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  • 1.
    Bagge, Joakim
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Hedman, Erik
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Smedsrud, Sabina
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Svärdström, Cornelia
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Söderberg, Elisabeth
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Valdez, Fernando
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Utveckling av metodik för påvisning och typning av Listeria i livsmedelskedjan2017Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
  • 2.
    Bengtsson, Katarina
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Design of a galvanotaxic track for cells, using polymer electrodes.2011Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
    Abstract [en]

    Galvanotaxis is the movement of cells in an applied electric field. The first steps to design a chip for observations of galvanotaxic behavior of cells were done in this work. The chip is a miniaturised system of previous larger galvanotaxis systems and uses materials which are thought to be biocompatible. The system was constructed on microscope slides with a channel in PDMS with adjacent polymer electrodes. The polymer electrodes were made from poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), glycerol and Silquest A-187. The PEDOT:PSS electrodes were connected with either an evaporated metal electrode of titanium and gold or a gold net. Systems with PEDOT:PSS are neutralised when put in excessive amount of PBS (pH=7.4) for 24 hours. The final system had a channel with dimension length=14 mm, width=0.5 mm and height=0.25 mm. PEDOT:PSS worked as an electrode material and the achieved electric field through the channel was between 55 V/m and 160 V/m with an applied voltage of 1 V. The decrease of the electric field within the first hour was between 10 % and 30%.  Further development of this system could give an easy way to observe galvanotaxic behaviour of cells or an instrument that can distinguish between different cell types.

  • 3.
    Cecilia, Bill
    Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Hälsouniversitetet.
    Improving anti-drug antibody assay performance in Gyrolab for therapeutic recombinant antibody Infliximab2014Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
    Abstract [en]

    Monoclonal antibodies can be used as targeting therapies for several diseases. One major concern when using these therapies is anti-drug antibodies which may hamper the drugs efficiency. Gyrolab is an automated platform which can be used to develop bridging immunoassays where the anti-drug antibodies affinity towards the monoclonal antibody is utilized. Anti-drug antibody immunoassay development on Gyrolab is limited mainly by three factors which may inappropriately affect signal intensity levels. In this project different variants of bridging immunoassays based on drug Fab fragments have been developed for monoclonal antibody Infliximab, with the purpose to illustrate the effects of these three factors.

    Findings indicate that an assay based completely on drug Fab fragments is more sensitive compared to an assay based on intact drug since less affected by unspecific interactions between drug reagents and complex formations. Surprisingly findings also indicate that an assay based completely on drug Fab fragments is affected by human anti-hinge antibodies which decrease assay sensitivity. The most optimal assay variant is based on the combination between intact capture drug and Fab fragment as detection. This variant is insensitive to false positive reactions caused by Rheumatoid factor and human anti-hinge antibodies, less prone to form unspecific interactions between drug reagents and complex formations in the presence of anti-drug antibodies. The optimal assay variant also demonstrates best drug tolerance in combination with acid dissociation.

  • 4.
    Dev, Apurba
    et al.
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Material- och nanofysik. Uppsala University, Sweden.
    Horak, J.
    Kaiser, A.
    Yuan, X.
    Perols, A.
    Björk, P.
    Karlström, A. E.
    Kleimann, P.
    Linnros, Jan
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Material- och nanofysik, Materialfysik, MF.
    Electrokinetic effect for molecular recognition: A label-free approach for real-time biosensing2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 82, 55-63 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present a simple and inexpensive method for label-free detection of biomolecules. The method monitors the changes in streaming current in a fused silica capillary as target biomolecules bind to immobilized receptors on the inner surface of the capillary. To validate the concept, we show detection and time response of different protein-ligand and protein-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z domain-immunoglobulin G (IgG). We show that specific binding of these biomolecules can be reliably monitored using a very simple setup. Using sequential injections of various proteins at a diverse concentration range and as well as diluted human serum we further investigate the capacity of the proposed technique to perform specific target detection from a complex sample. We also investigate the time for the signal to reach equilibrium and its dependence on analyte concentration and demonstrate that the current setup can be used to detect biomolecules at a concentration as low as 100 pM without requiring any advanced device fabrication procedures. Finally, an analytical model based on diffusion theory has been presented to explain the dependence of the saturation time on the analyte concentration and capillary dimensions and how reducing length and inner diameter of the capillary is predicted to give faster detection and in practice also lower limit of detection. © 2016 Elsevier B.V.

  • 5.
    Iredahl, Fredrik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för kliniska vetenskaper. Linköpings universitet, Medicinska fakulteten.
    Assessment of microvascular and metabolic responses in the skin2016Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The general aim of this project was to develop experimental in vivo models that allow for minimally invasive investigations of responses in the skin to microvascular and metabolic provocations. The cutaneous microvasculature has emerged as a valuable model and been proposed to mirror the microcirculation in other organs. Dysfunction in the cutaneous microcirculation has thus been linked to systemic diseases such as hypertension and diabetes mellitus. Models for investigating skin responses could facilitate the understanding of pathophysiological mechanisms as well as effects of drugs.

    In the first study, three optical measurement techniques (laser Doppler flowmetry (LDF), laser speckle contrast imaging (LSCI) and tissue viability imaging (TiVi)) were compared against each other and showed differences in their ability to detect microvascular responses to provocations in the skin. TiVi was found more sensitive for measurement of noradrenaline-induced vasoconstriction, while LSCI was more sensitive for measurement of vascular occlusion. In the second study, microvascular responses in the skin to iontophoresis of vasoactive drugs were found to depend on the drug delivery protocol. Perfusion half-life was defined and used to describe the decay in the microvascular response to a drug after iontophoresis. In the third study, the role of nitric oxide (NO) was assessed during iontophoresis of insulin. The results showed a NO-dependent vasodilation in the skin by insulin. In the fourth study the vasoactive and metabolic effects of insulin were studied after both local and endogenous administration. Local delivery of insulin increased skin blood flow, paralleled by increased skin concentrations of interstitial pyruvate and lactate, although no change in glucose concentration was observed. An oral glucose load resulted in an increased insulin concentration in the skin paralleled by an increase in blood flow, as measured using the microdialysis urea clearance technique, although no changes in perfusion was measured by LSCI.

    The thesis concludes that when studying skin microvascular responses, the choice of measurement technique and the drug delivery protocol has an impact on the measurement results, and should therefore be carefully considered. The thesis also concludes that insulin has metabolic and vasodilatory effects in the skin both when administered locally and as an endogenous response to an oral glucose load. The vasodilatory effect of insulin in the skin is mediated by nitric oxide.

  • 6.
    Johansson, Johannes D.
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik.
    Fredriksson, Ingemar
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska högskolan.
    Wårdell, Karin
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska högskolan.
    Eriksson, Ola
    Linköpings universitet, Institutionen för medicinsk teknik, Biomedicinsk instrumentteknik. Linköpings universitet, Tekniska högskolan.
    Simulation of reflected light intensity changes during navigation and radio frequency lesioning in the brain2009Inngår i: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 14, nr 044040Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    An electrode with adjacent optical fibers for measurements during navigation and radio frequency lesioning in the brain was modeled for Monte Carlo simulations of light transport in brain tissue. Relative reflected light intensity at 780 nm, I780, from this electrode and probes with identical fiber configuration were simulated using the intensity from native white matter as reference. Models were made of homogeneousnative and coagulated gray, thalamus, and white matter as well as blood. Dual layermodels, including models with a layer of cerebrospinal fluid between the fibers andthe brain tissue, were also made. Simulated I780 was 0.16 for gray matter, 0.67 forcoagulate gray matter, 0.36 for thalamus, 0.39 for coagulated thalamus, unity forwhite matter, 0.70 for coagulated white matter and 0.24 for blood. Thalamic matterhas also been found to reflect more light than gray matter and less than white matterin clinical studies. In conclusion the reflected light intensity can be used todifferentiate between gray and white matter during navigation. Furthermore,coagulation of light gray tissue, such as the thalamus, might be difficult to detectusing I780, but coagulation in darker gray tissue should result in a rapid increase of I780.

  • 7.
    Nordesjö, Olle
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Pontén, Victor
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Herman, Stephanie
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Ås, Joel
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Jamal, Sabri
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Nyberg, Alona
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning.
    Ett sannolikhetsbaserat kvalitetsmått förbättrar klassificeringen av oförväntade sekvenser i in situ sekvensering2014Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
    Abstract [sv]

    In situ sekvensering är en metod som kan användas för att lokalisera differentiellt uttryck av mRNA direkt i vävnadssnitt, vilket kan ge viktiga ledtrådar om många sjukdomstillstånd. Idag förloras många av sekvenserna från in situ sekvensering på grund av det kvalitetsmått man använder för att säkerställa att sekvenser är korrekta. Det finns troligtvis möjlighet att förbättra prestandan av den nuvarande base calling-metoden eftersom att metoden är i ett tidigt utvecklingsskede. Vi har genomfört explorativ dataanalys för att undersöka förekomst av systematiska fel och korrigerat för dessa med hjälp av statistiska metoder. Vi har framförallt undersökt tre metoder för att korrigera för systematiska fel:

    I) Korrektion av överblödning som sker på grund avöverlappande emissionsspektra mellan fluorescenta prober.

    II) En sannolikhetsbaserad tolkningav intensitetsdata som resulterar i ett nytt kvalitetsmått och en alternativ klassificerare baseradpå övervakad inlärning.

    III) En utredning om förekomst av cykelberoende effekter, exempelvisofullständig dehybridisering av fluorescenta prober.

    Vi föreslår att man gör följande saker:

    • Implementerar och utvärderar det sannolikhetsbaserade kvalitetsmåttet
    • Utvecklar och implementerar den föreslagna klassificeraren
    • Genomför ytterligare experiment för att påvisa eller bestrida förekomst av ofullständigdehybridisering
  • 8.
    Valenti, Marco
    et al.
    Delft University of Technology, Netherlands.
    Kontoleta, Evgenia
    Delft University of Technology, Netherlands.
    Digdaya, Ibadillah A.
    Delft University of Technology, Netherlands.
    Jonsson, Magnus
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Tekniska fakulteten.
    Biskos, George
    Delft University of Technology, Netherlands; Cyprus Institute, Cyprus.
    Schmidt-Ott, Andreas
    Delft University of Technology, Netherlands.
    Smith, Wilson A.
    Delft University of Technology, Netherlands.
    The Role of Size and Dimerization of Decorating Plasmonic Silver Nanoparticles on the Photoelectrochemical Solar Water Splitting Performance of BiVO4 Photoanodes2016Inngår i: CHEMNANOMAT, ISSN 2199-692X, Vol. 2, nr 7, 739-747 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Ag nanoparticles (NPs) are deposited on BiVO4 photoanodes to study their effect on the photoelectrochemical (PEC) water splitting performance of the semiconductor. 15 nm light-absorbing NPs and 65 nm light scattering NPs were studied separately to compare their light trapping ability for enhancing the semiconductors absorption through light concentration and light scattering, respectively. The 15 nm NPs enhanced the BiVO4 external quantum efficiency throughout the semiconductors absorption range (e.g.,approximate to 2.5 fold at lambda=400 nm). However, when a hole scavenger was added to the electrolyte, no enhancement was ob-served upon NP deposition, indicating that the NPs only facilitate the injection of holes from the semiconductor surface to the electrolyte but do not enhance its absorption. On the other hand, the 65 nm scattering NPs not only facilitated hole injection to the electrolyte, but also enhanced the absorption of the semiconductor (by approximate to 6%) through light scattering. Such a dual effect, i.e., of enhancing both the surface properties and the absorption of the semiconductor, makes light scattering Ag NPs an ideal decoration for PEC water splitting photoelectrodes.

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