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  • 1.
    Sepehri, Sobhan
    et al.
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden.
    Agnarsson, Björn
    Chalmers Univ Technol, Dept Phys, Gothenburg, Sweden.
    Zardán Gómez de la Torre, Teresa
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
    Schneiderman, Justin F.
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden; Univ Gothenburg, MedTech West, Gothenburg, Sweden; Univ Gothenburg, Inst Neurosci & Physiol, Gothenburg, Sweden.
    Blomgren, Jakob
    RISE Res Inst Sweden, Gothenburg, Sweden.
    Jesorka, Aldo
    Johansson, Christer
    RISE Res Inst Sweden, Gothenburg, Sweden.
    Nilsson, Mats
    Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, Solna, Sweden.
    Albert, Jan
    Karolinska Univ Hosp, Dept Clin Microbiol, Stockholm, Sweden; Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
    Strömme, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
    Winkler, Dag
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden.
    Kalaboukhov, Alexei
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden.
    Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay2019In: Biosensors, ISSN 2079-6374, Vol. 9, no 3, article id 109Article in journal (Refereed)
    Abstract [en]

    The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.

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