Analysis of RNA and DNA sequencing data: Improved bioinformatics applications
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Massively parallel sequencing has rapidly revolutionized DNA and RNA research. Sample preparations are steadfastly advancing, sequencing costs have plummeted and throughput is ever growing. This progress has resulted in exponential growth in data generation with a corresponding demand for bioinformatic solutions. This thesis addresses methodological aspects of this sequencing revolution and applies it to selected biological topics.
Papers I and II are technical in nature and concern sample preparation and data anal- ysis of RNA sequencing data. Paper I is focused on RNA degradation and paper II on generating strand specific RNA-seq libraries.
Paper III and IV deal with current biological issues. In paper III, whole exomes of cancer patients undergoing chemotherapy are sequenced and their genetic variants associ- ated to their toxicity induced adverse drug reactions. In paper IV a comprehensive view of the gene expression of the endometrium is assessed from two time points of the menstrual cycle.
Together these papers show relevant aspects of contemporary sequencing technologies and how it can be applied to diverse biological topics.
Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2016. , 135 p.
TRITA-BIO-Report, ISSN 1654-2312 ; 2016:2
RNA sequencing, exome sequencing, bioinformatics, gene expression, differential expression, variant calling
Bioinformatics and Systems Biology
Research subject Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-184158ISBN: 978-91-7595-894-1OAI: oai:DiVA.org:kth-184158DiVA: diva2:915153
2016-04-22, Inghesalen, Tomtebodavägen 18A, Solna, Stockholm, 10:00 (English)
Anders, Simon, Dr.
Lundeberg, Joakim, Professor
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation
QC 201603292016-03-292016-03-292016-03-29Bibliographically approved
List of papers