Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Dose‑dependent autophagic effectof titanium dioxide nanoparticles in humanHaCaT cells at non‑cytotoxic levels
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. (Cristobal Lab, environmental proteomics)
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Luxembourg Institute of Science and Technology, Luxembourg.
Stockholm University, Sweden. (Environmental Proteomics)
Show others and affiliations
2016 (English)In: Journal of Nanobiotechnology, ISSN 1477-3155, E-ISSN 1477-3155, Vol. 14, no 22, 1-13 p.Article in journal (Refereed) Published
Abstract [en]

Background: Interactions between nanoparticles and cells are now the focus of a fast-growing area of research.Though many nanoparticles interact with cells without any acute toxic responses, metal oxide nanoparticles includingthose composed of titanium dioxide (TiO2-NPs) may disrupt the intracellular process of macroautophagy.Autophagy plays a key role in human health and disease, particularly in cancer and neurodegenerative diseases. Weherein investigated the in vitro biological effects of TiO2-NPs (18 nm) on autophagy in human keratinocytes (HaCaT)cells at non-cytotoxic levels.Results: TiO2-NPs were characterized by transmission electron microscopy (TEM) and dynamic light scatteringtechniques. Cellular uptake, as evaluated by TEM and NanoSIMS revealed that NPs internalization led to the formationof autophagosomes. TiO2-NPs treatment did not reduce cell viability of HaCaT cells nor increased oxidative stress. Cellularautophagy was additionally evaluated by confocal microscopy using eGFP-LC3 keratinocytes, western blottingof autophagy marker LC3I/II, immunodetection of p62 and NBR1 proteins, and gene expression of LC3II, p62, NBR1,beclin1 and ATG5 by RT-qPCR. We also confirmed the formation and accumulation of autophagosomes in NPs treatedcells with LC3-II upregulation. Based on the lack of degradation of p62 and NBR1 proteins, autophagosomes accumulationat a high dose (25.0 μg/ml) is due to blockage while a low dose (0.16 μg/ml) promoted autophagy. Cellularviability was not affected in either case.Conclusions: The uptake of TiO2-NPs led to a dose-dependent increase in autophagic effect under non-cytotoxicconditions. Our results suggest dose-dependent autophagic effect over time as a cellular response to TiO2-NPs. Mostimportantly, these findings suggest that simple toxicity data are not enough to understand the full impact of TiO2-NPsand their effects on cellular pathways or function.

Place, publisher, year, edition, pages
BioMed Central, 2016. Vol. 14, no 22, 1-13 p.
Keyword [en]
Autophagy, Cell-nanoparticle interactions, Dose, Keratinocytes, Titanium dioxide nanoparticles
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:liu:diva-126342DOI: 10.1186/s12951-016-0174-0ISI: 000372577700001PubMedID: 27001369OAI: oai:DiVA.org:liu-126342DiVA: diva2:913705
Projects
Nanoimpact
Funder
Swedish Research Council, 621-2011-5267
Note

Funding agencies: Swedish Research Council-Natural Science; VR-NT; Carl Trygger Foundation; Oscar and Lilli Lamms Minne Foundation; Angpanneforening Research foundation; IKERBASQUE Basque Foundation for science; VINNOVA - County Councils of Ostergotland, Sweden; Linkoping 

Available from: 2016-03-22 Created: 2016-03-22 Last updated: 2017-11-30Bibliographically approved

Open Access in DiVA

fulltext(2857 kB)77 downloads
File information
File name FULLTEXT01.pdfFile size 2857 kBChecksum SHA-512
99afd22d88791b49c13239fe1260483e0ae7e786d1ad43bc72adf8c12dab5fed5f0b1cb5317c28a307bd94ffd1516378f519fb6df2873d012a099d49583cb333
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Lopes, Viviana RLoitto, VesaCristobal, Susana
By organisation
Division of Cell BiologyFaculty of Medicine and Health SciencesDivision of Microbiology and Molecular Medicine
In the same journal
Journal of Nanobiotechnology
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 77 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 352 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf