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A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor
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2016 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 7, 11222Article in journal (Refereed) PublishedText
Abstract [en]

Deciphering the mechanisms directing transcription factors (TFs) to specific genome regions is essential to understand and predict transcriptional regulation. TFs recognize short DNA motifs primarily through their DNA-binding domain. Some TFs also possess an oligomerization domain suspected to potentiate DNA binding but for which the genome-wide influence remains poorly understood. Here we focus on the LEAFY transcription factor, a master regulator of flower development in angiosperms. We have determined the crystal structure of its conserved amino-terminal domain, revealing an unanticipated Sterile Alpha Motif oligomerization domain. We show that this domain is essential to LEAFY floral function. Moreover, combined biochemical and genome-wide assays suggest that oligomerization is required for LEAFY to access regions with low-affinity binding sites or closed chromatin. This finding shows that domains that do not directly contact DNA can nevertheless have a profound impact on the DNA binding landscape of a TF.

Place, publisher, year, edition, pages
2016. Vol. 7, 11222
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Cell Biology
URN: urn:nbn:se:umu:diva-121484DOI: 10.1038/ncomms11222ISI: 000374573500001PubMedID: 27097556OAI: diva2:938913
Available from: 2016-06-17 Created: 2016-06-02 Last updated: 2016-06-17Bibliographically approved

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Schmid, Markus
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Umeå Plant Science Centre (UPSC)Department of Plant Physiology
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