Change search
ReferencesLink to record
Permanent link

Direct link
Stem Cell Therapy in Injured Vocal Folds: A Three-Month Xenograft Analysis of Human Embryonic Stem Cells
Umea Univ, Unit Clin Res Ctr, Dept Publ Hlth & Clin Med, S-90187 Umea, Sweden.;Dept Otorhinolaryngol, S-83183 Ostersund, Sweden..
Karolinska Inst, Ctr Hematol & Regenerat Med, Dept Lab Med, S-14186 Stockholm, Sweden..
Umea Univ, Ctr Mol Med, S-90187 Umea, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Show others and affiliations
2015 (English)In: BioMed Research International, ISSN 2314-6133, E-ISSN 2314-6141, 754876Article in journal (Refereed) PublishedText
Abstract [en]

We have previously shown that human embryonic stem cell (hESC) therapy to injured rabbit vocal folds (VFs) induces human tissue generation with regained VF vibratory capacity. The aims of this study were to test the sustainability of such effect and to what extent derivatives of the transplanted hESCs are propagated in the VFs. The VFs of 14 New Zealand rabbits were injured by a localized resection. HESCs were transplanted to 22 VFs which were analyzed for persistence of hESCs after six weeks and after three months. At three months, the VFs were also analyzed for viscoelasticity, measured as dynamic viscosity and elastic modulus, for the lamina propria (Lp) thickness and relative content of collagen type I. Three months after hESC cell therapy, the dynamic viscosity and elastic modulus of the hESC treated VFs were similar to normal controls and lower than untreated VFs (P <= 0.011). A normalized VF architecture, reduction in collagen type I, and Lp thickness were found compared with untreated VFs (P <= 0.031). At three months, no derivatives of hESCs were detected. HESCs transplanted to injured rabbit VFs restored the vibratory characteristics of the VFs, with maintained restored function for three months without remaining hESCs or derivatives.

Place, publisher, year, edition, pages
2015. 754876
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
URN: urn:nbn:se:uu:diva-268724DOI: 10.1155/2015/754876ISI: 000364082500001OAI: diva2:878773
The Karolinska Institutet's Research Foundation
Available from: 2015-12-09 Created: 2015-12-09 Last updated: 2015-12-09Bibliographically approved

Open Access in DiVA

fulltext(2671 kB)55 downloads
File information
File name FULLTEXT01.pdfFile size 2671 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Cedervall, Jessica
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
BioMed Research International
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 55 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 177 hits
ReferencesLink to record
Permanent link

Direct link