Change search
ReferencesLink to record
Permanent link

Direct link
Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)
Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).
University of Gothenburg.
Kristianstad University, School of Education and Environment, Avdelningen för Naturvetenskap. Kristianstad University, Research environment Man & Biosphere Health (MABH).ORCID iD: 0000-0003-4190-5431
Uppsala University.
Show others and affiliations
2016 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 55, 452-459 p.Article in journal (Refereed) Published
Abstract [en]

Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from ∼8.1 to ∼7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus, V. tubiashii, V. splendidus, V. alginolyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by ∼65–90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5–3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens.

Place, publisher, year, edition, pages
2016. Vol. 55, 452-459 p.
Keyword [en]
Ocean acidification, Mytilus edulis, Antimicrobial peptide, Gill tissue, Vibrio, LPS, Proteome analysis, LC-Orbitrap MS/MS analysis
National Category
Immunology
Identifiers
URN: urn:nbn:se:hkr:diva-15677DOI: 10.1016/j.fsi.2016.04.007PubMedID: 27288994OAI: oai:DiVA.org:hkr-15677DiVA: diva2:952008
Funder
Swedish Research Council, 621-2011-4423Swedish Research Council, 2015-4870 JB
Available from: 2016-08-11 Created: 2016-08-11 Last updated: 2016-08-11Bibliographically approved

Open Access in DiVA

fulltext(1024 kB)18 downloads
File information
File name FULLTEXT01.pdfFile size 1024 kBChecksum SHA-512
e60f481a0f4c7343cb63bb8d627d55d8017a492066e292be93a300855e516e3626b3e95b3ae465e62417dc2e27e82164935b27b209f4487d4d7aba5c28377a28
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hernroth, BodilTassidis, Helena
By organisation
Avdelningen för NaturvetenskapResearch environment Man & Biosphere Health (MABH)
In the same journal
Fish and Shellfish Immunology
Immunology

Search outside of DiVA

GoogleGoogle Scholar
Total: 18 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 10 hits
ReferencesLink to record
Permanent link

Direct link