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Adipokines and Vascular Endothelial Growth Factor in Normal Human Breast Tissue in Vivo - Correlations and Attenuation by Dietary Flaxseed
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center of Paediatrics and Gynaecology and Obstetrics, Department of Gynaecology and Obstetrics in Linköping.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Oncology.
2016 (English)In: Journal of mammary gland biology and neoplasia, ISSN 1083-3021, E-ISSN 1573-7039, Vol. 21, no 1-2, 69-76 p.Article in journal (Refereed) PublishedText
Abstract [en]

Exposure to sex steroids increases the risk of breast cancer but the exact mechanisms are yet to be elucidated. Events in the microenvironment are important for carcinogenesis. Diet containing phytoestrogens can affect the breast microenvironment and alter the risk of breast cancer. It has previously been shown that estrogen regulates extracellular levels of leptin, adiponectin, and VEGF in normal breast tissue in vivo. Whether these proteins correlate in breast tissue in vivo or if diet addition of flaxseed, a major source of phytoestrogens in Western diets, alters adipokine levels in breast tissue are unknown. We used microdialysis to sample proteins of normal human breast tissue and abdominal subcutaneous fat in situ in 34 pre-and postmenopausal women. In vitro, co-culture of breast cancer cells and primary human adipocytes was used. In vivo, in normal breast tissue, a significant positive correlation between VEGF and leptin was detected. No correlations were found in fat tissue. Co-culture of adipocytes and breast cancer cells per se increased the secretion of VEGF and leptin and enhanced the effects of estradiol compared to culture of either cell type alone. In vitro, inhibition of VEGF diminished the release of leptin while inhibition of leptin had no influence on VEGF secretion. The levels of leptin decreased and adiponectin increased after a dietary addition of 25 g of flaxseed/day for one menstrual cycle. We conclude that VEGF and leptin correlate significantly in normal human breast tissue in vivo and that dietary addition of flaxseed affect adipokine levels in the breast.

Place, publisher, year, edition, pages
SPRINGER/PLENUM PUBLISHERS , 2016. Vol. 21, no 1-2, 69-76 p.
Keyword [en]
Flaxseed; Diet; Microdialysis; Estrogen; Leptin; Adiponectin
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:liu:diva-130433DOI: 10.1007/s10911-016-9352-9ISI: 000379327000009PubMedID: 27059487OAI: oai:DiVA.org:liu-130433DiVA: diva2:951178
Available from: 2016-08-07 Created: 2016-08-05 Last updated: 2016-08-22

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Morad, VivianAbrahamsson, AnnelieKjölhede, PrebenDabrosin, Charlotta
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Department of Physics, Chemistry and BiologyFaculty of Science & EngineeringDivision of Clinical SciencesFaculty of Medicine and Health SciencesDepartment of Gynaecology and Obstetrics in LinköpingDepartment of Oncology
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Journal of mammary gland biology and neoplasia
Cancer and Oncology

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