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Metodjämförelse mellan DiffMaster Octavia och CellaVision DM1200 avseende differentialräkning av leukocyter: en viktig analys inom vården
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
2016 (Swedish)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [sv]

Leukocyter, vita blodkroppar, utgör en del av kroppens immunförsvar. De utmognar från hematopoetiska stamceller i benmärgen. Leukocyterna kan delas in i neutrofila granulocyter, lymfocyter, monocyter, eosinofila granulocyter och basofila granulocyter. För att bestämma antalet leukocyter i blodet analyseras leukocytpartikelkoncentration (B-LPK) med cellräknare. Om B-LPK är förhöjt eller sänkt utförs en differentialräkning av leukocyter (B-Diff) för att se i vilket cellsystem förändring föreligger. Den manuella analysen utförs på perifera blodutstryk färgade med cytokemisk färg, May-Grünwald Giemsa. Utstryket granskas i ett automatiskt mikroskopiskt system som räknar, fotograferar och förklassificerar leukocyter efter deras utseende. DiffMaster Octavia och CellaVision DM1200 är två varianter av ett sådant instrument från samma tillverkare (CellaVision AB, Lund, Sverige). Syftet med studien var att utföra en metodjämförelse mellan dessa instrument genom analys av 60 patientprov bestående av venöst blod taget i EDTA-rör. Proven samlades in slumpmässigt från patienter (32 män och 28 kvinnor) mellan 19-95 år. Resultaten visade enligt tvåsidigt parat t-test ingen signifikant skillnad avseende differentialräkning av neutrofila granulocyter, lymfocyter och monocyter. Korrelationen var 0,95, 0,91 respektive 0,68. Det fanns dock en signifikant skillnad mellan instrumenten avseende differentialräkning av eosinofila- och basofila granulocyter, korrelationen var 0,91 respektive 0,20. När endast 200 celler räknas kan ett resultat på 2 % skilja upp till 1-5 %. Därför blir skillnader på leukocyttyper som utgör enstaka procent i blodet normalt mycket stora. Metodjämförelsen visade att båda instrumenten ger samma resultat och anses därför likvärdiga vid analys av manuell B-Diff.

Abstract [en]

Leukocytes, white blood cells, are cells of the immune system. They are produced and derived from hematopoietic stem cells in the bone marrow. Leukocytes can be divided into neutrophils, lymphocytes, monocytes, eosinophils and basophils. To determine the numbers of leukocytes in blood the leukocyte particle concentration (B-LPK) can be analyzed by cell counters. When B-LPK is elevated or lowered a differential count of leukocytes (B-Diff) is performed to see in which cell systems the change exists. The manual analysis involves peripheral blood smears stained with a cytochemical color, May-Grünwald Giemsa. The smear examined in an automatic microscopically system that counts, photographs and pre-classify leukocytes by its appearance. DiffMaster Octavia and CellaVision DM1200 are two variants of such instruments from the same manufacturer (CellaVision AB, Lund, Sweden). The aim of the study was to do a comparison between these instruments by analyzing 60 samples consisting venous blood in EDTA-tubes. The samples were collected randomly from patients (32 men and 28 women) between 19-95 years old. The results from two-sided paired t-test showed no significant difference between the differential count of neutrophils, lymphocytes and monocytes. The correlation was 0,95, 0,91 and 0,68. However, there was a significant difference between the instruments differential count of eosinophils and basophils, the correlation was 0,91 and 0,20. When counting only 200 cells a profit of 2 % distinguish up to 1-5 %. Abnormalities in leukocytes which represents only a few percent in blood can therefore be very large. Method comparison showed that both instruments give the same results and are considered equivalent in analysis of manual B-Diff.

Place, publisher, year, edition, pages
2016. , 35 p.
Keyword [en]
DiffMaster Octavia, CellaVision DM1200
Keyword [sv]
Leukocyter, B-Diff, Metodjämförelse, DiffMaster Octavia, CellaVision DM1200
National Category
Hematology
Identifiers
URN: urn:nbn:se:lnu:diva-56366OAI: oai:DiVA.org:lnu-56366DiVA: diva2:958039
External cooperation
Avdelningen för Klinisk kemi på Blekingesjukhuset i Karlskrona
Subject / course
Biomedical Laboratory Science; Biomedical Laboratory Science
Educational program
Biomedical Laboratory Science Programme, 180 credits; Biomedical Laboratory Science Programme, 180 credits
Supervisors
Examiners
Available from: 2016-09-06 Created: 2016-09-05 Last updated: 2016-09-06Bibliographically approved

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Ölje, Elin
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