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Distribution and Molecular Characterization of Human Adenovirus and Epstein-Barr Virus Infections in Tonsillar Lymphocytes Isolated from Patients Diagnosed with Tonsillar Diseases
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infection medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Otolaryngology and Head and Neck Surgery.
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2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 5, article id e0154814Article in journal (Refereed) Published
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Abstract [en]

Surgically removed palatine tonsils provide a conveniently accessible source of T and B lymphocytes to study the interplay between foreign pathogens and the host immune system. In this study we have characterised the distribution of human adenovirus (HAdV), Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) in purified tonsillar T and B cell-enriched fractions isolated from three patient age groups diagnosed with tonsillar hypertrophy and chronic/recurrent tonsillitis. HAdV DNA was detected in 93 out of 111 patients (84%), while EBV DNA was detected in 58 patients (52%). The most abundant adenovirus type was HAdV-5 (68%). None of the patients were positive for HCMV. Furthermore, 43 patients (39%) showed a co-infection of HAdV and EBV. The majority of young patients diagnosed with tonsillar hypertrophy were positive for HAdV, whereas all adult patients diagnosed with chronic/recurrent tonsillitis were positive for either HAdV or EBV. Most of the tonsils from patients diagnosed with either tonsillar hypertrophy or chronic/recurrent tonsillitis showed a higher HAdV DNA copy number in T compared to B cell-enriched fraction. Interestingly, in the majority of the tonsils from patients with chronic/recurrent tonsillitis HAdV DNA was detected in T cells only, whereas hypertrophic tonsils demonstrated HAdV DNA in both T and B cell-enriched fractions. In contrast, the majority of EBV positive tonsils revealed a preference for EBV DNA accumulation in the B cell-enriched fraction compared to T cell fraction irrespective of the patients' age.

Place, publisher, year, edition, pages
2016. Vol. 11, no 5, article id e0154814
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Surgery Otorhinolaryngology Cancer and Oncology
Identifiers
URN: urn:nbn:se:uu:diva-297795DOI: 10.1371/journal.pone.0154814ISI: 000375674800050PubMedID: 27136093OAI: oai:DiVA.org:uu-297795DiVA, id: diva2:943536
Funder
Swedish Cancer Society, 12 0504Swedish Cancer Society, 13 0469Swedish Research Council, K2012-99X-21959-01-3Swedish Research Council, 2006-5038-36531-16Åke Wiberg Foundation
Note

De 2 sista författarna delar sistaförfattarskapet.

Available from: 2016-06-28 Created: 2016-06-28 Last updated: 2017-11-28Bibliographically approved

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Assadian, FarzanehSandström, KarlBondeson, KåreLaurell, GöranLidian, AdnanSvensson, CatharinaAkusjärvi, GöranBergqvist, AndersPunga, Tanel
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Department of Medical Biochemistry and MicrobiologyOtolaryngology and Head and Neck SurgeryInfection medicineDepartment of Medical Sciences
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