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How to Properly Measure a Current-Voltage Relation?: Interpolation vs. Ramp Methods Applied to Studies of GABA(A) Receptors
Umeå University, Sweden.
Umeå University, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Umeå University, Sweden.
Umeå University, Sweden.
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2016 (English)In: Frontiers in Cellular Neuroscience, ISSN 1662-5102, E-ISSN 1662-5102, Vol. 10, no 10Article in journal (Refereed) PublishedText
Abstract [en]

The relation between current and voltage, I-V relation, is central to functional analysis of membrane ion channels. A commonly used method, since the introduction of the voltage-clamp technique, to establish the I-V relation depends on the interpolation of current amplitudes recorded at different steady voltages. By a theoretical computational approach as well as by experimental recordings from GABA(A) receptor mediated currents in mammalian central neurons, we here show that this interpolation method may give reversal potentials and conductances that do not reflect the properties of the channels studied under conditions when ion flux may give rise to concentration changes. Therefore, changes in ion concentrations may remain undetected and conclusions on changes in conductance, such as during desensitization, may be mistaken. In contrast, an alternative experimental approach, using rapid voltage ramps, enable I-V relations that much better reflect the properties of the studied ion channels.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2016. Vol. 10, no 10
Keyword [en]
current-voltage relation; voltage clamp; reversal potential; conductance; concentration changes; interpolation; voltage ramp; ion channel
National Category
Clinical Medicine
URN: urn:nbn:se:liu:diva-125294DOI: 10.3389/fncel.2016.00010ISI: 000369141200001PubMedID: 26869882OAI: diva2:906420

Funding Agencies|Swedish Research Council [22292]; Gunvor och Josef Aners Stiftelse

Available from: 2016-02-24 Created: 2016-02-19 Last updated: 2016-03-16

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