Change search
ReferencesLink to record
Permanent link

Direct link
Effects of Persistent Organic Pollutants and Their Mixtures on Biotransformation and Oxidative Stress in Zebrafish Embryo
Örebro University, School of Science and Technology, Örebro University, Sweden.
2016 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Persistent organic pollutants (POPs) cause significant effects on organisms due to their resistance to environmental degradation and specific toxic responses. Although POPs toxicities were linked to oxidative stress in the previous studies, there are few POPs studies that link them with oxidative stress in zebrafish during development. Aim of this study is to investigate effect of selected POPs on expression of genes involved in oxidative stress response and biotransformation of xenobiotics in zebrafish. Zebrafish embryos in 96 hours’ post-fertilization were exposed to selected POPs and their mixture. To explore the developmental toxicity in zebrafish early stage, we exposed 3,3',4,4',5-pentachlorobiphenyl (PCB), perfluorooctanesulfonic acid (PFOS) and perfluorohexanoic acid (PFHxA) at concentrations of 7.5 μg/L, 50 μM, 50 μM until 96 hours’ post-fertilization. The effects were measured by gene expression quantification technique - quantitative real-time PCR (qPCR). Significant up-regulation in gene expression was detected in embryos treated with mixture of PCB with PFHxA and PCB with PFOS only for cytochrome P450(cyp1a). The results also showed the treatment with selected compound caused significant higher upregulation of cyp1a when we compare the treatment in the individual compounds to the mixture compounds. However, treatments did not cause changes in expression of genes involved in oxidative stress response (glutathione peroxidase 1a(gpx1a), tumor protein p53(tp53), aryl hydrocarbon receptor 2 (ahr2)). The result also suggests that exposure to selected POPs in mixtures or alone is not causing oxidative stress in early stage of embryonal development of zebrafish but activating biotransformation function of the organism. Effect of activation of biotransformation capacity by means of cyp1a upregulation is also higher when POPs are in mixtures over when used as individual substance.

Place, publisher, year, edition, pages
Keyword [en]
Zebrafish, Developmental toxicity; Mixture toxicity; Oxidative stress
National Category
Biological Sciences
URN: urn:nbn:se:oru:diva-52495OAI: diva2:974057
Subject / course
Available from: 2016-09-23 Created: 2016-09-23 Last updated: 2016-09-23Bibliographically approved

Open Access in DiVA

fulltext(314 kB)4 downloads
File information
File name FULLTEXT01.pdfFile size 314 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
School of Science and Technology, Örebro University, Sweden
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 4 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 2 hits
ReferencesLink to record
Permanent link

Direct link