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  • 1. Sepehri, Sobhan
    et al.
    Agnarsson, Björn
    de la Torre, Teresa Zardán Gómez
    Schneiderman, Justin E.
    Blomgren, Jakob
    Jesorka, Aldo
    Johansson, Christer
    Nilsson, Mats
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
    Albert, Jan
    Strømme, Maria
    Winkler, Dag
    Kalaboukhov, Alexei
    Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay2019In: Biosensors, ISSN 2079-6374, Vol. 9, no 3, article id 109Article in journal (Refereed)
    Abstract [en]

    The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.

  • 2.
    Sepehri, Sobhan
    et al.
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden.
    Agnarsson, Björn
    Chalmers Univ Technol, Dept Phys, Gothenburg, Sweden.
    Zardán Gómez de la Torre, Teresa
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
    Schneiderman, Justin F.
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden; Univ Gothenburg, MedTech West, Gothenburg, Sweden; Univ Gothenburg, Inst Neurosci & Physiol, Gothenburg, Sweden.
    Blomgren, Jakob
    RISE Res Inst Sweden, Gothenburg, Sweden.
    Jesorka, Aldo
    Johansson, Christer
    RISE Res Inst Sweden, Gothenburg, Sweden.
    Nilsson, Mats
    Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, Solna, Sweden.
    Albert, Jan
    Karolinska Univ Hosp, Dept Clin Microbiol, Stockholm, Sweden; Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
    Strömme, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
    Winkler, Dag
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden.
    Kalaboukhov, Alexei
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden.
    Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay2019In: Biosensors, ISSN 2079-6374, Vol. 9, no 3, article id 109Article in journal (Refereed)
    Abstract [en]

    The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.

  • 3. Sepehri, Sobhan
    et al.
    de la Torre, Teresa Zardán Gomez
    Schneiderman, Justin F.
    Blomgren, Jakob
    Jesorka, Aldo
    Johansson, Christer
    Nilsson, Mats
    Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
    Albert, Jan
    Strømme, Maria
    Winkler, Dag
    Kalaboukhovf, Alexei
    Homogeneous Differential Magnetic Assay2019In: Acs Sensors, ISSN 2379-3694, Vol. 4, no 9, p. 2381-2388Article in journal (Refereed)
    Abstract [en]

    Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

  • 4.
    Sepehri, Sobhan
    et al.
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden.
    Zardán Gómez de la Torre, Teresa
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
    Schneiderman, Justin F.
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden;Univ Gothenburg, MedTech West, SE-40530 Gothenburg, Sweden;Univ Gothenburg, Inst Neurosci & Physiol, SE-40530 Gothenburg, Sweden.
    Blomgren, Jakob
    RISE Res Inst Sweden, SE-41133 Gothenburg, Sweden.
    Jesorka, Aldo
    Chalmers Univ Technol, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden.
    Johansson, Christer
    RISE Res Inst Sweden, SE-41133 Gothenburg, Sweden.
    Nilsson, Mats
    Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Box 1031, SE-17121 Solna, Sweden.
    Albert, Jan
    Karolinska Univ Hosp, Dept Clin Microbiol, SE-17176 Stockholm, Sweden;Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden.
    Strömme, Maria
    Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
    Winkler, Dag
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden.
    Kalaboukhovf, Alexei
    Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden.
    Homogeneous Differential Magnetic Assay2019In: ACS SENSORS, ISSN 2379-3694, Vol. 4, no 9, p. 2381-2388Article in journal (Refereed)
    Abstract [en]

    Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

1 - 4 of 4
CiteExportLink to result list
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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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  • text
  • asciidoc
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