Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Metodutveckling av en vätskebaserad cytologisk metod vid preparering av exsudat: En jämförelse med konventionell cytologi
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2016 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Method development of a liquid-based cytological method using exudate : – a comparison with conventional method (English)
Abstract [sv]

Två huvudsakliga metodprinciper används inom cytologi för diagnostisering av cellförändringar, nämligen konventionell och vätskebaserad metod. De kan båda appliceras på såväl gynekologiska som icke-gynekologiska prover, där den senare bland annat omfattar olika sorters exsudat. Syftet med den här studien var att utveckla metoden för den vätskebaserade metoden så att etanolfixerade exsudat kunde prepareras och även påvisa bättre resultat än då de preparerats med konventionell metod. För att göra detta har 61 unika prover kategoriserade som exsudat preparerats totalt, varav 61 med konventionell metod, 54 med vätskebaserad metod och 22 med vätskebaserad metod med tillsats av ättiksyra. De färdiga glasen bedömdes sedan i mikroskop och gavs scorevärden utifrån fyra parametrar: mängden celler exklusive inflammatoriska celler; bedömbarheten av cellmorfologin; mängden inflammatorisk komponent samt mängden bakgrundsmaterial. Resultaten visade ingen förbättring mellan de glas som preparerats med konventionell eller vätskebaserad metod. Däremot visade resultaten för de ättiksyrabehandlade proverna på förbättrade scorevärden jämfört med de andra metoderna.

Som slutsats drogs att vätskebaserad metod med tillsats av ättiksyra uppnår syftet eftersom det reducerar mängden bakgrundsmaterial, förekomst av ring på objektglasen samt vidhåller en god cellmorfologi, vilket gör proverna lättare att diagnostisera för cytodiagnostikerna.

Abstract [en]

Two main principles is used within cytology in order to diagnose cytological abnormalities; conventional and liquid-based cytology. Both methods can be applied on both gynaecological and non-gynaecological samples of which the later includes samples categorized as exudate. The aim of this study was to develop the method for liquid-based cytology so that exudate fixated with ethanol could be prepared and also achieve better results compared to conventional method. In order to do so, 61 unique samples were prepared of which 61 with conventional method, 54 with liquid-based method and 22 with liquid-based method with added acetic acid. The slides was then examined in microscope and was given score values within four parameters: amount of cells; cell morphology; amount of inflammatory component and amount of background. The results indicated no difference between the slides prepared with conventional or liquid-based method. However, the slides prepared with addition of acetic acid indicated more opportunistic score values when compared.

The conclusion was that liquid-based method with the addition of acetic acid did satisfy the aim of this study as it reduces the amount of background, reduces “ring formation” on the slides and preserve the cells morphology well, which makes the samples easier to diagnose.

Place, publisher, year, edition, pages
2016. , 33 p.
Keyword [en]
Exudate; Conventional cytology; Liquid-based cytology; Acetic acid
Keyword [sv]
Exsudat; Konventionell cytologi; Vätskebaserad cytologi; Ättiksyra
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-31010ISRN: JU-HHJ-BLA-1-20160030OAI: oai:DiVA.org:hj-31010DiVA: diva2:945582
Subject / course
HHJ, Biomedical Laboratory Science
Presentation
2016-06-07, Hälsohögskolan i Jönköping, 11:00 (Swedish)
Supervisors
Examiners
Available from: 2016-07-05 Created: 2016-07-01 Last updated: 2016-07-05Bibliographically approved

Open Access in DiVA

fulltext(745 kB)49 downloads
File information
File name FULLTEXT01.pdfFile size 745 kBChecksum SHA-512
57b4d6a3bcc15b66c2bbc85ce37b77555ebf01af854960b5b8e701219929c021dd6416b44658a9994152a228cb27f08611b1778e3e85a64ef31a2b38ab278966
Type fulltextMimetype application/pdf

By organisation
HHJ, Dep. of Natural Science and Biomedicine
Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 49 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 62 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf