Identification of a butanol tolerant Saccharomyces cerevisiae strain and of a gene associated with enhanced butanol tolerance.
Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
The most widely used biofuel on the market today is ethanol derived from food crops, such as maize and sugarcane. Ethanol is renewable and environmental friendly but the low energy density makes it unable to compete with fossil fuels. Enlarged focus on replacing fossil fuels with biofuels from renewable biomass have identified isobutanol and 1-butanol as future biofuels, possessing similar capabilities as gasoline e.g. high octane number and energy density. The yeast Saccharomyces cerevisiae can produce butanol through fermentation of carbohydrates but butanol concentrations over 2% is toxic to most strains. To reach the commercial requirements for economic and efficient isobutanol production using S. cerevisiae, higher butanol tolerance is crucial.
The butanol tolerance of isolated strains of S. cerevisiae from different habitats were examined using spot plating and growth measurements. The results showed variance in butanol tolerance between strains, where the most tolerant strains were able to grow in isobutanol concentration up to 3 %. The expression of genes associated with increased butanol tolerance was investigated by Quantitative Real-time PCR. Data showed an upregulation of RPN4 in strains subjected to butanol induced stress. The study aims to identify butanol tolerant strains that can be engineered for efficient butanol production for sustainable biofuel production.
Place, publisher, year, edition, pages
2016. , 24 p.
Saccharomyces cerevisiae, Isobutanol, Spot plating, Renewable biofuel
IdentifiersURN: urn:nbn:se:liu:diva-130346ISRN: LITH-IFM-G-EX--16/3185--SEOAI: oai:DiVA.org:liu-130346DiVA: diva2:950446
Subject / course