Oxygen-dependent regulation of key components in microbial chlorate respiration
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Contamination of perchlorate and chlorate in nature is primarily the result of various industrial processes. The microbial respiration of these oxyanions of chlorine plays a major role in reducing the society’s impact on the environment. The focus with this thesis is to investigate the oxygen-dependent regulation of key components involved in the chlorate respiration in the gram‑negative bacterium Ideonella dechloratans. Chlorate metabolism is based on the action of the enzymes chlorate reductase and chlorite dismutase and results in the end products molecular oxygen and chloride ion. Up‑regulation of chlorite dismutase activity in the absence of oxygen is demonstrated to occur at the transcriptional level, with the participation of the transcriptional fumarate and nitrate reduction regulator (FNR). Also, the chlorate reductase enzyme was shown to be regulated at the transcriptional level with the possible involvement of additional regulating mechanisms as well. Interestingly, the corresponding chlorate reductase operon was found to be part of a polycistronic mRNA which also comprises the gene for a cytochrome c and a putative transcriptional regulator protein.
Place, publisher, year, edition, pages
Karlstad: Karlstads universitet, 2016.
Karlstad University Studies, ISSN 1403-8099 ; 13
Anaerobic respiration, Gene expression, Chlorate, Chlorate reductase, Chlorite dismutase
Research subject Chemistry
IdentifiersURN: urn:nbn:se:kau:diva-40698ISBN: 978-91-7063-692-9OAI: oai:DiVA.org:kau-40698DiVA: diva2:906974
2016-04-08, Rejmersalen, 9C204, Karlstad, 09:00 (Swedish)
Nordlund, Stefan, Professor
Nilsson, Thomas, Professor
List of papers