Conformational transitions of the phosphodiester backbone in native DNA: two-dimensional magic-angle-spinning 31P-NMR of DNA fibers
1997 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 73, no 3, 1539-1552 p.Article in journal (Refereed) Published
Solid-state 31P-NMR is used to investigate the orientation of the phosphodiester backbone in NaDNA-, LiDNA-, MgDNA-, and NaDNA-netropsin fibers. The results for A- and B-DNA agree with previous interpretations. We verify that the binding of netropsin to NaDNA stabilizes the B form, and find that in NaDNA, most of the phosphate groups adopt a conformation typical of the A form, although there are minor components with phosphate orientations close to the B form. For LiDNA and MgDNA samples, on the other hand, we find phosphate conformations that are in variance with previous models. These samples display x-ray diffraction patterns that correspond to C-DNA. However, we find two distinct phosphate orientations in these samples, one resembling that in B-DNA, and one displaying a twist of the PO4 groups about the O3-P-O4 bisectors. The latter conformation is not in accordance with previous models of C-DNA structure.
Place, publisher, year, edition, pages
1997. Vol. 73, no 3, 1539-1552 p.
Research subject Chemistry of Interfaces
IdentifiersURN: urn:nbn:se:ltu:diva-12234Local ID: b55d5720-ecc4-11db-bc0c-000ea68e967bOAI: oai:DiVA.org:ltu-12234DiVA: diva2:985184
Upprättat; 1997; 20070417 (bajo)2016-09-292016-09-29