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A Possible Industrial Solution to Ferment Lignocellulosic Hydrolyzate to Ethanol: Continuous Cultivation with Flocculating Yeast
University of Borås, School of Engineering.
University of Borås, School of Engineering.ORCID iD: 0000-0003-4887-2433
2007 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 8, no 9, 920-932 p.Article in journal (Refereed)
Abstract [en]

Cultivation of toxic lignocellulosic hydrolyzates has been a research topic in recent decades. Although several methods have been proposed, there has been doubt about their industrial applications. The current work deals with a solution to this problem which has a good potential application in industrial scale. A toxic dilute-acid hydrolyzate was continuously cultivated using a high-cell-density flocculating yeast in a single and serial bioreactor which was equipped with a settler to recycle the cells back to the bioreactors. No prior detoxification was necessary to cultivate the hydrolyzates, as the flocks were able to detoxify it in situ. The experiments were successfully carried out at dilution rates up to 0.52 h-1. The cell concentration inside the bioreactors was between 23 and 35 g-DW/L, while this concentration in the effluent of the settlers was 0.320.05 g-DW/L. The ethanol yield of 0.42-0.46 g/g-consumed sugar was achieved, and the residual sugar concentration was less than 6% of the initial fermentable sugar (glucose, galactose and mannose) of 35.2 g/L.

Place, publisher, year, edition, pages
Molecular Diversity Preservation International (MDPI) AG. , 2007. Vol. 8, no 9, 920-932 p.
Keyword [en]
dilute-acid lignocellulosic hydrolysate, scharomyces cerevisiae, flocculting yeast, ethanol, HMF, furfural, continuous cultivation
Keyword [sv]
Energi och material
National Category
Industrial Biotechnology
URN: urn:nbn:se:hb:diva-2294DOI: 10.3390/i8090920Local ID: 2320/2957OAI: diva2:870384
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2016-03-03

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Purwadi, R.Taherzadeh, M.J.
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