Purification and characterization of a pentameric ligand gated ion channel: GLIC
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Pentameric ligand gated ion channels are important players in the nervous system due to their
role as converters of chemical signals into electrical responses. The malfunction of these
important ion channels has been proven to be involved in a variety of neurological diseases.
Recently, new crystal structures as well as electron microscopy structures have started to
emerge, providing society with new information and coupling structure to function. However,
the necessity of studying this important group of ion channels in their natural environment –
membranes – is high and thus are the recently emerged structures a bit limited. The project
aimed to characterize GLIC’s conducting properties with a port-a-patch setup. This was not
achieved but initial experiments were performed. However, the purification process of GLIC
was successful and optimized during the project. A Histidine-tagged protein with a mass
around 37 kDa was observed after western blot, possibly corresponding to GLIC.
Place, publisher, year, edition, pages
2016. , 45 p.
UPTEC X, 16 023
pentarmeric ligand gated ion channels, GLIC, port-a-patch electrophysiology methodology, IMAC, SDS-page, western blot
Natural Sciences Biochemistry and Molecular Biology Biophysics
IdentifiersURN: urn:nbn:se:uu:diva-298352OAI: oai:DiVA.org:uu-298352DiVA: diva2:945830
Science for Life Laboratory
Molecular Biotechnology Engineering Programme
Andersson, Magnus, PhD
Andersson, Jan, UniversitetslektorHenriksson, Lena, PhDSelmer, Maria