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  • 1201.
    Walther, Emelie
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap. Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Serumresistens och biofilmbildning hos Aggregatibacter aphrophilus: Betydelsen av H-NS2018Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1202.
    Wang, Li
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap. Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
    Uttryck och lokalisering av TβRI ICD i endometrioid carcinom cellinje HEC-1B och RL 95-2: Undersöka om det fanns tydlig koppling mellan nukleär TβRI-ICD och progressionen av endometrioid carcinom2018Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1203.
    Wang, Sen
    et al.
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Fan, Zheng
    Office of Teaching Affairs, Xi'an University, Xi'an, China.
    Zhou, Bing
    Key Laboratory of Hormones and Development (Ministry of Health), Key laboratory of metabolic disease (Tianjin), Metabolic Diseases Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.
    Wang, Yingting
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Du, Peiru
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Tan, Wuhong
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Lammi, Mikko
    Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB). School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Guo, Xiong
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Roles of glycoproteins in the diagnosis and differential diagnosis of chronic and latent Keshan disease2017Inngår i: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 22, nr 5, artikkel-id 746Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We aimed to explore the roles of glycoproteins in the pathogenesis of chronic and latent Keshan disease (CKD and LKD), and screen the lectins as indicators of significant differences in glycoproteins of KD saliva and serum. Blood and saliva were collected from 50 CKD, 50 LKD patients and 54 normal individuals. Saliva and serum lectin microarrays and saliva and serum microarrays were used to screen and verify the differences in the levels of lectin among the three groups. In the male saliva lectin microarray, Solanum tuberosum (potato) lectin (STL) and other 9 lectins showed differences between CKD and normal; STL and other 9 lectins showed differences between LKD and normal; Aleuria aurantia lectin (AAL) and other 15 lectins showed differences between CKD and LKD. In the female saliva microarray, Griffonia (Bandeiraea) simplicifolia lectin I (GSL-I) and other 9 lectins showed differences between CKD and normal; STL and other 7 lectins showed differences between LKD and normal; Maackia amurensis lectin I (MAL-I) and Triticum vulgaris (WGA) showed difference between CKD and LKD. In the male serum lectin microarray, Psophocarpus tetragonolobus lectin I (PTL-I) and other 16 lectins showed differences between CKD and normal; Ulexeuropaeus agglutinin I (UEA-I) and other 9 lectins showed differences between LKD and normal; AAL and other 13 lectins showed differences between CKD and LKD. In the female serum lectin microarray, WGA and other 13 lectins showed differences between CKD and normal; Euonymus europaeus lectin (EEL) and other 6 lectins showed differences between LKD and normal; MAL-I and other 14 lectins showed differences between CKD and LKD. Carbohydrate chain GlcNAc and α-Gal may play crucial roles in the pathogenesis of KD. STL may be considered the diagnostic biomarker for male CKD and LKD, while WGA may be useful in distinguishing between the two stages. STL may be considered the diagnostic biomarker for female LKD.

  • 1204.
    Wang, Sen
    et al.
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China; Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi, China.
    Zhao, Guanghui
    Xi'an Honghui Hospital, Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
    Shao, Wanzhen
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China; Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi, China.
    Liu, Huan
    School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China; Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi, China.
    Wang, Weizhuo
    Orthopedic Department, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
    Wu, Cuiyan
    Orthopedic Department, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
    Lammi, Mikko
    Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB). Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi, China; School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    Guo, Xiong
    Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi, China; School of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an, China.
    The importance of Se-related genes in the chondrocyte of Kashin-Beck disease revealed by whole genomic microarray and network analysis2019Inngår i: Biological Trace Element Research, ISSN 0163-4984, E-ISSN 1559-0720, Vol. 187, nr 2, s. 367-375Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Kashin-Beck disease (KBD) is an endemic, chronic, and degenerative osteoarthropathy. Selenium (Se) deficiency plays important role in the pathogenesis of KBD. We aimed to screen Se-related gene from chondrocytes of patients with KBD. Whole-genome oligonucleotide microarrays were used to detect differentially expressed genes. qRT-PCR was used to confirm the microarray results. Comparative Toxicogenomics Database (CTD) was used to screen Se-related genes from differentially expressed genes. Gene Ontology (GO) classifications and network analysis of Se-related genes were constituted by STRING online system. Three hundred ninety-nine differentially expressed genes were obtained from microarray. Among them, 54 Se-related genes were identified by CTD. The qRT-PCR validation showed that four genes expressed similarly with the ones in the microarray transcriptional profiles. The Se-related genes were categorized into 6 cellular components, 8 molecular functions, 44 biological processes, 10 pathways, and 1 network by STRING. The Se-related gene insulin-like growth factor binding protein 2 (IGFBP2), insulin-like growth factor binding protein 3 (IGFBP3), interleukin 6 (IL6), BCL2, apoptosis regulator (BCL2), and BCL2-associated X, apoptosis regulator (BAX), which involved in many molecular functions, biological processes, and apoptosis pathway may play important roles in the pathogenesis of KBD.

  • 1205.
    Wang, Yang
    et al.
    Vrije Univ Amsterdam Med Ctr, Netherlands; Amsterdam Publ Hlth Res Inst, Netherlands; Oticon AS, Denmark.
    Zekveld, Adriana
    Linköpings universitet, Institutet för handikappvetenskap (IHV). Linköpings universitet, Institutionen för beteendevetenskap och lärande, Handikappvetenskap. Linköpings universitet, Filosofiska fakulteten. Vrije Univ Amsterdam Med Ctr, Netherlands; Amsterdam Publ Hlth Res Inst, Netherlands.
    Wendt, Dorothea
    Oticon AS, Denmark; Tech Univ Denmark, Denmark.
    Lunner, Thomas
    Linköpings universitet, Institutionen för beteendevetenskap och lärande, Handikappvetenskap. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Institutet för handikappvetenskap (IHV). Oticon AS, Denmark; Tech Univ Denmark, Denmark.
    Naylor, Graham
    MRC, Scotland.
    Kramer, Sophia E.
    Vrije Univ Amsterdam Med Ctr, Netherlands; Amsterdam Publ Hlth Res Inst, Netherlands.
    Pupil light reflex evoked by light-emitting diode and computer screen: Methodology and association with need for recovery in daily life2018Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, nr 6, artikkel-id e0197739Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objectives Pupil light reflex (PLR) has been widely used as a method for evaluating parasympathetic activity. The first aim of the present study is to develop a PLR measurement using a computer screen set-up and compare its results with the PLR generated by a more conventional setup using light-emitting diode (LED). The parasympathetic nervous system, which is known to control the rest and digest response of the human body, is considered to be associated with daily life fatigue. However, only few studies have attempted to test the relationship between self-reported daily fatigue and physiological measurement of the parasympathetic nervous system. Therefore, the second aim of this study was to investigate the relationship between daily-life fatigue, assessed using the Need for Recovery scale, and parasympathetic activity, as indicated by the PLR parameters. Design A pilot study was conducted first to develop a PLR measurement set-up using a computer screen. PLRs evoked by light stimuli with different characteristics were recorded to confirm the influence of light intensity, flash duration, and color on the PLRs evoked by the system. In the subsequent experimental study, we recorded the PLR of 25 adult participants to light flashes generated by the screen set-up as well as by a conventional LED set-up. PLR parameters relating to parasympathetic and sympathetic activity were calculated from the pupil responses. We tested the split-half reliability across two consecutive blocks of trials, and the relationships between the parameters of PLRs evoked by the two set-ups. Participants rated their need for recovery prior to the PLR recordings. Results PLR parameters acquired in the screen and LED set-ups showed good reliability for amplitude related parameters. The PLRs evoked by both set-ups were consistent, but showed systematic differences in absolute values of all parameters. Additionally, higher need for recovery was associated with faster and larger constriction of the PLR. Conclusions This study assessed the PLR generated by a computer screen and the PLR generated by a LED. The good reliability within set-ups and the consistency between the PLRs evoked by the set-ups indicate that both systems provides a valid way to evoke the PLR. A higher need for recovery was associated with faster and larger constricting PLRs, suggesting increased levels of parasympathetic nervous system activity in people experiencing higher levels of need for recovery on a daily basis.

  • 1206.
    Wass, Sofie
    et al.
    Jonkoping Univ, Sweden.
    Vimarlund, Vivian
    Linköpings universitet, Institutionen för datavetenskap, Interaktiva och kognitiva system. Linköpings universitet, Tekniska fakulteten. Jonkoping Univ, Sweden.
    The Role of PAEHRs in Patient Involvement2018Inngår i: Journal of medical systems, ISSN 0148-5598, E-ISSN 1573-689X, Vol. 42, nr 11, artikkel-id 210Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    With increased patient access to data, healthcare services are experiencing change where patients are moving away from being mere passive actors towards becoming more active and involved participants. In this paper, we explore the role of patient accessible electronic health records (PAEHRs) with respect to this increase in patient involvement. The study was performed as a case study and included nine interviews with patients and a survey that was responded to by 56 patients. Our results show that PAEHRs have a role in the enhancement of patient involvement because PAEHRs (i) foster a more balanced relationship between patients and healthcare professionals and (ii) increase access to information.

  • 1207.
    Weglin, Susanne
    Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för hälsovetenskaper.
    Jämförelse mellan C-reaktivt protein,IL-1β och IL-4 nivåer efter träninghos individer med artros2017Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1208.
    Weibull, Emilie
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi.
    Miniaturised Microwell-based Cell Assays2014Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Cell heterogeneity in genetically identical cell populations is becoming a well-known and important phenomenon in cell biology. Current methods commonly utilise population-based analysis founded on averaged result. Hence there is a need for high-throughput cell assays on the single-cell level. By using miniaturised devices it is possible to enhance spatial and temporal control of the individual cells, increase the potential throughput and minimise the needed sample and reagent volume while enabling a wide range of biological applications.

    This thesis is based on the results generated with a miniaturised microwell slide for cell assays. The microwell slide’s high-throughput compartmentalised configuration enables several hundred isolated experiments to be run simultaneously. The bottom of the wells is made out of a thin glass slide, which supports high-resolution imaging. The slide has a standardised format and its’ compatibility with conventional instruments is used extensively throughout the thesis. The presented papers all contribute to the development of the microwell slide by adding technical value or increasing the number of potential applications. For example, the slide was success-fully implemented as a chip-to-world output format for single microfluidic droplets in Paper I, by interfacing two miniaturised systems with fluorescence-activated cell sorting. In Paper II and III, microfluidic channels were integrated to increase spatial and temporal control of the added samples and reagents. In Paper II an automated stepwise concentration gradient generator was developed delivering a drug gradient to adherent mammalian cells in designated wells. In Paper III fluidic-imposed shear stress on endothelial cells was studied. In Paper IV, the slide was functionalised by coating the surfaces of the wells with several antibiotics at a defined concentration range. The coated slide was used for multiplex antibiotic susceptibility testing of bacterial pathogens, using an algorithm-based identification of the point defining lag to exponential phase transition. It successfully determined the pathogens susceptibility profile in 3-5 hours. Finally, in Paper V, a method to retrieve bacteria colonies with a desired phenotype from the wells for downstream genetic analysis was developed. In summary, the presented work has furthered the development of miniaturised high-throughput tools for various cell heterogeneity assays.

  • 1209.
    Weibull, Emilie
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Andersson Svahn, Helene
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Ohashi, Toshiro
    A microfluidic device towards shear stress analysis on clonal expanded endothelial cells2013Manuskript (preprint) (Annet vitenskapelig)
  • 1210.
    Weibull, Emilie
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Antipas, Haris
    Richter-Dahlfors, Agneta
    Andersson Svahn, Helene
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Dynamic optical screening of single-bacterium and retrieval of the subsequent liquid colony for genetic analysis2014Manuskript (preprint) (Annet vitenskapelig)
  • 1211.
    Weibull, Emilie
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Antypas, Haris
    Kjäll, Peter
    Brauner, Annelie
    Andersson Svahn, Helene
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Richter-Dahlfors, Agneta
    Bacterial nano-scale cultures for rapid multiplexed antibiotic susceptibility testing2013Manuskript (preprint) (Annet vitenskapelig)
  • 1212.
    Weibull, Emilie
    et al.
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Matsui, S.
    Sakai, M.
    Andersson Svahn, Helene
    KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
    Ohashi, T.
    Microfluidic device for generating a stepwise concentration gradient on a microwell slide for cell analysis2013Inngår i: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 7, nr 6, s. 064115-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Understanding biomolecular gradients and their role in biological processes is essential for fully comprehending the underlying mechanisms of cells in living tissue. Conventional in vitro gradient-generating methods are unpredictable and difficult to characterize, owing to temporal and spatial fluctuations. The field of microfluidics enables complex user-defined gradients to be generated based on a detailed understanding of fluidic behavior at the μm-scale. By using microfluidic gradients created by flow, it is possible to develop rapid and dynamic stepwise concentration gradients. However, cells exposed to stepwise gradients can be perturbed by signals from neighboring cells exposed to another concentration. Hence, there is a need for a device that generates a stepwise gradient at discrete and isolated locations. Here, we present a microfluidic device for generating a stepwise concentration gradient, which utilizes a microwell slide’s pre-defined compartmentalized structure to physically separate different reagent concentrations. The gradient was generated due to flow resistance in the microchannel configuration of the device, which was designed using hydraulic analogy and theoretically verified by computational fluidic dynamics simulations. The device had two reagent channels and two dilutant channels, leading to eight chambers, each containing 4 microwells. A dose-dependency assay was performed using bovine aortic endothelial cells treated with saponin. High reproducibility between experiments was confirmed by evaluating the number of living cells in a live-dead assay. Our device generates a fully mixed fluid profile using a simple microchannel configuration and could be used in various gradient studies, e.g., screening for cytostatics or antibiotics.

  • 1213.
    Weinz, Fanny
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Behandling av cisplatinresistent lungcancer: Induktionsstudie av Gb3-uttryck hos lungcancerceller2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1214.
    Wennerholm, Julia
    Örebro universitet, Institutionen för hälsovetenskaper.
    Utvärdering av IRIS iQ200 för screening av urinprover innan odling2017Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1215. Westerberg, Ebba
    STANDARDIZATION OF PATHOGEN REDUCED POOLED SERUM FOR PRODUCTION OF EYE DROPS FOR PATIENTS WITH GRAFT-VERSUS-HOST-DISEASE2017Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
    Abstract [en]

    A large proportion of the patients who have gone through a stem cell transplantation is affected by a condition called Graft-Versus-Host-Disease, a disease which means that the transplanted immune system attacks the patient’s own cells. To treat the ocular symptoms that may occur with Graft-Versus-Host-Disease, eyedrops has been produced from allogenic serum. The serum contains a lot of the components that patients with Graft-Versus-Host-Disease are missing, such as vitamins, growth factors and fibronectin. The purpose of the study is to standardize and evaluate a method for manufacturing serum eye drops made from pooled pathogen reduced serum. The serum that has been used for the eye drops comes from 12 donors and the serum has later been pooled. The pooled serum has been pathogen reduced and transferred to eye drop containers. Tests have been done to make sure that no bacterial contamination has occurred, to measure the concentration of growth factors and also for quality controls. The results indicated no sign of bacterial contamination but enough high levels of growth factors and a good quality control with no sign of unwanted blood cells. The results therefore determined that the manufacturing of serum eye drops is successful and that they after further studies should be given to patients to mitigate the ocular symptoms of Graft-Versus-Host-Disease.

  • 1216.
    Westin, Jerker
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biomedicinsk informatik och teknik.
    Decision Support for Treatment of Patients with Advanced Parkinson’s Disease2010Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The overall aim of this thesis was to develop, deploy and evaluate new IT-based methods for supporting treatment and assessment of treatment of advanced Parkinson’s disease. In this condition a number of different motor and non-motor symptoms occur in episodes of varying frequency, duration and severity. In order to determine outcome of treatment changes, repeated assessments are necessary. Hospitalization for observation is expensive and may not be representative for the situation at home. Paper home diaries have questionable reliability and storage and retrieval of results are problematic. Approaches for monitoring using wearable sensors are unable to address important non-motor symptoms.

    A test battery system consisting of both self-assessments of symptoms and motor function tests was constructed for a touch screen mobile phone. Tests are performed on several occasions per day during test periods of one week. Data is transmitted over the mobile net to a central server where summaries in different symptom dimensions and an overall test score per patient and test period are calculated. There is a web application that graphically presents the results to treating clinical staff. As part of this work, a novel method for assessment of spiral drawing impairment useful during event-driven sampling was developed. To date, the system has been used by over 100 patients in 10 clinics in Sweden and Italy. Evidence is growing that the test battery is useful, reliable and valid for assessment of symptoms during advanced Parkinson’s disease.

    Infusion of a levodopa/carbidopa gel into the small intestine has been shown to reduce variation in plasma drug levels and improve clinical response in this patient category. A pharmacokinetic-pharmacodynamic model of this intestinal gel infusion was constructed. Possibly this model can assist the process of individualization of dosage for this treatment through in numero simulations. Results from an exploratory data analysis indicate that severity measures during oral levodopa treatment may be factors to consider when deciding candidates for infusion treatment.

  • 1217.
    Westin, Jerker
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biomedicinsk informatik och teknik.
    Dougherty, Mark
    Dalarna University.
    Nyholm, Dag
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Neurologi.
    Groth, Torgny
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biomedicinsk informatik och teknik.
    A home environment test battery for status assessment in patients with advanced Parkinson's disease2010Inngår i: Computer Methods and Programs in Biomedicine, ISSN 0169-2607, E-ISSN 1872-7565, Vol. 98, nr 1, s. 27-35Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A test battery for assessing patient state in advanced Parkinson's disease, consisting of self-assessments and motor tests, was constructed and implemented on a hand computer with touch screen in a telemedicine setting. The aim of this work was to construct an assessment device, applicable during motor fluctuations in the patient's home environment. Selection of self-assessment questions was based on questions from an e-diary, previously used in a clinical trial. Both un-cued and cued tapping tests and spiral drawing tests were designed for capturing upper limb stiffnes, slowness and involuntary movements. The patient interface gave an audible signal at scheduled response times and was locked otherwise. Data messages in an XML-format were sent from the hand unit to a central server for storage, processing and presentation. In tapping tests, speed and accuracy were calculated and in spiral tests, standard deviation of frequency filtered radial drawing velocity was calculated. An overall test score, combining repeated assessments of the different test items during a test period, was defined based on principal component analysis and linear regression. An evaluation with two pilot patients before and after receiving new types of treatments was performed. Compliance and usability was assessed in a clinical trial (65 patients with advanced Parkinson's disease) and correlations between different test items and internal consistency were investigated. The test battery could detect treatment effect in the two pilot patients, both in self-assessments, tapping tests' results and spiral scores. It had good patient compliance and acceptable usability according to nine nurses. Correlation analysis showed that tapping results provided different information as compared to diary responses. Internal consistency of the test battery was good and learning effects in the tapping tests were small.

  • 1218.
    Westin, Jerker
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biomedicinsk informatik och teknik.
    Ghiamati, Samira
    Dalarna University.
    Memedi, Mevludin
    Dalarna University.
    Nyholm, Dag
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Neurologi.
    Johansson, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Neurologi.
    Dougherty, Mark
    Dalarna University.
    Groth, Torgny
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biomedicinsk informatik och teknik.
    A new computer method for assessing drawing impairment in Parkinson's disease2010Inngår i: Journal of Neuroscience Methods, ISSN 0165-0270, E-ISSN 1872-678X, Vol. 190, nr 1, s. 143-148Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A test battery, consisting of self-assessments and motor tests (tapping and spiral drawing tasks) was used on 9482 test occasions by 62 patients with advanced Parkinson's disease (PD) in a telemedicine setting. On each test occasion, three Archimedes spirals were traced. A new computer method, using wavelet transforms and principal component analysis processed the spiral drawings to generate a spiral score. In a web interface, two PD specialists rated drawing impairment in spiral drawings from three random test occasions per patient, using a modification of the Bain & Findley 10-category scale. A standardised manual rating was defined as the mean of the two raters' assessments. Bland-Altman analysis was used to evaluate agreement between the spiral score and the standardised manual rating. Another selection of spiral drawings was used to estimate the Spearman rank correlations between the raters (r=0.87), and between the mean rating and the spiral score (r=0.89). The 95% confidence interval for the method's prediction errors was +/-1.5 scale units, which was similar to the differences between the human raters. In conclusion, the method could assess PD-related drawing impairments well comparable to trained raters.

  • 1219.
    Westin, Jerker
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Biomedicinsk informatik och teknik.
    Schiavella, Mauro
    Memedi, Mevludin
    Nyholm, Dag
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Neurologi.
    Dougherty, Mark
    Antonini, Angelo
    Validation of a home environment test battery for supporting assessments in advanced Parkinson’s disease2012Inngår i: Neurological Sciences, ISSN 1590-1874, E-ISSN 1590-3478, Vol. 33, nr 4, s. 831-838Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Test sequences in a test battery for Parkinson's disease patients, consisting of self-assessments and motor tests, were carried out repeatedly in a telemedicine setting, during week-long test periods and results were summarized in an 'overall score'. 35 patients in stable and fluctuating conditions (15 age- and gender-matched pairs) used the test battery for 1 week, and were then assessed with UPDRS and PDQ-39. This procedure was repeated 1 week later, without treatment changes. Reliability was assessed by intraclass correlation coefficients and Cronbach's alpha. Convergent validity was assessed by Spearman rank correlations and known-groups' validity, by the Mann-Whitney test. According to anonymous usability questionnaires, the patients could easily complete the tasks. Median compliance (93%) and test-retest reliability (0.88) were good. The correlations between overall score and total UPDRS (-0.64) and PDQ-39 (-0.72) were adequate. Median overall score was 18% better in the stable compared to the fluctuating group (p = 0.0014).

  • 1220.
    Wiberg, Lovisa
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Diet och nutritionsstatus påverkan på fettinlagring i njure och hjärta hos möss2018Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1221.
    Wide, Leif
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper.
    Eriksson, Karin
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper.
    Molecular size and charge as dimensions to identify and characterize circulating glycoforms of human FSH, LH and TSH2017Inngår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 122, nr 4, s. 217-223Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: FSH, LH, and TSH are glycoprotein hormones secreted from the pituitary as fully and low-asparagine-glycosylated hormones. These glycoforms of the hormones exist as a large number of isoforms varying in their glycan contents of terminal anionic monosaccharides (AMS), i.e. sialic acid (SA) and sulfonated N-acetylgalactosamine (SU). Due to the immense heterogeneity and the low concentrations in serum it has been a challenge to develop reliable analytical methods to measure and characterize the circulating glycoforms of these hormones. Methods: The hormones were separated with respect to AMS content per molecule by calibrated 0.1% agarose suspension electrophoreses. Glycoforms in separated fractions were then analyzed with respect to size by 180 calibrated Sephadex G-100 gel filtrations. The hormones were measured with timeresolved sandwich fluoroimmunoassays. All separations and assays were performed in veronal buffer at pH 8.7. Sera and fractions were also analyzed after removal of terminal SA. Results: In addition to the fully glycosylated FSH, LH, and TSH, also tri-glycosylated FSH and di-glycosylated LH and TSH forms could be identified in serum samples. The low-and fully glycosylated hormones differed both with respect to size and to median number of AMS per molecule. Algorithms, based on the distributions by electrophoreses, were developed for each hormone to estimate percent low-glycosylated forms in serum. The median numbers of SA and SU per glycoform molecule were estimated using results obtained after desialylation. Conclusion: The methods can be used for identification and characterization of glycoforms of circulating FSH, LH, and TSH in physiological and clinical studies.

  • 1222.
    Widjestam, Johan
    Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
    Kombinationsbehandling med Ceplene/IL-2 av AML-patienter i första kompletta remission2012Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1223.
    Wiklund, Johan
    et al.
    Linköpings universitet, Institutionen för systemteknik, Datorseende. Linköpings universitet, Tekniska högskolan.
    Nicolas, Vincent
    Université catholique de Louvain, Communications and Remote Sensing Lab., Place du Levant, 2, B-1348 Louvain-La-Neuve, Belgium.
    Alface, Patrice R.
    Université catholique de Louvain, Communications and Remote Sensing Lab., Place du Levant, 2, B-1348 Louvain-La-Neuve, Belgium.
    Andersson, Mats
    Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Knutsson, Hans
    Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    T-flash: Tensor Visualization in Medical Studio2009Inngår i: Tensors in Image Processing and Computer Vision, Springer London, 2009, s. 455-466Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Tensor valued data are frequently used in medical imaging. For a 3-dimensional second order tensor such data imply at least six degrees of freedom for each voxel. The operators ability to perceive this information is of outmost importance and in many cases a limiting factor for the interpretation of the data. In this paper we propose a decomposition of such tensor fields using the Tflash tensor glyphs that intuitively conveys important tensor features to a human observer. A matlab implementation for visualization of single tensors are described in detail and a VTK/ITK implementation for visualization of tensor fields have been developed as a Medical Studio component.

  • 1224.
    Wiklund, Magdalena
    Linköpings universitet, Institutionen för medicin och hälsa.
    Respons på oveckade proteiner: kan plasmid pEGFP-XBPdeldDBD-STOP-tagRFPt användas för detektion av celler vars endoplasmatiska retikulum är stressat?2016Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Bakgrund: Endoplasmatiskt retikulum upprätthåller proteinhomeostasen genom syntes och degradering av proteiner. Tre signalvägar reglerar processen via ”unfolded protein response” på olika sätt. Inositol-requiring enzyme 1 alpha är en signalväg aktiverad av en störd proteinhomeostas. Stressen uppstår om cellen utsätts för kemiska substanser t.ex. i samband med djurförsök vid framtagning av läkemedel. Den aktiverade signalvägens respons leder bl.a. till splicing av X-box-binding Protein 1 mRNA.

    Syfte: Projektets syfte är att minska behovet av djurförsök genom att möjliggöra ökad implementering av 3R-principen. Frågeställningen är om den fluorescerande plasmiden pEGFP-XBP1∆dDBD-STOP-tagRFPt som binder till X-box binding protein 1 mRNA kan användas för detektion av celler vars endoplasmatiska retikulum är stressat.

    Metod: Plasmid pEGFP-XBP1∆dDBD-STOP-tagRFPt renades ur DH5-Alpha Escherichia coli med JETstar 2.0 Plasmid Purification Midiprep Kit. Humana epiteliala njurceller 293 transfekterades med plasmiden varpå de doserades med tunicamycin. Kontrollen utgjordes av dimetylsulfoxid. Mikroskopering skedde i fluorescensmikroskopet Zeiss Axiovert 200. Mjukvaran AxioVision Ver. 4.8.1.0. Carl Zeiss Imaging Solutions framställde cellerna på dataskärm.

    Resultat: Hypotestestets signifians bestämdes till p < 0,05. Resultatet antyder att antalet ER- stressade celler ökar med ökande inkubationstid (p = 0,001 – 0,966). En mindre antydan finns också mot en ökande koncentration av tunicamycin (p = 0,096 – 0,690).

    Slutsats: En rimlig slutsats kan inte dras då antalet utförda analyser är för få. 

  • 1225. Wiklund, Urban
    et al.
    Karlsson, Marcus
    Berglin, Lena
    Högskolan i Borås, Institutionen Textilhögskolan.
    Eriksson, Siw
    Högskolan i Borås, Institutionen Textilhögskolan.
    Guo, Li
    Högskolan i Borås, Institutionen Textilhögskolan.
    Bäcklund, Tomas
    Sandsjö, Leif
    Högskolan i Borås, Institutionen Ingenjörshögskolan.
    Rytm och formanalys av EKG från textila sensorer2010Konferansepaper (Annet vitenskapelig)
  • 1226. Wiklund, Urban
    et al.
    Karlsson, Marcus
    Östlund, Nils
    Berglin, Lena
    Högskolan i Borås, Institutionen Textilhögskolan.
    Lindecrantz, Kaj
    Högskolan i Borås, Institutionen Ingenjörshögskolan.
    Karlsson, Stefan
    Sandsjö, Leif
    Högskolan i Borås, Institutionen Ingenjörshögskolan.
    Adaptive spatio-temporal filtering of disturbed ECGs: a multi-channel approach to heartbeat detection in smart clothing2007Inngår i: Medical and Biological Engineering and Computing, ISSN 0140-0118, E-ISSN 1741-0444, Vol. 45, nr 6Artikkel i tidsskrift (Fagfellevurdert)
  • 1227.
    Wikström, Daniel
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Utvärdering av Tage Justesens metod för identifiering av anaeroba bakterier2011Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1228.
    Wilenius, Kristin
    Örebro universitet, Institutionen för hälsovetenskap och medicin.
    Retrospective evaluation of the diagnostic accuracy of FluoroType® MTB assay used for detection of Mycobacterium tuberculosis in clinical samples in Greece2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1229.
    Williamson, Neil
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Phylogenetic Analysis of Rift Valley Fever using RT-PCR2015Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1230.
    Wirlander, Boris
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Makrofag migration inhibitorisk faktor (MIF) uttrycks i kolorektal cancervävnad2015Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1231.
    Wirén, Sara
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Urologi och andrologi.
    Häggström, Christel
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Urologi och andrologi.
    Ulmer, Hanno
    Manjer, Jonas
    Bjørge, Tone
    Nagel, Gabriele
    Johansen, Dorthe
    Hallmans, Göran
    Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin, Näringsforskning. Umeå universitet, Medicinska fakulteten, Enheten för biobanksforskning.
    Engeland, Anders
    Concin, Hans
    Jonsson, Håkan
    Umeå universitet, Medicinska fakulteten, Institutionen för strålningsvetenskaper, Onkologi.
    Selmer, Randi
    Tretli, Steinar
    Stocks, Tanja
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Urologi och andrologi.
    Stattin, Pär
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Urologi och andrologi.
    Pooled cohort study on height and risk of cancer and cancer death2014Inngår i: Cancer Causes and Control, ISSN 0957-5243, E-ISSN 1573-7225, Vol. 25, nr 2, s. 151-159Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    PURPOSE: To assess the association between height and risk of cancer and cancer death.

    METHODS: The metabolic syndrome and cancer project is a prospective pooled cohort study of 585,928 participants from seven cohorts in Austria, Norway, and Sweden. Hazard ratios (HRs) and 95 % confidence intervals (CIs) for cancer incidence and death were estimated in height categories and per 5-cm increment for each cancer site using Cox proportional hazards model.

    RESULTS: During a mean follow-up of 12.7 years (SD = 7.2), 38,862 participants were diagnosed with cancer and 13,547 participants died of cancer. Increased height (per 5-cm increment) was associated with an increased overall cancer risk in women, HR 1.07 (95 % CI 1.06-1.09), and in men, HR 1.04 (95 % CI 1.03-1.06). The highest HR was seen for malignant melanoma in women, HR 1.17 (95 % CI 1.11-1.24), and in men HR 1.12 (95 % CI 1.08-1.19). Height was also associated with increased risk of cancer death in women, HR 1.03 (95 % CI 1.01-1.16), and in men, HR 1.03 (95 % CI 1.01-1.05). The highest HR was observed for breast cancer death in postmenopausal women (>60 years), HR 1.10 (95 % CI 1.00-1.21), and death from renal cell carcinoma in men, HR 1.18 (95 % CI 1.07-1.30). All these associations were independent of body mass index.

    CONCLUSION: Height was associated with risk of cancer and cancer death indicating that factors related to height such as hormonal and genetic factors stimulate both cancer development and progression.

  • 1232.
    Woldu Haddish, Eden
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Autophagy-related protein expression in atrophic and hypertrophic denervated skeletal muscles2013Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1233.
    Wollert, Kai C.
    et al.
    Hannover Med Sch, Dept Cardiol & Angiol, Div Mol & Translat Cardiol, Hannover, Germany..
    Kempf, Tibor
    Hannover Med Sch, Dept Cardiol & Angiol, Div Mol & Translat Cardiol, Hannover, Germany..
    Wallentin, Lars
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Kardiologi. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Uppsala kliniska forskningscentrum (UCR).
    Growth Differentiation Factor 15 as a Biomarker in Cardiovascular Disease2017Inngår i: Clinical Chemistry, ISSN 0009-9147, E-ISSN 1530-8561, Vol. 63, nr 1, s. 140-151Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    BACKGROUND: Growth differentiation factor 15 (GDF-15) is expressed and secreted in response to inflammation, oxidative stress, hypoxia, telomere erosion, and oncogene activation. Cardiovascular (CV) disease is a major. driver of GDF-15 production. GDF-15 has favorable preanalytic characteristics and can be measured in serum and plasma by immunoassay. CONTENT: In community-dwelling individuals higher concentrations of GDF-15 are associated with increased risks of developing CV disease, chronic kidney disease, and cancer, independent of traditional CV risk factors, renal function, and other biomarkers (C-reactive protein, B-type natriuretic peptide, cardiac troponin). Low concentrations of GDF-15 are closely associated with longevity. GDF-15 is as an independent marker of all-cause mortality and CV events in patients with coronary artery disease, and may help select patients with non-ST-elevation acute coronary syndrome for early revascularization and more intensive medical therapies. GDF-15 is, independently associated with mortality and nonfatal events in atrial fibrillation and heart failure (HF) with preserved or reduced ejection fraction. GDF-15 reflects chronic disease burden and acute perturbations in HF and responds to improvements in hemodynamic status. GDF-15 is independently associated with major bleeding in patients receiving antithrombotic therapies and has been included in a new bleeding risk score, which may become useful for decision support. SUMMARY: GDF-15 captures distinct aspects of CV disease development, progression, and prognosis, which are not represented by clinical risk predictors and other biomarkers. The usefulness of GDF-15 to guide management decisions and discover new treatment targets should be further explored.

  • 1234.
    Wrangsjö, Andreas
    et al.
    Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Knutsson, Hans
    Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Histogram Filters for Noise Reduction2003Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    A class of filters based on histograms are presented. The signal probability density function is estimated and filtering is performed in the pdf domain. Such filters can be designed to preserve signal features such as sharp edges while suppressing stochastic variations. One particular histogram filter scheme is evaluated and compared to a median filter and a normal gaussian blurring filter.

  • 1235. Wulff, John
    et al.
    Lönn, Urban
    Keck, Karl Yngve
    Wranne, Bengt
    Linköpings universitet, Institutionen för medicin och vård, Klinisk fysiologi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Hjärt- och Medicincentrum, Fysiologiska kliniken US.
    Ask, Per
    Linköpings universitet, Institutionen för medicinsk teknik, Fysiologisk mätteknik. Linköpings universitet, Tekniska högskolan.
    Peterzén, Bengt
    Casimir-Ahn, Henrik
    Flow characteristics of the Hemopump: an experimental in vitro study.1997Inngår i: Annals of Thoracic Surgery, ISSN 0003-4975, E-ISSN 1552-6259, Vol. 63, nr 1, s. 162-166Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    BACKGROUND: The Hemopump (DLP/Medtronic) has been in clinical use for about 7 years. There is still no adequate way of determining actual output from the three available pump systems in the clinical situation. If the pump is completely stopped during weaning from the device, there is a possibility of back-leakage through the pump, endangering the patient from regurgitation into the left ventricle. It can also make it more difficult to judge the recovery of heart function because of a volume load of the left ventricle. The aim of this study was to evaluate in a standardized, experimental in vitro model the output from three different-sized Hemopump catheters at various pressure levels and to quantify the back-flow through the pumps.

    METHODS: The Hemopump models were tested in an in vitro study regarding total outflow at various speeds at three pressure levels. The back-flow through the pumps was also measured with the pumps at a complete stop.

    RESULTS: The outflow from the Hemopumps ranged from 0.4 to 4.5 L/min, depending on which pump and speed were used. Variations in total output, depending on speed and various pressure settings, could be up to 0.4 L/min. Back-flow through the pump into the left ventricle may be as great as 1.6 L/min.

    CONCLUSIONS: The flow outputs from the different Hemopump models were reproducible over time and were closely related to the resistance of the model. The Hemopump, if not running, can induce substantial regurgitation through the pump into the left ventricle.

  • 1236.
    Wårdell, Karin
    et al.
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska högskolan.
    Blomstedt, P.
    Department of Neurosurgery, University Hospital, Umeå, Sweden.
    Richter, Johan
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik.
    Antonsson, Johan
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik.
    Eriksson, Ola
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik.
    Zsigmond, Peter
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Neurokirurgi. Östergötlands Läns Landsting, Rekonstruktionscentrum, Neurokirurgiska kliniken US.
    Bergenheim, A.T.
    Department of Neurosurgery, University Hospital, Umeå, Sweden.
    Hariz, M.I.
    Department of Neurosurgery, University Hospital, Umeå, Sweden, Institute of Neurology, University College London, London, United Kingdom.
    Intracerebral microvascular measurements during deep brain stimulation implantation using laser doppler perfusion monitoring2007Inngår i: Stereotactic and Functional Neurosurgery, ISSN 1011-6125, E-ISSN 1423-0372, Vol. 85, nr 6, s. 279-286Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of the study was to investigate if laser Doppler perfusion monitoring (LDPM) can be used in order to differentiate between gray and white matter and to what extent microvascular perfusion can be recorded in the deep brain structures during stereotactic neurosurgery. An optical probe constructed to fit in the Leksell® Stereotactic System was used for measurements along the trajectory and in the targets (globus pallidus internus, subthalamic nucleus, zona incerta, thalamus) during the implantation of deep brain stimulation leads (n = 22). The total backscattered light intensity (TLI) reflecting the grayness of the tissue, and the microvascular perfusion were captured at 128 sites. Heartbeat-synchronized pulsations were found at all perfusion recordings. In 6 sites the perfusion was more than 6 times higher than the closest neighbor indicating a possible small vessel structure. TLI was significantly higher (p < 0.005) and the perfusion significantly lower (p < 0.005) in positions identified as white matter in the respective MRI batch. The measurements imply that LDPM has the potential to be used as an intracerebral guidance tool.

  • 1237.
    Wårdell, Karin
    et al.
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV.
    Hemm-Ode, Simone
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska fakulteten. University of Applied Sciences and Arts Northwestern Switzerland, Muttenz , Switzerland.
    Rejmstad, Peter
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska fakulteten.
    Zsigmond, Peter
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för neuro- och inflammationsvetenskap. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Sinnescentrum, Neurokirurgiska kliniken US.
    High-Resolution Laser Doppler Measurements of Microcirculation in the Deep Brain Structures: A Method for Potential Vessel Tracking.2016Inngår i: Stereotactic and Functional Neurosurgery, ISSN 1011-6125, E-ISSN 1423-0372, Vol. 94, nr 1, s. 1-9Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    BACKGROUND: Laser Doppler flowmetry (LDF) can be used to measure cerebral microcirculation in relation to stereotactic deep brain stimulation (DBS) implantations.

    OBJECTIVE: To investigate the microcirculation and total light intensity (TLI) corresponding to tissue grayness in DBS target regions with high-resolution LDF recordings, and to define a resolution which enables detection of small vessels.

    METHODS: Stereotactic LDF measurements were made prior to DBS implantation with 0.5-mm steps in the vicinity to 4 deep brain targets (STN, GPi, Vim, Zi) along 20 trajectories. The Mann-Whitney U test was used to compare the microcirculation and TLI between targets, and the measurement resolution (0.5 vs. 1 mm). The numbers of high blood flow spots along the trajectories were calculated.

    RESULTS: There was a significant difference (p < 0.05) in microcirculation between the targets. High blood flow spots were present at 15 out of 510 positions, 7 along Vim and GPi trajectories, respectively. There was no statistical difference between resolutions even though both local blood flow and TLI peaks could appear at 0.5-mm steps.

    CONCLUSIONS: LDF can be used for online tracking of critical regions presenting blood flow and TLI peaks, possibly relating to vessel structures and thin laminas along stereotactic trajectories.

  • 1238.
    Xie, Li
    et al.
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Elektroniksystem. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    Yang, Geng
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Elektroniksystem. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    Mantysalo, Matti
    TUT.
    Jonsson, Fredrik
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Elektroniksystem. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    Zheng, Li-Rong
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Elektroniksystem. KTH, Skolan för informations- och kommunikationsteknik (ICT), Centra, VinnExcellence Center for Intelligence in Paper and Packaging, iPACK.
    A system-on-chip and paper-based inkjet printed electrodes for a hybrid wearable bio-sensing system2012Inngår i: Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE, IEEE , 2012, s. 5026-5029Konferansepaper (Fagfellevurdert)
    Abstract [en]

    This paper presents a hybrid wearable bio-sensing system, which combines traditional small-area low-power and high-performance System-on-Chip (SoC), flexible paper substrate and cost-effective Printed Electronics. Differential bio-signals are measured, digitized, stored and transmitted by the SoC. The total area of the chip is 1.5 × 3.0 mm2. This enables the miniaturization of the wearable system. The electrodes and interconnects are inkjet printed on paper substrate and the performance is verified in in-vivo tests. The quality of electrocardiogram signal sensed by printed electrodes is comparable with commercial electrodes, with noise level slightly increased. The paper-based inkjet printed system is flexible, light and thin, which makes the final system comfortable for end-users. The hybrid bio-sensing system offers a potential solution to the next generation wearable healthcare technology.

  • 1239. Yaka, Cane
    Studies of axonal regeneration on a grid pattern of extracellular matrix proteins2014Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1240.
    Yang, Xianyan
    et al.
    Zhejiang Univ, Zhejiang Calif Int Nanosyst Inst, Bionanomat & Regenerat Med Res Div, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China..
    Xu, Sanzhong
    Zhejiang Univ, Affiliated Hosp 1, Dept Orthopaed Surg, Sch Med, Hangzhou 310003, Zhejiang, Peoples R China..
    Chen, Xiaoyi
    Zhejiang Univ, Zhejiang Calif Int Nanosyst Inst, Bionanomat & Regenerat Med Res Div, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China.;Zhejiang Prov Peoples Hosp, Clin Res Inst, Hangzhou 310014, Zhejiang, Peoples R China..
    He, Dongshuang
    Zhejiang Univ, Zhejiang Calif Int Nanosyst Inst, Bionanomat & Regenerat Med Res Div, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China..
    Ke, Xiurong
    Wenzhou Med Univ, Ruian Peoples Hosp, Ruian 325200, Peoples R China.;Wenzhou Med Univ, Hosp Affiliated 3, Ruian 325200, Peoples R China..
    Zhang, Lei
    Wenzhou Med Univ, Ruian Peoples Hosp, Ruian 325200, Peoples R China.;Wenzhou Med Univ, Hosp Affiliated 3, Ruian 325200, Peoples R China..
    Yang, Guojing
    Wenzhou Med Univ, Ruian Peoples Hosp, Ruian 325200, Peoples R China.;Wenzhou Med Univ, Hosp Affiliated 3, Ruian 325200, Peoples R China..
    Liu, An
    Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Orthopaed Surg, Hangzhou 310009, Zhejiang, Peoples R China..
    Mou, Xiaozhou
    Zhejiang Prov Peoples Hosp, Clin Res Inst, Hangzhou 310014, Zhejiang, Peoples R China..
    Xia, Wei
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Tillämpad materialvetenskap.
    Gou, Zhongru
    Zhejiang Univ, Zhejiang Calif Int Nanosyst Inst, Bionanomat & Regenerat Med Res Div, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China..
    Intra-bone marrow injection of trace elements co-doped calcium phosphate microparticles for the treatment of osteoporotic rat2017Inngår i: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965, Vol. 105, nr 5, s. 1422-1432Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Osteoporotic femur fractures are the most common fragility fracture and account for approximately one million injuries per year. Local intervention by intra-marrow injection is potentially a good choice for preventing osteoporotic bone loss when the osteoporotic femoral fracture was treated. Previously, it was shown that trace element co-doped calcium phosphate (teCaP) implants could stimulate osteoporotic bone marrow mesenchymal stem cell activity in vitro and bone regeneration in femoral bone defects in osteoporotic animal models. They hypothesized that local intra-marrow injection of teCaP particles could improve bone function because the teCaP can sustain release of biologically essential inorganic minerals and improve bone remodeling in osteoporosis. The teCaP and CaP particles were synthesized in simulated body fluid with and without adding silicon, zinc and strontium ions. Female rats (8 months) were ovariectomized (OVX) or sham-operated, and then intervened in the femoral marrow space at 12 months old. Groups include: (1) saline water; (2) CaP particles; and (3) teCaP particles. After 2-3 months of intervention, the sham groups showed higher bone mineral density (MBD) in the femur, and teCaP group increased the BMD in the OVX groups. The compressive strength of the OVX-teCaP group was significantly higher than that in the OVX-CaP group. Significant differences between OVX-teCaP and OVX-CaP groups were found for bone mineral microarchitecture, bone mineral density, and trace mineral content, but not for feces composition. These results confirm the teCaP particles could suppress osteoporotic bone loss by local intramarrow injection. Therefore, this biomaterial could be used as a next-generation combination treatment for osteoporotic trauma and osteoporosis itself.

  • 1241. Yassin, M. A.
    et al.
    Leknes, K. N.
    Sun, Yang
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknologi.
    Lie, S. A.
    Finne-Wistrand, Anna
    KTH, Skolan för kemivetenskap (CHE), Fiber- och polymerteknologi.
    Mustafa, K.
    Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation2016Inngår i: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Poly(l-lactide-co-e(open)-caprolactone) (poly(LLA-co-CL)) has been blended with Tween 80 to tune the material properties and optimize cell-material interactions. Accordingly, the aims of this study were fourfold: to evaluate the effect of low concentrations of Tween 80 on the surface microstructure of 3D poly(LLA-co-CL) porous scaffolds: to determine the effect of different concentrations of Tween 80 on proliferation of bone marrow stromal cells (BMSCs) in vitro under dynamic cell culture at 7 and 21 days; to assess the influence of Tween 80 on the degradation rate of poly(LLA-co-CL) at 7 and 21 days; and in a subcutaneous rat model, to evaluate the effect on bone formation of porous scaffolds modified with 3% Tween 80 at 2 and 8 weeks. Blending 3% (w/w) Tween 80 with poly(LLA-co-CL) improves the surface wettability (p<0.001). Poly(LLA-co-CL)/3% Tween 80 shows significantly increased cellular proliferation at days 7 and 21 (p<0.001). Moreover, the presence of Tween 80 facilitates the degradation of poly(LLA-co-CL). Two weeks post-implantation, the poly(LLA-co-CL)/3% Tween 80 scaffolds exhibit significant mRNA expression of Runx2 (p=0.004). After 8 weeks, poly(LLA-co-CL)/3% Tween 80 scaffolds show significantly increased de novo bone formation, demonstrated by μ-CT (p=0.0133) and confirmed histologically. It can be concluded that blending 3% (w/w) Tween 80 with poly (LLA-co-CL) improves the hydrophilicity and osteogenic potential of the scaffolds.

  • 1242.
    Ylinenjärvi, Cecilia
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Galleria mellonella som modellsystem för anrikning och identifiering av Francisella i miljöprover2017Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1243.
    Yuan, Tian
    Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för hälsovetenskaper.
    Effects  of  Ellagic  Acid  in  Human Bronchial Epithelial Cells: Effects on Tumor Necrosis Factor-α  Induced Inflammatory Mediators2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1244.
    Zajac, Jakub
    Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för kardiovaskulär medicin. Linköpings universitet, Medicinska fakulteten.
    Assessment of Ventricular Function in Normal and Failing Hearts Using 4D Flow CMR2017Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Heart failure is a common disorder and a major cause of illness and death in the population, creating an enormous health-care burden. It is a complex condition, representing the end-point of many cardiovascular diseases. In general heart failure progresses slowly over time and once it is diagnosed it has a poor prognosis which is comparable with that of many types of cancer.

    The heart has an ability to adapt in response to long lasting increases in hemodynamic demand; the heart conforms its shape and size in order to maintain adequate cardiac output. This process is called remodeling and can be triggered by pathologies such as hypertension or valvular disease. When the myocardial remodeling is maintained chronically it becomes maladaptive and is associated with an increased risk of heart failure.

    In many cases, heart failure is associated with left bundle branch block (LBBB). This electrical disturbance leads to dyssynchronous left ventricular (LV) contraction and relaxation which may contribute to cardiac dysfunction and ultimately heart failure. Mechanical dyssynchrony can be treated with cardiac resynchronization therapy (CRT). However, many heart failure patients do not demonstrate clinical improvement despite CRT.

    Blood flow plays an important role in the normal development of the fetal heart. However, flow-induced forces may also induce changes in the heart cells that could lead to pathological remodeling in the adult heart. Until recently, measurement tools have been inadequate in describing the complex three-dimensional and time-varying characteristics of blood flow within the beating heart.

    4D (3D + time) flow cardiovascular magnetic resonance (CMR) enables acquisition of three-dimensional, three-directional, time-resolved velocity data from which visualization and quantification of the blood flow patterns over a complete cardiac cycle can be performed. In this thesis, novel 4D Flow CMR based methods are used to study the intraventricular blood flow in healthy subjects and heart failure patients with and without ventricular dyssynchrony in order to gain new knowledge of the ventricular function.

    Different flow components were assessed in normal heart ventricles. It was found that inflowing blood that passes directly to outflow during the same heartbeat (the Direct Flow component) was larger and possessed more kinetic energy (KE) than other flow components. Diastolic flow through the normal heart appears to create favorable conditions for effective systolic ejection. This organized blood flow pattern within the normal LV is altered in heart failure patients and is associated with decreased preservation of KE which might be unfavorable for efficient LV ejection. Inefficient flow of blood through the heart may influence diastolic wall stress, and thus contribute to pathological myocardial remodeling.

    In dyssynchronous LVs of heart failure patients with LBBB, Direct Flow showed even more reduced preservation of KE compared to similarly remodeled LVs without LBBB. Furthermore, in LBBB patients, LV filling hemodynamic forces, acting on the myocardium, were more orthogonal to the main flow direction compared to patients without LBBB. Deviation of LV flow forces and reduction of KE preservation and may reflect impairment of LV diastolic function and less efficient ensuing ejection related to dyssynchrony in these failing ventricles.

    Blood flow patterns were also studied with respect to fluctuations of the velocity of the flow (turbulent flow) in normal and failing LVs. In failing hearts, turbulent kinetic energy (TKE) was higher during diastole than in healthy subjects. TKE is a cause of energy loss and can thus be seen as a measure of flow inefficiency.

    Elucidating the transit of multidimensional blood flow through the heart chambers is fundamental in understanding the physiology of the heart and to detect abnormalities in cardiac function. The 4D Flow CMR parameters presented in this thesis can be utilized to detect altered intracardiac blood flow and may be used as markers of deteriorating cardiac function, pathological remodeling and mechanical dyssynchrony in heart failure.

  • 1245.
    Zamannoun, Sarah
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Biomedicinsk laboratorievetenskap.
    Distensibilitet i karotisartären hos patienter med nydiagnostiserad rheumatoid artrit jämfört med patienter med längre sjukdomsduration: En klinisk och metodologisk studie2016Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
  • 1246.
    Zhang, Hanqian
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi.
    Ericsson, Maja
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi.
    Virtanen, Marie
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi.
    Weström, Simone
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi.
    Wählby, Carolina
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Institutionen för informationsteknologi, Avdelningen för visuell information och interaktion. Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Matematisk-datavetenskapliga sektionen, Institutionen för informationsteknologi, Bildanalys och människa-datorinteraktion.
    Vahlquist, Anders
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi.
    Törmä, Hans
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinska vetenskaper, Dermatologi och venereologi.
    Quantitative image analysis of protein expression and colocalisation in skin sections2018Inngår i: Experimental dermatology, ISSN 0906-6705, E-ISSN 1600-0625, Vol. 27, nr 2, s. 196-199Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Immunofluorescence (IF) and in situ proximity ligation assay (isPLA) are techniques that are used for in situ protein expression and colocalisation analysis, respectively. However, an efficient quantitative method to analyse both IF and isPLA staining on skin sections is lacking. Therefore, we developed a new method for semi-automatic quantitative layer-by-layer measurement of protein expression and colocalisation in skin sections using the free open-source software CellProfiler. As a proof of principle, IF and isPLA of ichthyosis-related proteins TGm-1 and SDR9C7 were examined. The results indicate that this new method can be used for protein expression and colocalisation analysis in skin sections.

  • 1247.
    Zhou, Yiwen
    Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), Institutionen för hälsovetenskaper.
    Effect of the exposure to phthalates on phagocytotic activity in non-differentiated and differentiated THP-1 cells2013Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 1248.
    Zieba, Agata
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Molekylära verktyg.
    Application of Proximity Ligation Assay for Multidirectional Studies on Transforming Growth Factor-β Pathway2012Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    A comprehensive understanding of how the body and all its components function is essential when this knowledge is exploited for medical purposes. The achievements in biological and medical research during last decades has provided us with the complete human genome and identified signaling pathways that governs the cellular processes that facilitates the development and maintenance of higher order organisms. This has brought about the realization that diseases such as cancer is a consequence of genomic aberrations that effects these signaling pathways, endowing cancer cells with the capacity to circumvent homeostasis by acquiring features like self-sustained proliferation and insensitivity to apoptosis. The increased understanding of biology and medicine has been made possible by the development of advanced methods to carry out biological and clinical analyses. The demands of a method often differ regarding in what context it will be applied. It may be acceptable for method to be laborious and time consuming if it is used in basic research, but for medical purposes molecular methods need to be fast and straightforward to perform. Innovative technologies should preferentially address the demands of both researchers and clinicians and provide data not possible to obtain by other methods. An example of such a method is the in situ proximity ligation assay (in situ PLA). In this thesis I have used this method to determine the activity status, at the single-cell level, of the transforming growth factor-β (TGF-β) signaling pathway and activating protein-1 (AP-1) family of transcription factors.  Both of these pathways are frequently involved in cancer development and progression. In addition to this research I herein also present further modifications of in situ PLA, and analyses thereof, to increase the utility and resolution of this assay.

  • 1249.
    Ziethén, Kristina
    Högskolan Kristianstad, Sektionen för lärande och miljö.
    Validering av turbidimetrisk metod för koncentrationsbestämning av albumin i cerebrospinalvätska2018Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

     I denna studie jämfördes två olika metoder för att analysera cerbrospinalvätska (CSF); spinal-protein och spinal-albumin. Syftet med studien var att undersöka om spinal-albumin som baseras på turbidimetri skulle kunna ersätta spinal-protein som baseras på spektrofotometri. Denna används idag som rutinanalys på laboratoriet för klinisk kemi på S Södra Älvsborgs sjukhus (SÄS). 35 prover analyserades, tagna från det CSF -prover som ankom till SÄS. Varje prov kördes två gånger med respektive metod. Studien visade en god korrelation mellan metoderna, dock visade Bland-Altman diagram mindre spridning av värdena som erhölls med albumin-metoden. Resultaten för albumin jämfördes mot Sahlgrenska universitetssjukhus (SU) resultat, då albumin-metoden ingår i deras rutiner.  

    Studien visade också att de dagliga kontrollerna som kördes ej var lämpade för albumin metoden, då både innehåll och koncentration inte var anpassad till spinalprover. Kontrollerna kommer att bytas ut mot andra mer lämpade kontroller. Metoden för spinal-albumin kommer att kunna ersätta total-protein som rutinanalys av CFS.

  • 1250.
    Zulj, Jelena
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
    Verifiering av metod för analys av tumörmarkörerna CA 125, CA 15-3 och CA 19-9 på Roche Cobas e4112014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    Tumörmarkörer är substanser som frisätts i kroppsvätskor från cancerceller. Markörerna används inom sjukvården för att upptäcka och följa upp maligna sjukdomar med analyser av markörerna i serum/plasma. Tre av dessa är cancerantigenerna CA 125, CA 15-3 och CA 19-9. Syftet med studien var att verifiera analysmetoden för tumörmarkörerna CA 125, CA 15-3 och CA 19-9 på två Roche Cobas e411 instrument (instrument 1 och 2) inför införandet av dessa vid avdelningen för klinisk kemi och transfusionsmedicin, Lnadstinget Kalmar län. De av företaget rekommenderade cut-off värdena på Roche Cobas e411 (Roche Diagnostics) är för CA 125 <35 kU/l, för CA 15-3 ≤25 kU/l och för CA 19-9 <27 kU/l. Precisionen beräknades med hjälp av statistiska metoder genom analys av den mellanliggande precisionen (mätning av två kontrollnivåer under 5 dagar) och repeterbarheten (analys av två kontrollnivåer i en serie). En korrelationsstudie gjordes med patientprover som erhölls från Aleris Medilab (Abbot Architect i System). Den mellanliggande precisionenn resulterade i högre variationskoefficientvärden (CV %) för samtliga tre markörer i förhållande till ett åsatt CV från Roche Diagnostics. CV värdet skilde även mellan de två Roche Cobas e411 instrumenten. Repeterbarheten bedömdes vara acceptabel för samtliga tre markörer. Korrelationsstudien visade en skillnad i de uppmätta värdena mellan Roche Cobas e411 och Abbot Architect för samtliga tre markörer. Då Roche Cobas e411 tenderade att ge högre uppmätta värden (kU/l) (54 av 85 gånger). Samstämmigheten mellan metoderna var bra då det endast var två prover vid analys av CA 15-3 och två prover vid analys av CA 19-9 som var på skilda sidor om cut-off värdet. Sammanfattningsvis visade studien att den mellanliggande precisisonen (CV-värdet) för alla tre markörerna var högre än Roche Diagnostics angivna CV värde. Olika CV värden erhölls med Roche Cobas e411 jämfört med Abbot Architect. Olika CV värden erhölls också med instrument 1 jämfört med instrument 2. Precisionen anses vara tillräckligt god för införandet av metoderna i rutinbruk.

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