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  • 301.
    Nguyen, Thi Thanh Van
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Wibberg, Daniel
    Vigil-Stenman, Theoden
    Battenberg, Kai
    Demchenko, Kirill
    Blom, Jochen
    Fernandez, Maria
    Yamanaka, Takashi
    Berry, Alison
    Kalinowski, Jörn
    Brachmann, Andreas
    Pawlowski, Katharina
    Metagenomes from the earliest divergent Frankia cluster: They come in teamsManuskript (preprint) (Annet vitenskapelig)
  • 302. Nicolis, Stamatios C.
    et al.
    Detrain, Claire
    Demolin, Didier
    Deneubourg, Jean-Louis
    Optimality of collective choices: A stochastic approach2003Inngår i: Bulletin of Mathematical Biology, Vol. 65, nr 5, s. 795-808Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Amplifying communication is a characteristic of group-living animals. This study is concerned with food recruitment by chemical means, known to be associated with foraging in most ant colonies but also with defence or nest moving. A stochastic approach of collective choices made by ants faced with different sources is developed to account for the fluctuations inherent to the recruitment process. It has been established that ants are able to optimize their foraging by selecting the most rewarding source. Our results not only confirm that selection is the result of a trail modulation according to food quality but also show the existence of an optimal quantity of laid pheromone for which the selection of a source is at the maximum, whatever the difference between the two sources might be. In terms of colony size, large colonies more easily focus their activity on one source. Moreover, the selection of the rich source is more efficient if many individuals lay small quantities of pheromone, instead of a small group of individuals laying a higher trail amount. These properties due to the stochasticity of the recruitment process can be extended to other social phenomena in which competition between different sources of information occurs. (C) 2003 Society for Mathematical Biology. Published by Elsevier Ltd. All rights reserved.

  • 303.
    Nilsson, Caroline
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Myskoxe - bevarande i Sverige2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Myskoxen dog ut i Sverige för 40 000 år sedan men har levt i Kanada fram tills idag. 1947introducerades myskoxar till Dovrefjell i Norge och år 1971 vandrade 5 individer över tillHärjedalen i Sverige. Populationen har som mest varit uppe i 30 individer på 1980-talet menhjorden består nu av endast 8 stycken individer. Med hjälp av bidrag från privatpersoner ochEU har man kunnat arbeta med att bevara myskoxarna men populationen står inför vissaproblem. Den svenska myskoxpopulationen anses vara i riskzonen för inavel, dock vet maninte detta med säkerhet då det är otydligt hur nära besläktade individerna i ursprungshjordenfrån 1971 var. Överlag har myskoxarna i hela världen en låg genetisk variation men man harsett att det i små populationer, som den i Sverige, går att återfå en viss variation genomavelsprogram med individer från andra populationer, som Grönland. Ett annat problem för enlite population som den svenska är demografisk stokasticitet, hur ålder- och könsfördelningenser ut. Då den svenska hjorden till exempel bara innefattar en könsmogen hane skulle detpåverka populationen mycket negativt om han dog eller blev infertil. Myskoxar i Kanada,Grönland och Norge har utsatts för diverse parasiter och virus som ofta har en dödlig utgång,men den svenska populationen har lyckats undgå de flesta av dem. Det kan dock finnas ensmittorisk vid inplantering av nya myskoxar från andra populationer eller om den norska ochden svenska populationen kommer i kontakt med varandra. Ett annat problem som densvenska myskoxen står inför är acceptans av befolkningen som bor i området där den rör sig.Det har dokumenterats endast tre olyckor där människor blivit skadade i samband medmyskoxar på 40 år men vissa komplikationer har uppkommit i samband med rennäring iområdet. För att ha en frisk och stark myskoxepopulation i Sverige behöver man först ochfrämst introducera nytt genetisk material genom avel med myskoxar från exempelvisGrönland eller Kanada. Man bör samtidigt se vad man kan göra för till exempel rennäringensom skulle påverkas mer om hjorden blev större. Den låga genetiska variationen ochminskningen av denna är det största hotet mot den svenska populationen och bör hanteras såsnart som möjligt för att säkra de svenska myskoxarnas överlevnad.

  • 304.
    Nilsson, Frida
    Karlstads universitet.
    Vad händer med maten vi äter?: Barns tankar om matsmältningen och kroppens inre organ2014Independent thesis Advanced level (professional degree), 20 poäng / 30 hpOppgave
    Abstract [sv]

    Barn har olika uppfattningar och tankar om kroppens inre vilket jag anser är intressant. Syftet med denna undersökning var att ta reda på vad elever i årskurs ett har för uppfattning kring matsmältningsprocessen. Vad som händer med maten och vilka organ i kroppen som bearbetar maten. Jag ville också ta reda på om de hade någon uppfattning om matsmältningsorganens placering och funktion i vår kropp.

    Elevernas uppfattning varierade då vissa elever hade stora kunskaper om vad som hände med maten medan vissa hade mindre uppfattningar men som utvecklades under intervjun. Alla elever visste att maten hamnade i magsäcken men därefter hade en del av eleverna svårigheter med vad som senare skulle hända med maten mer än att den kommer ut som avföring.

    Elevernas tidigare erfarenheter om kroppen gjorde att deras uppfattningar om vad som händer med maten i vår kropp varierade. 

  • 305.
    Nilsson, Jessica
    Högskolan i Skövde, Institutionen för vård och natur.
    Dynglevande skalbaggar i Västra Götalands län: En jämförelse av dyngbaggefaunan på två olika habitat2010Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    De dynglevande skalbaggarna är en stor tillgång för naturen och för människan. Dyngbaggarna lever både i skogen och på öppna marker och bryter ner spillning från såväl vilda som tama djur. En stor del av de dynglevande skalbaggarna tillhör gruppen bladhorningar (Scarabaeidae) och hela 29 arter av totalt 61 är med på den svenska rödlistan. För att ta reda på hur dyngbaggefaunan ser ut på en ekologisk gård i Tämta, Västra Götalands län, gjordes inventeringar vid tre tidpunkter under sommaren 2009. Varje inventeringstidpunkt bestod av två delinventeringar, en på öppen betesmark och en på betesmark i skog, detta för att se om det fanns några skillnader i artantal och om det var samma arter på de olika habitaten. De båda habitaten betades under perioden av nötkreatur. Samtliga skalbaggar samlades in för att sedan artbestämmas på labb och sammanlagt påträffades 13 olika arter varav en av dem, streckdyngbaggen (Aphodius merdarius), finns med på rödlistan som starkt hotad.

    Av de 13 arter som hittades återfanns 11 av dem på den öppna betesmarken jämfört med skogsmarkens 9 arter. Antalet individer varierade vid de olika tidpunkterna och även om skogsbetesmarken hade fler arter under augustiinventering var det fler individer vid motsvarande inventering på den öppna betesmarken, vilket tyder på en större täthet på de öppna markerna. Flest individer var det på den öppna betesmarken under juli månad. Slutsatsen jag drar av resultaten, med stöd från tidigare studier, är att lokalernas utformning och läge har stor betydelse för dyngbaggarna, men kanske än viktigare för deras levnad och överlevnad är tillgången på bra substrat för boplats och föda och hur platsen i fråga är solexponerad.

  • 306.
    Nissling, Anders
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Limnologi.
    Thorsen, Anders
    da Silva, Filipa F.G
    Fecundity regulation in relation to habitat utilisation of two sympatric flounder (Platichtys flesus) populations in the brackish water Baltic Sea2015Inngår i: Journal of Sea Research, ISSN 1385-1101, E-ISSN 1873-1414, Vol. 95, s. 188-195Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Two populations of flounder (Platichtys flesus) with different life history traits inhabit the brackish water Baltic Sea. Both types share feeding areas in coastal waters during summer-autumn but utilise different habitats for spawning in spring, namely offshore spawning with pelagic eggs and coastal spawning with demersal eggs respectively. Fecundity regulation by atresia was assessed as prevalence (portion of fish with atresia) and intensity (calculated as the average intensity of atresia in these fish) during the reproductive cycle following start of gonad development in the autumn up to spawning in spring, and evaluated in relation to fish condition (Fulton's condition factor reflecting energy reserves of the fish) and feeding incidence of the respective population. Peaking in winter (December–February), fecundity regulation was significantly higher for coastal spawning flounder than for flounder spawning offshore. For coastal spawners, the prevalence was 45–90% with an intensity of 6.4–9.3% vs. 0–25% and an intensity of 2.1–3.4% for offshore spawners during winter. Further, fecundity regulation ceased prior to spawning for offshore spawners but continued for coastal spawners. For coastal spawners, the prevalence was 12–29% and an intensity of 2.5–6.1% during spawning. The change in fish condition was strongly related to feeding incidence and differed between populations. As feeding ceased, condition of offshore spawners decreased during winter up to spawning, whereas condition of coastal spawners decreased during autumn but was maintained as feeding started again prior to spawning. Thus, habitat utilisation according to spawning strategy affects the timing of fecundity down-regulation reflecting availability of resources, namely limited food resources in deep areas and higher availability in coastal areas. Offshore spawning flounder display characteristics typical for a capital spawner with ceasing of feeding and oocyte down-regulation well before spawning, whereas coastal spawning flounder can be characterised as intermediate between a capital and income spawner with feeding prior to and during spawning along with continuous fecundity-regulation.

  • 307.
    Nordén, Britta
    Högskolan i Gävle, Akademin för teknik och miljö, Avdelningen för elektronik, matematik och naturvetenskap.
    Beskrivning av förökningstekniker för nio perenner och en klätterväxt i zon 52014Independent thesis Basic level (university diploma), 10 poäng / 15 hpOppgave
  • 308.
    Näsström, Åsa
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska högskolan.
    The relationship between personality and basal metabolic rate in Red Junglefowl (Gallus gallus)2013Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    ‘Animal personality’ refers to individual behavioural differences that are consistent over time and context. Physiological constraints are suggested to underlie this constraint in behavioural plasticity. As energy is required for physiological processes that generate behaviour, energy metabolism could be a proximate explanation for personality. Currently, the most coherent framework linking behaviour, metabolism and life history-traits is still poorly tested empirically, and studies are showing contradicting results. Therefore, I here aim to explore this relationship further by investigating the relationship between basal metabolic rate (BMR) and personality in Red Junglefowl (Gallus gallus). Birds used had known responses in personality assays, and their metabolic rates were measured by determining oxygen consumption in standardized conditions throughout the night using an open respirometry system. BMR was negatively correlated with time spent foraging, and positively correlated with time spent being vigilant. Considering foraging an ‘activity’ (due to its energy-demand), my results support the allocation model, a model that assumes that an animal has a fixed amount of energy, thus that an energetic trade-off occur between competing energy requiring processes such as BMR and activity. Hence, an animal with low BMR has more energy to spend on activity. However, I do not consider vigilance as an energy-demanding activity; hence this relationship cannot be interpreted in this framework. Taken together, my results show a relationship between personality and BMR, although their relationship still needs further investigation to understand the causality and consequences of it.  

  • 309. Olenina, I
    et al.
    Hajdu, S
    Edler, L
    Andersson, A
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap.
    Wasmund, N
    Busch, S
    Göbel, J
    Gromisz, S
    Huseby, Siv
    Huttunen, M
    Jaanus, A
    Kokkonen, P
    Ledaine, I
    Niemkiewicz, E
    Biovolumes and size-classes of phytoplankton in the Baltic Sea2006Inngår i: HELCOM, Balt. Sea Environ. Proc, Vol. 106, s. 1-144Artikkel i tidsskrift (Fagfellevurdert)
  • 310.
    Olsson, Daniel
    et al.
    Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för miljö- och livsvetenskaper (from 2013).
    Gericke, Niklas
    Karlstads universitet, Fakulteten för samhälls- och livsvetenskaper, Avdelningen för biologi. Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för miljö- och livsvetenskaper (from 2013).
    Boeve-de Pauw, Jelle
    Research Unit Edubron, Department of Training and Education Sciences, Faculty of Social Sciences, University of Antwerp, Belgium.
    Berglund, Teresa
    Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för miljö- och livsvetenskaper (from 2013).
    Chang, Tzuchau
    Graduate Institute of Environmental Education, National Taiwan Normal University, Taiwan.
    Manuscript Green schools in Taiwan: Effects on student sustainability consciousnessManuskript (preprint) (Annet vitenskapelig)
  • 311.
    Olsson, Emma
    Karlstads universitet, Fakulteten för samhälls- och livsvetenskaper.
    Hur pedagoger i förskolan tillämpar tiden i skogsmiljö: utifrån gynnande och bevarande av hållbar utveckling inom biologisk mångfald2013Independent thesis Advanced level (professional degree), 10 poäng / 15 hpOppgave
  • 312.
    Onuţ-Brännström, Ioana
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    Tibell, Leif
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    Johannesson, Hanna
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    A worldwide phylogeography of the whiteworm lichens Thamnolia reveals three lineages with distinct habitats and evolutionary histories2017Inngår i: Ecology and Evolution, ISSN 2045-7758, E-ISSN 2045-7758, Vol. 7, nr 10, s. 3602-3615Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Thamnolia is a lichenized fungus with an extremely wide distribution, being encountered in arctic and alpine environments in most continents. In this study, we used molecular markers to investigate the population structure of the fungal symbiont and the associated photosynthetic partner of Thamnolia. By analyzing molecular, morphological, and chemical variation among 253 specimens covering the species distribution range, we revealed the existence of three mycobiont lineages. One lineage (Lineage A) is confined to the tundra region of Siberia and the Aleutian Islands, a second (Lineage B) is found in the high alpine region of the Alps and the Carpathians Mountains, and a third (Lineage C) has a worldwide distribution and covers both the aforementioned ecosystems. Molecular dating analysis indicated that the split of the three lineages is older than the last glacial maximum, but the distribution ranges and the population genetic analyses suggest an influence of last glacial period on the present-day population structure of each lineage. We found a very low diversity of Lineage B, but a higher and similar one in Lineages A and C. Demographic analyses suggested that Lineage C has its origin in the Northern Hemisphere, possibly Scandinavia, and that it has passed through a bottleneck followed by a recent population expansion. While all three lineages reproduce clonally, recombination tests suggest rare or past recombination in both Lineages A and C. Moreover, our data showed that Lineage C has a comparatively low photobiont specificity, being found associated with four widespread Trebouxia lineages (three of them also shared with other lichens), while Lineages A and B exclusively harbor T. simplex s. lat. Finally, we did not find support for the recognition of taxa in Thamnolia based on either morphological or chemical characters.

  • 313. Orchard, S.
    et al.
    Heck, A.
    Uhlén, Mathias
    KTH, Skolan för bioteknologi (BIO), Proteomik (stängd 20130101).
    Ping, P.
    Publication Committee Meeting HUPO 5th Annual World Congress Long Beach, CA, USA 30 October 20062007Inngår i: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 7, nr 7, s. 1009-1011Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This meeting brought together delegates from industry, academia and the publishing houses to facilitate discussions on the level of support from the journals for the use of standardised data formats and their interest in the creation of a network of proteomics repositories collaborating on a coordinated data curation effort. Discussions centred on how best to structure interactions between journals, databases and researchers to improve accessibility to data, and facilitate comparisons between datasets.

  • 314.
    Orro Graña, Adolfo
    SciLifeLab Stockholm.
    Examination of the role of binding site water molecules in molecular recognition2012Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
    Abstract [en]

    A set of algorithms were designed, implemented and evaluated in order to, first, identifyclusters of conserved waters in binding pockets, i.e. hydration sites. Then, their contributionto the free energy of binding in a ligand-protein association was quantified by calculatingtheir enthalpy and entropy. The information obtained by using these algorithms couldcontribute to the development of new drugs by generating new ligands that target specifichigh-energy, unfavorable waters. Evaluation tests show that our algorithms can indeedprovide relevant data about how hydration sites influence ligand-protein binding.

  • 315.
    Palmqvist, K
    et al.
    Umeå University, Umeå, Sweden.
    Campbell, D
    Mount Allison University, Sackville, Canada.
    Ekblad, Alf
    Swedish University of Agricultural Sciences, Umeå, Sweden.
    Johansson, H
    Umeå University, Umeå, Sweden.
    Photosynthetic capacity in relation to nitrogen content and its partitioning in lichens with different photobionts1998Inngår i: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 21, nr 4, s. 361-372Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We tested the hypothesis that lichen species with a photosynthetic CO2-concentrating mechanism (CCM) use nitrogen more efficiently in photosynthesis than species without this mechanism. Total ribulose bisphosphate carboxylase-oxygenase (Rubisco; EC 4.1.1.39) and chitin (the nitrogenous component of fungal cell walls), were quantified and related to photosynthetic capacity in eight lichens. The species represented three modes of CO2 acquisition and two modes of nitrogen acquisition, and included one cyanobacterial (Nostoc) lichen with a CCM and N2 fixation, four green algal (Trebouxia) lichens with a CCM but without N2 fixation and three lichens with green algal primary photobionts (Coccomyxa or Dictyochloropsis) lacking a CCM. The latter have N2-fixing Nostoc in cephalodia. When related to thallus dry weight, total thallus nitrogen varied 20-fold, chitin 40-fold, Chl a 5-fold and Rubisco 4-fold among the species. Total nitrogen was lowest in three of the four Trebouxia lichens and highest in the bipartite cyanobacterial lichen. Lichens with the lowest nitrogen invested a larger proportion of this into photosynthetic components, while the species with high nitrogen made relatively more chitin. As a result, the potential photosynthetic nitrogen use efficiency was negatively correlated to total thallus nitrogen for this range of species. The cyanobacterial lichen had a higher photosynthetic capacity in relation to both Chl a and Rubisco compared with the green algal lichens. For the range of green algal lichens both Chl a and Rubisco contents were linearly related to photosynthetic capacity, so the data did not support the hypothesis of an enhanced photosynthetic nitrogen use efficiency in green-algal lichens with a CCM.

  • 316.
    Palmé, Anna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Wennerström, Lovisa
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Guban, Peter
    Laikre, Linda
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Stopping compensatory releases of salmon in the Baltic Sea. Good or bad for Baltic salmon gene pools?: Report from the Baltic Salmon 2012 symposium and workshop, Stockholm university February 9-102012Rapport (Annet vitenskapelig)
  • 317.
    Palmé, Anna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Wennerström, Lovisa
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Guban, Peter
    Ryman, Nils
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Laikre, Linda
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Compromising Baltic salmon genetic diversity: Conservation genetic risks associated with compensatory releases of salmon in the Baltic Sea2012Rapport (Annet vitenskapelig)
  • 318.
    Palmé, Anna
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Wennerström, Lovisa
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Guban, Peter
    Ryman, Nils
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Laikre, Linda
    Stockholms universitet, Naturvetenskapliga fakulteten, Zoologiska institutionen, Avdelningen för populationsgenetik.
    Conclusions on conservation genetic risks associated with compensatory releases of salmon in the Baltic Sea.: A brief summary of a synthesis report to the Swedish Agency for Marine and Water Management.2012Rapport (Annet vitenskapelig)
  • 319.
    Pedro J, Aphalo
    et al.
    University of Helsinki, Helsinki, Finland.
    Albert, Andreas
    Helmholtz Zentrum, München, Germany.
    McLeod, Andy
    University of Edinburgh, Edinburgh, UK.
    Heikkilä, Anu
    Finnish Meteorological Institute, Helsinki, Finland.
    Gómez, Iván
    López Figueroa, Felix
    University Of Málaga, Málaga, Spain.
    Robson, T Matthew
    University of Helsinki, Helsinki, Finland.
    Strid, Åke
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Beyond the visible: a handbook of best practice in plant UV photobiology2012Inngår i: Handbook for research on the effects of ultraviolet radiation on plants / [ed] Pedro J Aphalo, Helsingfors universitet, 2012, 1, s. 35-70Kapittel i bok, del av antologi (Fagfellevurdert)
    Abstract [en]

    The writing of this handbook started 14 months ago and is the results of the work of six editors and sixteen authors. This version is a preprint prepared for the participants in the 2012 training school of the COST action 5 FA0906 ‘UV4growth’ at University of Málaga. We hope that you find the handbook useful, and that you will alert us of errors, and of difficult to understand sections or paragraphs. Please, send all such comments to mailto:pedro.aphalo@helsinki.fi?subject=TG1HandbookPre01feedback indicating page and line numbers. Many thanks for your help.

  • 320.
    Perrigo, Allison L.
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    Romeralo, Maria
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    Baldauf, Sandra L.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    What's on your boots: an investigation into the role we play in protist dispersal2012Inngår i: Journal of Biogeography, ISSN 0305-0270, E-ISSN 1365-2699, Vol. 39, nr 5, s. 998-1003Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    D. M. Wilkinson (2010, Journal of Biogeography, 37, 393–397) suggested that anthropogenic dispersal is an understudied and potentially important factor in terrestrial protist biogeography. We investigated human footwear as a potential vector of dictyostelids, a diverse group of amoebae that includes both geographically restricted and cosmopolitan species. Eighteen pairs of boots were examined and dictyostelids were isolated from nearly all samples larger than 5.0 g. In total, six dictyostelid isolates were recovered, corresponding to four species –Dictyostelium minutum, D. sphaerocephalum, D. leptosomopsis and a new species, Polysphondylium sp. 1. Myxogastrid amoebae and acrasid-like aggregations were also observed. Thus anthropogenic dispersal of naked amoebae appears to occur. The possible role of variations in dictyostelid fruiting body morphologies in dispersal potential is also discussed. These results support Wilkinson’s proposal and suggest that dictyostelids may be a useful group with which to study anthropogenic dispersal of terrestrial protists.

  • 321.
    Perry, Diana
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Spatial risk assessment of global change impacts on Swedish seagrass ecosystemsManuskript (preprint) (Annet vitenskapelig)
  • 322.
    Persson, Erik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    När bina tystnar: Colony Collapse Disorder bakom honungsbiets (Apis mellifera) försvinnande2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Under vintrarna 2006/2007 och 2007/2008 vittnade man i USA om storskaliga förluster avhonungsbin (Apis mellifera). Gemensamma symptom för dessa förluster var att arbetarbinförsvann från kolonierna fastän det fanns rikligt med mat och inga tecken på infektion av någonparasit. Fenomenet fick namnet Colony Collapse Disorder (CCD) eftersom det slutgiltigastadiet var att kolonierna kollapsade. Denna översiktstudie ämnar till att reda ut begreppetCCD och se över vilka potentiella orsaker den rådande forskningen föreslår och vad man harkommit fram till sedan utbrottet 2006. Resultaten indikerar att det antagligen inte är en ensamfaktor bakom CCD utan att flera faktorer samverkar. Det finns ett antal hot mot bina medsymptom som liknar CCD och som antas bidra till försvinnandet. Ett hot är det parasitiskakvalstret Varroa destructor som förutom att det suger binas hemolymfa även agerar vektor åtflera CCD-förknippade patogener som Nosema ceranae och Israeli acute paralysis virus(IAPV). Dessutom sänker V. destructor binas immunförsvar vilket gör att virus som överförsvia kvalstret replikeras snabbare och blir dödligare. Man har även visat att vanligt förekommandebekämpningsmedel kan göra bina mer mottagliga för sjukdomar. Där hittade man ettsignifikant samband med just N. ceranae vilket är i enlighet med teorin om att flera faktorersamverkar. I nuläget bidrar CCD fortfarande till bidöd men är inte lika akut som 2006/2007.Istället är det andra hot såsom biodlares svårighet att behandla invasion av varroakvalster,kalla vintrar och att bisamhällen svälter ihjäl för att de invintras i för små populationer, som ärett större hot än CCD.

  • 323.
    Persson, Max
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Hur tillagning påverkar halterna av toxiska substanser i mat.2015Independent thesis Advanced level (degree of Master (Two Years)), 30 poäng / 45 hpOppgave
    Abstract [en]

    This study has examined how cooking affects the levels of the toxicantsaflatoxin, arsenic, lead, dioxins, cadmium, mercury, perfluorinatedcompounds and polybrominated diphenyl ethers in rice, potatoes and fish.Not every toxicant was examined for all three types of food, they were onlyexamined for those types of food where current levels of the toxicant in thattype of food are relevant from a risk assessment perspective. To determineif there is a danger of negative effects due to the exposure of thesetoxicants from food, articles from the Swedish National Food Agency andother scientific articles have been compiled. A minor experimentalsubstudy was also performed where rice bought in Uppsala was rinsed andboiled in different ways to see if that affected the residue levels of arsenic.The results of this study indicate that the levels of some toxic substancescan be lowered on a dry weight basis by cooking, and that this reduction isdependent on the cooking method used, the properties of the food andtoxin. The levels of cadmium and mercury were generally unchanged bycooking whereas the results for lead and perfluorinated compounds wereconflicting. Cooking can lover the levels of aflatoxin, arsenic, dioxins andpolybrominated diphenyl ethers on a dry weight basis. The effects ofcooking on toxicant levels should be considered when performing riskassessments, but further studies are needed to achieve a better basis for decision-making.

  • 324.
    Persson, Mia
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Trottier, Agaia J.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Bélteky, Johan
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Roth, Lina
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Jensen, Per
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Intranasal oxytocin and a polymorphism in the oxytocin receptor gene are associated with human-directed social behavior in golden retriever dogs2017Inngår i: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 95, s. 85-93Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The oxytocin system may play an important role in dog domestication from the wolf. Dogs have evolved unique human analogue social skills enabling them to communicate and cooperate efficiently with people. Genomic differences in the region surrounding the oxytocin receptor (OXTR) gene have previously been associated with variation in doge communicative skills. Here we have utilized the unsolvable problem paradigm to investigate the effects of oxytocin and OXTR polymorphisms on human-directed contact seeking behavior in 60 golden retriever dogs. Human-oriented behavior was quantified employing a previously defined unsolvable problem paradigm. Behaviors were tested twice in a repeated, counterbalanced design, where dogs received a nasal dose of either oxytocin or saline 45 min before each test occasion. Buccal DNA was analysed for genotype on three previously identified SNP-markers associated with OXTR. The same polymorphisms were also geno-typed in 21 wolf blood samples to explore potential genomic differences between the species. Results showed that oxytocin treatment decreased physical contact seeking with the experimenter and one of the three polymorphisms was associated with degree of physical contact seeking with the owner. Dogs with the AA-genotype at this locus increased owner physical contact seeking in response to oxytocin while the opposite effect was found in GG-genotype individuals. Hence, intranasal oxytocin treatment, an OXTR polymorphism and their interaction are associated with doge human-directed social skills, which can explain previously described breed differences in oxytocin response. Genotypic variation at the studied locus was also found in wolves indicating that it was present even at the start of dog domestication.

  • 325. Pierce, D. M.
    et al.
    Lilledahl, M. B.
    Ricken, T.
    De Lange Davies, C.
    Holzapfel, Gerhard A.
    KTH, Skolan för teknikvetenskap (SCI), Mekanik, Biomekanik. Graz University of Technology, Graz, Austria .
    Morphological analysis of articular cartilage using multiphoton microscopy as input for constitutive modeling: Experiment and mathematical implementation2010Inngår i: 6th World Congress Of Biomechanics (WCB 2010), Pts 1-3, Springer-Verlag New York, 2010, s. 895-898Konferansepaper (Fagfellevurdert)
    Abstract [en]

    The 3D structure of collagen fibers in chicken cartilage was quantified using multiphoton microscopy. Samples of fresh chicken cartilage were sectioned in three orthogonal planes using a vibratome. The sections were imaged using multiphoton microscopy, specifically imaging the collagen fibers using the second harmonic signal. Employing image analysis techniques based on Fourier analysis, the primary direction and anisotropy of the collagen fibers were extracted for the superficial layer resulting in a 3D map of the collagen fiber fabric. In the middle layer, image analysis using objective thresholding techniques was employed to extract the volume fraction occupied by extracellular matrix, the rest being occupied by the lacunae and residing chondrocytes. To implement these imaging data in a computational setting, we propose a new, 3D large strain constitutive model for articular cartilage, focused on the essential load-bearing morphology: an inhomogeneous, visco-poroelastic solid matrix reinforced by an anisotropic, viscoelastic dispersed fiber fabric which is saturated by an incompressible fluid residing in strain-dependent pores of the collagen-proteoglycan solid matrix. High-fidelity models, combining advanced imaging and computational biomechanics, will allow us to consider complex problems in structure-function relationships and provide insight to microphysical (mechanobiological) cellular stimuli.

  • 326.
    Pini Prato, Enrico
    et al.
    Aquaterra Associates, Florence, Italy.
    Comoglio, Claudio
    DITAG—Land, Environment and Geo-Engineering Department, Politecnico di Torino.
    Calles, Olle
    Karlstads universitet, Fakulteten för samhälls- och livsvetenskaper, Avdelningen för biologi.
    A simple management tool for planning the restoration of river longitudinalconnectivity at watershed level: priority indices for fish passes2011Inngår i: Journal of Applied Ichthyology, ISSN 0175-8659, E-ISSN 1439-0426, Vol. 27, nr suppl. s3, s. 73-79Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The present study aimed at defining and testing a simple and widely applicable management tool, based on already available or easily collectable data, which could allow Watershed Authorities to prioritize the interventions for river longitudinal connectivity restoration, reopening fish migration routes. The proposed priority indices for fish passes are based on obstacle characteristics, length of the potential reopened reach and fish species distribution and migratory behaviour. The indices were applied to two Italian watersheds (Arno and Magra Rivers) covering a total river length of more than 400 km. The priority lists produced by the application can function as a first step in defining a watershed restoration plan and could help the Watershed Authorities to address the available resources towards more detailed studies to be carried out at sites classified with the higher priority levels. This is the case of the Regional Natural Park Monte Marcello-Magra, where, within a recently funded LIFE+ project (P.A.R.C. –‘Petromyzon And River Continuity’), the first results of the priority indices application will be analyzed in greater detail and will consequently lead to the design and construction of fish passes to restore fish migration routes with a particular focus on sea lamprey (Petromyzon marinus).

  • 327. Pohl, J.
    et al.
    Björlenius, Berndt
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Industriell bioteknologi.
    Brodin, T.
    Carlsson, G.
    Fick, J.
    Larsson, D. G. J.
    Norrgren, L.
    Örn, S.
    Effects of ozonated sewage effluent on reproduction and behavioral endpoints in zebrafish (Danio rerio)2018Inngår i: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 200, s. 93-101Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Pharmaceutical residues and other micro-contaminants may enter aquatic environments through effluent from sewage treatment plants (STPs) and could cause adverse effects in wild fish. One strategy to alleviate this situation is to improve wastewater treatment by ozonation. To test the effectiveness of full-scale wastewater effluent ozonation at a Swedish municipal STP, the added removal efficiency was measured for 105 pharmaceuticals. In addition, gene expression, reproductive and behavioral endpoints were analyzed in zebrafish (Danio rerio) exposed on-site over 21 days to ozonated or non-ozonated effluents as well as to tap water. Ozone treatment (7 g O3/m3) removed pharmaceuticals by an average efficiency of 77% in addition to the conventional treatment, leaving 11 screened pharmaceuticals above detection limits. Differences in biological responses of the exposure treatments were recorded in gene expression, reproduction and behavior. Hepatic vitellogenin gene expression was higher in male zebrafish exposed to the ozonated effluent compared to the non-ozonated effluent and tap water treatments. The reproductive success was higher in fish exposed to ozonated effluent compared to non-ozonated effluent and to tap water. The behavioral measurements showed that fish exposed to the ozonated STP effluent were less active in swimming the first minute after placed in a novel vessel. Ozonation is a capable method for removing pharmaceuticals in effluents. However, its implementation should be thoroughly evaluated for any potential biological impact. Future research is needed for uncovering the factors which produced the in vivo responses in fish.

  • 328.
    Pohl, Johannes
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. Institutionen för biomedicin och veterinär folkhälsovetenskap, Department of Biomedical Sciences and Veterinary Public Health, Sveriges Lantbruksuniversitet, Swedish University of Agricultural Sciences.
    Thyroid Endocrine Disruption of Propylparaben Assessed Using an Optimized Individual Xenopus tropicalis Metamorphosing Tadpole Exposure System2015Independent thesis Advanced level (degree of Master (Two Years)), 30 poäng / 45 hpOppgave
    Abstract [en]

    The anuran Xenopus tropicalis tadpole is an attractive model animal in toxicological evaluation of suspected thyroid disrupting xenobiotics. Due to its reliance of a functioning hypothalamic-pituitary-thyroid (HPT) axis for normal metamorphosis, effects on the HPT axis produces apical endpoints, which are easy to measure. More sensitive endpoints of mRNA expression and histological evaluation of the thyroid gland itself provide strong indications of in vivo thyroid endocrine disruption. X. tropicalis is traditionally exposed in groups of 20 animals in four replicates for each treatment group. However, exposing tadpoles individually can provide stronger statistics and a reduction of total animal sacrifice. In this study we have developed and optimized an individual exposure system by a method development study. This method was then applied in an exposure experiment of a suspected thyroid endocrine disruptor, propylparaben (PrP). Prometamorphotic (NF stage 51) X. tropicalis tadpoles were distributed in three treatment groups (0.05, 0.5 and 5.0 mg PrP/L water) and maintained semi-statically for 14 days. Afterwards, apical measurements (body weight (BW), total body length (BL), snout to vent length (SVL) and hind limb length (HLL)) and reached developmental stage were assessed. In addition, mRNA expression of HPT axis relevant genes encoding deiodinase 2 (D2, hepatic tissue), deiodinase 3 (D3, hepatic and tail tissue) and transthyretin (Ttr, hepatic tissue) were measured by quantitative reverse transcription PCR (qRT-PCR). The PrP exposure did not affect general growth and development, but it did cause a downregulation of dio3 and ttr. The downregulation of dio3 could possibly be associated with a reduced serum content of thyroid hormone, while ttr might be connected to a previously described xenoestrogenic effect of PrP in vitro and in fish.

  • 329. Pohl, Johannes
    et al.
    Björlenius, Berndt
    Brodin, Tomas
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap.
    Carlsson, Gunnar
    Fick, Jerker
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
    Larsson, D. G. Joakim
    Norrgren, Leif
    Örn, Stefan
    Effects of ozonated sewage effluent on reproduction and behavioral endpoints in zebrafish (Danio rerio)2018Inngår i: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 200, s. 93-101Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Pharmaceutical residues and other micro-contaminants may enter aquatic environments through effluent from sewage treatment plants (STPs) and could cause adverse effects in wild fish. One strategy to alleviate this situation is to improve wastewater treatment by ozonation. To test the effectiveness of full-scale wastewater effluent ozonation at a Swedish municipal STP, the added removal efficiency was measured for 105 pharmaceuticals. In addition, gene expression, reproductive and behavioral endpoints were analyzed in zebrafish (Danio rerio) exposed on-site over 21 days to ozonated or non-ozonated effluents as well as to tap water. Ozone treatment (7 g O-3/m(3)) removed pharmaceuticals by an average efficiency of 77% in addition to the conventional treatment, leaving 11 screened pharmaceuticals above detection limits. Differences in biological responses of the exposure treatments were recorded in gene expression, reproduction and behavior. Hepatic vitellogenin gene expression was higher in male zebrafish exposed to the ozonated effluent compared to the non-ozonated effluent and tap water treatments. The reproductive success was higher in fish exposed to ozonated effluent compared to non-ozonated effluent and to tap water. The behavioral measurements showed that fish exposed to the ozonated STP effluent were less active in swimming the first minute after placed in a novel vessel. Ozonation is a capable method for removing pharmaceuticals in effluents. However, its implementation should be thoroughly evaluated for any potential biological impact. Future research is needed for uncovering the factors which produced the in vivo responses in fish.

  • 330.
    Porseryd, Tove
    et al.
    Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Miljövetenskap.
    Reyhanian Caspillo, Nasim
    Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi. Örebro universitet.
    Volkova, Kristina
    Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi. Örebro universitet.
    Elabbas, Lubna
    Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi.
    Källman, Thomas
    Uppsala university.
    Dinnétz, Patrik
    Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Miljövetenskap.
    Olsson, Per-Erik
    Örebro universitet.
    Porsch Hällström, Inger
    Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi.
    Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinyl estradiol2018Inngår i: General and Comparative Endocrinology, ISSN 0016-6480, E-ISSN 1095-6840, Vol. 262, s. 44-58Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    17α-Ethinylestradiol (EE2) is a ubiquitous aquatic contaminant shown to decrease fish fertility at low concentrations, especially in fish exposed during development. The mechanisms of the decreased fertility are not fully understood. In this study, we perform transcriptome analysis by RNA sequencing of testes from zebrafish with previously reported lowered fertility due to exposure to low concentrations of EE2during development. Fish were exposed to 1.2 and 1.6 ng/L (measured concentration; nominal concentrations 3 and 10 ng/L) of EE2 from fertilization to 80 days of age, followed by 82 days of remediation in clean water. RNA sequencing analysis revealed 249 and 16 genes to be differentially expressed after exposure to 1.2 and 1.6 ng/L, respectively; a larger inter-sample variation was noted in the latter. Expression of 11 genes were altered by both exposures and in the same direction. The coding sequences most affected could be categorized to the putative functions cell signalling, proteolysis, protein metabolic transport and lipid metabolic process. Several homeobox transcription factors involved in development and differentiation showed increased expression in response to EE2 and differential expression of genes related to cell death, differentiation and proliferation was observed. In addition, several genes related to steroid synthesis, testis development and function were differentially expressed. A number of genes associated with spermatogenesis in zebrafish and/or mouse were also found to be differentially expressed. Further, differences in non-coding sequences were observed, among them several differentially expressed miRNA that might contribute to testis gene regulation at post-transcriptional level. This study has generated insights of changes in gene expression that accompany fertility alterations in zebrafish males that persist after developmental exposure to environmental relevant concentrations of EE2 that persist followed by clean water to adulthood. Hopefully, this will generate hypotheses to test in search for mechanistic explanations.

  • 331.
    Posthouwer, Chantal
    et al.
    Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, The Netherlands; Institute of Biology Leiden, Leiden University, P.O. Box 9505, 2300 RA Leiden, The Netherlands.
    Veldman, Sarina
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi.
    Abihudi, Sarina
    Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, United Nations Road, P.O. Box 65001, Dar es Salaam, Tanzania.
    Otieno, Joseph N.
    Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, United Nations Road, P.O. Box 65001, Dar es Salaam, Tanzania.
    van Andel, Tinde R.
    Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, The Netherlands; Institute of Biology Leiden, Leiden University, P.O. Box 9505, 2300 RA Leiden, The Netherlands.
    de Boer, Hugo J.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för organismbiologi, Systematisk biologi. Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, The Netherlands; Natural History Museum, University of Oslo, Postboks 1172, Blindern, 0318 Oslo, Norway.
    Quantitative market survey of non-woody plants sold at Kariakoo Market in Dar es Salaam, Tanzania2018Inngår i: Journal of Ethnopharmacology, ISSN 0378-8741, E-ISSN 1872-7573, Vol. 222, s. 280-287Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Ethnopharmacological relevance: In Tanzania, traditional medicine plays a significant role in health care and local economies based on the harvesting, trade and sale of medicinal plant products. The majority of this plant ma- terial is said to originate from wild sources, and both traditional healers and vendors are concerned about the increasing scarcity of certain species.

    Aim of the study: A market survey of non-powdered, non-woody medicinal plants was conducted at Kariakoo Market in Dar es Salaam, the major hub for medicinal plant trade in Tanzania, to assess sustainability of traded herbal medicine. Materials and Methods: For this study, fresh and dried herbs, seeds and fruits were collected and interviews were conducted to obtain information on vernacular names, preparation methods, monthly sales, uses and prices. Bundles of herbal medicine offered for sale were weighed and counted to calculate the value and volumes of daily stock at the market.

    Results: A total of 71 medicinal plant products belonging to 62–67 different species from at least 41 different plant families were identified. We identified 45 plant products to species level, 20 products to genus level and four to family level. Plant species most encountered at the market were Suregada zanzibariensis, Myrothamnus flabellifolia and Sclerocarya birrea. The major use categories reported by the vendors were ritual purposes, di- gestive disorders and women's health. Annual sales are estimated to be in excess of 30 t and close to 200,000 USD, and trade in herbal medicine at Kariakoo Market provides subsistence income to many local vendors. Conclusions: A large diversity of wild-harvested plant species is traded as medicinal products in Tanzania, in- cluding species listed on CITES Appendices. Identifying and monitoring temporal changes in availability per season and from year to year will reveal which species are most affected by this trade, and help relevant au- thorities in Tanzania to find alternative sources of income for dependent stakeholders and initiate targeted efforts to protect threatened plant species.

  • 332.
    Prithviraj, Desale
    et al.
    Dr DY Patil Biotechnol & Bioinformat Inst, Dr DY Patil Vidyapeeth, Pune, India.
    Deboleena, Kashyap
    Dr DY Patil Biotechnol & Bioinformat Inst, Dr DY Patil Vidyapeeth, Pune, India.
    Neelu, Nawani
    Dr DY Patil Biotechnol & Bioinformat Inst, Dr DY Patil Vidyapeeth, Pune, India.
    Noor, Nahar
    University of Skövde, Skövde, Sweden.
    Aminur, Rahman
    University of Skövde, Skövde, Sweden.
    Balasaheb, Kapadnis
    Dept Microbiol, Univ Pune, Pune, India.
    Abul, Mandal
    University of Skövde, Skövde, Sweden.
    Biosorption of nickel by Lysinibacillus sp BA2 native to bauxite mine2014Inngår i: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 107, s. 260-268Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (-39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04 mg of Ni(II) adsorbed on one gram of dead biomass and 196.32 mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180 mm with rapid biosorption in initial 30 min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirrned by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.

  • 333.
    Rabiei Far, Parisa
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Study on cytotoxic activity of chloroformic fractions from Astraceae family on a number of cancer cell lines2013Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
    Abstract [en]

    Cancer is considered as one of the leading causes of death worldwide. The standard treatments of cancer are surgery, chemotherapy and radiation therapy. It is significant that a number of currently used anti-cancer agents are derived from natural sources, including plants, marine organisms and micro-organisms. In Iran, because of its climate diversity numerous varieties of plants can grow. Many of these plants such as Glycyrrhizaglabra, Foeniculumvulgare and Polygonumspecies have shown to possess anti-inflammatory and immunomodulatory effects. Asteraceae or Compositae family is famous for its tranquilizing effect, antifungal and cytotoxic activities. In frame of an ethnopharmacological project, local healers of some provinces of north east of Iran were interviewed by using questionnaire forms and could identify a number of herbs mainly from Astraceae family which are used for treatment traditionally in some areas of northern part of Iran. To screen the anti cancer effects of plants from this family, plants  were collected and extraction was done by using methanol maceration and finally extracts were tested for their toxicity toward a number of cancer cell lines by performing colorimetric cytotoxicity assay, extracts with high toxicity were sent for fractionation. The current study was aimed to identify the possible cytotoxic effect of two chloroformic fractions from Asteraceae family on four cancer cell lines (HepG2, HeLa, MN1 and MDD2) by using MTT colorimetric cytotoxicity assay. Results suggest that following 72 hours exposure, both fractions exhibited a substantial antiproliferative effect in all four tested cell lines. Moreover, concentration range for inducing 50% of cell death (IC50%) was determined. Our results point to a robust inhibitory effect of chloroformic fractions specifically toward HeLa cancer cell lines. These plants represent valuable resources for the development of potential anticancer agents.

  • 334.
    Rahman, Aminur
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. Örebro University.
    Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem2016Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

    In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

    The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

  • 335.
    Rahman, Aminur
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem2016Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

    In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

    The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

  • 336.
    Rahman, Aminur
    et al.
    Örebro universitet, Institutionen för naturvetenskap och teknik. University of Skövde.
    Nahar, Noor
    University of Skövde.
    Olsson, Björn
    University of Skövde.
    Jass, Jana
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Nawani, Neelu N.
    Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Tathawade, Pune-411033, India.
    Ghosh, Sibdas
    Iona College, New Rochelle, NY, USA.
    Saha, Ananda K.
    University of Rajshahi, Rajshahi, Bangladesh.
    Hossain, Khaled
    University of Rajshahi, Bangladesh.
    Mandal, Abul
    University of Skövde.
    Genome analysis of Enterobacter cloacae B2-DHA – A bacterium resistant to chromium and/or other heavy metalsManuskript (preprint) (Annet vitenskapelig)
  • 337.
    Rahman, Aminur
    et al.
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. Örebro universitet, Institutionen för naturvetenskap och teknik.
    Nahar, Noor
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Olsson, Björn
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Jass, Jana
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Nawani, Neelu N.
    Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Tathawade, Pune-411033, India.
    Ghosh, Sibdas
    Iona College, New Rochelle, NY, USA.
    Saha, Ananda K.
    University of Rajshahi, Rajshahi, Bangladesh.
    Hossain, Khaled
    University of Rajshahi, Bangladesh.
    Mandal, Abul
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi.
    Genome analysis of Enterobacter cloacae B2-DHA: A bacterium resistant to chromium and/or other heavy metalsInngår i: Genomics, ISSN 0888-7543, E-ISSN 1089-8646Artikkel i tidsskrift (Annet vitenskapelig)
  • 338.
    Rajangam, Alex S.
    KTH, Skolan för bioteknologi (BIO).
    Functional genomics of wood degradation and biosynthesis2005Licentiatavhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Forest biotechnology is a fast emerging field of research. The application of biotechnological tools will enhance the quality of the forest products. The resultant value added and environmentally sustainable products are an absolute necessity in the future. The study of wood biosynthesis and degradation will result in enormous knowledge resources, which can be used for exploiting wood properties. This thesis addresses questions representing both wood degradation and biosynthesis.

    The wood degrading fungus Phanerochaete chrysosporium is expression profiled with the microarray technology. The objective is to understand the expression pattern of the extracellular carbohydrate active enzymes (CAZymes) secreted by the organism. The data obtained increases our understanding of gene expression upon growth on cellulose.

    Wood biosynthesis is studied with the model wood forming tree species, Populus. The plentiful data resources from the expression profiling during wood formation in Populus are used as the platform of this work. One of the wood specific genes, PttMAP20, previously with an unknown function is studied in this thesis. The immunolocalisation of PttMAP20 with specific antibodies is demonstrated. The putative microtubule-targeting domain of the protein is demonstrated microscopically and by using a biochemical binding assay.

  • 339. Rams, Susana
    et al.
    Hedenäs, Lars
    Naturhistoriska riksmuseet, Enheten för botanik.
    Werner, Olaf
    Ros, Rosa María
    Nuevos datos sobre la presencia de Pseudotaxiphyllum laetevirens en España2014Inngår i: Boletín de la Sociedad Española de Briología, Vol. 42-43, s. 19-24Artikkel i tidsskrift (Annet vitenskapelig)
  • 340.
    Rasmusson, Lina M.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Seagrass Respiration: An assessment of oxygen consumption patterns of temperate marine macrophytes2015Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    In coastal seas, the abundance of marine macrophytes has profound influence on the flows of oxygen and inorganic carbon through the water. Vast amounts of carbon dioxide are taken up by photosynthesis and part of this is respired back into the water column. The photosynthetic carbon uptake of the most common seagrasses of the northern hemisphere is nowadays extensively studied at both community- and individual levels, and its impact on coastal carbon- and oxygen fluxes is quite well defined. However, the coinciding release of carbon dioxide and consumption of oxygen by the processes of mitochondrial respiration and photorespiration in these organisms has as yet not been given much attention, especially concerning how these processes are affected by external factors. For estimations of the rates of mitochondrial respiration, the common approach has been to use values obtained during darkness and treat them as being constant over the day. This approach is questioned in this thesis where the effects of different abiotic and biotic factors on oxygen consumption were examined to elucidate possible variations of seagrass respiration rates (with primary focus on the species Zostera marina), explored mainly using gas exchange techniques. The initial aim was to investigate whether the rates of respiration are at all fluctuating. This was found to be the case. Secondly, impacts of various factors on the respiration process were examined on Z. marina, with additional studies on the seagrass Ruppia maritima and the common green alga Ulva intestinalis. It was found that respiration rates were lower in the light for all three species. Specific investigations on Z. marina showed that respiration rates also varied with time of the day. Moreover, the rates of both respiration and photosynthesis differed between Z. marina shoots of different age as well as among different parts of the leaves. These differences were observed at both ambient (19.1oC) and elevated (29.1oC) temperatures. Photorespiration, previously considered insignificant in seagrasses, was found to have a profound role, as high rates were observed in productive bays, i.e. in settings with low inorganic carbon availability and high oxygen. Overall, this thesis has identified important external and developmental factors influencing the patterns of oxygen consumption and associated carbon dioxide release of two common temperate seagrasses. Clearly, respiration in seagrasses is a dynamic process that responds to a variety of external and developmental factors, which should be carefully considered when assessing the carbon budget of coastal vegetated areas.

  • 341.
    Rasmusson, Lina M.
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Björk, Mats
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Gullström, Martin
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Effects of increased temperature on respiration and photosynthesis differ among different leaf parts and with tissue age of the seagrass Zostera marinaManuskript (preprint) (Annet vitenskapelig)
  • 342.
    Rasmusson, Lina M.
    et al.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Lauritano, Chiara
    Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
    Procaccini, Gabriele
    Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
    Gullström, Martin
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Björk, Mats
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och botanik.
    Respiratory oxygen consumption in the seagrass Zostera marina is affected by light and varies on a diel basis: a combined gas exchange and gene expression studyManuskript (preprint) (Annet vitenskapelig)
  • 343. Ratke, Christine
    et al.
    Pawar, Prashant Mohan-Anupama
    Balasubramanian, Vimal K.
    Naumann, Marcel
    Duncranz, Mathilda Lönnäs
    Derba-Maceluch, Marta
    Gorzsas, Andras
    Endo, Satoshi
    Ezcurra, Ines
    KTH, Skolan för bioteknologi (BIO), Industriell bioteknologi.
    Mellerowicz, Ewa J.
    Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification2015Inngår i: Plant Biotechnology Journal, ISSN 1467-7644, E-ISSN 1467-7652, Vol. 13, nr 1, s. 26-37Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The plant GT43 protein family includes xylosyltransferases that are known to be required for xylan backbone biosynthesis, but have incompletely understood specificities. RT-qPCR and histochemical (GUS) analyses of expression patterns of GT43 members in hybrid aspen, reported here, revealed that three clades of the family have markedly differing specificity towards secondary wall-forming cells (wood and extraxylary fibres). Intriguingly, GT43A and B genes (corresponding to the Arabidopsis IRX9 clade) showed higher specificity for secondary-walled cells than GT43C and D genes (IRX14 clade), although both IRX9 and IRX14 are required for xylosyltransferase activity. The remaining genes, GT43E, F and G (IRX9-L clade), showed broad expression patterns. Transient transactivation analyses of GT43A and B reporters demonstrated that they are activated by PtxtMYB021 and PNAC085 (master secondary wall switches), mediated in PtxtMYB021 activation by an AC element. The high observed secondary cell wall specificity of GT43B expression prompted tests of the efficiency of its promoter (pGT43B), relative to the CaMV 35S (35S) promoter, for overexpressing a xylan acetyl esterase (CE5) or downregulating REDUCED WALL ACETYLATION (RWA) family genes and thus engineering wood acetylation. CE5 expression was weaker when driven by pGT43B, but it reduced wood acetyl content substantially more efficiently than the 35S promoter. RNAi silencing of the RWA family, which was ineffective using 35S, was achieved when using GT43B promoter. These results show the utility of the GT43B promoter for genetically engineering properties of wood and fibres.

  • 344.
    Remes Eriksson, Åsa
    Högskolan i Gävle, Akademin för teknik och miljö, Avdelningen för elektronik, matematik och naturvetenskap.
    Framtagandet av gestaltningsskiss i sjukhusmiljö med sten och stenpartiväxter som utgångsmaterial2014Independent thesis Basic level (university diploma), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Enligt forskning har riktig natur och bilder av natur en avstressande effekt på människor i sjukhusmiljö. Stenpartier byggs ofta för att simulera naturliga förhållanden och kan upplevas som en tredimensionell landskapsmålning som förändras med årstidens växlingar.

    Syftet med denna studie är att komma fram till en gestaltningsskiss med hjälp av sten och stenpartiväxter. Gestaltningen tänkta plats är utanför Gävle sjukhus. Fokus har lagts på stenpartiets uppbyggnad, växternas krav och anpassningen till sjukhusmiljön.

    För att ta reda på ett stenpartis uppbyggnad och stenpartiväxters krav på växtmiljö har både en litteraturstudie och platsbesök med intervjuer gjorts. Litteraturen är sökt via LIBRIS. Platsbesöken är gjorda i två svenska botaniska trädgårdar där personer med expertkunskaper i ämnet intervjuats. För att få gestaltningens utformning att passa in i en sjukhusmiljö är en litteraturstudie gjord. Litteraturstudien undersöker vad som bör undvikas och vad som rekommenderas. Litteraturen är sökt i databasen Discovery med sökorden hospital and art. För att ta reda på platsens fysiska förutsättningar har en platsobservation gjorts.

    Ett stenparti som liknar naturen passar bra in i sjukhusmiljön med tanke på att bilder av natur ger positiva känslor. Fördelen med stenpartiet är att bilden av natur blir verklig och kan upplevas av fler sinnen. Om stenpartiet inte sköts blir effekten motsatt. Skötseln är som mest intensiv de första åren men avtar då växterna väl etablerat sig. För att reducera framtida skötselbehov bör växter väljas efter klimatet och platsens förutsättningar. Detta resulterade i att många inhemska växter passade bra till gestaltningsförslaget. I en orolig sjukhusmiljö kan inhemska växter få människor att känna sig hemma. Viktigt är också att välja mattbildande växter som hindrar ogräs från att etablera sig.

    Erfarenhet av växter, anläggning och skötsel är avgörande om ett stenparti ska kunna bli till bilden av natur.

  • 345.
    Revonsuo, Antti
    et al.
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. Turun yliopisto, Turku, Finland.
    Tuominen, Jarno
    Turun yliopisto, Turku, Finland.
    Valli, Katja
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. Turun yliopisto, Turku, Finland.
    The Avatars in the Machine: Dreaming as a Simulation of Social Reality2016Inngår i: Open MIND: Philosophy and the Mind Sciences in the 21st Century / [ed] Thomas Metzinger & Jennifer M. Windt, MIT Press, 2016, s. 1295-1322Kapittel i bok, del av antologi (Fagfellevurdert)
  • 346.
    Revonsuo, Antti
    et al.
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. Turun yliopisto, Turku, Finland.
    Tuominen, Jarno
    Turun yliopisto, Turku, Finland.
    Valli, Katja
    Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. Turun yliopisto, Turku, Finland.
    The Simulation Theories of Dreaming: How to Make Theoretical Progress in Dream Science2016Inngår i: Open MIND: Philosophy and the Mind Sciences in the 21st Century / [ed] Thomas Metzinger & Jennifer M. Windt, MIT Press, 2016, s. 1341-1348Kapittel i bok, del av antologi (Fagfellevurdert)
  • 347.
    Reyhanian Caspillo, Nasim
    et al.
    Örebro universitet, Institutionen för naturvetenskap och teknik. Södertörn university.
    Porseryd, Tove
    Södertörn university.
    Volkova, Kristina
    Örebro universitet, Institutionen för naturvetenskap och teknik. Södertörn university.
    Elabbas, Lubna
    Södertörn university.
    Källman, Thomas
    Uppsala university.
    Dinnétz, Patrik
    Södertörn university.
    Olsson, Per-Erik
    Örebro universitet, Institutionen för naturvetenskap och teknik.
    Porsch Hällström, Inger
    Södertörn university.
    Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinyl estradiolManuskript (preprint) (Annet vitenskapelig)
  • 348.
    Reyhanian, Nasim
    et al.
    Södertörns Högskola.
    Volkova, Kristina
    Södertörns Högskola.
    Hallgren, Stefan
    Södertörns Högskola.
    Bollner, Tomas
    Södertörns Högskola.
    Olsson, Per-Erik
    Örebro Universitet.
    Olsén, Håkan
    Södertörns Högskola.
    Porsch-Hällström, Inger
    Södertörns Högskola.
    17α-ethynyl estradiol affect anxiety and shoaling behavior in adult zebra fish (Danio rerio).2011Inngår i: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 104, nr 1-2, s. 41-48Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Ethinyl estradiol is a potent endocrine disrupting compound in fish and ubiquitously present in the aquatic environment. In this study, we exposed adult zebra fish (Danio rerio) males to 0, 5 or 25 ng Ethinyl estradiol/L for 14 days and analyzed the effects on non-reproductive behavior. Effects of treatment of the exposed males was shown by vitellogenin induction, while brain aromatase (CYP 19B) activity was not significantly altered. Both concentrations of Ethinyl estradiol significantly altered the behavior in the Novel tank test, where anxiety is determined as the tendency to stay at the bottom when introduced into an unfamiliar environment. The effects were, however, opposite for the two concentrations. Fish that were exposed to 5 ng/L had longer latency before upswim, fewer transitions to the upper half and shorter total time spent in the upper half compared with control fish, while 25 ng Ethinyl estradiol treatment resulted in shorter latency and more and longer visits to the upper half. The swimming activity of 25, but not 5 ng-exposed fish were slightly but significantly reduced, and these fish tended to spend a lot of time at the surface. We also studied the shoaling behavior as the tendency to leave a shoal of littermates trapped behind a Plexiglas barrier at one end of the test tank. The fish treated with Ethinyl estradiol had significantly longer latency before leaving shoal mates and left the shoal fewer times. Further, the fish exposed to 5 ng/L also spent significantly less time away from shoal than control fish. Fertilization frequency was higher in males exposed to 5 ng/L Ethinyl estradiol when compared with control males, while no spawning was observed after treatment with 25 ng/L. The testes from both treatment groups contained a normal distribution of spermatogenesis stages, and no abnormality in testis morphology could be observed.

    In conclusion, we have observed effects on two behaviors not related to reproduction in zebra fish males after treatment with Ethinyl estradiol, adding to the ecological consequences of contamination of aquatic environments with estrogenic substances.

  • 349.
    Ritenberga, Olga
    et al.
    University of Latvia Faculty of Geography and Earth Sciences.
    Sofiev, Mikhail
    Finnish Meteorological Institute.
    Siljamo, Pilvi
    Finnish Meteorological Institute.
    Saarto, Annika
    Unit of Aerobiology, University of Turku.
    Dahl, Aslog
    Department of Biological and Environmental Sciences, University of Gothenburg.
    Ekebom, Agneta
    Naturhistoriska riksmuseet, Enheten för miljöforskning och övervakning.
    Sauliene, Ingrida
    Research Institute, Siauliai University.
    Shalaboda, Valentina
    Institute for Experimental Botany of the NAS of Belarus.
    Severova, Elena
    Moscow State University.
    Hoebeke, Lucie
    Belgian Aerobiological Network, Mycology and Aerobiology service, Scientific Institute of Public Health.
    Ramfjord, Hallvard
    Department of Biology, Norwegian University of Science and Technology.
    A statistical model for predicting the inter-annual variability of birchpollen abundance in Northern and North-Eastern Europe2018Inngår i: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 615, s. 228-239Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The paper suggests amethodology for predicting next-year seasonal pollen index (SPI, a sumof daily-mean pollen concentrations)over large regions and demonstrates its performance for birch in Northern andNorth-Eastern Europe. Astatistical model is constructed using meteorological, geophysical and biological characteristics of the previous year).A cluster analysis of multi-annual data of European Aeroallergen Network (EAN) revealed several large regions inEurope, where the observed SPI exhibits similar patterns of the multi-annual variability.We built the model for thenorthern cluster of stations, which covers Finland, Sweden, Baltic States, part of Belarus, and, probably, Russia andNorway,where the lack of data did not allow for conclusive analysis. The constructed modelwas capable of predictingthe SPI with correlation coefficient reaching up to 0.9 for somestations, odds ratio is infinitely high for 50% of sites insidethe region and the fraction of prediction fallingwithin factor of 2 from observations, stays within 40–70%. In particular,model successfully reproduced both the bi-annual cycle of the SPI and years when this cycle breaks down.

  • 350.
    Robin, Libby
    et al.
    KTH, Skolan för arkitektur och samhällsbyggnad (ABE), Filosofi och teknikhistoria, Teknik- och vetenskapshistoria.
    Moore, Joslyn
    Willoughby, Sharon
    Maroske, Sara
    Aliens from the Garden2011Inngår i: Proceedings of the State of Australian Cities Conference: Melbourne 30 November-2 December 2011, 2011Konferansepaper (Fagfellevurdert)
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