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Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding
Univ Oxford, ORCRB, Struct Genom Consortium, Roosevelt Dr, Oxford OX3 7DQ, England..
Univ Oxford, ORCRB, Struct Genom Consortium, Roosevelt Dr, Oxford OX3 7DQ, England.;Univ Huddersfield, Sch Appl Sci, Dept Biol Sci, Huddersfield HD1 3DH, W Yorkshire, England..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology. Univ Oxford, ORCRB, Struct Genom Consortium, Roosevelt Dr, Oxford OX3 7DQ, England..
Univ Oxford, ORCRB, Struct Genom Consortium, Roosevelt Dr, Oxford OX3 7DQ, England..
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2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 2, e0148762Article in journal (Refereed) Published
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Abstract [en]

The MAGE (melanoma associated antigen) protein family are tumour-associated proteins normally present only in reproductive tissues such as germ cells of the testis. The human genome encodes over 60 MAGE genes of which one class (containing MAGE-A3 and MAGE-A4) are exclusively expressed in tumours, making them an attractive target for the development of targeted and immunotherapeutic cancer treatments. Some MAGE proteins are thought to play an active role in driving cancer, modulating the activity of E3 ubiquitin ligases on targets related to apoptosis. Here we determined the crystal structures of MAGE-A3 and MAGE-A4. Both proteins crystallized with a terminal peptide bound in a deep cleft between two tandem-arranged winged helix domains. MAGE-A3 (but not MAGE-A4), is predominantly dimeric in solution. Comparison of MAGE-A3 and MAGE-A3 with a structure of an effector-bound MAGE-G1 suggests that a major conformational rearrangement is required for binding, and that this conformational plasticitymay be targeted by allosteric binders.

Place, publisher, year, edition, pages
2016. Vol. 11, no 2, e0148762
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Cancer and Oncology
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URN: urn:nbn:se:uu:diva-282488DOI: 10.1371/journal.pone.0148762ISI: 000371164700015PubMedID: 26910052OAI: oai:DiVA.org:uu-282488DiVA: diva2:917109
Funder
GlaxoSmithKline (GSK)Wellcome trust, 092809/Z/10/ZNIH (National Institute of Health), 1 R21 CA191898-01
Available from: 2016-04-05 Created: 2016-04-05 Last updated: 2017-11-30Bibliographically approved

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