The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes
2015 (English)In: EBioMedicine, ISSN 0360-0637, E-ISSN 2352-3964, Vol. 2, no 10, 1351-1363 p.Article in journal (Refereed) PublishedText
Glioblastoma (GBM) is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called gliomastem cells (GSCs). To meet the present shortage of relevant GBM cell (GC) lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC) resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional sub-types. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research.
Place, publisher, year, edition, pages
2015. Vol. 2, no 10, 1351-1363 p.
Glioblastoma, Cell culture, Stem cell culture condition, Molecular subtype, Xenograft models
Cancer and Oncology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:uu:diva-274354DOI: 10.1016/j.ebiom.2015.08.026ISI: 000365959700034PubMedID: 26629530OAI: oai:DiVA.org:uu-274354DiVA: diva2:896332
De två sista författarna delar sistaförfattarskapet.2016-01-212016-01-212016-04-04Bibliographically approved