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Simultaneous Multiplexed Measurement of RNA and Proteins in Single Cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Neuro-Oncology.
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2016 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 14, no 2, 380-389 p.Article in journal (Refereed) Published
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Abstract [en]

Significant advances have been made in methods to analyze genomes and transcriptomes of single cells, but to fully define cell states, proteins must also be accessed as central actors defining a cell's phenotype. Methods currently used to analyze endogenous protein expression in single cells are limited in specificity, throughput, or multiplex capability. Here, we present an approach to simultaneously and specifically interrogate large sets of protein and RNA targets in lysates from individual cells, enabling investigations of cell functions and responses. We applied our method to investigate the effects of BMP4, an experimental therapeutic agent, on early-passage glioblastoma cell cultures. We uncovered significant heterogeneity in responses to treatment at levels of RNA and protein, with a subset of cells reacting in a distinct manner to BMP4. Moreover, we found overall poor correlation between protein and RNA at the level of single cells, with proteins more accurately defining responses to treatment.

Place, publisher, year, edition, pages
2016. Vol. 14, no 2, 380-389 p.
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Cell and Molecular Biology
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URN: urn:nbn:se:uu:diva-276810DOI: 10.1016/j.celrep.2015.12.021ISI: 000368101600020PubMedID: 26748716OAI: oai:DiVA.org:uu-276810DiVA: diva2:903544
Funder
EU, FP7, Seventh Framework Programme, 259796EU, FP7, Seventh Framework Programme, 294409Swedish Research CouncilKnut and Alice Wallenberg FoundationSwedish Cancer Society
Available from: 2016-02-16 Created: 2016-02-16 Last updated: 2017-11-30Bibliographically approved

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