Identification of the tick-borne pathogens Anaplasma phagocytophilum, Neoehrlichia mikurensis and Rickettsia in Swedish ticks: Investigation of transovarial transmission and co-infection
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Globally, vector borne diseases cause more than a million deaths each year and more than a billion infections in humans. Ticks are of big medicinal importance since they can transmit pathogens that can cause serious infections. Some recently discovered pathogens that can cause infections in humans are Anaplasma phagocytophilum (A. phagocytophilum) that can cause human granulocytic anaplasmosis (HGA) and Candidatus Neoehrlichia mikurensis (N. mikurensis) that can cause Neoehrlichiosis. It is still widely unknown how prevalent these pathogens are, if ticks can be infected with both of these pathogens and if these pathogens can be transovarially transmitted from adult female to egg and larvae. This study aims to screen for these pathogens in collected ticks from southern Sweden and to detect eventual co-infections and transovarial transmission. A real-time qPCR assay targeting the 16S rRNA gene of N. mikurensis and other Anaplasmataceae was applied on 1356 Ixodes ricinus (I. ricinus) ticks collected from 5 sites in southern Sweden. Positive samples were subjected to Sanger sequencing. A. phagocytophilum occurred in 4.64 % of the ticks, N. mikurensis occurred in 1.33 % of the ticks and also Rickettsia was found to occur in 6.27 % of the ticks. No co-infection was detected. Some samples of tick larvae showed positive results after qPCR, indicating transovarial transmission, but none of the sequences were readable.
Place, publisher, year, edition, pages
2016. , 23 p.
Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis, co-infections, Ixodes ricinus, real-time qPCR, Rickettsia, Sanger sequencing, transovarial transmission.
IdentifiersURN: urn:nbn:se:lnu:diva-53366ISRN: 2016:BL5OAI: oai:DiVA.org:lnu-53366DiVA: diva2:935598
Subject / course
Biomedical Laboratory Science
Biomedical Laboratory Science Programme, 180 credits
2016-05-30, Sal N144, Linnéuniversitetet i Kalmar, 09:00 (Swedish)
Waldenström, Jonas, Professor
Edman, Kjell, Universitetslektor