Change search
ReferencesLink to record
Permanent link

Direct link
Metabolic potential of a single cell belonging to one of the most abundant lineages in freshwater bacterioplankton
Jena School for Microbial Communication (JSMC) and Microbial Ecology Group at Friedrich Schiller University Jena, Jena, Germany.
Show others and affiliations
2013 (English)In: The ISME Journal, ISSN 1751-7362, E-ISSN 1751-7370, Vol. 7, no 1, 137-147 p.Article in journal (Refereed) PublishedText
Abstract [en]

Actinobacteria within the acI lineage are often numerically dominating in freshwater ecosystems, where they can account for >50% of total bacteria in the surface water. However, they remain uncultured to date. We thus set out to use single-cell genomics to gain insights into their genetic make-up, with the aim of learning about their physiology and ecological niche. A representative from the highly abundant acI-B1 group was selected for shotgun genomic sequencing. We obtained a draft genomic sequence in 75 larger contigs (sum=1.16 Mb), with an unusually low genomic G+C mol% (~42%). Actinobacteria core gene analysis suggests an almost complete genome recovery. We found that the acI-B1 cell had a small genome, with a rather low percentage of genes having no predicted functions (~15%) as compared with other cultured and genome-sequenced microbial species. Our metabolic reconstruction hints at a facultative aerobe microorganism with many transporters and enzymes for pentoses utilization (for example, xylose). We also found an actinorhodopsin gene that may contribute to energy conservation under unfavorable conditions. This project reveals the metabolic potential of a member of the global abundant freshwater Actinobacteria.

Place, publisher, year, edition, pages
2013. Vol. 7, no 1, 137-147 p.
National Category
URN: urn:nbn:se:uu:diva-279793DOI: 10.1038/ismej.2012.86OAI: diva2:908956
Available from: 2016-03-03 Created: 2016-03-03 Last updated: 2016-03-04Bibliographically approved

Open Access in DiVA

fulltext(592 kB)15 downloads
File information
File name FULLTEXT02.pdfFile size 592 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Garcia, Sarahi L
In the same journal
The ISME Journal

Search outside of DiVA

GoogleGoogle Scholar
Total: 15 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 18 hits
ReferencesLink to record
Permanent link

Direct link