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  • 1.
    Liebau, Jobst
    et al.
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Fu, Biao
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Brown, Christian
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Mäler, Lena
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    New insights into the membrane association mechanism of the glycosyltransferase WaaG from Escherichia coli2018In: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1860, no 3, p. 683-690Article in journal (Refereed)
    Abstract [en]

    Monotopic glycosyltransferases (GTs) interact with membranes via electrostatic interactions. The N-terminal domain is permanently anchored to the membrane while the membrane interaction of the C-terminal domain is believed to be weaker so that it undergoes a functionally relevant conformational change upon donor or acceptor binding. Here, we studied the applicability of this model to the glycosyltransferase WaaG. WaaG is involved in the synthesis of lipopolysaccharides (LPS) in Gram-negative bacteria and was previously categorized as a monotopic GT. We analyzed the binding of WaaG to membranes by stopped-flow fluorescence and NMR diffusion experiments. We find that electrostatic interactions are required to bind WaaG to membranes while mere hydrophobic interactions are not sufficient. WaaG senses the membrane's surface charge density but there is no preferential binding to specific anionic lipids. However, the binding is weaker than expected for monotopic GTs but similar to peripheral GTs. Therefore, WaaG may be a peripheral GT and this could be of functional relevance in vivo since LPS synthesis occurs only when WaaG is membrane-bound. We could not observe a C-terminal domain movement under our experimental conditions.

  • 2.
    Liebau, Jobst
    et al.
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Fu, Biao
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Brown, Christian
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    Mäler, Lena
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
    The glycosyltransferase WaaG: a peripheral membrane protein?Manuscript (preprint) (Other academic)
1 - 2 of 2
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