Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Distribution of circular proteins in plants: large-scale mapping of cyclotides in the Violaceae
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy. Univ Addis Ababa, Sch Pharm, Dept Pharmacognosy, Addis Ababa, Ethiopia..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Univ Queensland, Inst Mol Biosci, Chem & Struct Biol Div, Craik Lab, Brisbane, Qld, Australia..
Show others and affiliations
2015 (English)In: Frontiers in Plant Science, ISSN 1664-462X, E-ISSN 1664-462X, Vol. 6, 855Article in journal (Refereed) Published
Abstract [en]

During the last decade there has been increasing interest in small circular proteins found in plants of the violet family (Violaceae). These so-called cyclotides consist of a circular chain of approximately 30 amino acids, including six cysteines forming three disulfide bonds, arranged in a cyclic cystine knot (CCK) motif. In this study we map the occurrence and distribution of cyclotides throughout the Violaceae. Plant material was obtained from herbarium sheets containing samples up to 200 years of age. Even the oldest specimens contained cyclotides in the preserved leaves, with no degradation products observable, confirming their place as one of the most stable proteins in nature. Over 200 samples covering 17 of the 23-31 genera in Violaceae were analyzed, and cyclotides were positively identified in 150 species. Each species contained a unique set of between one and 25 cyclotides, with many exclusive to individual plant species. We estimate the number of different cyclotides in the Violaceae to be 5000-25,000, and propose that cyclotides are ubiquitous among all Violaceae species. Twelve new cyclotides from six phylogenetically dispersed genera were sequenced. Furthermore, the first glycosylated derivatives of cyclotides were identified and characterized, further increasing the diversity and complexity of this unique protein family.

Place, publisher, year, edition, pages
2015. Vol. 6, 855
Keyword [en]
cyclotide, Violaceae, plant protein, plant peptide, stable proteins
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-268431DOI: 10.3389/fpls.2015.00855ISI: 000364373400001OAI: oai:DiVA.org:uu-268431DiVA: diva2:876716
Funder
Swedish Research Council, 2012-5063Swedish Research Council Formas
Available from: 2015-12-04 Created: 2015-12-04 Last updated: 2017-12-01Bibliographically approved

Open Access in DiVA

fulltext(4060 kB)178 downloads
File information
File name FULLTEXT01.pdfFile size 4060 kBChecksum SHA-512
5ea9b1d7f2311b3d36217990bbb07e8b60543e221c8ce9e1aa54d6f28b90458b7af93e2e7797e200d998c11cd6f194885fa1ded2975b8639161c5150aa884263
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Burman, RobertGöransson, Ulf
By organisation
Division of Pharmacognosy
In the same journal
Frontiers in Plant Science
Pharmacology and Toxicology

Search outside of DiVA

GoogleGoogle Scholar
Total: 178 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 473 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf