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  • 1.
    Abellan-Flos, Marta
    et al.
    Univ Namur, Dept Chim, Lab Chim Bioorgan, Rue Bruxelles 61, B-5000 Namur, Belgium.;PSL Univ, CNRS, ESPCI Paris, Mol Macromol Chem & Mat, 10 Rue Vauquelin, F-75005 Paris, France..
    Timmer, Brian J. J.
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi.
    Altun, Samuel
    Attana AB, Bjornnasvagen 21, S-11419 Stockholm, Sweden..
    Aastrup, Teodor
    Attana AB, Bjornnasvagen 21, S-11419 Stockholm, Sweden..
    Vincent, Stephane P.
    Univ Namur, Dept Chim, Lab Chim Bioorgan, Rue Bruxelles 61, B-5000 Namur, Belgium..
    Ramström, Olof
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi. Univ Massachusetts, Dept Chem, One Univ Ave, Lowell, MA 01854 USA.;Linnaeus Univ, Dept Chem & Biomed Sci, SE-39182 Kalmar, Sweden..
    QCM sensing of multivalent interactions between lectins and well-defined glycosylated nanoplatforms2019Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 139, artikkel-id 111328Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Quartz crystal microbalance (QCM) methodology has been adopted to unravel important factors contributing to the "cluster glycoside effect" observed in carbohydrate-lectin interactions. Well-defined, glycosylated nanostructures of precise sizes, geometries and functionalization patterns were designed and synthesized, and applied to analysis of the interaction kinetics and thermodynamics with immobilized lectins. The nanostructures were based on Borromean rings, dodecaamine cages, and fullerenes, each of which carrying a defined number of carbohydrate ligands at precise locations. The synthesis of the Borromeates and dodecaamine cages was easily adjustable due to the modular assembly of the structures, resulting in variations in presentation mode. The binding properties of the glycosylated nanoplatforms were evaluated using flow-through QCM technology, as well as hemagglutination inhibition assays, and compared with dodecaglycosylated fullerenes and a monovalent reference. With the QCM setup, the association and dissociation rate constants and the associated equilibrium constants of the interactions could be estimated, and the results used to delineate the multivalency effects of the lectin-nanostructure interactions.

  • 2.
    Abellan-Flos, Marta
    et al.
    Univ Namur, Belgium;PSL Univ, France.
    Timmer, Brian J. J.
    KTH Royal Instute of Technology, Sweden.
    Altun, Samuel
    Attana AB, Sweden.
    Aastrup, Teodor
    Attana AB, Sweden.
    Vincent, Stephane P.
    Univ Namur, Belgium.
    Ramström, Olof
    Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). KTH Royal Instute of Technology, Sweden;Univ Massachusetts, USA.
    QCM sensing of multivalent interactions between lectins and well-defined glycosylated nanoplatforms2019Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 139, artikkel-id 111328Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Quartz crystal microbalance (QCM) methodology has been adopted to unravel important factors contributing to the "cluster glycoside effect" observed in carbohydrate-lectin interactions. Well-defined, glycosylated nanostructures of precise sizes, geometries and functionalization patterns were designed and synthesized, and applied to analysis of the interaction kinetics and thermodynamics with immobilized lectins. The nanostructures were based on Borromean rings, dodecaamine cages, and fullerenes, each of which carrying a defined number of carbohydrate ligands at precise locations. The synthesis of the Borromeates and dodecaamine cages was easily adjustable due to the modular assembly of the structures, resulting in variations in presentation mode. The binding properties of the glycosylated nanoplatforms were evaluated using flow-through QCM technology, as well as hemagglutination inhibition assays, and compared with dodecaglycosylated fullerenes and a monovalent reference. With the QCM setup, the association and dissociation rate constants and the associated equilibrium constants of the interactions could be estimated, and the results used to delineate the multivalency effects of the lectin-nanostructure interactions.

  • 3. Afshar, Majid Ghahraman
    et al.
    Crespo, Gaston A.
    Bakker, Eric
    Counter electrode based on an ion-exchanger Donnan exclusion membrane for bioelectroanalysis2014Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 61, s. 64-69Artikkel i tidsskrift (Fagfellevurdert)
  • 4. Albrecht, Christiane
    et al.
    Fechner, Peter
    Honcharenko, Dmytro
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för biokemi och organisk kemi.
    Baltzer, Lars
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för biokemi och organisk kemi.
    Gauglitz, Günther
    A new assay design for clinical diagnostics based on alternative recognition elements2010Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 25, nr 10, s. 2302-2308Artikkel i tidsskrift (Fagfellevurdert)
  • 5.
    Alcock, S. J.
    et al.
    Cranfield university, UK.
    Danielsson, B.
    Cranfield university, UK.
    Turner, Anthony P. F.
    Cranfield University, UK.
    Advances in the use of  in vivo sensors1992Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 7, nr 4, s. 243-254Artikkel i tidsskrift (Annet vitenskapelig)
    Abstract [en]

    The sixth workshop of the European Community Concerted Action on Chemical sensors for in vivo monitoring was held at Snogeholm, Sweden, in October 1991. The meeting reviewed recent in vivo and ex vivo results, and also included a shorter session on ethical and safety problems.

  • 6.
    ALCOCK, SJ
    et al.
    UNIV IOANNINA,DEPT CHEM,IOANNINA,GREECE; .
    KARAYANNIS, M
    UNIV IOANNINA,DEPT CHEM,IOANNINA,GREECE; .
    TURNER, APF
    Cranfield University, UK.
    THE DESIGN AND DEVELOPMENT OF NEW CHEMICAL SENSORS FOR INVIVO MONITORING1991Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 6, nr 8, s. 647-652Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The latest workshop of the European Community (EC) Concerted Action on Chemical sensors for in vivo monitoring was held in Nauplion, Greece, in April this year. This fifth workshop focused on The design and development of new sensors for in vivo monitoring, and was organized into five sessions: design and development of new sensors; operational considerations; performance of analytical systems; novel sensors/tissue heterogeneity; and infra-red spectroscopy.

  • 7. Ampurdanes, Jordi
    et al.
    Crespo, Gaston A.
    Maroto, Alicia
    Angeles Sarmentero, M.
    Ballester, Pablo
    Xavier Rius, F.
    Determination of choline and derivatives with a solid-contact ion-selective electrode based on octaamide cavitand and carbon nanotubes2009Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 25, nr 2, s. 344-349Artikkel i tidsskrift (Fagfellevurdert)
  • 8.
    Anderson, Tony
    et al.
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för medicin och vård, Farmakologi.
    Filippini, Daniel
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
    Suska, Anke
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Johansson, Therese
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för medicin och vård, Farmakologi.
    Svensson, Samuel
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för medicin och vård, Farmakologi.
    Lundström, Ingemar
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
    Frog melanophores cultured on fluorescent microbeads: Biomimic-based biosensing2005Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 21, nr 1, s. 111-120Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Melanophores are pigmented cells in lower vertebrates capable of quick color changes and thereby suitable as whole cell biosensors. In the frog dermis skin layer, the large and dark pigmented melanophore surrounds a core of other pigmented cells. Upon hormonal stimulation the black-brown pigment organelles will redistribute within the melanophore, and thereby cover or uncover the core, making complex color changes possible in the dermis. Previously, melanophores have only been cultured on flat surfaces. Here we mimic the three dimensional biological geometry in the frog dermis by culturing melanophores on fluorescent plastic microbeads. To demonstrate biosensing we use the hormones melatonin and α-melanocyte stimulating hormone (α-MSH) as lightening or darkening stimuli, respectively. Cellular responses were successfully demonstrated on single cell level by fluorescence microscopy, and in cell suspension by a fluorescence microplate reader and a previously demonstrated computer screen photo-assisted technique. The demonstrated principle is the first step towards "single well/multiple read-out" biosensor arrays based on suspensions of different selective-responding melanophores, each cultured on microbeads with distinctive spectral characteristics. By applying small amount of a clinical sample, or a candidate substance in early drug screening, to a single well containing combinations of melanophores on beads, multiple parameter read-outs will be possible. © 2004 Elsevier B.V. All rights reserved.

  • 9. Ashaduzzaman, M.
    et al.
    Anto Antony, A.
    Arul Murugan, Natarajan
    KTH, Skolan för bioteknologi (BIO), Teoretisk kemi och biologi.
    Deshpande, S. R.
    Turner, A. P. F.
    Tiwari, A.
    Studies on an on/off-switchable immunosensor for troponin T2015Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (δG<inf>coil</inf>=-6.0Kcal/mole) and 37°C (δG<inf>globular</inf>=-41.0kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with 'built-in' programmable interactions of antigen-antibody upon receiving stimuli.

  • 10.
    Ashaduzzaman, Md
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Dhaka, Bangladesh.
    Anto Antony, Aswathi
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Murugan, N. Arul
    Royal Institute Technology, Sweden.
    Deshpande, Swapneel R.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Tekidag AB, UCS, S-58330 Linkoping, Sweden.
    Studies on an on/off-switchable immunosensor for troponin T2015Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73, s. 100-107Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (Delta G(coil)=-6.0 Kcal/mole) and 37 degrees C (Delta G(globular)=-41.0 kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with built-in programmable interactions of antigen-antibody upon receiving stimuli.

  • 11.
    Asif, Muhammad H
    et al.
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Usman Ali, Syed M
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Nur, Omer
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Willander, Magnus
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Brännmark, Cecilia
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Strålfors, Peter
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Englund H, Ulrika
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Elinder, Fredrik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Danielsson, Bengt
    Pure and Applied Biochemistry, Lund University, Box 124, SE-221 00 Lund, Sweden.
    Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose2010Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 25, nr 10, s. 2205-2211Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration.

  • 12.
    Asif, Muhammad
    et al.
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Nour, Omer
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Willander, Magnus
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Danielsson, B.
    Department of Pure and Applied Biochemistry, Lund University, Box 124, SE-22100 Lund, Sweden.
    Selective calcium ion detection with functionalized ZnO nanorods-extendedgate MOSFET2009Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 24, nr 11, s. 3379-3382Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Zinc oxide nanorod-extended gate field effect transistor (MOSFET) is demonstrated for the detection of calcium (Ca2+) ions. ZnO nanorods were grown on the surface of a silver wire to produce an electrochemical nanosensor for selectively detecting Ca2+. The electrochemical response from the interaction between the ZnO nanorods and Ca2+ in an aqueous solution is coupled directly to the gate of a field effect transistor (MOSFET). The induced voltage change on the gate results in a measureable current response. In order to adapt the sensors for Ca2+ ions measurements in biological fluids with sufficient selectivity and stability, a plastic membrane coating containing ionophores was applied on the nanorods. The sensor exhibited a linear response within the range of interest from 1 μM to 1 mM. This work demonstrates a simple technique for sensitive detection of Ca2+ ions by efficient transfer of the chemical response directly to a standard electronic component producing a low impedance signal.

  • 13.
    Asif, Muhammad
    et al.
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Usman Ali, Syed
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Nour, Omer
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Willander, Magnus
    Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
    Englund, Ulrika
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Elinder, Fredrik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Functionalized ZnO nanorod-based selective magnesium ion sensor for intracellular measurements2010Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, nr 3, s. 1118-1123Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    ZnO nanorods were grown on a silver-coated tip of a borosilicate glass capillary (0.7 mu m in tip diameter) and used as selective potentiometric sensor of intracellular free Mg2+. To functionalize the ZnO nanorods for selectivity of Mg2+, a polymeric membrane with Mg2+-selective ionophores were coated on the surface of the ZnO nanorods. These functionalized ZnO nanorods exhibited a Mg2+-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode within the concentration range from 500 nM to 100 mM. Two types of cells, human adipocytes and frog oocytes, were used for the intracellular Mg2+ measurements. The intracellular concentration of free Mg2+ in human adipocytes and frog oocytes were 0.4-0.5 and 0.8-0.9 mM, respectively. Such type of nanoelectrode device paves the way to enable analytical measurements in single living cells and to sense other bio-chemical species at the intracellular level.

  • 14.
    Azzouzi, Sawsen
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Sousse, Tunisia.
    Mak, Wing Cheung
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Kor, Kamalodin
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Iranian National Institute Oceanog and Atmospher Science, Iran.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Ben Ali, Mounir
    University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten. ACREO SWEDISH ICE AB, Sweden.
    An integrated dual functional recognition/amplification bio-label for the one-step impedimetric detection of Micro-RNA-212017Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 92, s. 154-161Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Alteration in expression of miRNAs has been correlated with different cancer types, tumour stage and response to treatments. In this context, a structurally responsive oligonucleotide-based electrochemical impedimetric biosensor has been developed for the simple and sensitive detection of miRNA-21. A highly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold nanoparticles (AuNPs) to create an integrated, dual function bio-label (biotin-MB-AuNPs) for both biorecognition and signal generation. In the presence of target miRNA-21, hybridisation takes place resulting in the "activation" of the biotin-MB; this event makes the biotin group, which was previously "protected" by the steric hindrance of the MB stem-loop structure, accessible. The activated biotin-MB-AuNPs/miRNA complexes become available for capture, via supramolecular interaction, onto a nentravidin-modified electrode for electrochemical transduction. The binding event results in a decrease of the charge transfer resistance at the working electrode/electrolyte interface. The biosensor responded linearly in the range 1-1000 pM of miRNA-21, with a limit of detection of 0.3 pM, good reproducibility (Relative Standard deviation (RSD) =3.3%) and high selectivity over other miRNAs (i.e. miRNA221 and miRNA-205) sequences. Detection of miRNA-21 in spiked serum samples at clinically relevant levels (low pM range) was also demonstrated, thus illustrating the potential of the biosensor for point-of-care clinical applications. The proposed biosensor design, based on the combination of a neutravidin transducing surface and the dual-function biotin-MB-AuNPs bio-label, provides a simple and robust approach for detection of short-length nucleic acid targets, such as miRNAs.

  • 15. Bachinger, T.
    et al.
    Riese, U.
    Cell Culture Production, Pharmacia AB, S-112 87 Stockholm, Sweden.
    Eriksson, R.K.
    Cell Culture Production, Pharmacia AB, S-112 87 Stockholm, Sweden.
    Mandenius, Carl-Fredrik
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi.
    Gas sensor arrays for early detection of infection in mammalian cell culture2002Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 17, nr 5, s. 395-403Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The detection of bacterial infections in a mammalian cell culture process is realised using a gas sensor array. In production-scale and laboratory-scale cultivations of a perfused recombinant CHO-cell culture producing human blood coagulation Factor VIII, we show that the gas sensor array identifies bacterial contamination earlier than conventional methods. The sensitivity of the instrument is verified by inoculation of a blank cell culture medium with defined bacterial cell counts. © 2002 Elsevier Science B.V. All rights reserved.

  • 16.
    Bagheryan, Zahra
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. University of Mazandaran, Iran.
    Raoof, Jahan-Bakhsh
    University of Mazandaran, Iran.
    Golabi, Mohsen
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten. Acreo Swedish ICT AB, Sweden.
    Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 80, s. 566-573Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Fast and accurate detection of microorganisms is of key importance in clinical analysis and in food and water quality monitoring. Salmonella typhimurium is responsible for about a third of all cases of food borne diseases and consequently, its fast detection is of great importance for ensuring the safety of foodstuffs. We report the development of a label-free impedimetric aptamer-based biosensor for S. typhimurium detection. The aptamer biosensor was fabricated by grafting a diazonium-supporting layer onto screen printed carbon electrodes (SPEs), via electrochemical or chemical approaches, followed by chemical immobilisation of aminated-aptamer. FTIR-ATR, contact angle and electrochemical measurements were used to monitor the fabrication process. Results showed that electrochemical immobilisation of the diazonium-grafting layer allowed the formation of a denser aptamer layer, which resulted in higher sensitivity. The developed aptamer-biosensor responded linearly, on a logarithm scale, over the concentration range 1 x 10(1) to 1 x 10(8) CFU mL(-1), with a limit of quantification (LOQ) of 1 x 10(1) CFU mL(-1) and a limit of detection (LOD) of 6 CFU mL(-1). Selectivity studies showed that the aptamer biosensor could discriminate S. typhimurium from 6 other model bacteria strains. Finally, recovery studies demonstrated its suitability for the detection of S. typhimurium in spiked (1 x 10(2), 1 x 10(4) and 1 x 10(6) CFU mL(-1)) apple juice samples. (C) 2016 Elsevier B.V. All rights reserved.

  • 17.
    Basheer, Shabana
    et al.
    Central Food Technology Research Institute.
    Samyn, Dieter R.
    Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
    Hedström, Martin
    Lund university.
    Thakur, Munna Singh
    Central Food Technology Research Institute.
    Persson, Bengt L.
    Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
    Mattiasson, Bo
    Lund university.
    A membrane protein based biosensor: Use of a phosphate - H+ symporter membrane protein (Pho84) in the sensing of phosphate ions.2011Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 27, nr 1, s. 58-63Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A label free biosensor for direct detection of inorganic phosphate based on potential-step capacitance measurements has been developed. The high-affinity Pho84 plasma membrane phosphate/proton symporter of Saccharomyces cerevisiae was used as a sensing element. Heterologously expressed and purified Pho84 protein was immobilized on a self-assembled monolayer (SAM) on a capacitance electrode. Changes in capacitance were recorded upon exposure to phosphate compared to the control substance, phosphate analogue methylphosphonate. Hence, even without the explicit use of lipid membranes, the Pho84 membrane protein could retain its capacity of selective substrate binding, with a phosphate detection limit in the range of the apparent in vivo K(m). A linear increase in capacitance was monitored in the phosphate concentration range of 5-25 mu M. The analytical response of the capacitive biosensor is in agreement with that the transporter undergoes significant conformational changes upon exposure to inorganic phosphate, while exposure to the analogue only causes minor responses.

  • 18.
    Beni, Valerio
    et al.
    NMRC, University College, Cork, Ireland.
    Ghita, Mihaela
    PSiMedica, Malvern Hills Science Park, UK.
    Arrigan, Damien
    NMRC, University College, Cork, Ireland.
    Cyclic and pulse voltammetric study of dopamine at the interfacebetween two immiscible electrolyte solutions2005Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 20, nr 10, s. 2097-2103Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The detection of dopamine by differential pulse voltammetry (DPV) and square wave voltammetry (SWV) at the interface between twoimmiscible electrolyte solutions (ITIES) has been studied. Voltammetry at the liquid/liquid (water/1,2-dichloroethane) interface provides asimple method for overcoming the major problem associated with dopamine detection by voltammetry at solid electrodes: the co-existenceof ascorbate at higher concentrations. Selectivity for dopamine was achieved by the use of dibenzo-18-crown-6 as an ionophore for thefacilitated transfer voltammetry of protonated dopamine across the ITIES. Under these conditions, ascorbate is not transferred and hence doesnot interfere in the ion transfer current for dopamine. By use of DPV and SWV, the lowest concentration detectable can be lowered from ca.0.1mM (obtained with cyclic voltammetry) to 2 M. Evaluation of the effect of some other physiologically important species (acetylcholine,sodium, potassium and ammonium ions) on the dopamine transfer voltammetry has been studied, indicating the need for improved ionophoredesigns in order to achieve practically useful selectivity.

  • 19.
    Beni, Valerio
    et al.
    Universitat Rovira i Virgili, Tarragona, Spain.
    Hayes, Karen
    Universitat Rovira i Virgili, Tarragona, Spain.
    Mairal Lerga, Teresa
    Universitat Rovira i Virgili, Tarragona, Spain.
    O´Sullivan, Ciara K.
    Universitat Rovira i Virgili, Tarragona, Spain.
    Development of a gold nano-particle-based fluorescent molecular beacon fordetection of cystic fibrosis associated mutation2010Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, nr 2, s. 307-313Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Cystic fibrosis is one of the most common genetically inherited diseases in Northern Europe, consistingof an inherited defect of chloride transport in the epithelium. Of the several mutations related to CF, theF508 mutation occurs in ca. 70% of the cases. In this work the use of a gold nano-particle supportedfluorescence molecular beacon was investigated as an optical sensing platform for the detection of theF508 cystic fibrosis associated mutation. Different parameters such as molecular beacon design, Aunano-particle size, molecular beacon–nano-particle conjugation protocol, molecular beacon loading aswell as experimental conditions were evaluated. A 31-base long molecular beacon, containing a 15-baserecognition sequence specific for the mutant target, was linked via a thiol modified poly thymine linker(10 bases long) to a 13 nm gold nano-particle and was exposed to mutant and wild type targets, and aclear differentiation was achieved at target concentrations as low as 1 nM.

  • 20.
    Berti, Francesca
    et al.
    University Florence, Department Chemistry, I-50019 Florence, Italy.
    Todros, Silvia
    University Brescia, Department Chemistry and Phys, CNR INFM SENSOR Lab, I-25133 Brescia, Italy.
    Lakshmi, Dhana
    Cranfield University, Cranfield MK43 0AL, Beds, England.
    J. Whitcombe, Michael
    Cranfield University, Cranfield MK43 0AL, Beds, England.
    Chianella, Iva
    Cranfield University, Cranfield MK43 0AL, Beds, England.
    Ferroni, Matteo
    University Brescia, Department Chemistry and Phys, CNR INFM SENSOR Lab, I-25133 Brescia, Italy.
    A. Piletsky, Sergey
    Cranfield University, Cranfield MK43 0AL, Beds, England.
    P. F. Turner, Anthony
    Cranfield University, UK.
    Marrazza, Giovanna
    University Florence, Department Chemistry, I-50019 Florence, Italy.
    Quasi-monodimensional polyaniline nanostructures for enhanced molecularly imprinted polymer-based sensing2010Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, nr 2, s. 497-503Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Recent advances in nanotechnology have allowed significant progress in utilising cutting-edge techniques associated with nanomaterials and nano-fabrication to expand the scope and capability of biosensors to a new level of novelty and functionality. The aim of this work was the development and characterisation of conductive polyaniline (PANI) nanostructures for applications in electrochemical biosensing. We explore a simple, inexpensive and fast route to grow PANI nanotubes, arranged in an ordered structure directly on an electrode surface, by electrochemical polymerisation using alumina nanoporous membranes as a nano-mould. The deposited nanostructures have been characterised electrochemically and morphologically prior to grafting with a molecularly imprinted polymer (MIP) receptor in order to create a model sensor for catechol detection. In this way, PANI nanostructures resulted in a conductive nanowire system which allowed direct electrical connection between the electrode and the synthetic receptor (MIP). To our knowledge, this is the first example of integration between molecularly imprinted polymers and PANI nanostructured electrodes. The advantages of using nanostructures in this particular biosensing application have been evaluated by comparing the analytical performance of the sensor with an analogous non-nanostructured MIP-sensor for catechol detection that was previously developed. A significantly lower limit of detection for catechol has been obtained (29 nM, one order of magnitude), thus demonstrating that the nanostructures are capable of improving the analytical performance of the sensor. (C) 2010 Elsevier B.V. All rights reserved.

  • 21.
    Bini, Alessandra
    et al.
    University of Florence.
    Mascini, Marcello
    University of Teramo.
    Mascini, Marco
    University of Florence.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och Bioelektronik. Linköpings universitet, Tekniska högskolan.
    Selection of thrombin-binding aptamers by using computational approach for aptasensor application2011Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, nr 11, s. 4411-4416Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The possibility of introducing a computationally assisted method to study aptamer-protein interaction was evaluated with the aim of streamlining the screening and selection of new aptamers. Starting from information on the 15-mer (5-GGTTGGTGTGGTTGG-3 thrombin binding aptamer (TBA), a library of mutated DNA sequences (994 elements) was generated and screened using shapegauss a shape-based scoring function from openeye software to generate computationally derived binding scores. The TBA and three other mutated oligonucleotides, selected on the basis of their binding score (best, medium, worst), were incorporated into surface plasmon resonance (SPR) biosensors. By reducing the ionic strength (binding buffer, 50 mMTrisHC1pH 7.4, 140 mM NaCl, 1 mM MgCl(2), diluted 1:50) in order to match the simulated condition, the analytical performances of the four oligonucleotide sequences were compared using signal amplitude, sensitivity (slope), linearity (R(2)) and reproducibility (CVav %). The experimental results were in agreement with the simulation findings.

  • 22.
    Bonini, Francesca
    et al.
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
    Piletsky, Sergey
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
    P. F. Turner, Anthony
    Cranfield University, UK.
    Speghini, Adolfo
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
    Bossi, Alessandra
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Cranfield Hlth, Bedford MK45 4DT, England; .
    Surface imprinted beads for the recognition of human serum albumin2007Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 22, nr 10-sep, s. 2322-2328Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology. (c) 2007 Elsevier B.V. All rights reserved.

  • 23.
    Borsa, Baris A
    et al.
    Istanbul Kemerburgaz University, School of Medicine, Istanbul 34217, Turkey.
    Tuna, Bilge G
    Yeditepe University, School of Medicine, Department of Biophysics, Istanbul Turkey.
    Hernandez, Frank J
    SOMAprobes S.L., Science and Technology Park of Gipuzkoa, San Sebastian 20009, Spain.
    Hernandez, Luiza I
    SOMAprobes S.L., Science and Technology Park of Gipuzkoa, San Sebastian 20009, Spain.
    Bayramoglu, Gulay
    Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Ankara, Turkey; Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Ankara, Turkey.
    Arica, M Yakup
    Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Ankara, Turkey.
    Ozalp, V Cengiz
    Istanbul Kemerburgaz University, School of Medicine, Istanbul 34217, Turkey.
    Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates.2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 86, s. 27-32Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, an effective system, Nanokeepers as previously reported, were used for triggered release of confined fluorophores, and hence specific detection of S. aureus on nuclease activity was obtained. The interference from blood components for fluorescent quantification was eliminated by a pre-purification by aptamer-functionalized silica magnetic nanoparticles. The reported assay system was exclusively formed by nucleic acid oligos and magnetic or mesoporous silica nanoparticles, that can be used on blood samples in a stepwise manner. The assay was successfully used as a sensing platform for the specific detection of S. aureus cells as low as 682 CFU in whole blood.

  • 24.
    Bossi, A.
    et al.
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Silsoe MK45 4DT, Beds, England; .
    Bonini, F.
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Silsoe MK45 4DT, Beds, England; .
    P. F. Turner, A.
    Cranfield University, UK.
    A. Piletsky, S.
    University Verona, Department Science and Technology, I-37134 Verona, Italy; Cranfield University, Silsoe MK45 4DT, Beds, England; .
    Molecularly imprinted polymers for the recognition of proteins: The state of the art2007Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 22, nr 6, s. 1131-1137Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Molecular imprinting has proved to be an effective technique for the creation of recognition sites on a polymer scaffold. Protein imprinting has been a focus for many chemists working in the area of molecular recognition, since the creation of synthetic polymers that can specifically recognise proteins is a very challenging but potentially extremely rewarding objective. It is expected that molecularly imprinted polymers (MIPs) with specificity for proteins will find application in medicine, diagnostics, proteomics, environmental analysis, sensors and drug delivery. In this review, the authors provide an overview of the progress achieved in the decade between 1994 and 2005, with respect to the challenging area of MIPs for protein recognition. The discussion furnishes a comparative analysis of different approaches developed, underlining their relative advantages and disadvantages and highlighting trends and possible future directions. (c) 2006 Elsevier B.V. All rights reserved.

  • 25.
    Bossi, Alessandra
    et al.
    Dipartimento Scientifico e Tecnologico, Università di Verona, Verona, Italy.
    Rivetti, Claudio
    Dipartimento di Biochimica e Biologia Molecolare, Università di Parma, Parma, Italy.
    Mangiarotti, Laura
    Dipartimento di Biochimica e Biologia Molecolare, Università di Parma, Parma, Italy.
    Whitcombe, Michael J.
    Cranfield Health, Cranfield University at Silsoe, Bedfordshire, UK.
    Turner, Anthony P F
    Cranfield University, UK.
    Piletsky, Sergey A.
    Cranfield Health, Cranfield University at Silsoe, Bedfordshire, UK.
    Patterned gallium surfaces as molecular mirrors2007Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 23, nr 2, s. 290-294Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    An entirely new means of printing molecular information on a planar film, involving casting nanoscale impressions of the template protein molecules in molten gallium, is presented here for the first time. The metallic imprints not only replicate the shape and size of the proteins used as template. They also show specific binding for the template species. Such a simple approach to the creation of antibody-like properties in metallic mirrors can lead to applications in separations, microfluidic devices, and the development of new optical and electronic sensors, and will be of interest to chemists, materials scientists, analytical specialists, and electronic engineers.

  • 26. Carinelli, S.
    et al.
    Kühnemund, M.
    Nilsson, Mats
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik. Stockholms universitet, Science for Life Laboratory (SciLifeLab).
    Pividori, M. I.
    Yoctomole electrochemical genosensing of Ebola virus cDNA by rolling circle and circle to circle amplification2017Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 93, s. 65-71Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This work addresses the design of an Ebola diagnostic test involving a simple, rapid, specific and highly sensitive procedure based on isothermal amplification on magnetic particles with electrochemical readout. Ebola padlock probes were designed to detect a specific L-gene sequence present in the five most common Ebola species. Ebola cDNA was amplified by rolling circle amplification (RCA) on magnetic particles. Further re-amplification was performed by circle-to-circle amplification (C2CA) and the products were detected in a double-tagging approach using a biotinylated capture probe for immobilization on magnetic particles and a readout probe for electrochemical detection by square-wave voltammetry on commercial screen-printed electrodes. The electrochemical genosensor was able to detect as low as 200 ymol, corresponding to 120 cDNA molecules of L-gene Ebola virus with a limit of detection of 33 cDNA molecules. The isothermal double-amplification procedure by C2CA combined with the electrochemical readout and the magnetic actuation enables the high sensitivity, resulting in a rapid, inexpensive, robust and user-friendly sensing strategy that offers a promising approach for the primary care in low resource settings, especially in less developed countries.

  • 27.
    Carinelli, S.
    et al.
    Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra, Spain..
    Kühnemund, Malte
    Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi.
    Nilsson, Mats
    Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi. Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Box 1031, SE-17I21 Solna, Sweden..
    Pividori, M. I.
    Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra, Spain..
    Yoctomole electrochemical genosensing of Ebola virus cDNA by rolling circle and circle to circle amplification2017Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 93, s. 65-71Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This work addresses the design of an Ebola diagnostic test involving a simple, rapid, specific and highly sensitive procedure based on isothermal amplification on magnetic particles with electrochemical readout. Ebola padlock probes were designed to detect a specific L-gene sequence present in the five most common Ebola species. Ebola cDNA was amplified by rolling circle amplification (RCA) on magnetic particles. Further re-amplification was performed by circle-to-circle amplification (C2CA) and the products were detected in a double-tagging approach using a biotinylated capture probe for immobilization on magnetic particles and a readout probe for electrochemical detection by square-wave voltammetry on commercial screen-printed electrodes. The electrochemical genosensor was able to detect as low as 200 ymol, corresponding to 120 cDNA molecules of L-gene Ebola virus with a limit of detection of 33 cDNA molecules. The isothermal double-amplification procedure by C2CA combined with the electrochemical readout and the magnetic actuation enables the high sensitivity, resulting in a rapid, inexpensive, robust and user-friendly sensing strategy that offers a promising approach for the primary care in low resource settings, especially in less developed countries.

  • 28.
    Chen, B
    et al.
    Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
    Bestetti, G
    Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
    Day, RM
    Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England; .
    Turner, APF
    Cranfield University, UK.
    The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin1998Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 13, nr 08-jul, s. 779-785Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA(1c)), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262 144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA(1c). The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin. (C) 1998 Elsevier Science S.A. All rights reserved.

  • 29.
    Chianella, I
    et al.
    Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
    Piletsky, SA
    Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
    Tothill, IE
    Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
    Chen, B
    Cranfield University, Institute Biosci and Technology, Silsoe MK45 4DT, Beds, England; .
    Turner, APF
    Cranfield University, UK.
    MIP-based solid phase extraction cartridges combined with MIP-based sensors for the detection of microcystin-LR2003Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 18, nr 03-feb, s. 119-127Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Microsystin-LR is one of the most widespread and dangerous toxins produced by the freshwater Cyanobacteria. The contamination of water supplies with microcystin-LR has been reported in several areas around the world and the development of an easy-to-use, rapid, robust and inexpensive sensor for this toxin is urgently required. In this work an artificial receptor for microcystin-LR was synthesised using the technique of molecular imprinting. The composition of the molecularly imprinted polymer (MIP) was optimised using computer modelling. The synthesised polymer was used both as a material for solid-phase extraction (SPE) and as a sensing element in a piezoelectric sensor. Using the combination of SPE followed by detection with a piezoelectric sensor the minimum detectable amount of toxin was 0.35 nM. The use of MIP-SPE provided up to 1000 fold preconcentration, which was more than sufficient for achieving the required detection limit for microcystin-LR in drinking water (I nM). This work is the first example where the same MIP receptor has been used successfully for both SPE and the corresponding sensor. (C) 2002 Elsevier Science B.V. All rights reserved.

  • 30.
    Comina, German
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska fakulteten. Not Found:Linkoping Univ, Opt Devices Lab, Dept Phys Chem and Biol IFM, S-58183 Linkoping, Sweden.
    Suska, Anke
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska fakulteten.
    Filippini, Daniel
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska fakulteten.
    Towards autonomous lab-on-a-chip devices for cell phone biosensing2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 77, s. 1153-1167Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Modern cell phones are a ubiquitous resource with a residual capacity to accommodate chemical sensing and biosensing capabilities. From the different approaches explored to capitalize on such resource, the use of autonomous disposable lab-on-a-chip (LOC) devices conceived as only accessories to complement cell phones underscores the possibility to entirely retain cell phones ubiquity for distributed biosensing. The technology and principles exploited for autonomous LOC devices are here selected and reviewed focusing on their potential to serve cell phone readout configurations. Together with this requirement, the central aspects of cell phones resources that determine their potential for analytical detection are examined. The conversion of these LOC concepts into universal architectures that are readable on unaccessorized phones is discussed within this context. (C) 2015 Elsevier B.V. All rights reserved.

  • 31.
    Cánovas, Rocío
    et al.
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi.
    Cuartero, Maria
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi.
    Crespo, Gaston A.
    KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi.
    Modern creatinine (Bio)sensing: Challenges of point-of-care platforms2019Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 130, s. 110-124Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    The importance of knowing creatinine levels in the human body is related to the possible association with renal, muscular and thyroid dysfunction. Thus, the accurate detection of creatinine may indirectly provide information surrounding those functional processes, therefore contributing to the management of the health status of the individual and early diagnosis of acute diseases. The questions at this point are: to what extent is creatinine information clinically relevant?; and do modern creatinine (bio)sensing strategies fulfil the real needs of healthcare applications? The present review addresses these questions by means of a deep analysis of the creatinine sensors reported in the literature over the last five years. There is a wide range of techniques for detecting creatinine, most of them based on optical readouts (20 of the 33 papers collected in this review). However, the use of electrochemical techniques (13 of the 33 papers) is recently emerging in alignment with the search for a definitive and trustworthy creatinine detection at the point-of-care level. In this sense, biosensors (7 of the 33 papers) are being established as the most promising alternative over the years. While creatinine levels in the blood seem to provide better information about patient status, none of the reported sensors display adequate selectivity in such a complex matrix. In contrast, the analysis of other types of biological samples (e.g., saliva and urine) seems to be more viable in terms of simplicity, cross-selectivity and (bio)fouling, besides the fact that its extraction does not disturb individual's well-being. Consequently, simple tests may likely be used for the initial check of the individual in routine analysis, and then, more accurate blood detection of creatinine could be necessary to provide a more genuine diagnosis and/or support the corresponding decision-making by the physician. Herein, we provide a critical discussion of the advantages of current methods of (bio)sensing of creatinine, as well as an overview of the drawbacks that impede their definitive point-of-care establishment.

  • 32.
    Dev, Apurba
    et al.
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Material- och nanofysik. Uppsala University, Sweden.
    Horak, J.
    Kaiser, A.
    Yuan, X.
    Perols, A.
    Björk, P.
    Karlström, A. E.
    Kleimann, P.
    Linnros, Jan
    KTH, Skolan för informations- och kommunikationsteknik (ICT), Material- och nanofysik, Materialfysik, MF.
    Electrokinetic effect for molecular recognition: A label-free approach for real-time biosensing2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 82, s. 55-63Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present a simple and inexpensive method for label-free detection of biomolecules. The method monitors the changes in streaming current in a fused silica capillary as target biomolecules bind to immobilized receptors on the inner surface of the capillary. To validate the concept, we show detection and time response of different protein-ligand and protein-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z domain-immunoglobulin G (IgG). We show that specific binding of these biomolecules can be reliably monitored using a very simple setup. Using sequential injections of various proteins at a diverse concentration range and as well as diluted human serum we further investigate the capacity of the proposed technique to perform specific target detection from a complex sample. We also investigate the time for the signal to reach equilibrium and its dependence on analyte concentration and demonstrate that the current setup can be used to detect biomolecules at a concentration as low as 100 pM without requiring any advanced device fabrication procedures. Finally, an analytical model based on diffusion theory has been presented to explain the dependence of the saturation time on the analyte concentration and capillary dimensions and how reducing length and inner diameter of the capillary is predicted to give faster detection and in practice also lower limit of detection. © 2016 Elsevier B.V.

  • 33.
    Dev, Apurba
    et al.
    KTH Royal Institute of Technology, Sweden; Uppsala University, Sweden.
    Horak, Josef
    KTH Royal Institute of Technology, Sweden.
    Kaiser, Andreas
    KTH Royal Institute of Technology, Sweden.
    Yuan, Xichen
    UCBL Claude Bernard University Lyon 1, France.
    Perols, Anna
    KTH Royal Institute of Technology, Sweden.
    Björk, Per
    RISE., Swedish ICT, Acreo.
    Eriksson Karlström, Amelie
    KTH Royal Institute of Technology, Sweden.
    Kleimann, Pascal
    UCBL Claude Bernard University Lyon 1, France.
    Linnros, Jan
    KTH Royal Institute of Technology, Sweden.
    Electrokinetic effect for molecular recognition: A label-free approach for real-time biosensing2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 82, s. 55-63Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present a simple and inexpensive method for label-free detection of biomolecules. The method monitors the changes in streaming current in a fused silica capillary as target biomolecules bind to immobilized receptors on the inner surface of the capillary. To validate the concept, we show detection and time response of different protein-ligand and protein-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z domain-immunoglobulin G (IgG). We show that specific binding of these biomolecules can be reliably monitored using a very simple setup. Using sequential injections of various proteins at a diverse concentration range and as well as diluted human serum we further investigate the capacity of the proposed technique to perform specific target detection from a complex sample. We also investigate the time for the signal to reach equilibrium and its dependence on analyte concentration and demonstrate that the current setup can be used to detect biomolecules at a concentration as low as 100 pM without requiring any advanced device fabrication procedures. Finally, an analytical model based on diffusion theory has been presented to explain the dependence of the saturation time on the analyte concentration and capillary dimensions and how reducing length and inner diameter of the capillary is predicted to give faster detection and in practice also lower limit of detection.

  • 34.
    Dev, Apurba
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets elektronik. KTH Royal Inst Technol, Sch Informat & Commun Technol, Dept Mat & Nano Phys, S-16440 Kista, Sweden.
    Horak, Josef
    KTH Royal Inst Technol, AlbaNova Univ Ctr, Sch Biotechnol, Div Prot Technol, S-10691 Stockholm, Sweden..
    Kaiser, Andreas
    KTH Royal Inst Technol, AlbaNova Univ Ctr, Sch Biotechnol, Div Prot Technol, S-10691 Stockholm, Sweden..
    Yuan, Xichen
    Univ Lyon 1, INL, UMR 5270, CNRS,UCBL,INSA,ECL, Bat Leon Brillouin, F-69622 Villeurbanne, France..
    Perols, Anna
    KTH Royal Inst Technol, AlbaNova Univ Ctr, Sch Biotechnol, Div Prot Technol, S-10691 Stockholm, Sweden..
    Björk, Per
    Swedish ICT Acre AB, SE-16440 Stockholm, Sweden..
    Karlström, Amelie Eriksson
    KTH Royal Inst Technol, AlbaNova Univ Ctr, Sch Biotechnol, Div Prot Technol, S-10691 Stockholm, Sweden..
    Kleimann, Pascal
    Univ Lyon 1, INL, UMR 5270, CNRS,UCBL,INSA,ECL, Bat Leon Brillouin, F-69622 Villeurbanne, France..
    Linnros, Jan
    KTH Royal Inst Technol, Sch Informat & Commun Technol, Dept Mat & Nano Phys, S-16440 Kista, Sweden..
    Electrokinetic effect for molecular recognition: A label-free approach for real-time biosensing2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 82, s. 55-63Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present a simple and inexpensive method for label-free detection of biomolecules. The method monitors the changes in streaming current in a fused silica capillary as target biomolecules bind to immobilized receptors on the inner surface of the capillary. To validate the concept, we show detection and time response of different protein-ligand and protein-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z domain-immunoglobulin G (IgG). We show that specific binding of these biomolecules can be reliably monitored using a very simple setup. Using sequential injections of various proteins at a diverse concentration range and as well as diluted human serum we further investigate the capacity of the proposed technique to perform specific target detection from a complex sample. We also investigate the time for the signal to reach equilibrium and its dependence on analyte concentration and demonstrate that the current setup can be used to detect biomolecules at a concentration as low as 100 pM without requiring any advanced device fabrication procedures. Finally, an analytical model based on diffusion theory has been presented to explain the dependence of the saturation time on the analyte concentration and capillary dimensions and how reducing length and inner diameter of the capillary is predicted to give faster detection and in practice also lower limit of detection.

  • 35. Donolato, Marco
    et al.
    Antunes, Paula
    Zardán Gómez de la Torre, Teresa
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Nanoteknologi och funktionella material.
    Hwu, En-Te
    Chen, Ching-Hsiu
    Burger, Robert
    Rizzi, Giovanni
    Bosco, Filippo Giacomo
    Strömme, Maria
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Nanoteknologi och funktionella material.
    Boisen, Anja
    Hansen, Mikkel Fougt
    Quantification of rolling circle amplified DNA using magnetic nanobeads and a Blu-ray optical pick-up unit2015Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 67, nr SI, s. 649-655Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present the first implementation of a Blu-ray optical pickup unit (OPU) for the high-performance low-cost readout of a homogeneous assay in a multichamber microfluidic disc with a chamber thickness of 600μm. The assay relies on optical measurements of the dynamics of magnetic nanobeads in an oscillating magnetic field applied along the light propagation direction. The laser light provided by the OPU is transmitted through the sample chamber and reflected back onto the photo detector array of the OPU via a mirror. Spectra of the 2nd harmonic photo detector signal vs. the frequency of the applied magnetic field show a characteristic peak due to freely rotating magnetic nanobeads. Beads bound to ~1μm coils of DNA formed off-chip by padlock probe recognition and rolling circle amplification show a different dynamics and the intensity of the characteristic peak decreases. We have determined the optimum magnetic bead concentration to 0.1mg/mL and have measured the response vs. concentration of DNA coils formed from Escherichia Coli. We have found a limit of detection of 10pM and a dynamic range of about two orders of magnitude, which is comparable to the performance obtained using costly and bulky laboratory equipment. The presented device leverages on the advanced but low-cost technology of Blu-ray OPUs to provide a low-cost and high-performance magnetic bead-based readout of homogeneous bioassays. The device is highly flexible and we have demonstrated its use on microfluidic chambers in a disc with a thickness compatible with current optical media mass-production facilities.

  • 36.
    Filippini, Daniel
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Andersson, Tony
    Linköpings universitet, Institutionen för medicin och vård, Farmakologi. Linköpings universitet, Hälsouniversitetet.
    Svensson, Samuel
    Linköpings universitet, Institutionen för medicin och vård, Farmakologi. Linköpings universitet, Hälsouniversitetet.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Hälsouniversitetet.
    Microplate based biosensing with a computer screen aided technique2003Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 19, nr 1, s. 35-41Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Melanophores, dark pigment cells from the frog Xenopus laevis, have the ability to change light absorbance upon stimulation by different biological agents. Hormone exposure (e.g. melatonin or α-melanocyte stimulating hormone) has been used here as a reversible stimulus to test a new compact microplate reading platform. As an application, the detection of the asthma drug formoterol in blood plasma samples is demonstrated. The present system utilizes a computer screen as a (programmable) large area light source, and a standard web camera as recording media enabling even kinetic microplate reading with a versatile and broadly available platform, which suffices to evaluate numerous bioassays. Especially in the context of point of care testing or self testing applications these possibilities become advantageous compared with highly dedicated comparatively expensive commercial systems.

  • 37.
    Filippini, Daniel
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
    Tejle, Katarina
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för molekylär och klinisk medicin, Medicinsk mikrobiologi.
    Lundström, Ingemar
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
    ELISA test for anti-neutrophil cytoplasm antibodies detection evaluated by a computer screen photo-assisted technique2005Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 21, nr 2, s. 266-272Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The computer screen photo-assisted technique (CSPT), a method for substance classification based on spectral fingerprinting, which involves just a computer screen and a web camera as measuring platform is used here for the evaluation of a prospective enzyme-linked immunosorbent assay (ELISA). A anti-neutrophil cytoplasm antibodies (ANCA-ELISA) test, typically used for diagnosing patients suffering from chronic inflammatory disorders in the skin, joints, blood vessels and other tissues is comparatively tested with a standard microplate reader and CSPT, yielding equivalent results at a fraction of the instrumental costs. The CSPT approach is discussed as a distributed measuring platform allowing decentralized measurements in routine applications, whereas keeping centralized information management due to its natural network embedded operation. © 2004 Elsevier B.V. All rights reserved.

  • 38.
    Fischer, U.
    et al.
    Institüt für Diabetes “Gerhard Katsch” der Ernst—Moritz—Arndt Universität Greifswald, Karlsburg, Germany.
    Alcock, S.
    Cranfield Biotechnology Centre, Cranfield University, UK.
    Turner, Anthony P. F.
    Cranfield University, UK.
    Assessment of devices for in vivo monitoring of chemical species1995Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 10, nr 5, s. xxiii-xxxArtikkel i tidsskrift (Annet vitenskapelig)
  • 39.
    Fock, Jeppe
    et al.
    Technical University of Denmark.
    Parmvi, Mattias
    Technical University of Denmark;Blusense Diagnost, Box 68,Fruebjergvej 3, DK-2100 Copenhagen O, Denmark.
    Strömberg, Mattias
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Svedlindh, Peter
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Donolato, Marco
    Technical University of Denmark; Blusense Diagnost, Box 68,Fruebjergvej 3, DK-2100 Copenhagen O, Denmark.
    Fougt Hansen, Mikkel
    Technical University of Denmark.
    Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein2017Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 88, s. 94-100Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    There is an increasing need to develop biosensor methods that are highly sensitive and, that can be combined with low-cost consumables. The use of magnetic nanoparticles (MNPs) is attractive because their detection is compatible with low-cost disposables and because application of a magnetic field can be used to accelerate assay kinetics. We present the first study and comparison of the performance of magnetic susceptibility measurements and a newly proposed optomagnetic method. For the comparison we use the C-reactive protein (CRP) induced agglutination of identical samples of 100 nm MNPs conjugated with CRP antibodies. Both methods detect agglutination as a shift to lower frequencies in measurements of the dynamics in response to an applied oscillating magnetic field. The magnetic susceptibility method probes the magnetic response whereas the optomagnetic technique probes the modulation of laser light transmitted through the sample. The two techniques provided highly correlated results upon agglutination when they measure the decrease of the signal from the individual MNPs (turn-off detection strategy), whereas the techniques provided different results, strongly depending on the read-out frequency, when detecting the signal due to MNP agglomerates (turn-on detection strategy). These observations are considered to be caused by differences in the volume-dependence of the magnetic and optical signals from agglomerates. The highest signal from agglomerates was found in the optomagnetic signal at low frequencies.

  • 40.
    Fällman, Erik
    et al.
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
    Schedin, Staffan
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för tillämpad fysik och elektronik.
    Jass, Jana
    Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
    Andersson, Magnus
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
    Uhlin, Bernt Eric
    Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
    Axner, Ove
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
    Optical tweezers based force measurement system for quantitating binding interactions: system design and application for the study of bacterial adhesion2004Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 19, nr 11, s. 1429-1437Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    An optical force measurement system for quantitating forces in the pN range between micrometer-sized objects has been developed. The system was based upon optical tweezers in combination with a sensitive position detection system and constructed around an inverted microscope. A trapped particle in the focus of the high numerical aperture microscope-objective behaves like an omnidirectional mechanical spring in response to an external force. The particle’s displacement from the equilibrium position is therefore a direct measure of the exerted force. A weak probe laser beam, focused directly below the trapping focus, was used for position detection of the trapped particle (a polystyrene bead). The bead and the condenser focus the light to a distinct spot in the far field, monitored by a position sensitive detector. Various calibration procedures were implemented in order to provide absolute force measurements. The system has been used to measure the binding forces between Escherichia coli bacterial adhesins and galabiose-functionalized beads

  • 41.
    Gabig-Ciminska, Magdalena
    et al.
    KTH, Tidigare Institutioner, Bioteknologi.
    Holmgren, Anders
    KTH, Tidigare Institutioner, Bioteknologi.
    Andresen, Heiko
    KTH, Tidigare Institutioner, Bioteknologi.
    Barken, K. B.
    Wumpelmann, M.
    Albers, J.
    Hintsche, R.
    Breitenstein, A.
    Neubauer, P.
    Los, M.
    Czyz, A.
    Wegrzyn, G.
    Silfversparre, G.
    Jurgen, B.
    Schweder, T.
    Enfors, Sven-Olof
    KTH, Tidigare Institutioner, Bioteknologi.
    Electric chips for rapid detection and quantification of nucleic acids2004Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 19, nr 6, s. 537-546Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A silicon chip-based electric detector coupled to bead-based sandwich hybridization (BBSH) is presented as an approach to perform rapid analysis of specific nucleic acids. A microfluidic platform incorporating paramagnetic beads with immobilized capture probes is used for the biorecognition steps. The protocol involves simultaneous sandwich hybridization of a single-stranded nucleic acid target with the capture probe on the beads and with a detection probe in the reaction solution, followed by enzyme labeling of the detection probe, enzymatic reaction, and finally, potentiometric measurement of the enzyme product at the chip surface. Anti-DIG-alkaline phosphatase conjugate was used for the enzyme labeling of the DIG-labeled detection probe. p-Aminophenol phosphate (pAPP) was used as a substrate. The enzyme reaction product, p-aminophenol (pAP), is oxidized at the anode of the chip to quinoneimine that is reduced back to pAP at the cathode. The cycling oxidation and reduction of these compounds result in a current producing a characteristic signal that can be related to the concentration of the analyte. The performance of the different steps in the assay was characterized using in vitro synthesized RNA oligonucleotides and then the instrument was used for analysis of 16S rRNA in Escherichia coli extract. The assay time depends on the sensitivity required. Artificial RNA target and 16S rRNA, in amounts ranging from 10(11) to 10(10) molecules, were assayed within 25 min and 4 h, respectively.

  • 42.
    Golabi, Mohsen
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Kuralay, Filiz
    Department of Chemistry, Faculty of Arts and Sciences, Ordu University, Ordu, Turkey.
    Jager, Edwin
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Beni, Valerio
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Acreo Swedish ACT AB, Norrköping, Sweden.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Electrochemical bacterial detection using poly(3-aminophenylboronic acid)-based imprinted polymer.2017Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 93, s. 87-93Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Biosensors can deliver the rapid bacterial detection that is needed in many fields including food safety, clinical diagnostics, biosafety and biosecurity. Whole-cell imprinted polymers have the potential to be applied as recognition elements in biosensors for selective bacterial detection. In this paper, we report on the use of 3-aminophenylboronic acid (3-APBA) for the electrochemical fabrication of a cell-imprinted polymer (CIP). The use of a monomer bearing a boronic acid group, with its ability to specifically interact with cis-diol, allowed the formation of a polymeric network presenting both morphological and chemical recognition abilities. A particularly beneficial feature of the proposed approach is the reversibility of the cis-diol-boronic group complex, which facilitates easy release of the captured bacterial cells and subsequent regeneration of the CIP. Staphylococcus epidermidis was used as the model target bacteria for the CIP and electrochemical impedance spectroscopy (EIS) was explored for the label-free detection of the target bacteria. The modified electrodes showed a linear response over the range of 103–107 cfu/mL. A selectivity study also showed that the CIP could discriminate its target from non-target bacteria having similar shape. The CIPs had high affinity and specificity for bacterial detection and provided a switchable interface for easy removal of bacterial cell.

  • 43.
    Golabi, Mohsen
    et al.
    Linköping University, Sweden.
    Kuralay, Filiz
    Ordu University, Turkey.
    Jager, Edwin W. H.
    Linköping University, Sweden.
    Beni, Valerio
    RISE - Research Institutes of Sweden, ICT, Acreo. Linköping University, Sweden.
    Turner, Anthony P. F.
    Linköping University, Sweden.
    Electrochemical bacterial detection using poly(3-aminophenylboronic acid)-based imprinted polymer2017Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 93, s. 87-93Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Biosensors can deliver the rapid bacterial detection that is needed in many fields including food safety, clinical diagnostics, biosafety and biosecurity. Whole-cell imprinted polymers have the potential to be applied as recognition elements in biosensors for selective bacterial detection. In this paper, we report on the use of 3-aminophenylboronic acid (3-APBA) for the electrochemical fabrication of a cell-imprinted polymer (CIP). The use of a monomer bearing a boronic acid group, with its ability to specifically interact with cis-diol, allowed the formation of a polymeric network presenting both morphological and chemical recognition abilities. A particularly beneficial feature of the proposed approach is the reversibility of the cis-diol-boronic group complex, which facilitates easy release of the captured bacterial cells and subsequent regeneration of the CIP. Staphylococcus epidermidis was used as the model target bacteria for the CIP and electrochemical impedance spectroscopy (EIS) was explored for the label-free detection of the target bacteria. The modified electrodes showed a linear response over the range of 103–107 cfu/mL. A selectivity study also showed that the CIP could discriminate its target from non-target bacteria having similar shape. The CIPs had high affinity and specificity for bacterial detection and provided a switchable interface for easy removal of bacterial cell.

  • 44.
    Han, Yuanyuan
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Tillämpad materialvetenskap.
    Qiu, Zhen
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
    Nawale, Ganesh N.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Varghese, Oommen P.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Hilborn, Jöns
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - Ångström, Polymerkemi.
    Tian, Bo
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik. Tech Univ Denmark, Dept Micro & Nanotechnol, DK-2800 Kongens Lyngby, Denmark.
    Leifer, Klaus
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Tillämpad materialvetenskap.
    MicroRNA detection based on duplex-specific nuclease-assisted target recycling and gold nanoparticle/graphene oxide nanocomposite-mediated electrocatalytic amplification2019Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 127, s. 188-193Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    DNA technology based bio-responsive nanomaterials have been widely studied as promising tools for biomedical applications. Gold nanoparticles (AuNPs) and graphene oxide (GO) sheets are representative zero- and two-dimensional nanomaterials that have long been combined with DNA technology for point-of-care diagnostics. Herein, a cascade amplification system based on duplex-specific nuclease (DSN)-assisted target recycling and electrocatalytic water-splitting is demonstrated for the detection of microRNA. Target microRNAs can form DNA: RNA heteroduplexes with DNA probes on the surface of AuNPs, which can be hydrolyzed by DSN. MicroRNAs are preserved during the reaction and released into the suspension for the digestion of multiple DNA probes. After the DSN-based reaction, AuNPs are collected and mixed with GO to form AuNP/GO nanocomposite on an electrode for the following electrocatalytic amplification. The utilization of AuNP/GO nanocomposite offers large surface area, exceptional affinity to water molecules, and facilitated mass diffusion for the water-splitting reaction. For let-7b detection, the proposed biosensor achieved a limit detection of 1.5 fM in 80 min with a linear detection range of approximately four orders of magnitude. Moreover, it has the capability of discriminating non-target microRNAs containing even single-nucleotide mismatches, thus holding considerable potential for clinical diagnostics.

  • 45.
    Hansson, Kenny
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Johansen, Knut
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Wetterö, Jonas
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för molekylär och klinisk medicin, Reumatologi.
    Klenkar, Goran
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik.
    Benesch, Johan
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Lundström, Ingemar
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
    Lindahl, Tomas
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för biomedicin och kirurgi, Avdelningen för klinisk kemi. Östergötlands Läns Landsting, Laboratoriemedicinskt centrum, Klinisk kemi.
    Tengvall, Pentti
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
    Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions2007Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 23, nr 2, s. 261-268Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A surface plasmon resonance (SPR) based flow chamber device was designed for real time detection of blood coagulation and platelet adhesion in platelet rich plasma (PRP) and whole blood. The system allowed the detection of surface interactions throughout the 6 mm length of the flow chamber. After deposition of thromboplastin onto a section of the sensor surface near the inlet of the flow chamber, coagulation was detected downstream of this position corresponding to a SPR signal of 7 to 8 mRIU (7 to 8 ng/mm2). A nonmodified control surface induced coagulation 3.5 times slower. Platelet adhesion to gold and fibrinogen coated surfaces in the magnitude of 1.25 and 1.66 mRIU was also shown with platelets in buffer, respectively. SPR responses obtained with PRP and whole blood on surfaces that were methylated or coated with von Willebrand factor (vWF), fibrinogen, or collagen, coincided well with platelet adhesion as observed with fluorescence microscopy in parallel experiments. The present SPR detection equipped flow chamber system is a promising tool for studies on coagulation events and blood cell adhesion under physiological flow conditions, and allows monitoring of short-range surface processes in whole blood. © 2007 Elsevier B.V. All rights reserved.

  • 46.
    Hansson, Kenny
    et al.
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Tengvall, Pentti
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Rånby, Mats
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Lindahl, Tomas
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Comparative studies with surface plasmon resonance and free oscillation rheometry on the inhibition of platelets with cytochalasin E and monoclonal antibodies towards GPIIb/IIIa2002Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 17, nr 9, s. 761-771Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In the haemostatic system a multitude of processes are intertwined in fine-tuned interactions that arrest bleeding, keep the circulatory system open, and the blood flowing. The occurrence of both surface and bulk interactions adds an additional dimension of complexity. These insights have led to the belief that global overall procedures can inform on the likely behaviour of the system in health and disease. Two sensing procedures: surface plasmon resonance (SPR), which senses surface interactions, and free oscillation rheometry (FOR), which senses interactions within the bulk, have been combined and evaluated. The contribution of blood cells, mainly platelets, to the SPR and FOR signals was explored by simultaneous SPR and FOR measurement during native whole blood coagulation, accelerated via the platelets through addition of SFLLRN peptide and inhibition of platelet aggregation with abciximab (ReoPro®) and of shape change with cytochalasin E. The SPR technique was found to be sensitive to inhibition of blood cell functions such as adhesion to and spreading on surfaces, as well as platelet aggregation. SPR seemed not to be directly sensitive to fibrin polymerisation in coagulating whole blood. The FOR technique detected the coagulation as a bulk phenomenon, i.e. the gelation of the blood due to fibrin formation was detected. The combination of SPR and FOR may therefore be suitable for studies on blood cell functions during coagulation.

  • 47.
    Hansson, Kenny
    et al.
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Tengvall, Pentti
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Rånby, Mats
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Lindahl, Tomas
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Surface plasmon resonance and free oscillation rheometry in combination: a useful approach for studies on haemostasis and interactions between whole blood and artificial surfaces2002Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 17, nr 9, s. 747-759Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In haemostatic and biomaterial research biological processes at surfaces and in the bulk phase of the surface-contacting medium are important. The present work demonstrates the usefulness of the combination of surface plasmon resonance (SPR), sensitive to changes in refractive index at surfaces, and free oscillation rheometry (FOR), sensitive to rheological properties of the bulk, for simultaneous real-time measurements on coagulation and fibrinolysis of blood plasma and coagulation of whole blood. SFLLRN stimulated coagulation of native whole blood presented a higher SPR signal with different appearance than plasma coagulation, while the FOR signals corresponding to plasma and whole blood coagulation were similar. This indicated that the SPR technique was more sensitive to cell-surface interactions than to fibrin formation in whole blood during coagulation, while the FOR technique were equally sensitive to coagulation in whole blood and plasma. Spontaneous coagulation of native whole blood in contact with methyl- and hydroxyl-terminated self-assembled monolayers (SAM) on gold and gold surfaces regenerated after coagulation were also studied. The regenerated gold surfaces displayed the shortest coagulation times, although the contact-activation of blood coagulation for these surfaces was low. The methylated and hydroxylated surfaces were comparable in terms of coagulation activation, while the hydroxylated surfaces presented FOR signals that indicated detaching of the coagulum from the surface. The combination of SPR and FOR is well suited for studies of cell– and protein–surface interactions and simultaneous bulk processes. Possible applications are investigations of blood cell defects in patients and monitoring of native whole blood interactions with artificial surfaces.

  • 48.
    Hansson, Kenny
    et al.
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Vikinge, T. P.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Hälsouniversitetet.
    Rånby, M.
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Tengvall, Pentti
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
    Johansen, Knut
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Hälsouniversitetet.
    Lindahl, Tomas
    Linköpings universitet, Institutionen för biomedicin och kirurgi, Klinisk kemi. Linköpings universitet, Hälsouniversitetet.
    Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay1999Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 14, nr 8-9, s. 671-682Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    It is previously shown that surface plasmon resonance (SPR) can be used to study blood plasma coagulation. This work explores the use of this technique for the analysis of tissue factor induced coagulation, i.e. prothrombin time (PT) analysis, of whole blood and plasma. The reference method was nephelometry. The prothrombin time analysis by SPR was performed by mixing two volumes of blood/plasma, one volume of thromboplastin, and one volume of CaCl2 solution directly on a sensor surface. The measurements show good agreement between nephelometry and SPR plasma analysis and also between SPR plasma and whole blood analysis. The effect of anticoagulant treatment on the clotting times was significant both quantitatively and qualitatively. The impact on the SPR signal of different physiological events in the coagulation process is discussed, and tentative interpretations of the sensorgram features are given. The major advantage of the SPR method compared to nephelometry is the possibility to perform analysis on whole blood instead of plasma. In conclusion, SPR is a promising method for whole blood coagulation analysis.

  • 49.
    Hao, Nanjing
    et al.
    Department of Chemistry, University of Massachusetts, USA.
    Neranon, Kitjanit
    KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi.
    Ramström, Olof
    KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi.
    Yan, Mingdi
    KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi. Department of Chemistry, University of Massachusetts, USA.
    Glyconanomaterials for biosensing applications2016Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 76, nr 15, s. 113-130Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Nanomaterials constitute a class of structures that have unique physiochemical properties and are excellent scaffolds for presenting carbohydrates, important biomolecules that mediate a wide variety of important biological events. The fabrication of carbohydrate-presenting nanomaterials, glyconanomaterials, is of high interest and utility, combining the features of nanoscale objects with biomolecular recognition. The structures can also produce strong multivalent effects, where the nanomaterial scaffold greatly enhances the relatively weak affinities of single carbohydrate ligands to the corresponding receptors, and effectively amplifies the carbohydrate-mediated interactions. Glyconanomaterials are thus an appealing platform for biosensing applications. In this review, we discuss the chemistry for conjugation of carbohydrates to nanomaterials, summarize strategies, and tabulate examples of applying glyconanomaterials in in vitro and in vivo sensing applications of proteins, microbes, and cells. The limitations and future perspectives of these emerging glyconanomaterials sensing systems are furthermore discussed.

  • 50.
    Hart, AL
    et al.
    CRANFIELD UNIV,CTR BIOTECHNOL,CRANFIELD MK43 0AL,BEDS,ENGLAND; HORTRES,BATSHELAR RES CTR,PALMERSTON NORTH,NEW ZEALAND; .
    Turner, APF
    Cranfield University, UK.
    Hopcroft, D
    CRANFIELD UNIV,CTR BIOTECHNOL,CRANFIELD MK43 0AL,BEDS,ENGLAND; HORTRES,BATSHELAR RES CTR,PALMERSTON NORTH,NEW ZEALAND; .
    On the use of screen- and ink-jet printing to produce amperometric enzyme electrodes for lactate1996Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 11, nr 3, s. 263-270Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Prototype electrochemical lactate electrodes based on lactate oxidase were produced by screen-printing and application of membranes by dip-coating or ink-jet printing. The link between enzyme and electrode was made by electro-deposition of platinum onto graphite pads. The linear range of the sensors was small; up to 2 mM lactate. Electrodes retained their activity when stored dry; activity remained high for 244 days. The properties of the electrodes indicate that screen- and ink-jet printing are feasible techniques for the machine production of lactate sensors.

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