Comparative genomics and genome biology of invasive Campylobacter jejuni
2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, 17300Article in journal (Refereed) PublishedText
Campylobacter jejuni is a major pathogen in bacterial gastroenteritis worldwide and can cause bacteremia in severe cases. C. jejuni is highly structured into clonal lineages of which the ST677CC lineage has been overrepresented among C. jejuni isolates derived from blood. In this study, we characterized the genomes of 31 C. jejuni blood isolates and 24 faecal isolates belonging to ST677CC in order to study the genome biology related to C. jejuni invasiveness. We combined the genome analyses with phenotypical evidence on serum resistance which was associated with phase variation of wcbK; a GDP-mannose 4,6-dehydratase involved in capsular biosynthesis. We also describe the finding of a Type III restriction-modification system unique to the ST-794 sublineage. However, features previously considered to be related to pathogenesis of C. jejuni were either absent or disrupted among our strains. Our results refine the role of capsule features associated with invasive disease and accentuate the possibility of methylation and restriction enzymes in the potential of C. jejuni to establish invasive infections. Our findings underline the importance of studying clinically relevant well-characterized bacterial strains in order to understand pathogenesis mechanisms important in human infections.
Place, publisher, year, edition, pages
2015. Vol. 5, 17300
IdentifiersURN: urn:nbn:se:uu:diva-270445DOI: 10.1038/srep17300ISI: 000365301500001PubMedID: 26603914OAI: oai:DiVA.org:uu-270445DiVA: diva2:898365
FunderSwedish Research Council, 521-2011-3527Swedish Research Council Formas, 221-2012-1442