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  • 1. Ballo, Ahmed M.
    et al.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Palmquist, Anders
    Lindahl, Carl
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Emanuelsson, Lena
    Lausmaa, Jukka
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Thomsen, Peter
    Bone tissue reactions to biomimetic ion-substituted apatite surfaces on titanium implants2012In: Journal of the Royal Society Interface, ISSN 1742-5689, E-ISSN 1742-5662, Vol. 9, no 72, p. 1615-1624Article in journal (Refereed)
    Abstract [en]

    The aim of this study was to evaluate the bone tissue response to strontium-and silicon-substituted apatite (Sr-HA and Si-HA) modified titanium (Ti) implants. Sr-HA, Si-HA and HA were grown on thermally oxidized Ti implants by a biomimetic process. Oxidized implants were used as controls. Surface properties, i.e. chemical composition, surface thickness, morphology/pore characteristics, crystal structure and roughness, were characterized with various analytical techniques. The implants were inserted in rat tibiae and block biopsies were prepared for histology, histomorphometry and scanning electron microscopy analysis. Histologically, new bone formed on all implant surfaces. The bone was deposited directly onto the Sr-HA and Si-HA implants without any intervening soft tissue. The statistical analysis showed significant higher amount of bone-implant contact (BIC) for the Si-doped HA modification (P = 0.030), whereas significant higher bone area (BA) for the Sr-doped HA modification (P = 0.034), when compared with the non-doped HA modification. The differences were most pronounced at the early time point. The healing time had a significant impact for both BA and BIC (P < 0.001). The present results show that biomimetically prepared Si-HA and Sr-HA on Ti implants provided bioactivity and promoted early bone formation.

  • 2.
    Ballo, Ahmed
    et al.
    Gothenburg University.
    Omar, Omar
    Gothenburg Univerity.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Palmquist, Anders
    Gothenburg University.
    Dental Implant Surfaces Implant Dentistry - A Rapidly Evolving Practic: Physicochemical Properties, Biological Performance, and Trends2011In: Implant Dentistry: A Rapidly Evolving Practice / [ed] Ilser Turkyilmaz, INTECH , 2011Chapter in book (Refereed)
  • 3.
    Ballo, Ahmed
    et al.
    Gothenburg University.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Lindahl, Carl
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Emanuelsson, Lena
    Gothenburg University.
    Palmqvist, Anders
    Gothenburg University.
    Lausmaa, Jukka
    SP.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Thomsen, Peter
    Gothenburg University.
    Early bone tissue responses to a slicon-substituted apatite/titanium dioxide coating on titanium implant2010Conference paper (Refereed)
  • 4.
    Ballo, Ahmed
    et al.
    Gothenburg University.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Lindahl, Carl
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Lausmaa, Jukka
    SP.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Thomsen, Peter
    Gothenburg University.
    Bone responses to ions-substituted apatite/titanium dioxide coating on titanium implants2010Conference paper (Refereed)
  • 5.
    Berg, Camilla
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Ion substitution induced formation of spherical ceramic particles2019In: Ceramics International, ISSN 0272-8842, E-ISSN 1873-3956, Vol. 45, no 8, p. 10385-10393Article in journal (Refereed)
    Abstract [en]

    How to precipitate ceramic nano- and microspheres in water based solutions only using inorganic ions is a challenge. In this study, spherical particles of alkaline earth phosphates and fluorides were synthesized using a precipitation reaction. Substituting ions, through inhibition of crystal growth, was used to induce sphere formation and to alter the morphology, size and composition of the spheres. The difference in ionic radius between the substituting ion (Mg, Ca and Sr) and the main cation (Sr and Ba) influenced the critical concentration to allow for sphere formation as well as the crystallinity. The larger difference, the lower was the concentration needed to form spheres. Low concentrations of Mg was enough to generate amorphous spheres of Sr- and Ba- phosphates whereas higher concentrations were needed if the radius difference were smaller. An increasing degree of substitution leads to a decrease in crystallinity of precipitated particles. The degree of substitution was determined to 16-55% where a low degree of ion substitution in the phosphates resulted in the formation of spheres (500-800 nm) with rough surfaces composed of apatite like phases. A higher degree of substitution resulted in amorphous spheres (500 nm- (1) mu m) with smooth surfaces.

  • 6.
    Berg, Camilla
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Synthesis of Ion Substituted Ceramic Core-Shell Particles for Dental Applications2018Conference paper (Refereed)
    Abstract [en]

    Calcium phosphate spheres are interesting alternatives for dental applications due to their chemical similarity to teeth and biocompatibility. A spherical shape with a hollow cores allows for loading of therapeutic agents for drug delivery which potentially could be combined with other applications such as tooth remineralization and treatment of hypersensitivity.

    Precipitation reactions are one of the techniques used for synthesizing spherical particles, but little is known about the mechanism behind the sphere formation, which makes tuning of the material properties challenging. Previously, it has been shown that substituting ions can influence the crystallization process, which can enable greater control during the synthesis.In this study, several different substituting ions has been used in the synthesis of alkaline earth phosphates, to further investigate their role in sphere formation and to develop a robust synthesis technique.

    Particles of alkaline earth phosphates (Ca, Sr and Ba) were synthesized with a precipitation reaction. Solutions with constituent anions and cations were mixed at room temperature, and substituting ions (Mg, Ca or Sr) were added before heating at 60-100 °C. Reaction times varied between 10 minutes to 24 hours. Characterization of precipitates was performed with SEM, DLS and FIB to analyze morphology, size and cross-sections of the spheres. Crystal structure and atomic composition was analyzed with XRD and ICP-OES.

    Without substituting ions, precipitates had no specific shape and crystallized in an apatitic structure or as a hydrogenated phosphate. Substituting ions stabilized the initial amorphous phase during the reaction, hindering rapid crystal growth which allowed for self-assembly into hollow, spherical particles with a diameter between 300-700 nm. The phase composition and degree of ion substitution in the precipitates depended on the size and concentration of the substituting ions. The amount of substitution was determined in the range between 5-30 %, where precipitates with a low degree of substitution crystallized in a structure similar to β-tricalcium phosphate, whereas materials with a higher degree of substitution had an amorphous structure.

    In this study it was shown that it was possible to obtain hollow, spherical particles of calcium-, strontium- and barium phosphate, by using substituting ions during a precipitation reaction. This indicates that the approach can be used to tailor the properties of spherical particles intended for dental applications.

  • 7.
    Cai, Bing
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bredenberg, Susanne
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Development and evaluation of self-setting bioceramic microneedles2014Conference paper (Refereed)
  • 8.
    Cai, Bing
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bredenberg, Susanne
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Development and evaluations of self-setting bioceramics microneedles2014Conference paper (Refereed)
  • 9.
    Cai, Bing
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bredenberg, Susanne
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Self-setting bioceramic microscopic protrusions for transdermal drug delivery2014In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 2, no 36, p. 5992-5998Article in journal (Refereed)
    Abstract [en]

    Microneedle (MN) technology offers both an efficient and a minimally invasive transdermal drug delivery strategy. The current MNs, made of silicon and metal, have poor biocompatibility and low drug loading, while the polymer MNs have some constraints related to mechanical strength and storage conditions. In this study, self-setting bioceramics were explored as substitutes for the current MN materials for the first time. Self-setting bioceramic microneedles were fabricated using a master mold by a procedure under mild conditions, which could minimize the drug degradation during fabrication and also facilitates a higher drug loading capability than the other current ceramic microneedles. The drug release and mechanical strength were correlated with the microstructure and porosity of the needles. As observed by SEM and microCT, the ceramic paste could fully fill the geometry of the mould and was cured into an array of micro-sized needles. The drug release study showed that the release rate from this type of MN array could be controlled by the bulk surface area, porosity and resorption rate of the ceramic needles. Applying the MNs to porcine skin indicated that the needles were able to pierce the stratum corneum of the skin. We successfully prepared the bioceramic needles that have high mechanical strength and are resorbable, which can promote safe, efficient and successful transdermal drug delivery.

  • 10.
    Cai, Bing
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bredenberg, Susanne
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Li, Hao
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bioceramic microneedles with flexible and self-swelling substrate2015In: European journal of pharmaceutics and biopharmaceutics, ISSN 0939-6411, E-ISSN 1873-3441, Vol. 94, p. 404-410Article in journal (Refereed)
    Abstract [en]

    To reduce the effort required to penetrate the skin and optimize drug release profiles, bioceramic microneedle arrays with higher-aspect-ratio needles and a flexible and self-swelling substrate have been developed. Swelling of the substrate can assist in separating it from the needles and leave them in the skin as a drug depot. The preparation procedures for this bioceramic microneedle are described in the paper. Clonidine hydrochloride, the model drug, was released in a controlled manner by the microneedle device in vitro. Results showed that the microneedle array with a flexible and self-swelling substrate released the drug content faster than the array with a rigid substrate. Disintegration of the needle material and diffusion of the drug molecules are believed as the main control mechanisms of the drug release from these microneedle arrays. Ex vivo skin penetration showed that they can effectively penetrate the stratum corneum without an extra device. This work represents a progression in the improvement of bioceramic microneedles for transdermal drug delivery.

  • 11.
    Cai, Bing
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bredenberg, Susanne
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Bioceramic microneedles with flexible and self-swelling substrateArticle in journal (Other academic)
  • 12.
    Cai, Yixiao
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Chen, Song
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Grandfield, Kathryn
    McMaster Univ, Dept Mat Sci & Engn, Hamilton, ON, Canada..
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Fabrication of translucent nanoceramics via a simple filtration method2015In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 5, no 121, p. 99848-99855Article in journal (Refereed)
    Abstract [en]

    Generally, particle packing density, grain size and morphology are the important factors that affect the transparency of ceramics. In order to achieve better transparency of ceramics, efforts should be developed to eliminate or minimize light scattering or absorption. Therefore the porosity and size of crystals in a ceramic body should be strictly controlled. Typical transparent ceramics are fabricated by pressure-assisted sintering techniques such as hot isostatic pressing (HIP), spark plasma sintering (SPS), and pressure-less sintering (PLS). However, a simple energy efficient production method remains a challenge. In this study, we describe a simple fabrication process via a facile filtration system that can fabricate translucent hydroxyapatite based ceramics. The translucent pieces yielded from filtration exhibit optical transmittance that was confirmed by UV spectroscopy. Briefly, the morphology and size of ceramic nanoparticles, filtration pressure and filtration time are important parameters to be discussed. Compared with different hydroxyapatite nanoparticles, spherical nanoparticles easily form a densely packed structure, followed by sintered ceramics. When the strontium content in HA increases, the morphology of HA changes from nano-spheres to nano-rods, following a decrease in transparency. A pressure filtration model combining Darcy's law and the Kozeny-Carman relation has been discussed to simulate and explain why the translucent ceramics can be fabricated via such a simple process. This method could be further applied to prepare other translucent functional ceramics by controlling the size and morphology of ceramic particles.

  • 13.
    Cai, Yixiao
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Li, Hu
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Karlsson, Mikael
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Leifer, Klaus
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Biomineralization on Single Crystalline Rutile: The Modulated Growth of Hydroxyapatite by Fibronectin in a Simulated Body Fluid2016In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, p. 35507-35516Article in journal (Refereed)
    Abstract [en]

    The aim of this study is to probe the complex interaction between surface bioactivity and protein adsorption on single crystalline rutile. Our previous studies have shown that single crystalline rutile possessed in vitro bioactivity and the crystalline faces affected the hydroxyapatite (HA) formation. However, upon implantation, a fast adsorption of proteins, from the biological fluids, is intermediated by a water layer towards the biomaterial interface. Thus the effect of protein on the bioactivity must be addressed. In this study, the HA growth dynamics on (001), (100) and (110) faces was investigated in a simulated body fluid with the presence of fibronectin (FN) by two different processes. The surface adhesion of each face before and after FN adsorption, as revealed by direct numerical values, was determined by atomic force microscopy (AFM) based peak force quantitative nanomechanical mapping (PF-QNM) for the first time. The findings suggest the surface energies of FN pre-adsorbed (001), (100) and (110) faces have been enhanced, leading to the subsequent accelerated HA formation. Furthermore, (001) and (100) faces were found to have larger coverage of HA crystals than (110) face at an early stage. In addition, various characterizations were performed to probe the chemical and crystal structures of as-grown biomimetic HA crystals, and in particular, the Ca/P ratio variations at different soaking time points.

  • 14.
    Cai, Yixiao
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Pujari-Palmer, Shiuli
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Gururaj, Satwik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Fu, Le
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Chen, Song
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Karlsson Ott, Marjam
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Utilization of Translucent Hydroxyapatite Nano-Ceramics as a Bio-Window Material2016In: Nano Advances, Vol. 1, p. 45-49Article in journal (Refereed)
    Abstract [en]

    Bioceramic materials are importantlyused in the field ofhard tissue engineering. The direct detection of cell response is almost impossible for mostof bioceramics due to theiropaqueness. Thus,the live tracking of cell behavior cannot be performedon these ceramics. In this study, we proposea strategy thatdirect observation of cell growth through hydroxyapatite (HA)ceramics can be realized by employing a translucent hydroxyapatite (tHA) nano-ceramic. We obtained MC3T3 preosteoblast cells and cultured them in the presence of tHA for up to 7 days. The results show that MC3T3cells were able to be seen through the tHA. In addition, live fluorescent staining confirmed that the MC3T3 cells were viable throughout the culture time period. The findings reveal the as-fabricated tHA nano-ceramics can bepotentialas a bio-window material for cell adhesion and proliferation.

  • 15.
    Cardemil, Carina
    et al.
    University of Gothenburg.
    Elgali, Ibrahim
    University of Gothenburg.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Emanuelsson, Lena
    Gothenburg University.
    Norlindh, Birgitta
    University of Gothenburg.
    Omar, Omar
    University of Gothenburg.
    Thomsen, Peter
    Gothenburg University.
    Strontium-Doped Calcium Phosphate and Hydroxyapatite Granules Promote Different Inflammatory and Bone Remodelling Responses in Normal and Ovariectomised Rats2013In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 12, p. e84932-Article in journal (Refereed)
    Abstract [en]

    The healing of bone defects may be hindered by systemic conditions such as osteoporosis. Calcium phosphates, with or without ion substitutions, may provide advantages for bone augmentation. However, the mechanism of bone formation with these materials is unclear. The aim of this study was to evaluate the healing process in bone defects implanted with hydroxyapatite (HA) or strontium-doped calcium phosphate (SCP) granules, in non-ovariectomised (non-OVX) and ovariectomised (OVX) rats. After 0 (baseline), six and 28d, bone samples were harvested for gene expression analysis, histology and histomorphometry. Tumour necrosis factor-alpha (TNF-alpha), at six days, was higher in the HA, in non-OVX and OVX, whereas interleukin-6 (IL-6), at six and 28d, was higher in SCP, but only in non-OVX. Both materials produced a similar expression of the receptor activator of nuclear factor kappa-B ligand (RANKL). Higher expression of osteoclastic markers, calcitonin receptor (CR) and cathepsin K (CatK), were detected in the HA group, irrespective of non-OVX or OVX. The overall bone formation was comparable between HA and SCP, but with topological differences. The bone area was higher in the defect centre of the HA group, mainly in the OVX, and in the defect periphery of the SCP group, in both non-OVX and OVX. It is concluded that HA and SCP granules result in comparable bone formation in trabecular bone defects. As judged by gene expression and histological analyses, the two materials induced different inflammatory and bone remodelling responses. The modulatory effects are associated with differences in the spatial distribution of the newly formed bone.

  • 16.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Cai, Yixiao
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Enhanced bioactivity of glass ionomer cement by incorporating calcium silicates2016In: Biomatter, ISSN 2159-2527, E-ISSN 2159-2535, Vol. 6, p. e1123842-Article in journal (Refereed)
  • 17.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Cai, Yixiao
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Enhanced bioactivity of glass ionomer cement by incorporating calcium silicates2016In: Biomatter, ISSN 2159-2527, E-ISSN 2159-2535, Vol. 6, no 1, p. e1123842-1-e1123842-13Article in journal (Refereed)
  • 18.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Glass Ionomer Cement Modified by White Portland Cement2014Conference paper (Refereed)
  • 19.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Handling and setting properties of a bioactive glass ionomer cement2015Conference paper (Refereed)
  • 20.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Synthesis and characterization of monetite with thin nacre-like structure2016Conference paper (Refereed)
  • 21.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Two Paste-type Glass Ionomer Cement2014Conference paper (Refereed)
  • 22.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Grandfield, Kathryn
    McMaster University.
    Yu, Shun
    KTH.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Synthesis of calcium phosphate crystals with thin nacreous structure2016In: CrystEngComm, ISSN 1466-8033, E-ISSN 1466-8033, Vol. 18, no 6, p. 1064-1069Article in journal (Refereed)
    Abstract [en]

    Nacre-like structures have attracted great interest in recent years due to their outstanding toughness, stiffness and impact resistance. However, there is a challenge associated with engineering nacre-like calcium phosphate crystals. In this study, thin nacreous-like monetite sheets were synthesized in solutions guided by a surfactant. The influence of temperature, initial pH, Ca/P ratio, stirring time and the concentration of cetyltrimethylammonium bromide (CTAB) on the nacre-like structure has been studied. Findings showed that a nacre-like structure could only be formed at a high temperature (90 degrees C), high initial pH (11), sufficient stirring time (3 h), and under the presence of CTAB. A small-angle X-ray scattering experiment carried out at a synchrotron radiation facility showed that the distance between nanolayers was around 2.6 nm and TEM confirmed the fine sheet-like structure. The mechanism of the formation the nacre-like structure and its characterization were discussed.

  • 23.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Gururaj, Satwik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Synthesis of Ag doped calcium phosphate particles and their antibacterial effect as additives in dental glass ionomer cements2016In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 27, no 11, article id 172Article in journal (Refereed)
    Abstract [en]

    Developing dental restorations with enhanced antibacterial properties has been a constant quest for materials scientists. The aim of this study was to synthesize silver doped calcium phosphate particles and use them to improve antibacterial properties of conventional glass ionomer cement. The Ag doped monetite (Ag-DCPA) and hydroxyapatite (Ag-HA) were synthesized by precipitation method and characterized using X-ray diffraction, scanning electron microscope and X-ray fluorescence spectroscopy. The antibacterial properties of the cements aged for 1 day and 7 days were evaluated by direct contact measurement using staphylococcus epidermis Xen 43. Ion concentrations (F- and Ag+) and pH were measured to correlate to the results of the antibacterial study. The compressive strength of the cements was evaluated with a crosshead speed of 1 mm/min. The glass ionomer cements containing silver doped hydroxyapatite or monetite showed improved antibacterial properties. Addition of silver doped hydroxyapatite or monetite did not change the pH and ion release of F-. Concentration of Ag+ was under the detection limit (0.001 mg/L) for all samples. Silver doped hydroxyapatite or monetite had no effect on the compressive strength of glass ionomer cement.

  • 24.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Mestres, Gemma
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Lan, Weihua
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Cytotoxicity of modified glass ionomer cement on odontoblast cells2016In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 27, no 7, article id 116Article in journal (Refereed)
    Abstract [en]

    Recently a modified glass ionomer cement (GIC) with enhanced bioactivity due to the incorporation of wollastonite or mineral trioxide aggregate (MTA) has been reported. The aim of this study was to evaluate the cytotoxic effect of the modified GIC on odontoblast-like cells. The cytotoxicity of a conventional GIC, wollastonite modified GIC (W-mGIC), MTA modified GIC (M-mGIC) and MTA cement has been evaluated using cement extracts, a culture media modified by the cement. Ion concentration and pH of each material in the culture media were measured and correlated to the results of the cytotoxicity study. Among the four groups, conventional GIC showed the most cytotoxicity effect, followed by W-mGIC and M-mGIC. MTA showed the least toxic effect. GIC showed the lowest pH (6.36) while MTA showed the highest (8.62). In terms of ion concentration, MTA showed the largest Ca2+ concentration (467.3 mg/L) while GIC showed the highest concentration of Si4+ (19.9 mg/L), Al3+ (7.2 mg/L) and Sr2+ (100.3 mg/L). Concentration of F- was under the detection limit (0.02 mg/L) for all samples. However the concentrations of these ions are considered too low to be toxic. Our study showed that the cytotoxicity of conventional GIC can be moderated by incorporating calcium silicate based ceramics. The modified GIC might be promising as novel dental restorative cements.

  • 25.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Mestres, Gemma
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Lan, Weihua
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    In vitro cytotoxicity of dental cements on odontoblast cells2016Conference paper (Refereed)
  • 26.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Pujari-Palmer, Shiuli
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Rubino, Stefano
    Univ Oslo, Dept Phys, Oslo, Norway; Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada.
    Westlund, Viktoria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Ott, Marjam
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Highly repeatable synthesis of nHA with high aspect ratio2015In: Materials letters (General ed.), ISSN 0167-577X, E-ISSN 1873-4979, Vol. 159, p. 163-167Article in journal (Refereed)
  • 27.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Yu, Shun
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Synthesis and characterization of monetite with thin nacreous structure2016Conference paper (Refereed)
  • 28.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Öhman, Caroline
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Jefferies, Steven R.
    Temple Univ, Dept Restorat Dent, Kornberg Sch Dent, Philadelphia, PA 19122 USA.
    Gray, Holly
    Temple Univ, Dept Restorat Dent, Kornberg Sch Dent, Philadelphia, PA 19122 USA.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Compressive fatigue limit of four types of dental restorative materials2016In: Journal of The Mechanical Behavior of Biomedical Materials, ISSN 1751-6161, E-ISSN 1878-0180, Vol. 61, p. 283-289Article in journal (Refereed)
    Abstract [en]

    The purpose of this study was to evaluate the quasi-static compressive strength and the compressive fatigue limit of four different dental restorative materials, before and after aging in distilled water for 30 days. A conventional glass ionomer cement (Fuji IX GP; IG), a zinc-reinforced glass ionomer cement (Chemfil rock; CF), a light curable resin-reinforced glass ionomer cement (Fuji II LC; LC) and a resin-based composite (Quixfil; QF) were investigated. Cylindrical specimens (4 mm in diameter and 6 mm in height) were prepared according to the manufacturer's instructions. The compressive fatigue limit was obtained using the staircase method. Samples were tested in distilled water at 37 degrees C, at a frequency of 10 Hz with 10(5) cycles set as run-out. 17 fatigue samples were tested for each group. Two-way ANOVA and one-way ANOVA followed by Tukey's post-hoc test were used to analyze the results. Among the four types of materials, the resin-based composite exhibited the highest compressive strength (244 +/- 13.0 MPa) and compressive fatigue limit (134 +/- 7.8 MPa), followed by the light-cured resin reinforced glass ionomer cement (168 +/- 8.5 MPa and 92 +/- 6.6 MPa, respectively) after one day of storage in distilled water. After being stored for 30 days, all specimens showed an increase in compressive strength. Aging showed no effect on the compressive fatigue limit of the resin-based composite and the light-cured resin reinforced glass ionomer cement, however, the conventional glass ionomer cements showed a drastic decrease (37% for IG, 31% for CF) in compressive fatigue limit. In conclusion, in the present study, resin modified GIC and resin-based composite were found to have superior mechanical properties to conventional GIC.

  • 29.
    Chen, Song
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Öhman, Caroline
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Jefferies, Steven R.
    Gray, Holly
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Quasi-static compressive strength and compressive fatigue limit of dental cements2016Conference paper (Refereed)
  • 30.
    Cui, Sheng
    et al.
    Nanjing Tech Univ, Coll Mat Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China.;Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct, Nanjing 210009, Jiangsu, Peoples R China..
    Wang, Xue
    Nanjing Tech Univ, Coll Mat Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China.;Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct, Nanjing 210009, Jiangsu, Peoples R China..
    Zhang, Xin
    Nanjing Tech Univ, Coll Mat Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China.;Nanjing Gen Hosp, Dept Neurosurg, Nanjing 210002, Jiangsu, Peoples R China.;Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct, Nanjing 210009, Jiangsu, Peoples R China..
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Tang, Xianglong
    Nanjing Tech Univ, Coll Mat Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China..
    Lin, Benlan
    Nanjing Tech Univ, Coll Mat Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China..
    Wu, Qi
    Nanjing Gen Hosp, Dept Neurosurg, Nanjing 210002, Jiangsu, Peoples R China..
    Shen, Xiaodong
    Nanjing Tech Univ, Coll Mat Sci & Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China.;Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct, Nanjing 210009, Jiangsu, Peoples R China..
    Preparation of magnetic MnFe2O4-Cellulose aerogel composite and its kinetics and thermodynamics of Cu(II) adsorption2018In: Cellulose (London), ISSN 0969-0239, E-ISSN 1572-882X, Vol. 25, no 1, p. 735-751Article in journal (Refereed)
    Abstract [en]

    In this paper, a MnFe2O4-Cellulose magnetic composite aerogel (MnCA) with high adsorption capacity was fabricated by in situ incorporating MnFe2O4 to regenerated cellulose hydrogel matrix, followed by CO2 supercritical drying. A green synthetic strategy was performed by using renewable cellulose materials, environmentally benign cellulose solvents and facile synthetic conditions. The results showed that the obtained magnetic cellulose aerogel had a continuous and tiered three dimensional network with interconnected fibrils of about 30 nm in width, which was similar to those of cellulose aerogel prepared from NaOH/urea solution via CO2 supercritical drying. Meanwhile, they had high specific surface areas of 236-288 m(2)/g and total pore volume of 0.55-0.88 cm(3)/g. In addition, the hybrid aerogel showed superparamagnetism with maximum saturation magnetization reaching up to 18.53 emu/g. The magnetic nanocomposite aerogel could be used for biological and environmental applications. The adsorption test showed that MnCA had rapid adsorption rate and excellent adsorption ability of removing heavy metal ions in aqueous solution which could attain to 63.3 mg/g within 100 min. Moreover, all the composite aerogels exhibited good reusability and could be easily reused from the water after adsorption.

  • 31.
    Elgali, Ibrahim
    et al.
    University of Gothenburg.
    Cardemil, Carina
    University of Gothenburg.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Omar, Omar
    University of Gothenburg.
    Thomsen, Peter
    Gothenburg University.
    Biological Effects of Strontium-Doped Calcium Phosphate and Hydroxyapatite Granules during Bone Healing in Normal and Ovariectomised Rats2013Conference paper (Refereed)
  • 32. Elgali, Ibrahim
    et al.
    Igawa, Kazuyo
    Palmquist, Anders
    Lenneras, Maria
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Choi, Sungjin
    Chung, Ung-Il
    Omar, Omar
    Thomsen, Peter
    Molecular and structural patterns of bone regeneration in surgically created defects containing bone substitutes2014In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 35, no 10, p. 3229-3242Article in journal (Refereed)
    Abstract [en]

    Several biomaterials have been introduced for bone augmentation. However, information is lacking about the mechanisms of bone regeneration and/or integration of these materials in the recipient bone. This study aimed to determine the molecular and structural events in bone defects after augmentation with synthetic tetrapod-shaped calcium phosphate (Tetrabone; TetraB) compared with natural deproteinized bovine bone (DBB). Defects were created in the epiphyses of rat femurs and filled with TetraB or DBB or left empty (Sham). After 3, 6, 14 and 28 d, samples were harvested for histology, histomorphometry, ultrastructure and gene expression analyses. At 3 d, higher expressions of bone formation (ALP and DC) and remodeling (CatK) genes were detected in TetraB compared with DBB and Sham. Downregulation of bone remodeling genes (TRAP and CatK) was detected in DBB as compared to Sham after 14 d. Histomorphometry at 6 and 14 d demonstrated greater bone contact with the granules in TetraB. At 28 d, a larger bone area per defect was found in TetraB. The present experiments show that a synthetic substitute, consisting of alpha-tricalcium and octacalcium phosphates, induces early osteogenic and osteoclastic activities and promotes bone formation in trabecular bone defects.

  • 33.
    Elgali, Ibrahim
    et al.
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden..
    Turri, Alberto
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden.;Publ Dent Hlth Care, Inst Odontol, Branemark Clin, Gothenburg, Sweden..
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Norlindh, Birgitta
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden..
    Johansson, Anna
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden..
    Dahlin, Christer
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden.;NU Hosp Org, Dept Oral Maxillofacial Surg & Res & Dev, Trollhattan, Sweden..
    Thomsen, Peter
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden..
    Omar, Omar
    BIOMATCELL VINN Excellence Ctr Biomat & Cell Ther, Gothenburg, Sweden.;Univ Gothenburg, Dept Biomat, Inst Clin Sci, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden..
    Guided bone regeneration using resorbable membrane and different bone substitutes: Early histological and molecular events2016In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 29, p. 409-423Article in journal (Refereed)
    Abstract [en]

    Bone insufficiency remains a major challenge for bone-anchored implants. The combination of guided bone regeneration (GBR) and bone augmentation is an established procedure to restore the bone. However, a proper understanding of the interactions between the bone substitute and GBR membrane materials and the bone-healing environment is lacking. This study aimed to investigate the early events of bone healing and the cellular activities in response to a combination of GBR membrane and different calcium phosphate (CaP) materials. Defects were created in the trabecular region of rat femurs, and filled with deproteinized bovine bone (DBB), hydroxyapatite (HA) or strontium-doped HA (SrHA) or left empty (sham). All the defects were covered with an extracellular matrix membrane. Defects were harvested after 12 h, 3 d and 6 d for histology/histomorphometry, immunohistochemistry and gene expression analyses. Histology revealed new bone, at 6 d, in all the defects. Larger amount of bone was observed in the SrHA-filled defect. This was in parallel with the reduced expression of osteoclastic genes (CR and CatK) and the osteoblast-osteoclast coupling gene (RANKL) in the SrHA defects. Immunohistochemistry indicated fewer osteoclasts in the SrHA defects. The observations of CD68 and periostin-expressing cells in the membrane per se indicated that the membrane may contribute to the healing process in the defect. It is concluded that the bone-promoting effects of Sr in vivo are mediated by a reduction in catabolic and osteoblast-osteoclast coupling processes. The combination of a bioactive membrane and CaP bone substitute material doped with Sr may produce early synergistic effects during GBR. Statement of significance The study provides novel molecular, cellular and structural evidence on the promotion of early bone regeneration in response to synthetic strontium-containing hydroxyapatite (SrHA) substitute, in combination with a resorbable, guided bone regeneration (GBR) membrane. The prevailing view, based mainly upon in vitro data, is that the beneficial effects of Sr are exerted by the stimulation of bone-forming cells (osteoblasts) and the inhibition of bone-resorbing cells (osteoclasts). In contrast, the present study demonstrates that the local effect of Sr in vivo is predominantly via the inhibition of osteoclast number and activity and the reduction of osteoblast-osteoclast coupling. This experimental data will form the basis for clinical studies, using this material as an interesting bone substitute for guided bone regeneration.

  • 34.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Andersson, Martin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Unosson, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Influence of alkali metal additives on phase transition of translucent ZrO2/SiO2 glass ceramics prepared by a sol-gel method2015In: European Cells and Materials, ISSN 1473-2262, E-ISSN 1473-2262, Vol. 29Article in journal (Refereed)
  • 35.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Andersson, Martin
    Unosson, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Persson, Cecilia
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Influence of alkali metal additives on phase transition of translucent ZrO2/SiO2 glass ceramics prepared by a sol-gel method2015In: European Cells and Materials, ISSN 1473-2262, E-ISSN 1473-2262, Vol. 29, no S1, p. 10-Article in journal (Refereed)
  • 36.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Andersson, Martin
    Unosson, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Persson, Cecilia
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Influences of alkali metals additives on phase transition of translucent ZrO2/SiO2 glass ceramic prepared by sol-gel method2015Conference paper (Refereed)
  • 37.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Highly translucent and strong ZrO2-SiO2 nanocrystalline glass ceramic prepared by sol-gel method and spark plasma sintering with fine 3D microstructure for dental restoration2017In: Journal of the European Ceramic Society, ISSN 0955-2219, E-ISSN 1873-619X, Vol. 37, no 13, p. 4067-4081Article in journal (Refereed)
    Abstract [en]

    Balance of better mechanical strength and good translucency for dental restorative materials is always a challenge. A translucent glass ceramic/ceramic with improved mechanical properties or a strong glass ceramic/ceramic with good translucency would therefore be interesting for dental application. Nanocrystalline glass ceramics (NCGC) attract a lot attention because of their superior optical and mechanical properties. This study aims to obtain ZrO2-SiO2 nanocrystalline glass-ceramic that possesses high mechanical strength as well as excellent translucency by controlling the content, size, and connection of nanocrystalline ZrO2 in a ZrO2-SiO2 glass-ceramic material. Toward this end, well-homogenized nano powders with three different compositions, 45%ZrO2-55%SiO2 (molar ratio, 45Zr), 55%ZrO2-45%SiO2 (55Zr), and 65%ZrO2-35%SiO2 (65Zr), were synthesized, followed by a fast sintering process. Highly translucent nanocrystalline glass ceramics composed of tetragonal ZrO2 were obtained. Samples with high zirconia content showed that the structure of the skeleton was predominately built by nano-sized ellipsoidal ZrO2 particles bonded by grain boundaries, with amorphous SiO2 filling the voids between the ZrO2 particles. The achieved flexural strength measured by piston-on-three-ball test was as high as 1014 MPa. To our knowledge, this is one of the highest flexural strength values of glass ceramics ever reported, which is higher than transparent zirconia and alumina ceramics. The 3D structure of nanocrystalline zirconia in silica matrix did enhance the flexural strength of the NCGC. The results of this study suggest that the new ZrO2-SiO2 NCGC has great potential of using as dental restoration.

  • 38.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Spark plasma sintering of biodegradable Si3N4 bioceramic with Sr, Mg and Si as sintering additives for spinal fusion2018In: Journal of the European Ceramic Society, ISSN 0955-2219, E-ISSN 1873-619X, Vol. 38, no 4, p. 2110-2119Article in journal (Refereed)
    Abstract [en]

    Silicon nitride (Si3N4) bioceramics with standard sintering additives (Al2O3 and Y2O3) are used in spinal fusion. Alternative Si3N4 bioceramics with biologically beneficial sintering additives could lead to improved osseoin- tegrative properties. The aim of this study is to obtain dense and strong Si3N4 bioceramics by using SrO, MgO and SiO2 as sintering additives, and evaluate the effect of these sintering additives on microstructures and properties of Si3N4 bioceramics. Raw powders with 10 wt% and 18 wt% sintering additives were sintered by spark plasma sintering. Samples sintered at 1750 °C, with an applied pressure of 60 MPa and a holding time of 3 min, showed the highest content of β-Si3N4 (94.9%). The mechanical properties of the developed Si3N4 bio- ceramics are comparable to the mechanical properties of currently used structural Si3N4 ceramics sintered with standard sintering additives (Al2O3 and Y2O3). The highest flexural strength of the developed Si3N4 bioceramics reached 1079 MPa. Ion release results showed that Sr2+,Mg2+ and Si4+ ions kept leaching out within 10 days’ immersion. The degradable Si3N4 bioceramics with adequate strength and biologically beneficial sintering ad- ditives show the promise for load bearing biomedical applications, such as spinal fusion.

  • 39.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Unosson, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Wu, Chengtie
    Grandfield, Kathryn
    McMaster University.
    Chang, Jiang
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Spark plasma sintering consolidated transparent ZrO2- SiO2 glass ceramic containing nano-sized ZrO2 spheres2016Conference paper (Refereed)
  • 40.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Wu, Chengtie
    Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, 1295 Dingxi Rd, Shanghai 200050, Peoples R China..
    Grandfield, Kathryn
    McMaster Univ, Dept Mat Sci & Engn, Hamilton, ON, Canada.;McMaster Univ, Sch Biomed Engn, Hamilton, ON, Canada..
    Unosson, Erik
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Chang, Jiang
    Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, 1295 Dingxi Rd, Shanghai 200050, Peoples R China..
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Transparent single crystalline ZrO2-SiO2 glass nanoceramic sintered by SPS2016In: Journal of the European Ceramic Society, ISSN 0955-2219, E-ISSN 1873-619X, Vol. 36, no 14, p. 3487-3494Article in journal (Refereed)
    Abstract [en]

    Transparent ZrO2-SiO2 glass ceramics show potential for application in the dental industry. The application of this material prepared by a sol-gel method was hindered by the difficulties in obtaining large dimension samples. Spark plasma sintering (SPS) offers the possibility of achieving transparent ZrO2-SiO2 glass ceramics. In this study, a ZrO2-SiO2 powder was prepared by the sol-gel method and subsequently sintered by SPS. Varied sintering temperatures and pressures were explored to achieve better mechanical strength and transparency. TEM results showed single crystalline ZrO2 spherical nanocrystals (approximately 20 nm) homogenously embedded in the SiO2 matrix. Tetragonal ZrO2 was the only crystalline phase in all specimens. With sintering conditions of 1200 degrees C and 30 MPa, a glass ceramic with fracture toughness of 4.13 MPa was obtained. This value is similar to the commercial dental glass ceramic of IPS e.max (R) Press. The studied transparent glass ceramic with high transparency and moderate mechanical strength shows promise for dental application.

  • 41.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xie, Ling
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Fu, Wenbo
    Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianshan Road 64, Mianyang, Sichuan 621900, People’s Republic of China.
    Hu, Shuanglin
    Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianshan Road 64, Mianyang, Sichuan 621900, People’s Republic of China.
    Zhang, Zhibin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
    Leifer, Klaus
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Ultrastrong translucent glass ceramic with nanocrystalline, biomimetic structure2018In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 18, no 11, p. 7146-7154Article in journal (Refereed)
    Abstract [en]

    Transparent/translucent glass ceramics (GCs) have broad applications in biomedicine, armor, energy, and constructions. However, GCs with improved optical properties typically suffer from impaired mechanical properties, compared to traditional sintered full-ceramics. We present a method of obtaining high-strength, translucent GCs by preparing ZrO2-SiO2 nanocrystalline glass ceramics (NCGCs), with a microstructure of monocrystalline ZrO2 nanoparticles (NPs), embedded in an amorphous SiO2 matrix. The ZrO2-SiO2 NCGC with a composition of 65%ZrO2-35%SiO2 (molar ratio, 65Zr) achieved an average flexural strength of 1 GPa. This is one of the highest flexural strength values ever reported for GCs. ZrO2 NPs have a core-shell structure, and the shell is a thin (2–3 nm) amorphous Zr/Si interfacial layer that provides strong bonding between the ZrO2 NPs and SiO2 matrix. The diffusion of Si atoms into the ZrO2 NPs forms a Zr-O-Si superlattice. Electron tomography results show that some of the ZrO2 NPs are connected in one direction, forming in situ ZrO2 nanofibers (with length of ~500 nm), and that the ZrO2 nanofibers are stacked in an ordered way in all three dimensions. The nano-architecture of the ZrO2 nanofibers mimics the architecture of mineralized collagen fibril in cortical bone. Strong interface bonding enables efficient load transfer from the SiO2 matrix to the 3D nano-architecture built by ZrO2 nanofibers and NPs, and the 3D nano-architecture carries the majority of the external load. These two factors synergistically contribute to the high strength of the 65Zr NCGC. This study deepens our fundamental understanding of the microstructure-mechanical strength relationship, which could guide the design and manufacture of other high-strength, translucent GCs.

  • 42.
    Fu, Le
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xiong, Yi
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Carlsson, Gunnar
    Swedish University of Agricultural Sciences.
    Palmer, Michael
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Örn, Stefan
    Swedish University of Agricultural Sciences.
    Zhu, Wei
    Chinese Academy of Medical Sciences & Peking Union Medical College.
    Xisheng, Weng
    Chinese Academy of Medical Sciences & Peking Union Medical College.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Biodegradable Si3N4 bioceramic sintered with Sr, Mg and Si for spinal fusion: Surface characterization and biological evaluation2018In: Applied Materials Today, ISSN 2352-9407, Vol. 12, p. 260-275Article in journal (Refereed)
    Abstract [en]

    Silicon nitride (Si3N4) is an industrial ceramic used in spinal fusion and maxillofacial reconstructionbecause of its excellent mechanical properties and good biocompatibility. This study compares the sur-face properties, apatite formation ability, bacterial infection, cell-biomaterial interactions, and in vivotoxicity (zebrafish) of newly developed Si3N4 bioceramics (sintered with bioactive sintering additivesSrO, MgO and SiO2) with two standard biomaterials; titanium (Ti) and traditional Si3N4 bioceramics (sin-tered with standard sintering additives Al2O3 and Y2O3). In general, Si3N4 bioceramics (both the newlydeveloped and the traditional) displayed less in vitro bacterial affinity than Ti, which may arise fromdifferences in the surface properties between these two types of material. The newly developed Si3N4bioceramics developed lower biofilm coverage and thinner biofilm, compared to traditional Si3N4 bioce-ramics. The effects of ionic dissolution products (leach) on proliferation and differentiation of MC3T3-E1cell were also investigated. Ionic dissolution products containing moderate amount of Sr, Mg and Siions (approximately 4.72 mg/L, 3.26 mg/L and 3.67 mg/L, respectively) stimulated osteoblast prolifera-tion during the first 2 days in culture. Interestingly, ionic dissolution products from the traditional Si3N4bioceramics that contained small amount of Si and Y ions achieved the greatest stimulatory effect foralkaline phosphatase activity after 7 days culture. The toxicity of ionic dissolution products was investi-gated in a putative developmental biology model: zebrafish (Danio rerio). No toxicity, or developmentalabnormalities, was observed in zebrafish embryos exposed to ionic dissolution products, for up to 144 hpost fertilization. These newly developed Si3N4 bioceramics with bioactive sintering additives show greatpotential as orthopedic implants, for applications such as spinal fusion cages. Future work will focus onevaluation of the newly developed Si3N4 bioceramics using a large animal model.

  • 43.
    Garbani, M.
    et al.
    Univ Zurich, Swiss Inst Allergy & Asthma Res, Davos, Switzerland..
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Rhyner, C.
    Univ Zurich, Swiss Inst Allergy & Asthma Res, Davos, Switzerland..
    Prati, M.
    Univ Zurich, Swiss Inst Allergy & Asthma Res, Davos, Switzerland..
    Scheynius, A.
    Karolinska Inst, Stockholm, Sweden.;Univ Hosp, Dept Med Solna, Translat Immunol Unit, Stockholm, Sweden..
    Malissen, B.
    Ctr Immunol Marseille Luminy, Marseille, France..
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Maurer, M.
    Charite, D-13353 Berlin, Germany..
    Crameri, R.
    Univ Zurich, Swiss Inst Allergy & Asthma Res, Davos, Switzerland..
    Terhorst, D.
    Ctr Immunol Marseille Luminy, Marseille, France.;Charite, D-13353 Berlin, Germany..
    Novel microparticles create a slow releasing depot for long-term immunostimulation in allergen-specific immunotherapy2016In: Experimental dermatology, ISSN 0906-6705, E-ISSN 1600-0625, Vol. 25, no 3, p. E20-E21Article in journal (Refereed)
  • 44.
    Garbani, M.
    et al.
    Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Rhyner, C.
    Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich.
    Prati, M.
    Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich.
    Scheynius, Annika
    Karolinska Inst, Dept Clin Sci & Educ, Stockholm, Sweden.; Soder Sjukhuset, Sachs Children & Youth Hosp, Stockholm, Sweden.
    Malissen, B.
    Centre d’Immunologie de Marseille-Luminy (CIML), INSERM.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Maurer, M.
    Department of Dermatology and Allergy, Charité -Universitätsmedizin Berlin,.
    Crameri, Reto
    Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich,.
    Terhorst-Molawi, Dorothea
    UM2 Aix Marseille Univ, INSERM, U1104, CNRS,UMR7280,CIML, Marseille 9, France.; Charite, Dept Dermatol & Allergy, Allergie Ctr Charite, Berlin, Germany.; BIH, Berlin, Germany.
    Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice2017In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 72, no 4, p. 570-578Article in journal (Refereed)
    Abstract [en]

    Background

    Immunomodulatory interventions play a key role in the treatment of infections and cancer as well as allergic diseases. Adjuvants such as micro- and nanoparticles are often added to immunomodulatory therapies to enhance the triggered immune response. Here, we report the immunological assessment of novel and economically manufactured microparticle adjuvants, namely strontium-doped hydroxyapatite porous spheres (SHAS), which we suggest for the use as adjuvant and carrier in allergen-specific immunotherapy (ASIT).

    Methods and Results

    Scanning electron microscopy revealed that the synthesis procedure developed for the production of SHAS results in a highly homogeneous population of spheres. SHAS bound and released proteins such as ovalbumin (OVA) or the major cat allergen Fel d 1. SHAS-OVA were taken up by human monocyte-derived dendritic cells (mdDCs) and murine DCs and did not have any necrotic or apoptotic effects even at high densities. In a murine model of ASIT for allergic asthmatic inflammation we found that OVA released from subcutaneously injected SHAS-OVA led to a sustained stimulation of both CD4+ and CD8+ T-cells. ASIT with SHAS-OVA as compared to soluble OVA resulted in similar humoral responses but in a higher efficacy as assessed by symptom scoring.

    Conclusion

    We conclude that SHAS may constitute a suitable carrier and adjuvant for ASIT with great potential due to its unique protein-binding properties.

  • 45.
    Gou, Zhongru
    et al.
    Zhejiang University.
    Ballo, Ahmed
    Gothenburg University.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Biomimetic preparation and biomineralization of bioceramics2012In: Advanced bioactive inorganic materials for bone regeneration and drug delivery, CRC Press and Taylor & Francis , 2012Chapter in book (Refereed)
  • 46.
    Grandfield, Kathryn
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Pujari, Shiuli
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Ott, Marjam
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Effect of Calcium and Strontium on Mesoporous Titania Coatings for Implant Applications2013In: Journal of Biomaterials and Nanobiotechnology, ISSN 2158-7027, E-ISSN 2158-7043, Vol. 4, no 2, p. 107-113Article in journal (Refereed)
    Abstract [en]

    ncreasing interest in the role of ions such as calcium and strontium in bone formation has called for the investigation of multifunctional ion-doped implant coatings. Mesoporous titania coatings incorporating calcium or strontium enabled a unique pore morphology and potential for drug delivery. Coatings were produced on titanium by an evaporation induced self-assembly method with the addition of calcium or strontium to the sol causing a shift in morphology from a hexagonally-packed to a worm-like porous network. Pore sizes ranged from 3.8 - 5 nm and coatings exhibited high surface areas between 181 - 215.5 m2/g, as measured by N2adsorption-desorption. Coatings were loaded with 1 mg/ml Cephalothin, and showed sustained release of the antibiotic over one week in vitro. Cell studies confirmed that the ion addition had no toxic effect on human-like osteoblastic SaOS-2 cells. The results of this study suggest the potential for mesoporous coatings with calcium or strontium incorporation for direct bone-interfacing and combined drug delivery implant applications.

  • 47.
    Grandfield, Kathryn
    et al.
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Pujari, Shiuli
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Ott, Marjam
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Mesoporous titania implant coatings with and without calcium and strontium ion incorporation2012Conference paper (Refereed)
  • 48.
    Hu, Zhekai
    et al.
    Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral & Craniomaxillofacial Surg,Sch Med, Shanghai Key Lab Stomatol,Shanghai Res Inst Stoma, Shanghai 200011, Peoples R China.
    Wang, Xiuhui
    Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Wang, Zongliang
    Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Jilin, Peoples R China.
    Zhang, Peibiao
    Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Jilin, Peoples R China.
    Xia, Lunguo
    Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthodont, Collage Stomatol,Sch Med, Shanghai 200011, Peoples R China.
    Lin, Kaili
    Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral & Craniomaxillofacial Surg,Sch Med, Shanghai Key Lab Stomatol,Shanghai Res Inst Stoma, Shanghai 200011, Peoples R China.
    Zhu, Min
    Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral & Craniomaxillofacial Surg,Sch Med, Shanghai Key Lab Stomatol,Shanghai Res Inst Stoma, Shanghai 200011, Peoples R China.
    Nano-Structure Designing Promotion Osseointegration of Hydroxyapatite Coated Ti-6Al-4V Alloy Implants in Diabetic Model2019In: Journal of Biomedical Nanotechnology, ISSN 1550-7033, E-ISSN 1550-7041, Vol. 15, no 8, p. 1701-1713Article in journal (Refereed)
    Abstract [en]

    Mammalian diabetes mellitus which contains altered microenvironment always impairs diverse cellular processes such as osteogenesis, angiogenesis and tissue regeneration via different mechanisms. For researches in materials science, modifying the ability of osteogenesis and angiogenesis in dental implants shows its significant importance. Nano-structure designing is considered as a facile strategy to improve the surface bioactivity of the implants. In this study, the nanorod-structured hydroxyapatite (HA) coatings on Ti-6Al-4V implants were facilely designed by the combination of plasma-spraying and hydrothermal treatment via varying reaction media. Intriguingly, hydrothermal treatment eliminated the glassy phase and impurity phases of HA coatings, and nanorod-structured surface was successfully constructed under hydrothermal treatment in Na3PO4 solution. Additionally, the HA coatings with nanorod-structured surface effectively promoted the adhesion and proliferation and further enhanced osteogenic differentiation of DM-rBMSCs in vitro, Moreover, the osseointegration of Ti-6Al-4V implants with nanorod-structured HA coating was also enhanced in diabetes mellitus rabbit model in vivo. Therefore, the nano-structured surface modification of HA coating on Ti-6Al-4V implants could target pathological bone loss via strengthening osteogenesis and angiogenesis and further potentially used as a therapeutic coating to promote diabetic osteointegration.

  • 49.
    Hulsart-Billström, Gry
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics.
    Carlsson, Elin
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Larsson, Sune
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    In vivo and in vitro performance of Sr-doped hydroxyapatite composite in the form of hollow nano-spheres2012Conference paper (Refereed)
  • 50.
    Hulsart-Billström, Gry
    et al.
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics.
    Xia, Wei
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Pankotai, Eszter
    Department of Orthopedics, Semmelweis University, Budapest, Ungern.
    Weszl, Miklos
    Department of Orthopedics, Semmelweis University, Budapest, Ungern.
    Carlsson, Erik
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics.
    Engqvist, Håkan
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
    Lacza, Zsombor
    Department of Orthopedics, Semmelweis University, Budapest, Ungern.
    Larsson, Sune
    Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Orthopaedics.
    Bone forming potential of Sr doped hydroxyapatite hollow spheres in a rat vertebral bone defect model2012In: Bone, ISSN 8756-3282, E-ISSN 1873-2763, Vol. 50, no supplement 1, p. S114-Article in journal (Refereed)
123 1 - 50 of 136
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