac, Timothy
    Dublin Road, Dundalk, County Louth, Ireland.
    Eriksson, Mats
    Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem.
    Turner, Anthony P.F
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Jager, Edwin W.H.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensor- och aktuatorsystem. Linköpings universitet, Tekniska fakulteten.
    Wing Cheung, Mak
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensor- och aktuatorsystem. Linköpings universitet, Tekniska fakulteten.
    Water-processable polypyrrole microparticle modules for direct fabrication of hierarchical structured electrochemical interfaces2016Inngår i: Electrochimica Acta, ISSN 0013-4686, E-ISSN 1873-3859, Vol. 190, s. 495-503Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Hierarchically structured materials (HSMs) are becoming increasingly important in catalysis, separation and energy applications due to their advantageous diffusion and flux properties. Here, we introduce a facile modular approach to fabricate HSMs with tailored functional conducting polypyrrole microparticles (PPyMP). The PPyMPs were fabricated with a calcium carbonate (CaCO3) template-assisted polymerization technique in aqueous media at room temperature, thus providing a new green chemistry for producing water-processable functional polymers. The sacrificial CaCO3 template guided the polymerization process to yield homogenous PPyMPs with a narrow size distribution. The porous nature of the CaCO3 further allows the incorporation of various organic and inorganic dopants such as an electrocatalyst and redox mediator for the fabrication of functional PPyMPs. Dawson-type polyoxometalate (POM) and methylene blue (MB) were chosen as the model electrocatalyst and electron mediator dopant, respectively. Hierarchically structured electrochemical interfaces were created simply by self-assembly of the functional PPyMPs. We demonstrate the versatility of this technique by creating two different hierarchical structured electrochemical interfaces: POM-PPyMPs for hydrogen peroxide electrocatalysis and MB-PPyMPs for mediated bioelectrocatalysis. We envision that the presented design concept could be extended to different conducting polymers doped with other functional organic and inorganic dopants to develop advanced electrochemical interfaces and to create high surface area electrodes for energy storage.

  • 906.
    Wang, Shenqi
    et al.
    Cranfield University.
    Ye, F
    NTU.
    Lang, X
    NTU.
    Fei, D
    De Monfort University.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Ge, Yi
    Cranfield University.
    Detection of [Ca2+]i Changes in Sub-plasma Membrane Microdomains in a Single Living Cell by an Optical Fiber-based Nanobiosensor2014Inngår i: Austin Journal of Nanomedicine and Nanotechnology, ISSN 2381-8956, Vol. 2, nr 4, s. 1022-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    n optical fiber-based nanobiosensor, for advanced detection of [Ca2+]i (i.e. intracellular Ca2+ concentration) changes in sub–plasma membrane microdomains in a single living smooth muscle cell and a single living cardiomyocyte, was successfully prepared by coating silver and then immobilizing Calcium Green–1 Dextran, a calcium ion sensitive dye, on the distal end of the nanoprobe. The constructed nanobiosensor was capable of detecting ultra–low and local intracellular calcium ion concentration within the nanomolar range, which is around the physiological level of free cytosolic calcium ion in a single living cell. The response time was less than milliseconds enabling the detection of transient elementary calcium ion signaling events associated with calcium ion microdomains. The effects of stimulants such as high potassium buffer solution and norepinephrine solution were also investigated. The resulting system could thus greatly facilitate the development of an advanced nano–diagnostic platform for in vivo and real–time sensing/diagnosing of [Ca2+]i at the single cell level.

  • 907.
    Wannapob, Rodhichoti
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Vagin, Mikhail
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Tekniska fakulteten.
    Liu, Yu
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Mak, Wing Cheung
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Functional Microparticles – “LEGO” for Printable Bioelectronics2017Inngår i: 26th Anniversary World Congress on Biosensors (Biosensors), Elsevier, 2017, Vol. 27, s. 3-3Konferansepaper (Annet vitenskapelig)
  • 908.
    Wannapob, Rodtichoti
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Prince Songkla University, Thailand.
    Vagin, Mikhail
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Liu, Yu
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Sichuan Agriculture University, Peoples R China.
    Thavarungkul, Panote
    Prince Songkla University, Thailand.
    Kanatharana, Proespichaya
    Prince Songkla University, Thailand.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensor- och aktuatorsystem. Linköpings universitet, Tekniska fakulteten.
    Mak, Wing Cheung
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Printable Heterostructured Bioelectronic Interfaces with Enhanced Electrode Reaction Kinetics by Intermicroparticle Network2017Inngår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 9, nr 38, s. 33368-33376Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Printable organic bioelectronics provide a fast and cost-effective approach for the fabrication of novel biodevices, while the general challenge is to achieve optimized reaction kinetics at multiphase boundaries between biomolecules and electrodes. Here, we present an entirely new concept based on a modular approach for the construction of heterostructured bioelectronic interfaces by using tailored functional "biological microparticles" combined with "transducer micro particles" as modular building blocks. This approach offers high versatility for the design and fabrication of bioelectrodes with a variety of forms of interparticle spatial organization, from layered structures to more advance bulk heterostructured architectures. The heterostructured biocatalytic electrodes delivered twice the reaction rate and a six-fold increase in the effective diffusion kinetics in response to a catalytic model using glucose as the substrate, together with the advantage of shortened diffusion paths for reactants between multiple interparticle junctions and large active particle surface. The consequent benefits of this improved performance combined with the simple means of mass production are of major significance for the emerging printed electronics industry.

  • 909.
    White, SF
    et al.
    ; .
    Tothill, IE
    ; .
    Newman, JD
    ; .
    Turner, APF
    Cranfield University, UK.
    Development of a mass-producible glucose biosensor and flow-injection analysis system suitable for on-line monitoring during fermentations1996Inngår i: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 321, nr 03-feb, s. 165-172Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Screen printed amperometric glucose biosensors were constructed and optimised for use in a flow injection analysis (FIA) system. The sensors were fabricated using a catalytic metallised carbon based ink, allowing a working potential of +350 mV (Ag/AgCl). Overall the sensors displayed a high degree of reliability and robustness. A linear response was obtained over the range 0.1 to 25 mM glucose with a slope of 1.35 +/- 0.20 mu A/mM. The within electrode coefficient of variation at each concentration tested (n = 10) was less than 2% (or 0.1 mM for concentrations below 5 mM). In addition, it was demonstrated that a single sensor can be used in the FIA system over a seven day period indicating a high level of operational stability. These sensors fulfil the criteria necessary for a mass produced device, which would be suitable for the on-line monitoring of glucose consumption during microbial fermentation.

  • 910.
    WHITE, SF
    et al.
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    TURNER, APF
    Cranfield University, UK.
    BILITEWSKI, U
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    SCHMID, RD
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    BRADLEY, J
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,BEDFORD MK43 0AL,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    LACTATE, GLUTAMATE AND GLUTAMINE BIOSENSORS BASED ON RHODINISED CARBON ELECTRODES1994Inngår i: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 295, nr 3, s. 243-251Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Amperometric enzyme sensors for lactate, glutamate and glutamine were constructed using rhodinised carbon electrodes. All three sensors operated at a potential of +400 mV (Ag/AgCl). Lactate sensors were constructed using lactate oxidase immobilized in hydroxyethylcellulose, overlaid with a cellulose acetate membrane. Using a flow-injection system, the sensors had a linear range of 0.1 to 1.5 mM. Measurements of lactate concentrations from a mammalian cell culture were compared with results obtained from a commercial instrument. A correlation coefficient of r=0.982 (n = 15) was obtained. Glutamate and glutamine sensors were fabricated based on glutaraldehyde immobilization; the former by incorporating glutamate oxidase and the latter based on glutamate oxidase and glutaminase (both had a linear range of 0.1 to 1.5 mM). The feasibility of using these sensors, in conjunction with a previously described glucose sensor, for mammalian cell culture monitoring is discussed

  • 911.
    WHITE, SF
    et al.
    GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
    TURNER, APF
    Cranfield University, UK.
    BILTEWSKI, U
    GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
    BRADLEY, J
    GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
    SCHMID, RD
    GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,W-3300 BRAUNSCHWEIG,GERMANY; .
    ONLINE MONITORING OF GLUCOSE, GLUTAMATE AND GLUTAMINE DURING MAMMALIAN-CELL CULTIVATIONS1995Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 10, nr 07-jun, s. 543-551Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Amperometric biosensors (based on rhodinised carbon electrodes) for glucose, glutamine and glutamate were constructed. The sensors were incorporated into a three cell parallel FIA system and used to monitor the three analytes on-line during two mammalian cell perfusion cultures. All measurements were made simultaneously from undiluted media sample. Use of the FIA system enabled easy and rapid exchange of the sensors, during cultivation. The inclusion of a calibration step, regularly for all sensors, helped to maintain the accuracy of all measurements. Comparison with off-line measurements indicated that all three biosensors operated successfully, providing accurate information.

  • 912.
    WHITE, SF
    et al.
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    TURNER, APF
    Cranfield University, UK.
    SCHMID, RD
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    BILITEWSKI, U
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    BRADLEY, J
    CRANFIELD UNIV,CRANFIELD BIOTECHNOL CTR,CRANFIELD MK43 0AL,BEDS,ENGLAND; GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY; .
    INVESTIGATIONS OF PLATINIZED AND RHODINIZED CARBON ELECTRODES FOR USE IN GLUCOSE SENSORS1994Inngår i: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 6, nr 8, s. 625-632Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Graphite electrodes were modified by the electrodeposition of either platinum or rhodium, using cyclic voltammetry. Following only brief periods nf treatment, plantinized carbon electrodes were able to directly oxidize glucose at a potential of +350 mV (vs. Ag/AgCl). By narrowing both the potential range of deposition (-450 to -500 mV) and cycling period (50 seconds at a scan rate of 10 mV s(-1)), the effects of direct glucose oxidation could be significantly: reduced. Detection of H2O2 at +400 mV was possible; glucose enzyme electrodes were constructed, with glucose oxidase immobilized in hydroxyethylcellulose and operated at this potential. These sensors had a linear range of 0.1 to 25 mM glucose and a slope of 8 nA/mM. Other electrodes were prepared by the electrodeposition of rhodium. These base electrodes proved to be insensitive to glucose and produced higher currents (compared to low platinum electrodes) to H2O2. Glucose sensors were constructed using rhodinized electrodes. Again operating at +400 mV, these sensors had a linear range from 0.1 to 20 mM and a slope of 21 nA/mM glucose.

  • 913.
    Wilson, PK
    et al.
    Cranfield University, UK.
    Jiang, T
    Hunan Normal University, PR China.
    Minunni, ME
    Università delgi Studi di Firenze, Italy.
    Turner, A P F
    Cranfield University, UK.
    Mascini, M
    Università delgi Studi di Firenze, Italy.
    A novel optical biosensor format for the detection of clinically relevant TP53 mutations2005Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 20, nr 11, s. 2310-2313Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The TP53 gene has been the subject of intense research since the realisation that inactivation of this gene is common to most cancer types. Numerous publications have linked TP53 mutations in general or at specific locations to patient prognosis and therapy response. The findings of many studies using general approaches such as immunohistochemistry or sequencing are contradictory. However, the detection of specific mutations, especially those occurring in the structurally important L2 and L3 zinc binding domains, which are the most common sites of TP53 mutations, have been linked to patient prognosis and more strongly to radiotherapy and chemotherapy resistance in several major cancers. In this study, the TI-SPR-1 surface plasmon resonance system and Texas Instruments Spreeta (TM) chips were used to develop a DNA biosensor based on thiolated probes complementary to these domains. The sensors were able to detect these mutations in both oligonucleotides and PCR products with normal and mutant TP53 DNA, but the difference in hybridisation signal was small. Preliminary experiments to enhance the signal using Escherichia coli mismatch repair proteins, MutS and single strand binding protein were carried out. It was found that MutS was unable to bind to mismatch oligonucleotides, but single strand binding protein was able to bind to single stranded probes, which had not hybridised to the target, resulting in a three-fold increase in the sensitivity of the biosensor. While further work needs to be carried out to optimise the system, these preliminary experiments indicate that the TI-SPR-1 can be used for the detection of clinically relevant mutations in the TP53 gene and that the sensitivity can be increased significantly using single strand binding protein. This system has a number of advantages over current mutation detection technologies, including lower cost, ease of sensor preparation and measurement procedures, technical simplicity and increased speed due to the lack of need for gel electrophoresis.

  • 914.
    WILSON, R
    et al.
    CRANFIELD INST TECHNOL,CTR BIOTECHNOL,CRANFIELD MK43 0AL,BEDS,ENGLAND; QUEEN ELIZABETH MED CTR,WOLFSON RES LABS,BIRMINGHAM B15 2TH,W MIDLANDS,ENGLAND; .
    TURNER, APF
    Cranfield University, UK.
    GLUCOSE-OXIDASE - AN IDEAL ENZYME1992Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 7, nr 3, s. 165-185Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    The properties of glucose oxidase (GOD) are described in relation to the widespread use of this enzyme in biosensors, The shortcomings of other enzymes that oxidize glucose are indicated. Isolation of and early work on the enzyme are briefly reviewed. The sources and the physical characteristics of the enzyme are listed. The structure of GOD and how it relates to the immobilization and stability of the enzyme is described along with other factors that influence stability. The role of flavin adenine dinucleotide and attempts to use it in immobilization of the enzyme are described. Inhibitors of the enzyme are listed, and substrates of the enzyme and the reaction mechanism are considered in detail. The role of electron acceptors in amperometric biosensors is described, and alternatives to their use are highlighted. Other uses of the enzyme are described and the properties of the enzyme are summarized with reference to a commercially successful biosensor.

  • 915.
    Yarman, Aysu
    et al.
    Fraunhofer Institute for Biomedical Engineering, Germany and Potsdam University, Germany.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Scheller, Frieder
    Fraunhofer Institute for Biomedical Engineering, Germany and Potsdam University, Germany.
    Electropolymers for(nano-) imprinted biomimetic sensors2014Inngår i: Nanosensors for chemical and biological applications: sensing with nanotubes, nanowires and nanoparticles / [ed] Kevin C Honeychurch, Woodhead Publishing Limited, 2014, s. 125-149Kapittel i bok, del av antologi (Annet vitenskapelig)
    Abstract [en]

    Part one reviews the range electrochemical nanosensors, including the use of carbon nanotubes, glucose nanosensors, chemiresistor sensors using metal oxides and nanoparticles. Part two discusses spectrographic nanosensors such as surface-enhanced Raman scattering (SERS) nanoparticle sensors

  • 916.
    Yarman, Aysu
    et al.
    Fraunhofer Institute for Biomedical Engineering IBMT, Potsdam, Germany.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Scheller, Frieder
    Fraunhofer Institute for Biomedical Engineering IBMT, Potsdam, Germany.
    Synergy between Enzymes and MIPs2013Inngår i: Nano-scaled arrangements of proteins, aptamers, and other nucleic acid structures – and their potential applications, COST Thematic Workshop, Leipzig: Helmholtz Zentrum für Umweltforschung , 2013Konferansepaper (Annet vitenskapelig)
  • 917.
    Zhang, Juankun
    et al.
    Tianjin University Science and Technology, Coll Biotechnol, Minist Educ, Key Lab Ind Microbiol, Tianjin, Peoples R China.
    Dong, Shanmu
    Tianjin University Science and Technology.
    Lu, Jinhui
    Tianjin University Science and Technology.
    Turner, Anthony P. F.
    Cranfield University, UK.
    Fan, Qingjie
    Lanlike Elect Chemistry High Tech Ltd, Tianjin, Peoples R China.
    Jia, Shiru
    Tianjin University Science and Technology.
    Yang, Hongjiang
    Tianjin University Science and Technology.
    Qiao, Changsheng
    Tianjin University Science and Technology.
    Zhou, Hao
    Tianjin University Science and Technology.
    He, Guowei
    TEDA Int Cardiovasc Hospital, Tianjin, Peoples R China.
    A Label Free Electrochemical Nanobiosensor Study2009Inngår i: Analytical Letters, ISSN 0003-2719, E-ISSN 1532-236X, Vol. 42, nr 17, s. 2905-2913Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Nano-porous silicon (PS) is an attractive material for incorporation into biosensors, because it has a large surface area combined with the ability to generate both optical and electrical signals. In this paper, we describe a label-free nanobiosensor for bovine serum albumin (BSA). Nano-porous silicon produced in our laboratory was functionalized prior to immobilization of anti-BSA antibody on the surface. Reaction with BSA in phosphate buffered saline (PBS) buffer resulted in an impedance change which was inversely proportional to the concentration of the analyte. The system PBS buffer/antigen-antibody/PS constitutes an electrolyte-insulator-semiconductor (EIS) structure, thus furnishing an impedance EIS nanobiosensor. The linear range of the sensor was 0-0.27mgmL-1 and the sensitivity was less than 10 mu g mL-1.

  • 918.
    Zhang, Juankun
    et al.
    Tianjin University of Science and Technology, Peoples R China .
    Wu, Yan
    Tianjin University of Science and Technology, Peoples R China .
    Zhang, Binbin
    Tianjin University of Science and Technology, Peoples R China .
    Li, Min
    Tianjin University of Science and Technology, Peoples R China .
    Jia, Shiru
    Tianjin University of Science and Technology, Peoples R China .
    Jiang, Shuhai
    Tianjin University of Science and Technology, Peoples R China .
    Zhou, Hao
    Tianjin University of Science and Technology, Peoples R China .
    Zhang, Yi
    Tianjin University of Science and Technology, Peoples R China .
    Zhang, Chaozheng
    Tianjin University of Science and Technology, Peoples R China .
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    LABEL-FREE ELECTROCHEMICAL DETECTION OF TETRACYCLINE BY AN APTAMER NANO-BIOSENSOR2012Inngår i: Analytical Letters, ISSN 0003-2719, E-ISSN 1532-236X, Vol. 45, nr 9, s. 986-992Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A novel aptamer nano-porous silicon (PS) biosensor was investigated for the rapid determination of tetracyclines. Electrochemical impedance spectroscopy (EIS) was used to analyze the behavior of the sensor. The specific binding of tetracycline to the aptamer biosensor led to a decrease in impedance. The corresponding impedance spectra (Nyquist plots) were obtained when serial concentrations of tetracycline were added into the system. An equivalent electrical circuit was used to fit the impedance data. The linear range of the sensor was 2.1-62.4 nM.

  • 919.
    Zhang, Juankun
    et al.
    Tianjin University Science and Technology, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China; Cranfield University, Silsoe, Beds, England; Lanlike Tianjin Electron Chemistry High Tech Ltd, Tianjin, Peoples R China; .
    Zhang, Weiliang
    Tianjin University Science and Technology, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China; Cranfield University, Silsoe, Beds, England; Lanlike Tianjin Electron Chemistry High Tech Ltd, Tianjin, Peoples R China; .
    Dong, Shanmu
    Tianjin University Science and Technology, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China; Cranfield University, Silsoe, Beds, England; Lanlike Tianjin Electron Chemistry High Tech Ltd, Tianjin, Peoples R China; .
    P. F. Turner, Anthony
    Cranfield University, UK.
    Fan, Qingjie
    Tianjin University Science and Technology, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China; Cranfield University, Silsoe, Beds, England; Lanlike Tianjin Electron Chemistry High Tech Ltd, Tianjin, Peoples R China; .
    Jia, Shiru
    Tianjin University Science and Technology, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China; Cranfield University, Silsoe, Beds, England; Lanlike Tianjin Electron Chemistry High Tech Ltd, Tianjin, Peoples R China; .
    Nano-porous light-emitting silicon chip as a potential biosensor platform2007Inngår i: Analytical Letters, ISSN 0003-2719, E-ISSN 1532-236X, Vol. 40, nr 8, s. 1549-1555Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Nano-porous silicon (PS) offers a potential platform for biosensors with benefits both in terms of light emission and the large functional surface area. A light emitting PS chip with a stable and functional surface was fabricated in our laboratory. When protein was deposited on it, the light emission was reduced in proportion to the protein concentration. Based on this property, we developed a rudimentary demonstration of a label-free sensor to detect bovine serum albumin (BSA). A serial concentration of BSA was applied to the light chip and the reduction in light emission was measured. The reduction of the light intensity was linearly related to the concentration of the BSA at concentrations below 10(-5) M. The detection limit was 8 x 10(-9) M.

  • 920.
    Zhybak, Mikael
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Dempsey, Eithne
    Centre for Research in Electroanalytical Technologies, Department of Science, Dublin, Ireland.
    Vagin, Mikhail Y
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Korpan, Yaroslav
    Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
    Copper/Nafion/PANI Nanocomposite as an electrochemical transducer for creatinine and urea enzymatic biosensing2014Inngår i: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    Chronic Kidney diseases (CKD) affect, to different degrees, ca. 25 million Americans and 19 million Europeans. Monitoring of creatinine and urea levels is of great importance for a correct evaluation of the status of patients and for their treatment. In this paper, we present the development of creatinine and urea enzymatic biosensors, based on a novel ammonium ion-specific Copper/Nafion/Polyanyline (PANI) nanocomposite electrode (Fig. 1A), and suitable for PoC and decentralised diagnostic applications. . Studies on the nanocomposite electrode revealed its high sensitivity and specificity towards ammonium, in respect to amino acids, creatinine and urea, with response range between 5 and 75 μM (Fig. 1B) and with a detection limit of 1 μM. To demonstrate its suitability as transducer in biosensors, creatinine and urea biosensors were fabricated by immobilising creatinine deiminase or urease, respectively, on the nanocomposite surface. Optimisation of the enzyme immobilisation demonstrated that the incorporation of lactitol markedly improved the stability of the biosensors. The response range of the creatinine biosensor was 2 to 100 μM, which fits well with the normal levels of creatinine in healthy people (30-150 µM).

    The urea biosensor had a response range of 5 to 100 µM. A limit of quantification of 1 µM was achieved for both the biosensors.

    Evaluation of the performance of the biosensors in real sample matrices and cross reactivity studies are currently on-going. We envisage that the proposed design will be particularly compatible with fully-printed systems thus offering a viable route to the mass production of inexpensive sensors for mobile health.

     

     

  • 921.
    Zhybak, Mikael T
    et al.
    Institute of Molecular Biology and Genetics, NAS of Ukraine, Ukraine.
    Fayura, L.Y.
    Institute of Cell Biology, NAS of Ukraine, Ukraine.
    Boretsky, Yu R
    Institute of Cell Biology, NAS of Ukraine, Ukraine.
    Dempsey, Eithne
    Centre for Research in Electroanalytical Technologies, Ireland.
    Gonchar, M.V.
    Institute of Cell Biology, NAS of Ukraine, Ukraine.
    Sibirny, A.A.
    Institute of Cell Biology, NAS of Ukraine, Ukraine.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Korpan, Yaroslav
    Institute of Molecular Biology and Genetics, NAS of Ukraine, Ukraine.
    Novel L-arginine amperometric assay based on recombinant arginine deiminase and Nafion/PANi composite2016Inngår i: Biosensors 2016 – The World Congress on Biosensors, Elsevier, 2016Konferansepaper (Annet vitenskapelig)
  • 922.
    Zhybak, M.T.
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Vagin, Mikhail
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska fakulteten.
    Dempsey, Eithne
    Centre for Research in Electroanalytical Technologies, Department of Science, ITT Dublin, Tallaght, Dublin, Ireland.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Korpan, Y
    Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine,Kyiv, Ukraine.
    Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 77, s. 505-511Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The use of a novel ammonium ion-specific copper-polyaniline nano-composite as transducer for hydrolase-based biosensors is proposed. In this work, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle. Immobilisation of enzymes was shown to be a crucial step in the development of the biosensors; the use of glycerol and lactitol as stabilisers resulted in a significant improvement, especially in the case of the creatinine, of the operational stability of the biosensors (from few hours to at least 3 days). The developed biosensors exhibited high selectivity towards creatinine and urea. The sensitivity was found to be 85±3.4 mA M−1 cm−2 for the creatinine biosensor and 112±3.36 mA M−1 cm−2 for the urea biosensor, with apparent Michaelis–Menten constants (KM,app), obtained from the creatinine and urea calibration curves, of 0.163 mM for creatinine deaminase and 0.139 mM for urease, respectively. The biosensors responded linearly over the concentration range 1–125 µM, with a limit of detection of 0.5 µM and a response time of 15 s.

    The performance of the biosensors in a real sample matrix, serum, was evaluated and a good correlation with standard spectrophotometric clinical laboratory techniques was found.

  • 923.
    Zhybak, Mykhailo T.
    et al.
    National Academic Science Ukraine, Ukraine.
    Fayura, Lyubov Y.
    NAS Ukraine, Ukraine.
    Boretsky, Yuriy R.
    Lviv State University of Phys Culture, Ukraine.
    Gonchar, Mykhailo V.
    NAS Ukraine, Ukraine.
    Sibirny, Andriy A.
    NAS Ukraine, Ukraine; Rzeszow University, Poland.
    Dempsey, Eithne
    ITT Dublin, Ireland.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Korpan, Yaroslav I.
    National Academic Science Ukraine, Ukraine.
    Amperometric L-arginine biosensor based on a novel recombinant arginine deiminase2017Inngår i: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 184, nr 8, s. 2679-2686Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The authors describe an amperometric biosensor for the amino acid L-arginine (L-Arg). It is based on the use of a Nafion/Polyaniline (PANi) composite on a platinum screen-printed electrode (Pt-SPE) using a novel recombinant arginine deiminase isolated from Mycoplasma hominis. The protein was over-expressed, purified and employed as a biorecognition element of the sensor. Enzymatic hydrolysis of L-Arg leads to the formation of ammonium ions which diffuse into the Nafion/PANi layer and induce the electroreduction of PANi at a potential of -0.35 V (vs Ag/AgCl). L-Arg sensitivity is 684 +/- 32 A.M-1.m(-2), and the apparent Michaelis-Menten constant K-M(app)) is 0.31 +/- 0.05 mM. The calibration plot is linear over the range 3-200 mu M L-Arg, the limit of detection is 1 mu M, and the response time (for 90% of the total signal change to occur) is 15 s. The sensor is selective and exhibits good storage stability (amp;gt; 1 month without loss in signal). The biosensor was applied to the analysis of L-Arg in pharmaceutical samples and of ammonium and L-Arg in spiked human plasma obtained from blood of healthy volunteers and those with a hepatic disorder. Data generated were found to be in good agreement with a reference fluorometric enzymatic assay.

  • 924.
    Zhybak, Mykhailo T.
    et al.
    Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi. Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, 03680, Ukraine .
    Vagin, Mikhail Yu.
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten.
    Beni, Valerio
    ACREO Swedish ICT, -601 74, Norrköping, SE, Sweden .
    Liu, Xianjie
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska fakulteten.
    Dempsey, Eithne
    Centre for Research in Electroanalytical Technologies, Department of Science, Institute of Technology Tallaght, Tallaght, Dublin, Ireland .
    Turner, Anthony P. F.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Korpan, Yaroslav I.
    Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, 03680, Ukraine .
    Direct detection of ammonium ion by means of oxygen electrocatalysis at a copper-polyaniline composite on a screen-printed electrode.2016Inngår i: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 183, nr 6, s. 1981-1987Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A novel electrocatalytic material for oxygen reduction, based on polyaniline in combinationwith copper, was developed and utilised for the direct voltammetric quantification of ammonium ions. Consecutive electrode modification by electrodeposited copper, a Nafion membrane and electropolymerised polyaniline resulted in an electrocatalytic composite material which the retained conductivity at neutral pH. Ammonia complex formation with Cu (I) caused the appearance of oxygen electrocatalysis, which was observed as an increase in cathodic current. This Faradaic phenomenon offered the advantage of direct voltammetric detection and was utilised for ammonium electroanalysis. The developed quantification protocol was applied for ammonium assay in human serum and compared with the routine approach for clinical analysis.

  • 925. Zourob, Mohammed
    et al.
    Elwary, SounaTurner, AnthonyCranfield University, UK.
    Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems.2008Collection/Antologi (Fagfellevurdert)
    Abstract [en]

    Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems will cover the up-to-date biosensor technologies used for the detection of bacteria. Written by the world's most renowned and learned scientists each in their own area of expertise, Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems is the first title to cover this expanding research field

  • 926.
    Özgür, Erdogan
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hacettepe University, Turkey.
    Parlak, Onur
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten. RISE Acreo, Sweden.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Uzun, Lokman
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hacettepe University, Turkey.
    Bioinspired design of a polymer-based biohybrid sensor interface2017Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 251, s. 674-682Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The key step in the construction of efficient and selective analytical separations or sensors is the design of the recognition interface. Biomimicry of the recognition features typically found in biological molecules, using amino acids, peptides and nucleic acids, provides plausible opportunities to integrate biological molecules or their active sites into a synthetic polymeric backbone. Given the basic role of functional amino acids in biorecognition, we focused on the synthesis of polymerizable amino acid derivatives and their incorporation into a polymer-based biohybrid interface to construct generic bioinspired analytical tools. We also utilized polyvinyl alcohol (PVA) as a sacrificial polymer to adjust the porosity of these biohybrid interfaces. The surface morphologies of the interfaces on gold electrodes were characterized by using scanning electron (SEM) and atomic force (AFM) microscopies. The electrochemical behavior of the polymeric films was systematically investigated using differential pulse voltammetry (DPV) to demonstrate the high affinity of the biohybrid interfaces for Cu(II) ions. The presence of macropores also significantly improved the recognition performance of the interfaces while enhancing interactions between the target [Cu(II) ions] and the functional groups. As a final step, we showed the applicability of the proposed analytical platform to create a Cu(II) ion-mediated supramolecular self-assembly on a quartz crystal microbalance (QCM) electrode surface in real time. (C) 2017 Elsevier B. V. All rights reserved.

  • 927.
    Özgür, Erdogan
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hacettepe University, Department of Chemistry, Ankara, Turkey.
    Parlak, Onur
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Uzun, Lokman
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hacettepe University, Department of Chemistry, Ankara, Turkey.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Porous functional nanofilms for designing bioinspired sensor surfaces2015Inngår i: Proceeding of Advanced Materials World Congress / [ed] Ashutosh Tiwari, Linköping, Sweden: VBRI Press , 2015Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    Bio-mimicking of recognition features typical of biological molecules such as aminoacids, peptides, nucleic acid etc. is leading to the design and development of novel functional (artificial) materials for chemical/biochemical sensing applications. The insertion of biological molecules or their active sites into the backbone of synthetic polymers is one of the possible ways to achieve this. Herein, we synthesised a polymerisable derivative of an amino acid (L-histidine) through a set of reactions with 1-(1Hbenzo[d][1,2,3]triazol-1-yl)-2-methylprop-2-en-1-one (MA-Bt), since amino acid residues are the origin for the functional properties and highly selective substrate-binding ability of many extended biological structures. We obtained a functional monomer methacryloylamidohistidine (MAH), which was polymerised with 2-hydroxyethyl methacrylate in presence of polyvinyl alcohol (PVA) on a gold surface. The aim of using PVA was to obtain highly porous polymeric structure. For control purpose, polymers without MAH and PVA were also synthesised. The morphology of the polymeric film on gold surface was characterized with scanning electron microscopy (SEM) and atomic force microscopy (AFM). The obtained polymer showed significant affinity for Cu2+. The electrochemical behaviour of the polymeric films was systematically investigated with differential pulse voltammetry (DPV). The presence of pores was shown to significantly improve the recognition performance of the film.

16171819 901 - 927 of 927
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