Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Isotopic analyses of biological materials by single and multi-collector sector field ICP-MS
Luleå tekniska universitet.
2004 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Investigations concerning naturally occurring, mass dependent isotope fractionation of transition elements in biological materials have only been carried out during a very short period of time. Due to the lack of detailed descriptions of the analytical performance in previously reported work, the major focus of this study has therefore been to evaluate aspects of sample preparation and detection for obtaining precise and accurate isotopic ratios by multi-collector inductively coupled plasma mass spectrometry (MC- ICP-MS) using high mass-resolution. Quantitative determinations of elemental concentrations were performed by sector field ICP-MS using external calibration and internal standardization. Dissolution of whole blood and soft tissue reference materials in a micro-wave oven, or on a hot plate, using HNO3 resulted in concentrations of Zn, Fe and a host of other elements in agreement with certified values. This, together with high chemical yields of Zn and Fe (>93%) from the sample purification by anion- exchange chromatography or precipitation by NH3, limited the possibility for laboratory-induced isotope fractionation. Concentrations of the majority of potentially interfering elements were reduced to negligible levels, and most of the residual spectral interferences were resolved using medium (in the case of Zn) or high (Fe) mass-resolution settings of the MC-ICP-MS-instrument. To correct for differences in instrumental mass discrimination effects between samples and isotopic standards, concentrations were matched to within 10-20%, and Cu and Ni were employed as elemental spikes, for measurements of Zn and Fe, respectively. Almost all samples and standards were analysed in duplicate (or triplicate), and exponential correction for instrumental mass discrimination was performed by applying the exponential model. Precision at the sub-‰ level was achieved for both Zn and Fe delta-values, relative to a JMC Zn standard and an IRMM-014 Fe isotopic reference material, respectively. For the investigated reference materials, delta66/64Zn ranged from 0.07 ± 0.03‰ in human hair to 0.50 ± 0.03‰ in bovine muscle. Concerning Fe, a delta56/54Fe-value of -2.83 ± 0.06‰ was obtained for a human whole blood reference material. Variations in delta66/64Zn values, ranging from +0.56‰ in human whole blood to -0.60‰ in human hair were found, demonstrating fractionation of the Zn pool between body compartments. Where comparable isotopic determinations exist, the results were in good agreement.

Place, publisher, year, edition, pages
Luleå: Luleå tekniska universitet, 2004. , p. 15
Series
Licentiate thesis / Luleå University of Technology, ISSN 1402-1757 ; 2004:11
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
URN: urn:nbn:se:ltu:diva-26146Local ID: cdd04b60-ae48-11db-879f-000ea68e967bOAI: oai:DiVA.org:ltu-26146DiVA, id: diva2:999305
Note
Godkänd; 2004; 20070127 (ysko)Available from: 2016-09-30 Created: 2016-09-30Bibliographically approved

Open Access in DiVA

fulltext(35728 kB)36 downloads
File information
File name FULLTEXT01.pdfFile size 35728 kBChecksum SHA-512
88084c1e1fb03d05120b384d1773055c7006b4f93b5e56691013930276b9df2699a3f4a2d541d556092a51fc02864304d29d01eff2fa4cb781458ca3bf523415
Type fulltextMimetype application/pdf

Geochemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 36 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 20 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf