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Can Sterol Carrie Protein-2 function as a solubility tag in E.coli?
Linköping University, Department of Physics, Chemistry and Biology, Biology.
2016 (English)Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
Abstract [en]

Expressing foreign proteins in E.coli is a major challenge because they often tend to develop into unsolvable and inactive proteins. They aggregate into so called  inclusion bodies which prevent expression of the protein. This problem might be avoided by fusing the gene of the foreign protein with a soluble protein called solubility tags, which  function is to enhance the solubility of the foreign protein. This report investigates whether Sterol Carrier Protein-2 (SCP-2) could function as a solubility tag. The experiment was carried out by fusing SCP-2 to two recombinant proteins, Green fluorescent protein (GFP) and a form of chloroamphenicol acetyl transferase (CATΔ9). The gene fusion was then inserted into a pET-15 vector and transformed into  the E.coli strain BL21(DE3) to be expressed. The results obtained from Western blot and PageBlue staining indicates that SCP-2 does not enhance the solubility of GFP or CATΔ9 since neither of them was expressed.  Furthermore, previous studies have shown that GFP can in fact be expressed  usingmaltose binding protein (MBP) as a solubility tag. Unfortunately, no success has been made regarding CATΔ9. In conclusion, regarding the results from this report, SCP-2 does not function as a solubility tag. However, further studies should be carried out on SCP-2 with more experiments before rejecting the possibility to use SCP-2 as a solubility tag.

Place, publisher, year, edition, pages
2016. , 20 p.
Keyword [en]
CATΔ9, E.coli, GFP, pET-15b, protein expression, recombinant proteins, SCP-2, Solubility tag
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:liu:diva-129803ISRN: LITH-IFM-G-EX—16/3193--SEOAI: diva2:943969
External cooperation
Isak Mikkola
Subject / course
Available from: 2016-07-14 Created: 2016-06-28 Last updated: 2016-07-14Bibliographically approved

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