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Method Development for Quantification of Different Persistent Organic Pollutants in Ringed Seal (Phoca hispida) from the Baltic Sea
Örebro University, School of Science and Technology, Örebro University, Sweden.
2016 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Persistent organic pollutants such as polychlorinated biphenyls (PCBs), DDT and polybrominated diphenyl ethers (PBDEs) tend to accumulate in biota and are transferred through the aquatic food web, which result in a high accumulation in marine mammals. In recent years various novel flame retardants (nBFRs), which have replaced the banned PBDEs, have also started to occur in the environment. These nBFRs have similar properties as PBDEs, such as long-range transport and accumulation in biota. The purpose with this study was to evaluate a method by using pre-packed silica columns for quantification of PCBs, DDT, PBDEs and nBFRs in seal blubber, in order to facilitate the pre-treatment and decrease the time. To elute the different POPs from the pre-packed silica column; hexane, toluene and dichloromethane were used in different stages. By using this method levels of PCB and DDT were determined. For DDT the concentration was 8.28 ng/g lipid and 8.94 ng/g lipid for the two samples that was analysed, and the analysis of the PCBs showed a higher trend for the higher chlorinated PCBs. As the pre-packed silica columns are a relative new method. Further studies are therefore needed on these columns to further improve the sample clean-up and fractionation of the different POPs in environmental samples.

Place, publisher, year, edition, pages
Keyword [en]
Ringed seal, Baltic Sea, Persistent Organic Pollutants, Sample pre-treatment
National Category
Chemical Sciences
URN: urn:nbn:se:oru:diva-47426OAI: diva2:894661
Subject / course
Available from: 2016-01-15 Created: 2016-01-15 Last updated: 2016-01-15Bibliographically approved

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School of Science and Technology, Örebro University, Sweden
Chemical Sciences

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