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SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy
Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn ISIC, Inst Bioengn, NCCR Chem Biol, CH-1015 Lausanne, Switzerland..
Austrian Acad Sci IMBA, Inst Mol Biotechnol, A-1030 Vienna, Austria..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn ISIC, Inst Bioengn, NCCR Chem Biol, CH-1015 Lausanne, Switzerland..
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2015 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 6, 8497Article in journal (Refereed) Published
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Abstract [en]

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

Place, publisher, year, edition, pages
2015. Vol. 6, 8497
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-269271DOI: 10.1038/ncomms9497ISI: 000364928600001PubMedID: 26423723OAI: oai:DiVA.org:uu-269271DiVA: diva2:882683
Funder
EU, European Research Council, 281198EU, FP7, Seventh Framework Programme, 241548EU, FP7, Seventh Framework Programme, 258068
Available from: 2015-12-15 Created: 2015-12-15 Last updated: 2017-12-01Bibliographically approved

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