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Detection of Campylobacter in human and animal field samples in Cambodia
Swedish Univ Agr Sci, Div Reprod, Dept Clin Sci, Box 7054, SE-75007 Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine.
Natl Inst Publ Hlth, Phnom Penh, Cambodia..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
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2016 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, no 6, 508-515 p.Article in journal (Refereed) Published
Abstract [en]

Campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with Campylobacter jejuni and C. coli being the most commonly detected species. The aim of this study was to detect Campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex PCR in faecal samples frozen before analysis. Of 681 human samples, 82 (12%) tested positive by PCR (C. jejuni in 66 samples and C. coli in 16), but none by routine culture. Children were more commonly Campylobacter positive (19%) than adult males (8%) and females (7%). Of 853 livestock samples, 106 (12%) tested positive by routine culture and 352 (41%) by PCR. Campylobacter jejuni was more frequent in chickens and ducks and C. coli in pigs. In conclusion, Campylobacter proved to be highly prevalent by PCR in children (19%), ducks (24%), chickens (56%) and pigs (72%). Routine culturing was insufficiently sensitive in detecting Campylobacter in field samples frozen before analysis. These findings suggest that PCR should be the preferred diagnostic method for detection of Campylobacter in humans and livestock where timely culture is not feasible.

Place, publisher, year, edition, pages
2016. Vol. 124, no 6, 508-515 p.
Keyword [en]
Campylobacter, Cambodia, humans, livestock, zoonosis
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-302222DOI: 10.1111/apm.12531ISI: 000376602800010PubMedID: 26991032OAI: oai:DiVA.org:uu-302222DiVA: diva2:957153
Funder
Swedish Civil Contingencies AgencySida - Swedish International Development Cooperation AgencySwedish Research Council Formas
Available from: 2016-09-01 Created: 2016-08-31 Last updated: 2016-09-01Bibliographically approved

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Tano, EvaEllström, PatrikRautelin, Hilpi
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Clinical Microbiology and Infectious MedicineDepartment of Medical SciencesDepartment of Medical Biochemistry and Microbiology
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