Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Proofreading neutralizes potential error hotspots in genetic code translation by transfer RNAs
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
2016 (English)In: RNA: A publication of the RNA Society, ISSN 1355-8382, E-ISSN 1469-9001, Vol. 22, no 6, 896-904 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

The ribosome uses initial and proofreading selection of aminoacyl-tRNAs for accurate protein synthesis. Anomalously high initial misreading in vitro of near-cognate codons by tRNAHis and tRNAGlu suggested potential error hotspots in protein synthesis, but in vivo data suggested their partial neutralization. To clarify the role of proofreading in this error reduction, we varied the Mg2+ ion concentration to calibrate the total accuracy of our cell-free system to that in the living Escherichia coli cell. We found the total accuracy of tRNA selection in our system to vary by five orders of magnitude depending on tRNA identity, type of mismatch, and mismatched codon position. Proofreading and initial selection were positively correlated at high, but uncorrelated at low initial selection, suggesting hyperactivated proofreading as a means to neutralize potentially disastrous initial selection errors.

Place, publisher, year, edition, pages
2016. Vol. 22, no 6, 896-904 p.
Keyword [en]
ribosome, genetic code, accuracy, tRNA selection, proofreading, error hotspots
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-298078DOI: 10.1261/rna.055632.115ISI: 000376205600008PubMedID: 27090284OAI: oai:DiVA.org:uu-298078DiVA: diva2:944813
Funder
Knut and Alice Wallenberg FoundationSwedish Research Council
Available from: 2016-06-30 Created: 2016-06-29 Last updated: 2017-11-28Bibliographically approved

Open Access in DiVA

fulltext(710 kB)219 downloads
File information
File name FULLTEXT01.pdfFile size 710 kBChecksum SHA-512
d45f6fdff13df72dcf0851d5d96144bbd099881caa7d7cda9127ea74889155826062f17875430f378c40950405cf3166df8381b305ba2021ad018cf2e8df9ce8
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Zhang, JingjiIeong, Ka-WengEhrenberg, Måns
By organisation
Structure and Molecular Biology
In the same journal
RNA: A publication of the RNA Society
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 219 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 671 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf