Promoter regulation: designing cells for biotechnological applications
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
The filamentous cyanobacteria Nostoc punctiforme ATCC 29133 is a model species fordevelopment of sustainable production methods of numerous compounds. One of its uniquefeatures is the anaerobic environment of the strains nitrogen fixing heterocyst cells. To be ableto properly utilize this environment, more knowledge regarding what regulates cell specificexpression is required. In this study, three motifs of the NsiR I promoter of Anabaena sp.PCC 7120 was studied in this system utilizing YFP-fluorescence as a reporter to determinetheir impact on spatial expression pattern. Investigations were performed on immobilizedcells with the use of confocal microscopy and results point towards sigma factor regulation.
Place, publisher, year, edition, pages
2016. , 66 p.
UPTEC X, 16 021
Cyanobacteria, heterocyst, promoter, specificity, confocal microscopy, immobilization
IdentifiersURN: urn:nbn:se:uu:diva-297502OAI: oai:DiVA.org:uu-297502DiVA: diva2:942126
Molecular Biotechnology Engineering Programme
2016-06-03, C2:305, BMC, Husargatan 3, Uppsala, 15:15 (English)
Stensjö, Karin, PhD
Krabbe, Margareta, PhDHenriksson, Lena, PhDLindberg, Pia