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Placenta growth factor som biomarkör vid screening av preeklampsi: Litteraturfördjupning och verifiering av metodologi
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2016 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Placenta growth factor as a biomarker for screening of preeclampsia : A literature recess and verification of methodology (English)
Abstract [sv]

Under år 2003-2009 utgjorde hypertensiva sjukdomar, såsom eklampsi och preeklampsi, 14,0% av värdens mödradödlighet. Preeklampsi kännetecknas vanligtvis av kliniska observationer av hypertoni och signifikant proteinuri i graviditetens andra trimester. Inom diagnostiken används en riskbedömningsprogramvara som kan beräkna vilken sannolikhetsgrad den havande kvinnan har för att utveckla preeklampsi. Förutom mätning av blodtryck och proteinuri har biomarkören placenta growth factor 1 (PlGF-1) visat ett högt prediktivt värde vid bedömningen. Studien syftade till att kartlägga och fördjupa sig i metoderna som analyserar biomarkören samt verifiera metoden för PlGF på instrumentet Brahms Kryptor compact plus. Fördjupningen baserades på granskning av vetenskapliga artiklar och resulterade i två manuella och tre automatiserade metoder. Metoden Quantikine användes i 47% av artiklarna och konstaterades som studiens golden standard. Vid jämförelse av metoderna sågs en lägre bakgrundsstörning, en högre sensitivitet samt en kortare analystid hos de automatiserade metoderna. Den laborativa verifieringen innefattade bestämning av överensstämmelse med externt laboratorium, beräkning av instrumentets provsmitta mellan höga och låga prov samt kvantifiering av inomserie- och mellanliggande precision. Verifieringen resulterade i en god överensstämmelse (r=0,953, p=0,327) med det externa laboratoriet, en konstaterad provsmitta på 0,04% samt en god precision inom leverantörens angivelser.

Abstract [en]

Between 2003-2009 hypertensive disorders as eclampsia and preeclampsia constituted 14.0% of the world’s maternal mortality. Preeclampsia characterize as clinical observations of hypertension and significant proteinuria in the second trimester of pregnancy. In diagnostics a risk assessment software is normally used to estimate the probability of developing the disorder. Besides calculating the blood pressure and proteinuria, the placenta growth factor 1 (PlGF-1) has proven to possess a high predictive value. The study’s aim was to chart the different methods used to quantify the biomarker and verify the method for PlGF on Brahms Kryptor compact plus. The recess was based on review of scientific articles and resulted in the findings of two manual and three automated methods. The method Quantikine was used in 47% of the articles and was seen as the golden standard of the study. When comparing the methods a lower signal to noise-ratio, a higher sensitivity and a shorter assay time was observed in the automated methods. The verification contained determination of compliance with an external laboratory, calculation of carry over and quantification of inter-assay and intra-assay precision. The verification resulted in a good compliance (r=0.953, p=0,327) with the external laboratory, a carry over at 0,04% and a good precision within the providers indication.

Place, publisher, year, edition, pages
2016. , 31 p.
Keyword [en]
Placenta growth factor, Preeclampsia, Screening, Verification, Methodology
Keyword [sv]
Placenta growth factor, Preeklampsi, Screening, Verifiering, Metodanalys
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-30964ISRN: JU-HHJ-BLA-1-20160029OAI: oai:DiVA.org:hj-30964DiVA: diva2:944730
Subject / course
HHJ, Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2016-07-04 Created: 2016-06-29 Last updated: 2016-07-04Bibliographically approved

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Placenta growth factor som biomarkör vid screening av preeklampsi: Litteraturfördjupning och verifiering av metodologi(1014 kB)123 downloads
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