High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility
2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, no 18538Article in journal (Refereed) PublishedText
The study attempted to clarify the role of total antioxidant capacity of seminal plasma (SP-TAC) on boar sperm survival and fertility after artificial insemination (AI). SP-TAC differed (P < 0.001) among boars (no = 15) and, to a lesser degree, among ejaculates within male (4 ejaculates/boar). SP-TAC also differed (P < 0.001) among ejaculate fractions (43 ejaculates and 3 fractions per ejaculate), of which the sperm-peak portion of the sperm rich ejaculate fraction (SRF) had the highest SP-TAC. SP-TAC was not correlated with sperm quality (motility and viability) or functionality (intracellular ROS generation and lipid peroxidation) of liquid AI-semen samples stored at 17 degrees C for 72 h (90 AI-samples), but the decline in sperm quality was larger (P < 0.05) in ejaculates with low, compared with high SP-TAC (hierarchically grouped). The SP-TAC differences among ejaculate portions agree with sperm cryosurvival rates (14 ejaculates from 7 boars), showing sperm from sperm-peak portion better (P < 0.01) post-thaw quality and functionality than those from the entire ejaculate (mainly post-SRF). Boars (no = 18) with high SP-TAC (hierarchically grouped) had higher (P < 0.05) fertility outcomes (5,546 AI-sows) than those with low SP-TAC. Measurement of SP-TAC ought to be a discriminative tool to prognosis fertility in breeding boars.
Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2015. Vol. 5, no 18538
IdentifiersURN: urn:nbn:se:liu:diva-124123DOI: 10.1038/srep18538ISI: 000367080500001PubMedID: 26688188OAI: oai:DiVA.org:liu-124123DiVA: diva2:896856
Funding Agencies|MINECO Madrid (Spain) [AGL2012-39903]; FEDER funds (EU); FORMAS, Stockholm [221-2011-512]; Swedish Research Council VR, Stockholm [521-2011-6553]; MECD (Madrid, Spain); Seneca Foundation (Murcia, Spain)2016-01-222016-01-192016-02-11