Endre søk
Begrens søket
3456789 251 - 300 of 1389
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Treff pr side
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
Merk
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 251.
    Christoffersson, Jonas
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
    Meier, Florian
    Boehringer Ingelheim Pharma GmbH and Co. KG, Nonclinical Drug Safety Germany, D-88397 Biberach an der Riss, Germany.
    Kempf, Henning
    Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
    Schwanke, Kristin
    Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
    Coffee, Michelle
    Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
    Beilmann, Mario
    Boehringer Ingelheim Pharma GmbH and Co. KG, Nonclinical Drug Safety Germany, D-88397 Biberach an der Riss, Germany.
    Zweigerdt, Robert
    Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
    Mandenius, Carl-Fredrik
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
    A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device2018Inngår i: Bioengineering, E-ISSN 2306-5354, Vol. 5, nr 2, s. 1-13, artikkel-id 36Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Three-dimensional (3D) models with cells arranged in clusters or spheroids have emerged as valuable tools to improve physiological relevance in drug screening. One of the challenges with cells cultured in 3D, especially for high-throughput applications, is to quickly and non-invasively assess the cellular state in vitro. In this article, we show that the number of cells growing out from human induced pluripotent stem cell (hiPSC)-derived cardiac spheroids can be quantified to serve as an indicator of a drug’s effect on spheroids captured in a microfluidic device. Combining this spheroid-on-a-chip with confocal high content imaging reveals easily accessible, quantitative outgrowth data. We found that effects on outgrowing cell numbers correlate to the concentrations of relevant pharmacological compounds and could thus serve as a practical readout to monitor drug effects. Here, we demonstrate the potential of this semi-high-throughput “cardiac cell outgrowth assay” with six compounds at three concentrations applied to spheroids for 48 h. The image-based readout complements end-point assays or may be used as a non-invasive assay for quality control during long-term culture.

  • 252.
    Claesson, Cim
    Högskolan i Skövde, Institutionen för vård och natur.
    Is Multiplex Ligation-dependent Probe Amplification a good method for screening formalin fixed paraffin embedded neuroblastoma tumors?2011Independent thesis Basic level (degree of Bachelor), 20 poäng / 30 hpOppgave
    Abstract [en]

    Neuroblastoma is one of the most enigmatic solid tumors for scientists and pediatric oncologists. Neuroblastoma is primary a childhood form of cancer, consisting of neuroectodermal cells that originate from the neural crest and is destined for the  adrenal medulla and sympathetic nervous system. The Neuroblastoma group at The University of Gothenburg received formalin-fixed paraffin-embedded  tumor samples from Vietnam and this project was to examine if the quality of the DNA from, is good enough to run comparative genome hybridization array experiment  on by using a cheaper technique Multiplex  Ligation-dependent Probe Amplification   technique. Multiplex Ligation-dependent Probe Amplification (MRC Holland) is a multiplex PCR method that can detect abnormalities such as deletions and amplifications. By using probes consisting of one short synthetic arm with a PCR primer sequence Y at the 3´end, and one long probe with a stuffer sequence, and a PCR primer sequence X at the 5´end that can hybridize and ligate. If these probes ligate it is possible to amplify them by PCR just using specific primers for the X and Y sequences. The resulting amplification products can then be analyzed bycapillary electrophoresis. These patient that the DNA was derived from had all stage 4  neuroblastoma, and that is why they all present many aberrations. Among the fascinating data from this experiment, there are many patients with both 11q  deletions and has an extreme amplification of MYCN. In Sweden only a few cases has been discovered. In this material even though all patients are stage 4 patients, 16 have this combination.   

  • 253.
    Claesson, Terese
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
    Optimization of immunofluorence-based methods to detect anti-nuclear antibodies2015Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 254.
    Claesson, Thomas
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Bärextrakts effekt på trombocytfunktion: Nyuppsatt metod med helblodsaggregometri2013Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 255.
    Classon, Lisa
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Atypiskt terminalt komplementkomplex: Kvantifiering av in vivo-nivåer av atypiskt terminalt komplementkomplex under normala och patofysiologiska betingelser2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Slutsteget i immunförsvarets komplementaktivering innefattar en klyvning av komplementprotein C5, till C5a och C5b, vilket initierar bildandet av terminalt komplementkomplex (TCC) som i form av membran-attack-komplex (MAC) bildar cytotoxiska porer i bland annat gramnegativa bakterier. Bildandet av MAC kan blockeras av endogena regulatorer och TCC frisätts då som ett lösligt komplex, sC5b-9, i plasma. I examensarbetet studerades en variant av TCC, som i tidigare studier visats bildas oberoende av C3- och C5-konvertas när serum surgjorts till pH < 7,0 in vitro. Syftet med studien var att studera om detta atypiska TCC (aTCC) bildades hos grisar, som i en mekonium aspirationssyndrom (MAS)-modell, erhållit ett sänkt systemiskt pH in vivo. I syftet ingick också att etablera en ELISA-baserad metod för att analysera aTCC. I en sandwich ELISA användes monoklonal anti-C5a/C5a (desArg) (klon T13/9) som fångande antikropp och monoklonal anti-C9 (klon aE11) som detekterande antikropp för att analysera aTCC i plasmaprover från 18 MAS-grisar, samt i ett kontrollmaterial bestående av grisserum som surgjorts till pH 6,8 och 6,4 in vitro. Mängden aTCC i kontrollproverna ökade när pH sänktes men innehållet av aTCC i plasmaproverna minskade över MAS-studiens förlopp. När den relativa förändringen i aTCC relaterades till MAS-grisarnas slutliga pH kunde ett signifikant samband ses (p = 0,02) som visade att en större förändring i aTCC sammanföll med ett lägre slutligt pH. Nivåerna av aTCC var generellt sett högre i plasmaproverna jämfört med kontrollproverna vilket skulle kunnat bero på skillnader i plasma vs serum avseende aTCC eller att proverna kom från grisar med olika ålder och vikt. Avsaknad av grisspecifik standard och negativ kontroll samt lågt signal/brusförhållande bidrar till felkällor för analysen och denna kräver fortsatt optimering.

  • 256.
    Clausson, Carl-Magnus
    Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg.
    Making Visible the Proximity Between Proteins2014Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. The proteins take part in most, if not all, cellular processes, and it is by unraveling these processes that we can begin to understand life and disease-causing mechanisms.

    In vitro and in vivo assays are two levels at which protein communication may be studied, and which permit manipulation and control over the proteins under investigation. But in order to retrieve a representation of the processes as close to reality as possible, in situ analysis may instead be applied as a complement to the other two levels of study. In situ PLA offers the ability to survey protein activity in tissue samples and primary cell lines, at a single cell level, detecting single targets in their natural unperturbed environment.  

    In this thesis new developments of the in situ PLA are described, along with a new technique offering in situ enzyme-free detection of proximity between biomolecules.

    The dynamic range of in situ PLA has now been increased by several orders of magnitude to cover analogous ranges of protein expression; the output signals have been modified to offer a greater signal-to-noise ratio and to limit false-positive-rates while also extending the dynamic range further; simultaneous detection of multiple protein complexes is now possible; proximity-HCR is presented as a robust and inexpensive enzyme-free assay for protein complex detection.

    The thesis also covers descriptions on how the techniques may be simultaneously applied, also together with other techniques, for the multiple data-point acquisition required by the emerging realm of systems biology. A future perspective is presented for how much more information may be simultaneously acquired from tissue samples to describe biomolecular interactions in a new manner. This will allow new types of biomarkers and drugs to be discovered, and a new holistic understanding of life.

  • 257.
    Cocha, Laura Romina
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Metodutveckling för prövning av lektiners påverkan på patogena svampar2015Independent thesis Basic level (professional degree), 180 hpOppgave
  • 258. Cornes, Michael
    et al.
    van Dongen-Lases, Edmée
    Grankvist, Kjell
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Klinisk kemi.
    Ibarz, Mercedes
    Kristensen, Gunn
    Lippi, Giuseppe
    Nybo, Mads
    Simundic, Ana-Maria
    Order of blood draw: Opinion Paper by the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE)2017Inngår i: Clinical Chemistry and Laboratory Medicine, ISSN 1434-6621, E-ISSN 1437-4331, Vol. 55, nr 1, s. 27-31Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    It has been well reported over recent years that most errors within the total testing process occur in the pre-analytical phase (46%-68.2%), an area that is usually outside of the direct control of the laboratory and which includes sample collection (phlebotomy). National and international (WHO, CLSI) guidelines recommend that the order of draw of blood during phlebotomy should be blood culture/sterile tubes, then plain tubes/gel tubes, then tubes containing additives. This prevents contamination of sample tubes with additives from previous tubes that could cause erroneous results. There have been a number of studies recently looking at whether order of draw remains a problem with modern phlebotomy techniques and materials, or it is an outdated practice followed simply because of historical reasons. In the following article, the European Federation of Clinical Chemistry and Laboratory Medicine Working Group for the Preanalytical Phase (EFLM WG-PRE) provides an overview and summary of the literature with regards to order of draw in venous blood collection. Given the evidence presented in this article, the EFLM WG-PRE herein concludes that a significant frequency of sample contamination does occur if order of draw is not followed during blood collection and when performing venipuncture under less than ideal circumstances, thus putting patient safety at risk. Moreover, given that order of draw is not difficult to follow and knowing that ideal phlebotomy conditions and protocols are not always followed or possible, EFLM WG-PRE supports the continued recommendation of ensuring a correct order of draw for venous blood collection.

  • 259.
    Cornet, Ronald
    et al.
    Linköpings universitet, Institutionen för medicinsk teknik, Avdelningen för medicinsk teknik. Linköpings universitet, Tekniska fakulteten. Univ Amsterdam, Netherlands.
    Hill, Carly
    Univ Amsterdam, Netherlands.
    de Keizer, Nicolette
    Univ Amsterdam, Netherlands.
    Comparison of Three English-to-Dutch Machine Translations of SNOMED CT Procedures2017Inngår i: MEDINFO 2017: PRECISION HEALTHCARE THROUGH INFORMATICS, IOS PRESS , 2017, Vol. 245, s. 848-852Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Dutch interface terminologies are needed to use SNOMED CT in the Netherlands. Machine translation may support in their creation. The aim of our study is to compare different machine translations of procedures in SNOMED CT. Procedures were translated using Google Translate, Matecat, and Thot. Google Translate and Matecat are tools with large but general translation memories. The translation memory of Thot was trained and tuned with various configurations of a Dutch translation of parts of SNOMED CT, a medical dictionary and parts of the UMLS Metathesaurus. The configuration with the highest BLEU score, representing closeness to human translation, was selected. Similarity was determined between Thot translations and those by Google and Matecat. The validity of translations was assessed through random samples. Google and Matecat translated similarly in 85.4% of the cases and generally better than Thot. Whereas the quality of translations was considered acceptable, machine translations alone are yet insufficient.

  • 260.
    Croce, Daniel
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk biokemi och mikrobiologi. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa, Obstetrik & gynekologi.
    Investigation of Syndecan-1 Ectodomain Isolated from Chinese Hamster Ovary (CHO) Cell Culture Medium2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    Syndecan-1 is a cell surface proteoglycan which participates in cell adhesion, differentiation, motility, morphogenesis and intracellular signaling. The two glycosaminoglycans heparan sulfate and chondroitin sulfate are covalently attached to the ectodomain of syndecan-1 via a tetra saccharide linkage sequence. However, the ectodomain can be modified having only one or neither of the glycosaminglycans attached. The glycosaminoglycans are capable of binding ligands such as fibroblast growth factors (FGFs) and support activation of receptors. The ectodomain is proteolytically cleaved from the cell surface by metalloproteinases in a process known as shedding. Shedding turns the ectodomain into a soluble effector which can stimulate other cells in the surroundings by delivering growth factors and also translocate into cells through endocytosis. In this study the aim was to find out if a modified ectodomain, which only contains chondroitin sulfate, could support intracellular signaling in the absence of heparan sulfate. The aim was also to find out whether a modified ectodomain could translocate into the cell. The methods used were cell culturing, isolation and purification of syndecan-1 ectodomain, cell signaling and immunohistochemistry. It was found that modified shed syndecan-1 ectodomain was able to support intracellular signaling almost to the same degree as wild type syndecan-1 ectodomain. This may suggest that heparan sulfate does not have to be present on the ectodomain to support intracellular signaling, although the signal is slightly higher when present. When trying to detect translocation of the ectodomain the results were too uncertain and further research is required.

  • 261.
    Cronskär, Marie
    et al.
    Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Avdelningen för kvalitetsteknik, maskinteknik och matematik.
    Bäckström, Mikael
    Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Avdelningen för kvalitetsteknik, maskinteknik och matematik.
    Modeling of fractured clavicles and reconstruction plates using CAD, finite element analysis and real musculoskeletal forces input2013Inngår i: WIT Transactions on Biomedicine and Health, WIT Press, 2013, s. 235-243Konferansepaper (Fagfellevurdert)
    Abstract [en]

    This study focuses on the treatment options for clavicle fractures, more specifically the cases with a need for internal fixation: non-unions and some complex fractures. Enhancing the understanding of the loading of the bone and fixation device enables treatment options to be improved. The aim of the study was to develop a method for the realistic simulation of stresses and displacements in the bone and fixation device and to use this method to make comparisons between a conventional reconstruction plate and a customized plate, designed from patient-specific computed tomography (CT) data. In an earlier study, a finite element (FE) mesh of the clavicle geometry was created from CT data, subjected to muscle forces and other boundary conditions from a multibody musculoskeletal model and imported into the FE solver. In this study, a solid 3D model of the same clavicle geometry was created and the mesh was replaced by the solid model to make the FE-model more suitable for the comparison of different plates. An LCP Reco-Plate 3.5 straight, 6 holes (by Synthes) was compared with a customized plate which was designed to follow the anatomy of the bone. The LCP-Reco plate has tapered reconstruction segments throughout the plate to allow for the plate reshaping during surgery. The customized plate was designed without such segments and with a lower width than the LCP plate. The two different plates showed stresses and displacements of similar magnitudes. The customized plate had a more even stress distribution while the LCP plate had higher stress concentrations in the middle of the plate and on the edges of the tapered reconstruction segments. To the authors' best knowledge, this is the first FE model of a clavicle bone with plate and it may, upon further development, serve as a useful instrument for improved clavicle fixation.

  • 262.
    Cronskär, Marie
    et al.
    Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Institutionen för teknik och hållbar utveckling.
    Rännar, Lars-Erik
    Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Institutionen för teknik och hållbar utveckling.
    Bäckström, Mikael
    Mittuniversitetet, Fakulteten för naturvetenskap, teknik och medier, Institutionen för teknik och hållbar utveckling.
    Implementation of digital design and solid free-form fabrication for customization of implants in trauma orthopaedics2012Inngår i: Journal of medical and biological engineering, ISSN 1609-0985, Vol. 32, nr 2, s. 91-96Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Bone plates for the fixation of complex fractures in proximity to joints often have to be reshaped to follow the bone contour. Good adhesion of the screws in areas where the bone is osteoporotic is also a challenge. One possible solution to these issues is to tailor-make plates by creating a digital three-dimensional model of the fracture from a computed tomography (CT) scan, digitally reducing the fracture, designing a plate, and finally manufacturing it directly from the digital model with solid free-form fabrication (SFF) technology. This study designs a custom plate for a distal tibia fracture, and investigates and refines the procedure from the CT scan to the final implant, with the aim of making it usable in trauma orthopaedics. The bone plate is manufactured using electron beam melting (EBM) technology. The challenges of bone plate design using digitalization and SFF are discussed. The virtual models created by the engineer while digitally reducing the fracture and modeling the plate are valuable for the physician while planning the surgery. A combination of surgery planning and digital plate design improves the surgeon's preparations and ensures correspondence between the plan and the designed implant. The proposed procedure, with the approximate required time in brackets, includes the separation of bone in the DICOM file (60 min), the reduction of fracture (5-30 min), revision (30 min), modelling of the plate (30-120 min), confirmation (30 min), manufacturing with SFF (10 h), post-processing (60 min), and finally cleaning and sterilization (90 min). The whole procedure requires about three working days.

  • 263.
    Cros, Olivier
    Linköpings universitet, Institutionen för medicinsk teknik. Linköpings universitet, Tekniska fakulteten.
    Structural properties of the mastoid using image analysis and visualization2017Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The mastoid, located in the temporal bone, houses an air cell system whose cells have a variation in size that can go far below current conventional clinical CT scanner resolution. Therefore, the mastoid air cell system is only partially represented in a CT scan. Where the conventional clinical CT scanner lacks level of minute details, micro-CT scanning provides an overwhelming amount of ne details. The temporal bone being one of the most complex in the human body, visualization of micro-CT scanning of this boneawakens the curiosity of the experimenter, especially with the correct visualization settings.

    This thesis first presents a statistical analysis determining the surface area to volume ratio of the mastoid air cell system of human temporal bone, from micro-CT scanning using methods previously applied for conventional clinical CT scans. The study compared current results with previous studies, with successive downsampling the data down to a resolution found in conventional clinical CT scanning. The results from the statistical analysis showed that all the small mastoid air cells, that cannot be detected in conventional clinical CT scans, do heavily contribute to the estimation of the surface area, and in consequence to the estimation of the surface area to volume ratio by a factor of about 2.6. Such a result further strengthens the idea of the mastoid to play an active role in pressure regulation and gas exchange.

    Discovery of micro-channels through specific use of a non-traditional transfer function was then reported, where a qualitative and a quantitative pre-analysis were performed and reported. To gain more knowledge about these micro-channels, a local structure tensor analysis was applied where structures are described in terms of planar, tubular, or isotropic structures. The results from this structural tensor analysis suggest these microchannels to potentially be part of a more complex framework, which hypothetically would provide a separate blood supply for the mucosa lining the mastoid air cell system.

    The knowledge gained from analysing the micro-channels as locally providing blood to the mucosa, led to the consideration of how inflammation of the mucosa could impact the pneumatization of the mastoid air cell system. Though very primitive, a 3D shape analysis of the mastoid air cell system was carried out. The mastoid air cell system was first represented in a compact form through a medial axis, from which medial balls could be used. The medial balls, representative of how large the mastoid air cells can be locally, were used in two complementary clustering methods, one based on the size diameter of the medial balls and one based on their location within the mastoid air cell system. From both quantitative and qualitative statistics, it was possible to map the clusters based on pre-defined regions already described in the literature, which opened the door for new hypotheses concerning the effect of mucosal inflammation on the mastoid pneumatization.

    Last but not least, discovery of other structures, previously unreported in the literature, were also visually observed and briefly discussed in this thesis. Further analysis of these unknown structures is needed.

  • 264.
    Curiche, Natalia
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Visualization of Propionibacterium acnes in Patients Diagnosed with Acne Vulgaris. - Propionibacterium acnes Detected with Immuno­fluorescence and Fluorescence in situ Hybridization.2011Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 265.
    Dadarman, Mina
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Möjlig mekanism bakom antiinflammatorisk polymer, polyvinylalkoholkarbazat: Studier av apoptos och viabilitet i cellkultur2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 266.
    Dagsberg, Jacob
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Förekomst av aviärt influensavirus bland vilda fåglar provtagna vid Ottenby fågelstation under 2017: Detektion och fylogenetisk analys av neuraminidas subtyp 6-virus2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Aviärt influensavirus (AIV) delas in i olika subtyper baserat på två glykoprotein på ytan. Hos fåglar finns 16 subtyper av hemagglutinin (H) och nio subtyper av neuraminidas (N). Vissa H-subtyper kan mutera till högpatogena virus som orsakar dödliga infektioner. Ett sådant högpatogent H5N6-virus rapporterades i norra Europa under 2017. Utbredning av H5 från högpatogena AIV är väl kartlagda. För detektion av neuraminidas hos AIV används omvänd transkription följt av realtids polymeraskedjereaktion (RT-qPCR) genom syntes av komplementärt DNA följt av amplifiering och detektion med fluorescerande prob. Sangersekvensering av neuraminidaskodande gensegment används för att verifiera resultat från RT-qPCR-screening samt för analys av fylogenetisk släktskap. För att bidra med information om neuraminidas subtyp 6 utfördes RT-qPCR-analys på 282 AIV-positiva prover från vilda fåglar, provtagna vid Ottenby fågelstation under 2017. Gensegmenten som kodade för hemaglutinin och neuraminidas hos virus i N6-positiva prov sekvenserades för att validera resultaten från N6-RT-qPCR, bestämma H-subtyp och för att analysera fylogenetisk släktskap mellan N6-sekvenser. RT-qPCR resulterade i 37 N6-positiva prover där 26 av dessa konfirmerades som N6 med traditionell PCR och sekvensering. AIV från analysen förekom i subtypkonstellationerna: 14 H1N6, tre H3N6, åtta H4N6 och ett H5N6. Tre prover gav ingen H-subtyp, tre andra prover gav ingen N6-sekvens. Detekterad H5N6 var lågpatogen. Den fylogenetiska analysen visade att AIV N6-sekvenser från Ottenby, tillsammans med sekvenser från GenBank, bildade fyra olika genotyper. N6-sekvenser från två av virusen från denna studien, tillhörande subtyperna H3N6 och H1N6 i bildade genotyp två tillsammans med fyra H5N6-virus från Japan som möjligen är högpatogena.

  • 267.
    Dahlberg, Ida
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Förekomsten av Propionibacterium acnes är låg hos patienter med Rosacea: En studie av sambandet mellan Propionibacterium acnes och Rosacea med immunofluorescens2011Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 268.
    Dahlqvist Leinhard, Olof
    et al.
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa. Linköpings universitet, Hälsouniversitetet.
    Johansson, Andreas
    Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Rydell, Joakim
    Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV.
    Kihlberg, Johan
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för medicin och hälsa. Östergötlands Läns Landsting, Diagnostikcentrum, Röntgenkliniken i Linköping.
    Smedby, Örjan
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Medicinsk radiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Bildmedicinskt centrum, Röntgenkliniken i Linköping.
    Nyström, Fredrik H.
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa. Linköpings universitet, Hälsouniversitetet.
    Lundberg, Peter
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Medicinsk radiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Bildmedicinskt centrum, Röntgenkliniken i Linköping.
    Borga, Magnus
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Quantification of abdominal fat accumulation during hyperalimentation using MRI2009Inngår i: Proceedings of the ISMRM Annual Meeting (ISMRM'09), 2009, Berkeley, CA, USA: International Society for Magnetic Resonance in Medicine , 2009, s. 206-Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    There is an increasing demand for imaging methods that can be used for automatic, accurate and quantitative determination of the amounts of abdominal fat. Such methods are important as they will allow the evaluation of some of the risk factors underlying the ’metabolic syndrome’. The metabolic syndrome is becoming common in large parts of the world, and it appears that a dominant risk factor for developing this syndrome is abdominal obesity. Subjects that are afflicted with the metabolic syndrome are exposed to a high risk for developing a large range of diseases such as type 2 diabetes, cardiac failure, and stroke. The aim of this work

  • 269. Dahlström, Märta
    et al.
    Forsström, Daniel
    Johannesson, Malin
    Huque-Andersson, Yasmin
    Björk, Marie
    Silfverplatz, Erik
    Sanin, Andrei
    Schaal, Wesley
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för farmaceutisk biovetenskap.
    Pelcman, Benjamin
    Forsell, Pontus K A
    Development of a fluorescent intensity assay amenable for high-throughput screening for determining 15-lipoxygenase activity.2010Inngår i: Journal of Biomolecular Screening, ISSN 1087-0571, E-ISSN 1552-454X, Vol. 15, nr 6, s. 671-9Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    15-Lipoxygenase-1 catalyzes the introduction of molecular oxygen into polyunsaturated fatty acids to form a lipid hydroperoxide. The authors have developed an assay for the detection of lipid hydroperoxides formed by human 15-lipoxygenase (15-LO) in enzyme or cellular assays using either a 96-well or a 384-well format. The assays described take advantage of the ability of lipid hydroperoxides to oxidize nonfluorescent diphenyl-1-pyrenylphosphine (DPPP) to a fluorescent phosphine oxide. Oxidation of DPPP yields a fluorescent compound, which is not sensitive to temperature and is stable for more than 2 h. The assay is sensitive toward inhibition and robust with a Z' value of 0.79 and 0.4 in a 96- and 384-well format, respectively, and thus amenable for high-throughput screening. The utility of DPPP as a marker for 15-lipoxygenase activity was demonstrated with both enzyme- and cell-based assays for the identification of hits and to determine potency by IC(50) determinations.

  • 270.
    Dahmani, Younes
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Uttryck av Nfr2 och dess kliniska roll i klarcellig njurcancer2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 271.
    Dakhil, Aseel
    Högskolan Kristianstad, Sektionen för lärande och miljö.
    HLA-typning: Jämförelse mellan mastermix med tillsatt eller inkluderat Ampli Taq DNA polymerase2016Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [en]

    Transplantation is based on a satisfactory matching of the patient and donor genes for Human Leukocyte Antigen (HLA), which increases the chance of a successful transplantation. HLA gives individual cell surface markers. The Major Histocompatibility Complex (MHC) region, encoding HLA in humans, is the most polymorphic in the human genome. The genes are located on chromosome six and consists of 200 genes. Those genes encode protein products essential for the acquired immune system. MHC molecule’s role is to represent foreign substance for B- and T-lymphocytes. MHC is an important system as it contributes to the activation of the immune system to combat viruses, bacteria, parasites and cancer cells. HLA-typing is determined through certain antigens in the HLA system. The classical transplantation antigens are HLA-A, -B, -C, -DR, -DQ and -DP. By amplifying the DNA with sequence specific primers in the Polymerase Chain Reaction (PCR), the amplicons can be detected and alleles present in the patient genome can be determined. The purpose of this study was to compare occurrence of non-specific DNA binding using master mix where Ampli Taq DNA polymerase is added and master mix with polymerase included in the PCR. Samples from 16 patients were tested with both master mix- solutions. The analyses were performed with primer plates for HLA-A, HLA-B and HLA-DRP1. The results showed that the master mix with Taq polymerase included should be applied, because it gave clearer specific band, better image quality and gave weaker and approximately 30% fewer non- specific DNA binding compared to the master mix with added Taq polymerase.

  • 272.
    Dalsätt, Johan
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap. Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
    Utvärdering av olika RNA-preparationsmetoder samt karakterisering av KLK7-uttryck i prostatacancer-relaterade cellinjer och tumörer2015Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 273.
    Dammström, Magdalena
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Användning av Small interfering RNA med syftet att minska uttrycket av insulin like growth factor-1 receptor hos melanomceller2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 274.
    Danielsson, Micaela
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Bindningsmönster av aviärt influensa A-virus till mag-tarmkanalen hos andfåglar2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Influensa A-virus ses som ett stort hot mot människans hälsa med återkommande säsongsutbrott som orsaka upp till 500 000 dödsfall världen över varje år. Virusets naturliga reservoar är vilda vattenfåglar. För att få en bättre förståelse över hur så många olika virus kan infektera vilda fåglar kan virusets bindningsmönster till värden studeras med tekniken virushistokemi. Syftet med detta arbete har varit att undersöka bindningsmönstret av aviärt influensavirus, subtyp H4, H9, H13 och H16, till mag-tarmkanal-vävnader från olika arter av andfåglar för att se om dessa virus kan binda till cellerna och därmed infektera värden. De tekniker som har använts är förökning av viruset genom odling i embryonerade kycklingägg, rening med sukrosgradient, inaktivering med formalin och sedan fluorescein isothiocyanate (FITC)-märkning. Titern av viruset mättes med hemagglutinationsanalys, där visade resultatet att titern efter skörd av ägg för H4 var: 256 HAU/100 µl, H9: 512 HAU/100 µl, H13: 384 HAU/100 µl och för H16: 768 HAU/100 µl. FITC-märkt virustiter mättes till H4: 15 360 HAU/100 µl, H9: 30 720 HAU/100 µl, H13: 163 840 HAU/100 µl och H16: 81 920 HAU/100 µl. Virushistokemin har inte utförts och därför är resultatet över bindningsmönstret fortfarande okänt och frågeställningen inte besvarad. Det som förväntas av resultatet är dock att subtyp H4 och H9, som är vanliga att finna hos andfåglar, kommer att binda till epitelceller i tarmen hos andfåglar medan subtyp H16 och H13, som endast i ett fåtal fall har lyckats isoleras från andfåglar, inte förväntas binda till mag-tarmkanalen hos andfåglar.

  • 275.
    D'Arcy, Padraig
    et al.
    Department of Oncology and Pathology, Karolinska Institute, SE-171 76 Stockholm, Sweden.
    Wang, Xin
    Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för läkemedelsforskning. Linköpings universitet, Hälsouniversitetet. Department of Oncology and Pathology, Karolinska Institute, SE-171 76 Stockholm, Sweden.
    Linder, Stig
    Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för läkemedelsforskning. Linköpings universitet, Hälsouniversitetet. Department of Oncology and Pathology, Karolinska Institute, SE-171 76 Stockholm, Sweden.
    Deubiquitinase inhibition as a cancer therapeutic strategy2015Inngår i: Pharmacology and Therapeutics, ISSN 0163-7258, E-ISSN 1879-016X, Vol. 147, s. 32-54Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    The ubiquitin proteasome system (UPS) is the main system for controlled protein degradation and a key regulator of fundamental cellular processes. The dependency of cancer cells on a functioning UPS has made this an attractive target for development of drugs that show selectivity for tumor cells. Deubiquitinases (DUBs, ubiquitin isopeptidases) are components of the UPS that catalyze the removal of ubiquitin moieties from target proteins or polyubiquitin chains, resulting in altered signaling or changes in protein stability. A number of DUBs regulate processes associated with cell proliferation and apoptosis, and as such represent candidate targets for cancer therapeutics. The majority of DUBs are cysteine proteases and are likely to be more "druggable" than E3 ligases. Cysteine residues in the active sites of DUBs are expected to be reactive to various electrophiles. Various compounds containing α,β-unsaturated ketones have indeed been demonstrated to inhibit cellular DUB activity. Inhibition of proteasomal cysteine DUB enzymes (i.e. USP14 and UCHL5) can be predicted to be particularly cytotoxic to cancer cells as it leads to blocking of proteasome function and accumulation of proteasomal substrates. We here provide an overall review of DUBs relevant to cancer and of various small molecules which have been demonstrated to inhibit DUB activity.

  • 276.
    Darmanis, Spyros
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Nong, Rachel Yuan
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Vänelid, Johan
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Siegbahn, Agneta
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Klinisk kemi.
    Ericsson, Olle
    Halo Genomics AB, Dag Hammarskjölds väg 36B, SE-752 37 Uppsala Sweden.
    Fredriksson, Simon
    Olink Biosciences, Dag Hammarskjölds väg 52B, SE-752 37 Uppsala, Sweden.
    Bäcklin, Christofer
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper.
    Gut, Marta
    Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028 Barcelona, Spain.
    Heath, Simon
    Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028 Barcelona, Spain.
    Gut, Ivo Glynne
    Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028 Barcelona, Spain.
    Wallentin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Uppsala kliniska forskningscentrum (UCR).
    Gustafsson, Mats G.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper.
    Kamali-Moghaddam, Masood
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    Landegren, Ulf
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg. Uppsala universitet, Science for Life Laboratory, SciLifeLab.
    ProteinSeq: high-performance proteomic analyses by proximity ligation and next generation sequencing2011Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 6, nr 9, s. e25583-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Despite intense interest, methods that provide enhanced sensitivity and specificity in parallel measurements of candidate protein biomarkers in numerous samples have been lacking. We present herein a multiplex proximity ligation assay with readout via realtime PCR or DNA sequencing (ProteinSeq). We demonstrate improved sensitivity over conventional sandwich assays for simultaneous analysis of sets of 35 proteins in 5 μl of blood plasma. Importantly, we observe a minimal tendency to increased background with multiplexing, compared to a sandwich assay, suggesting that higher levels of multiplexing are possible. We used ProteinSeq to analyze proteins in plasma samples from cardiovascular disease (CVD) patient cohorts and matched controls. Three proteins, namely P-selectin, Cystatin-B and Kallikrein-6, were identified as putative diagnostic biomarkers for CVD. The latter two have not been previously reported in the literature and their potential roles must be validated in larger patient cohorts. We conclude that ProteinSeq is promising for screening large numbers of proteins and samples while the technology can provide a much-needed platform for validation of diagnostic markers in biobank samples and in clinical use. 

  • 277. Darsalia, Vladimer
    et al.
    Mansouri, Shiva
    Ortsater, Henrik
    Olverling, Anna
    Nozadze, Nino
    Kappe, Camilla
    Iverfeldt, Kerstin
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.
    Tracy, Linda M.
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.
    Grankvist, Nina
    Sjöholm, Åke
    Patrone, Cesare
    Glucagon-like peptide-1 receptor activation reduces ischaemic brain damage following stroke in Type 2 diabetic rats2012Inngår i: Clinical Science, ISSN 0143-5221, E-ISSN 1470-8736, Vol. 122, nr 9-10, s. 473-483Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Diabetes is a strong risk factor for premature and severe stroke. The GLP-IR (glucagon-like peptide-1 receptor) agonist Ex-4 (exendin-4) is a drug for the treatment of T2D (Type 2 diabetes) that may also have neuroprotective effects. The aim of the present study was to determine the efficacy of Ex-4 against stroke in diabetes by using a diabetic animal model, a drug administration paradigm and a dose that mimics a diabetic patient on Ex-4 therapy. Furthermore, we investigated inflammation and neurogenesis as potential cellular mechanisms underlying the Ex-4 efficacy. A total of seven 9-month-old Type 2 diabetic Goto-Kakizaki rats were treated peripherally for 4 weeks with Ex-4 at 0.1, 1 or 5 mu g/kg of body weight before inducing stroke by transient middle cerebral artery occlusion and for 2-4 weeks thereafter. The severity of ischaemic damage was measured by evaluation of stroke volume and by stereological counting of neurons in the striatum and cortex. We also quantitatively evaluated stroke-induced inflammation, stem cell proliferation and neurogenesis. We show a profound anti-stroke efficacy of the clinical dose of Ex-4 in diabetic rats, an arrested microglia infiltration and an increase of stroke-induced neural stem cell proliferation and neuroblast formation, while stroke-induced neurogenesis was not affected by Ex-4. The results show a pronounced anti-stroke, neuroprotective and anti-inflammatory effect of peripheral and chronic Ex-4 treatment in middle-aged diabetic animals in a preclinical setting that has the potential to mimic the clinical treatment. Our results should provide strong impetus to further investigate GLP-IR agonists for their neuroprotective action in diabetes, and for their possible use as anti-stroke medication in non-diabetic conditions.

  • 278.
    Davidsson, Hans
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Syntes och kvalitetskontroll av [18F]FDG på TRACERlab MX2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 279.
    Dawod, Salima
    et al.
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för naturvetenskap och biomedicin.
    Eliassi, Lana
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för naturvetenskap och biomedicin.
    En jämförelse mellan auskultatoriska och oscillometriska blodtrycksvärden i vila och efter ansträngning.2019Independent thesis Basic level (degree of Bachelor), 180 hpOppgave
    Abstract [sv]

    Bakgrund: I hälso-och sjukvården är blodtrycksmätning en viktig och grundläggande metod vid korrekt diagnostik och hantering av högt blodtryck. Forskning har under flera år påvisat att olika komponenter påverkar noggrannheten av blodtrycksmätningen. Eftersom auskultatorisk och oscillometrisk blodtrycksmätning utförs på två olika sätt finns en risk att blodtrycksvärdet kan variera mellan metoderna. Syfte: Syftet med studien var att undersöka om det finns någon skillnad mellan auskultatorisk och oscillometrisk blodtrycksmätning utfört i både vila och efter ansträngning. Material och metod: Studien bestod av 20 slumpmässigt utvalda studenter från Hälsohögskolan i Jönköping. Blodtrycksmätning utfördes med hjälp av auskultatorisk blodtrycksmanschett med handmanometer och stetoskop samt oscillometrisk modalitet (OMRON M7). Resultat: Statistisk signifikant skillnad observerades mellan auskultatorisk och oscillometrisk modalitet, både i vila och efter ansträngning. Skillnaden är som störst efter ansträngning för auskultatorisk och oscillometrisk mätmetod, i både systoliskt och diastoliskt blodtrycksvärde. Diskussion: I vården har användning av oscillometrisk blodtrycksmodalitet ökat och därmed finns en risk för minskad reliabilitet och validitet av blodtrycksvärdet. Slutsatser: Statistisk signifikant skillnad föreligger mellan modaliteterna, både i vila och efter ansträngning.

  • 280.
    de Gelidi, S.
    et al.
    Middlesex Univ, UK.
    Seifnaraghi, N.
    Middlesex Univ, UK.
    Bardill, A.
    Middlesex Univ, UK.
    Tizzard, A.
    Middlesex Univ, UK.
    Wu, Y.
    UCL, UK.
    Sorantin, E.
    Med Univ Graz, Austria..
    Nordebo, Sven
    Linnéuniversitetet, Fakulteten för teknik (FTK), Institutionen för fysik och elektroteknik (IFE).
    Demosthenous, A.
    UCL, UK.
    Bayford, R.
    Middlesex Univ, UK.
    Torso shape detection to improve lung monitoring2018Inngår i: Physiological Measurement, ISSN 0967-3334, E-ISSN 1361-6579, Vol. 39, nr 7, artikkel-id 074001Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective: Newborns with lung immaturity often require continuous monitoring and treatment of their lung ventilation in intensive care units, especially if born preterm. Recent studies indicate that electrical impedance tomography (EIT) is feasible in newborn infants and children, and can quantitatively identify changes in regional lung aeration and ventilation following alterations to respiratory conditions. Information on the patient-specific shape of the torso and its role in minimizing the artefacts in the reconstructed images can improve the accuracy of the clinical parameters obtained from EIT. Currently, only idealized models or those segmented from CT scans are usually adopted. Approach: This study presents and compares two methodologies that can detect the patient-specific torso shape by means of wearable devices based on (1) previously reported bend sensor technology, and (2) a novel approach based on the use of accelerometers. Main results: The reconstruction of different phantoms, taking into account anatomical asymmetries and different sizes, are produced for comparison. Significance: As a result, the accelerometers are more versatile than bend sensors, which cannot be used on bigger cross-sections. The computational study estimates the optimal number of accelerometers required in order to generate an image reconstruction comparable to the use of a CT scan as the forward model. Furthermore, since the patient position is crucial to monitoring lung ventilation, the orientation of the phantoms is automatically detected by the accelerometer-based method.

  • 281.
    Delbro, Dick
    Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap. Sahlgrens Univ Hosp, Dept Surg, Gothenburg, Sweden..
    Do neuro-humoral signaling molecules participate in colorectal carcinogenesis/cancer progression?2012Inngår i: Neurogastroenterology and Motility, ISSN 1350-1925, E-ISSN 1365-2982, Vol. 24, nr 2, s. 96-99Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Signaling molecules in the gastrointestinal (GI) tract, as released from intrinsic, or extrinsic neurons, or from local endocrine cells may serve as positive or negative growth factors, and it has been suggested that such could participate also in colorectal carcinogenesis/cancer progression. Sporadic colorectal cancer arises from an initially benign adenoma, which, in turn, develops from the stem cell compartment, located in the bottom of the crypts of the colorectal mucosa. It was recently demonstrated in rat that intrinsic denervation of the colon appeared to be protective against chemically induced carcinogenesis. Of the various GI signaling molecules, noradrenaline (NA) and substance P (SP) may be of particular importance as growth factors involved in colorectal cancer. In the current issue of Neurogastroenterology and Motility, Graf et al. demonstrate that in benign, human colon polyps, there was a loss of innervation compared with adjacent mucosa, affecting efferent, noradrenergic, as well as sensory, SP-ergic fibers, while there was an increase in SP-immunoreactive non-neuronal cells in the polyps. The results obtained could suggest that loss of mucosal innervation, due to e.g. luminal, pro-inflammatory stimuli, could result in unbalanced pro-tumorigenic stimulation of the stem cell region by non-neuronal SP. The current findings may be important for the further understanding of the development of sporadic colorectal cancer.

  • 282.
    Delbro, Dick S.
    Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
    Expression of the non-neuronal cholinergic system in rat beta-cells2012Inngår i: Autonomic Neuroscience: Basic & Clinical, ISSN 1566-0702, E-ISSN 1872-7484, Vol. 167, nr 1-2, s. 75-77Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Various markers of the cholinergic system (like e.g. choline acetyltransferase) were demonstrated by immunohistochemistry in, seemingly, beta-cells of rat pancreas. The findings may suggest an autocrine role of acetylcholine for the beta-cells. (C) 2011 Elsevier B.V. All rights reserved.

  • 283. Desmarais, Samantha M
    et al.
    Tropini, Carolina
    Miguel, Amanda
    Cava, Felipe
    Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
    Monds, Russell D
    de Pedro, Miguel A
    Huang, Kerwyn Casey
    High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography2015Inngår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 290, nr 52, s. 31090-31100Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The bacterial cell wall is a network of glycan strands crosslinked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination. Liquid chromatography can be used to measure the abundance of the muropeptide subunits composing the cell wall. Characteristics such as the degree of cross-linking and average glycan strand length are known to vary across species. However, a systematic comparison among strains of a given species has yet to be undertaken, making it difficult to assess the origins of variability in peptidoglycan composition. We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time. We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software. This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences. Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis. High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.

  • 284.
    Desso, Dawit Dejene
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Jämförelse av Bruker Sepsityper-kit med in-house extraktionsmetoder för direkt bakterieidentifiering i positiva blododlingar2018Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 285.
    Diab, Zeina
    Örebro universitet, Hälsoakademin.
    Referensvärdesbestämning av fraktionerad sensorisk nervus medianusmätning över karpaltunneln2011Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 286.
    Dijkstra, Erik J.
    et al.
    KTH, Skolan för teknikvetenskap (SCI), Mekanik, Biomekanik.
    Gutierrez-Farewik, Elena M.
    KTH, Skolan för teknikvetenskap (SCI), Mekanik, Biomekanik. Karolinska Institutet, Stockholm, Sweden.
    Computation of ground reaction force using Zero Moment Point2015Inngår i: Journal of Biomechanics, ISSN 0021-9290, E-ISSN 1873-2380, Vol. 48, nr 14, s. 3776-3781Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Motion analysis is a common clinical assessment and research tool that uses a camera system or motion sensors and force plates to collect kinematic and kinetic information of a subject performing an activity of interest. The use of force plates can be challenging and sometimes even impossible. Over the past decade, several computational methods have been developed that aim to preclude the use of force plates. Useful in particular for predictive simulations, where a new motion or change in control strategy inherently means different external contact loads. These methods, however, often depend on prior knowledge of common observed ground reaction force (GRF) patterns, are computationally expensive, or difficult to implement. In this study, we evaluated the use of the Zero Moment Point as a computationally inexpensive tool to obtain the GRFs for normal human gait. The method was applied on ten healthy subjects walking in a motion analysis laboratory and predicted GRFs are evaluated against the simultaneously measured force plate data. Apart from the antero-posterior forces, GRFs are well-predicted and errors fall within the error ranges from other published methods. Joint extension moments were underestimated at the ankle and hip but overestimated at the knee, attributable to the observed discrepancy in the predicted application points of the GRFs. The computationally inexpensive method evaluated in this study can reasonably well predict the GRFs for normal human gait without using prior knowledge of common gait kinetics.

  • 287.
    Dimberg, Jan
    et al.
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för naturvetenskap och biomedicin. Högskolan i Jönköping, Hälsohögskolan, HHJ. Biomedicinsk plattform.
    Skarstedt, Marita
    Department of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Löfgren, Sture
    Department of Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
    Zar, Niklas
    Department of Surgery, Ryhov County Hospital, Jönköping, Sweden.
    Matussek, Andreas
    Department of Laboratory Services, Ryhov County Hospital, Jönköping, Sweden.
    Protein expression and gene polymorphism of CXCL10 in patients with colorectal cancer2014Inngår i: Biomedical Reports, ISSN 2049-9442, Vol. 2, nr 3, s. 340-343Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Chemokines (chemotactic cytokines) promote leukocyte attraction to sites of inflammation and cancer. Certain chemokines promote and regulate neoplastic progression, including metastasis and angiogenesis. One such chemokine, CXCL10, was found to be expressed in colorectal cancer (CRC) tissue. To gain insight into the prognostic significance of CXCL10, we investigated whether the levels of this chemokine were altered in the colorectal tissue or plasma of CRC patients. Using Luminex technology for protein analyses, we observed a significantly higher CXCL10 protein level in cancer tissue compared to that in paired normal tissue. Moreover, significantly higher plasma levels of CXCL10 were detected in patients compared to those in control subjects and the plasma levels of CXCL10 in disseminated disease were found to be significantly higher compared to those in localized disease. The single‑nucleotide polymorphism rs8878, which has been described in exon 4 in the 3'‑untranslated region of the CXCL10 gene, was investigated using a TaqMan system. There were significant differences in genotype distribution and allelic frequencies between CRC patients and control subjects. In conclusion, altered CXCL10 protein concentrations in CRC tissues or plasma and the rs8878 genotype variant of CXCL10 may contribute to the prediction of clinical outcome.

  • 288.
    Dobos, Rebecca
    Örebro universitet, Institutionen för hälsovetenskaper.
    Extremitetelektrodernas inverkan på QRS-amplituden och den elektriska axeln i ett elektrokardiogram2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 289. Dombovari, Balazs
    et al.
    Fiath, Richard
    Kerekes, Balint Peter
    Toth, Emilia
    Wittner, Lucia
    Horvath, Domonkos
    Seidl, Karsten
    Herwik, Stanislav
    Torfs, Tom
    Paul, Oliver
    Ruther, Patrick
    Neves, Hercules Pereira
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik.
    Ulbert, Istvan
    In vivo validation of the electronic depth control probes2014Inngår i: Biomedizinische Technik (Berlin. Zeitschrift), ISSN 1862-278X, E-ISSN 0013-5585, Vol. 59, nr 4, s. 283-289Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this article, we evaluated the electrophysiological performance of a novel, high-complexity silicon probe array. This brain-implantable probe implements a dynamically reconfigurable voltage-recording device, coordinating large numbers of electronically switchable recording sites, referred to as electronic depth control (EDC). Our results show the potential of the EDC devices to record good-quality local field potentials, and single- and multiple-unit activities in cortical regions during pharmacologically induced cortical slow wave activity in an animal model.

  • 290.
    Donà, Valentina
    et al.
    Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
    Low, Nicola
    Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland.
    Golparian, Daniel
    Örebro universitet, Institutionen för medicinska vetenskaper. WHO Collaborating Centre for Gonorrhoea, Örebro University Hospital, Örebro, Sweden.
    Unemo, Magnus
    WHO Collaborating Centre for Gonorrhoea, Örebro University Hospital, Örebro, Sweden.
    Recent advances in the development and use of molecular tests to predict antimicrobial resistance in Neisseria gonorrhoeae2017Inngår i: Expert Review of Molecular Diagnostics, ISSN 1473-7159, E-ISSN 1744-8352, Vol. 17, nr 9, s. 845-859Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Introduction: The number of genetic tests, mostly real-time PCRs, to detect antimicrobial resistance (AMR) determinants and predict AMR in Neisseria gonorrhoeae is increasing. Several of these assays are promising, but there are important shortcomings and few assays have been adequately validated and quality assured.

    Areas covered: Recent advances, focusing on publications since 2012, in the development and use of molecular tests to predict gonococcal AMR for surveillance and for clinical use, advantages and disadvantages of these tests and of molecular AMR prediction compared with phenotypic AMR testing, and future perspectives for effective use of molecular AMR tests for different purposes.

    Expert commentary: Several challenges for direct testing of clinical, especially extra-genital, specimens remain. The choice of molecular assay needs to consider the assay target, quality controls, sample types, limitations intrinsic to molecular technologies, and specific to the chosen methodology, and the intended use of the test. Improved molecular- and particularly genome-sequencing-based methods will supplement AMR testing for surveillance purposes, and translate into point-of-care tests that will lead to personalized treatments, while sparing the last available empiric treatment option (ceftriaxone). However, genetic AMR prediction will never completely replace phenotypic AMR testing, which detects also AMR due to unknown AMR determinants.

  • 291. Downs, J.
    et al.
    Velupillai, Sumithra
    KTH, Skolan för elektroteknik och datavetenskap (EECS), Teoretisk datalogi, TCS.
    George, G.
    Holden, R.
    Kikoler, M.
    Dean, H.
    Fernandes, A.
    Dutta, R.
    Detection of Suicidality in Adolescents with Autism Spectrum Disorders: Developing a Natural Language Processing Approach for Use in Electronic Health Records2017Inngår i: Advances in Printing and Media Technology, ISSN 0892-2284, E-ISSN 1942-597X, Vol. 2017, s. 641-649Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Over 15% of young people with autism spectrum disorders (ASD) will contemplate or attempt suicide during adolescence. Yet, there is limited evidence concerning risk factors for suicidality in childhood ASD. Electronic health records (EHRs) can be used to create retrospective clinical cohort data for large samples of children with ASD. However systems to accurately extract suicidality-related concepts need to be developed so that putative models of suicide risk in ASD can be explored. We present a systematic approach to 1) adapt Natural Language Processing (NLP) solutions to screen with high sensitivity for reference to suicidal constructs in a large clinical ASD EHR corpus (230,465 documents), and 2) evaluate within a screened subset of 500 patients, the performance of an NLP classification tool for positive and negated suicidal mentions within clinical text. When evaluated, the NLP classification tool showed high system performance for positive suicidality with precision, recall, and F1 scores all > 0.85 at a document and patient level. The application therefore provides accurate output for epidemiological research into the factors contributing to the onset and recurrence of suicidality, and potential utility within clinical settings as an automated surveillance or risk prediction tool for specialist ASD services.

  • 292.
    Doyo, Kader
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
    A prospective randomized study to compare Nidoil and Ovoil cultur oils used to culture human embryos in IVF therapy2016Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
    Abstract [en]

    Background: Since the initiation of assisted reproduction techniques, several studies has been performed to improve treatment results by development of culture conditions like embryo oil and culture media used. In this study, two embryonic oils from different companies, Nidoil and Ovoil were examined.Method: In this study, 47 human embryos were used. All embryos were donated for research purposes by couples who had been treated at the clinic in Uppsala University Hospital. The embryos were divided into two groups, one group was cultured with Ovoil and the other with Nidoil.Results: There was no difference between the two oils, the embryo quality was the same in both groups.CONCLUSION: The result was expected because both oils had the same composition and purity.

  • 293. Draeger, A
    et al.
    Nathrath, WBJ
    Lane, EB
    Sundström, Birgitta
    Karlstads universitet, Fakulteten för teknik- och naturvetenskap, Avdelningen för kemi och biomedicinsk vetenskap.
    Stigbrand, T
    Immunohistochemical localization of cytokeratins, smooth muscle actin and vimentin in human normal salivery glands and pleomorphic adenomas with particular reference to myoepithelial and basal cells1991Inngår i: APMIS 1991;99:405-415Artikkel i tidsskrift (Fagfellevurdert)
  • 294.
    Dragan, Smiljic
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Studies of small bicoid knock-down and overexpression at early and late stage of development in Drosophila melanogaster.2016Independent thesis Advanced level (degree of Master (One Year)), 20 poäng / 30 hpOppgave
  • 295. Dresch, Erica
    GENETIC CHARACTERIZATION AND DISINFECTANT SUSCEPTIBILITY TESTING OF VEROTOXIN-PRODUCING Escherichia coli O157:H7 FROM SWEDISH CATTLE2015Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
    Abstract [en]

    Background Verotoxin-producing Escherichia coli (VTEC) O157:H7 is a common cause of intestinal infections in humans. Cattle is the main reservoir, and food or water contaminated by animal feces are common sources of infection. The symptoms are usually bloody diarrhea and abdominal pain, and complications as haemolytic uremic syndrome (HUS) may occur. Chlorhexidine is a widely used disinfectant both in veterinary and human medicine. Treatments using chlorhexidine have shown to be effective in reducing the fecal shedding of VTEC O157:H7.Aim The aim of this study was to genetically characterize and type a selection of VTEC O157:H7 isolates found in Swedish cattle. Additionally, the purpose was to determine if four major subgroups of VTEC O157:H7 show different levels of susceptibility against chlorhexidine.Material and methods The E. coli strains analysed were isolated from fecal samples of cattle. The characterization method applied was next generation sequencing, and the susceptibility test was performed using the broth macrodilution method.Results The results showed that the prevalence of the most virulent subgroup has decreased compared to past years. The susceptibility test showed no significant difference between the four subgroups.Conclusion The results obtained in this study have improved the knowledge about the strains currently present in the country, which facilitates the monitoring and control processes. The data obtained shows that the prevalence of the most pathogenic subgroup has decreased. The major subgroups of VTEC O157:H7 have similar levels of susceptibility against chlorhexidine, which is useful information as chlorhexidine is a widely used disinfectant.

  • 296.
    Dulaimi, Omar Ali Hussein
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Ingen association mellan polymorfismen rs7169289 i genen för retinalaldehydrogenas ALDH1A2 och hjärtinfarkt2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 297.
    Dybeck, Josefin
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
    Expression of otic developmental markers in the adult human vestibular system2016Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
    Abstract [en]

    The sense of hearing and balance depend on the highly organized arrangement of sensory hair cells and non-sensory supporting cells in the cochlea and in the balance organs of the vestibular system. Hair cell-dependent hearing loss is permanent due to the inability of the hair cells to regenerate. Recent research has determined that differentiation of supporting cells into hair cells could be induced by forcing expression of certain otic developmental genes. The cochlea would be the ideal model to study, however, because of the limited accessibility, the balance organs have become the second choice because of the similarities to the cochlea. This project offered a unique opportunity of access to surgical waste consisting of tissue from the vestibular system.The purpose was to stain the tissue for different developmental markers in order to find cells that might have the capacity of differentiating into new hair cells. The tissue was therefore stained for different markers associated with otic development and the expression was visualized by immunofluorescence.We found expression of the developmental marker Pax-2 in the balance organs, as well as in the endolymphatic sac. Expression of the proliferation marker Ki-67 was found in the endolymphatic sac but not in the balance organs, suggesting that the different organs have extensively diverse functions, although equally important in maintaining the inner ear homeostasis.These results offer a basis for further research in the pursuit of developing a potential gene therapy-based treatment to cure hair cell-dependent hearing loss.

  • 298.
    Dyverfeldt, Petter
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för ekonomisk och industriell utveckling, Mekanisk värmeteori och strömningslära. Linköpings universitet, Tekniska högskolan.
    Extending MRI to the Quantification of Turbulence Intensity2010Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    In cardiovascular medicine, the assessment of blood flow is fundamental to the understanding and detection of disease. Many pharmaceutical, interventional, and surgical treatments impact the flow. The primary purpose of the cardiovascular system is to drive, control and maintain blood flow to all parts of the body. In the normal cardiovascular system, fluid transport is maintained at high efficiency and the blood flow is essentially laminar. Disturbed and turbulent blood flow, on the other hand, appears to be present in many cardiovascular diseases and may contribute to their initiation and progression. Despite strong indications of an important interrelationship between flow and cardiovascular disease, medical imaging has lacked a non-invasive tool for the in vivo assessment of disturbed and turbulent flow. As a result, the extent and role of turbulence in the blood flow of humans have not yet been fully investigated.

    Magnetic resonance imaging (MRI) is a versatile tool for the non-invasive assessment of flow and has several important clinical and research applications, but might not yet have reached its full potential. Conventional MRI techniques for the assessment of flow are based on measurements of the mean velocity within an image voxel. The mean velocity corresponds to the first raw moment of the distribution of velocities within a voxel. An MRI framework for the quantification of any moment (mean, standard deviation, skew, etc.) of arbitrary velocity distributions is presented in this thesis.

    Disturbed and turbulent flows are characterized by velocity fluctuations that are superimposed on the mean velocity. The intensity of these velocity fluctuations can be quantified by their standard deviation, which is a commonly used measure of turbulence intensity. This thesis focuses on the development of a novel MRI method for the quantification of turbulence intensity. This method is mathematically derived and experimentally validated. Limitations and sources of error are investigated and guidelines for adequate application of MRI measurements of turbulence intensity are outlined. Furthermore, the method is adapted to the quantification of turbulence intensity in the pulsatile blood flow of humans and applied to a wide range of cardiovascular diseases. In these applications, elevated turbulence intensity was consistently detected in regions where highly disturbed flow was anticipated, and the effects of potential sources of errors were small.

    Diseased heart valves are often replaced with prosthetic heart valves, which, in spite of improved benefits and durability, continue to fall short of matching native flow patterns. In an in vitro setting, MRI was used to visualize and quantify turbulence intensity in the flow downstream from four common designs of prosthetic heart valves. Marked differences in the extent and degree of turbulence intensity were detected between the different valves.

    Mitral valve regurgitation is a common valve lesion associated with progressive left atrial and left ventricular remodelling, which may often require surgical correction to avoid irreversible ventricular dysfunction. The spatiotemporal dynamics of flow disturbances in mitral regurgitation were assessed based on measurements of flow patterns and turbulence intensity in a group of patients with significant regurgitation arising from similar valve lesions. Peak turbulence intensity occurred at the same time in all patients and the total turbulence intensity in the left atrium appeared closely related to the severity of regurgitation.

    MRI quantification of turbulence intensity has the potential to become a valuable tool in investigating the extent, timing and role of disturbed blood flow in the human cardiovascular system, as well as in the assessment of the effects of different therapeutic options in patients with vascular or valvular disorders.

  • 299.
    Dyverfeldt, Petter
    et al.
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Escobar Kvitting, John Peder
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicin och hälsa, Fysiologi. Östergötlands Läns Landsting, Hjärtcentrum, Thorax-kärlkliniken.
    Boano, G.
    Östergötlands Läns Landsting.
    Carlhäll, Carljohan
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Sigfridsson, Andreas
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Hermansson, Ulf
    Linköpings universitet, Institutionen för medicin och hälsa, Thoraxkirurgi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärtcentrum, Thorax-kärlkliniken.
    Bolger, A.F.
    University of California, San Fransisco, San Franisco, California, United States.
    Engvall, Jan
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Ebbers, Tino
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Turbulence Mapping Extends the Utility of Phase-Contrast MRI in Mitral Valve Regurgitation2009Inngår i: Proc. Intl. Soc. Mag. Reson. Med., 2009, s. 3939-Konferansepaper (Fagfellevurdert)
  • 300.
    Dyverfeldt, Petter
    et al.
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Escobar Kvitting, John-Peder
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Fysiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärtcentrum, Thorax-kärlkliniken.
    Sigfridsson, Andreas
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    Franzén, Stefan
    Linköpings universitet, Institutionen för medicin och hälsa, Thoraxkirurgi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärtcentrum, Thorax-kärlkliniken.
    Bolger, Ann F.
    University of California San Fransisco, San Fransisco, California, United States.
    Ebbers, Tino
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicin och hälsa, Klinisk fysiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärtcentrum, Fysiologiska kliniken.
    In-Vitro Turbulence Mapping in Prosthetic Heart Valves using Generalized Phase-Contrast MRI2009Inngår i: Proc. Intl. Soc. Mag. Reson. Med., 2009, s. 3941-Konferansepaper (Fagfellevurdert)
3456789 251 - 300 of 1389
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf