Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Pronounced peptide selectivity for melanoma through tryptophan end-tagging
Nanyang Technol Univ, Lee Kong Chian Sch Med, 11 Mandalay Rd, Singapore 308232, Singapore.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy. Univ Tehran, Inst Biochem & Biophys, Peptide Chem Lab, Tehran 1417614335, Iran..
Nanyang Technol Univ, Lee Kong Chian Sch Med, 11 Mandalay Rd, Singapore 308232, Singapore..
Show others and affiliations
2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 24952Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Effects of oligotryptophan end-tagging on the uptake of arginine-rich peptides into melanoma cells was investigated under various conditions and compared to that into non-malignant keratinocytes, fibroblasts, and erythrocytes, also monitoring resulting cell toxicity. In parallel, biophysical studies on peptide binding to, and destabilization of, model lipid membranes provided mechanistic insight into the origin of the selectivity between melanoma and non-malignant cells. Collectively, the results demonstrate that W-tagging represents a powerful way to increase selective peptide internalization in melanoma cells, resulting in toxicity against these, but not against the non-malignant cells. These effects were shown to be due to increased peptide adsorption to the outer membrane in melanoma cells, caused by the presence of anionic lipids such as phosphatidylserine and ganglioside GM1, and to peptide effects on mitochondria membranes and resulting apoptosis. In addition, the possibility of using W-tagged peptides for targeted uptake of nanoparticles/drug carriers in melanoma was demonstrated, as was the possibility to open up the outer membrane of melanoma cells in order to facilitate uptake of low Mw anticancer drugs, here demonstrated for doxorubicin.

Place, publisher, year, edition, pages
2016. Vol. 6, 24952
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-297351DOI: 10.1038/srep24952ISI: 000374906000001PubMedID: 27117225OAI: oai:DiVA.org:uu-297351DiVA: diva2:941952
Funder
Swedish Research Council, 2012-1842Swedish Research Council, 2012-1883Knut and Alice Wallenberg Foundation
Available from: 2016-06-23 Created: 2016-06-22 Last updated: 2017-11-28Bibliographically approved

Open Access in DiVA

fulltext(1961 kB)47 downloads
File information
File name FULLTEXT01.pdfFile size 1961 kBChecksum SHA-512
e017d23ea28c277d45be2242285ba592c2650d7f884bfb3fd2de7f8f0e8dbb5986b3b8257048157b3d18e63b858fdff6dc77c5f9fa22800eb6f70a32006dbccd
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Singh, ShaliniMalmsten, Martin

Search in DiVA

By author/editor
Singh, ShaliniMalmsten, Martin
By organisation
Department of Pharmacy
In the same journal
Scientific Reports
Pharmaceutical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 47 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 203 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf