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Purification and characterization of a pentameric ligand gated ion channel: GLIC
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Pentameric ligand gated ion channels are important players in the nervous system due to their

role as converters of chemical signals into electrical responses. The malfunction of these

important ion channels has been proven to be involved in a variety of neurological diseases.

Recently, new crystal structures as well as electron microscopy structures have started to

emerge, providing society with new information and coupling structure to function. However,

the necessity of studying this important group of ion channels in their natural environment –

membranes – is high and thus are the recently emerged structures a bit limited. The project

aimed to characterize GLIC’s conducting properties with a port-a-patch setup. This was not

achieved but initial experiments were performed. However, the purification process of GLIC

was successful and optimized during the project. A Histidine-tagged protein with a mass

around 37 kDa was observed after western blot, possibly corresponding to GLIC.

Place, publisher, year, edition, pages
2016. , 45 p.
Series
UPTEC X, 16 023
Keyword [en]
pentarmeric ligand gated ion channels, GLIC, port-a-patch electrophysiology methodology, IMAC, SDS-page, western blot
National Category
Natural Sciences Biochemistry and Molecular Biology Biophysics
Identifiers
URN: urn:nbn:se:uu:diva-298352OAI: oai:DiVA.org:uu-298352DiVA: diva2:945830
External cooperation
Science for Life Laboratory
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2016-07-05 Created: 2016-07-03 Last updated: 2016-07-05Bibliographically approved

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CiteExportLink to record
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