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Single-molecule imaging with longer X-ray laser pulses
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Molecular and condensed matter physics.
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Molecular and condensed matter physics. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.ORCID iD: 0000-0001-7328-0400
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2015 (English)In: IUCrJ, ISSN 0972-6918, E-ISSN 2052-2525, Vol. 2, 661-674 p.Article in journal (Refereed) Published
Abstract [en]

During the last five years, serial femtosecond crystallography using X-ray laser pulses has been developed into a powerful technique for determining the atomic structures of protein molecules from micrometre- and sub-micrometre-sized crystals. One of the key reasons for this success is the ‘self-gating’ pulse effect, whereby the X-ray laser pulses do not need to outrun all radiation damage processes. Instead, X-ray-induced damage terminates the Bragg diffraction prior to the pulse completing its passage through the sample, as if the Bragg diffraction were generated by a shorter pulse of equal intensity. As a result, serial femtosecond crystallography does not need to be performed with pulses as short as 5–10fs, but can succeed for pulses 50–100fs in duration. It is shown here that a similar gating effect applies to single-molecule diffraction with respect to spatially uncorrelated damage processes like ionization and ion diffusion. The effect is clearly seen in calculations of the diffraction contrast, by calculating the diffraction of the average structure separately to the diffraction from statistical fluctuations of the structure due to damage (‘damage noise’). The results suggest that sub-nanometre single-molecule imaging with 30–50fs pulses, like those produced at currently operating facilities, should not yet be ruled out. The theory presented opens up new experimental avenues to measure the impact of damage on single-particle diffraction, which is needed to test damage models and to identify optimal imaging conditions.

Place, publisher, year, edition, pages
2015. Vol. 2, 661-674 p.
Keyword [en]
coherent diffractive imaging, single-molecule imaging, radiation damage, ‘self-gated’ pulses, XFELs
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:uu:diva-267778DOI: 10.1107/S2052252515016887ISI: 000364415900011OAI: oai:DiVA.org:uu-267778DiVA: diva2:874256
Funder
Swedish Foundation for Strategic Research Swedish Research Council
Available from: 2015-11-26 Created: 2015-11-26 Last updated: 2017-12-01Bibliographically approved

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