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A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin
University of Borås, School of Engineering. (Biotechnology)
University of Borås, School of Engineering. (Biotechnology)
University of Borås, School of Engineering. (Biotechnology)ORCID iD: 0000-0003-4887-2433
2014 (English)In: Journal of Bioprocessing & Biotechniques, ISSN 2155-9821, Vol. 4, 181- p.Article in journal (Refereed)
Sustainable development
The content falls within the scope of Sustainable Development
Abstract [en]

The ability to hydrolyze keratin, a rigid and strongly cross-linked protein in the waste of poultry feather and sheep wool, has made keratinase production by microorganisms highly important to the biotechnological industry. A proteindegrading bacterium (C4) was isolated from compost. Based on morphology and biochemical tests, along with 16S rRNA sequencing, the isolated C4 was tentatively identified as Bacillus sp. C4 (2008). The proteolytic activity of the Bacillus sp. C4 strain was broadly specific; it degraded keratinous and non-keratinous proteins to different degrees. Pea pods as substrate generated the highest protease production, followed by soybean meal and sheep wool. Notwithstanding, using wool keratin as a sole source of carbon and nitrogen yielded the highest level of soluble proteins. Furthermore, the C4 bacterium grew well, and produced a significant level of keratinase when using wool and feather as substrates. Supplementing the medium with yeast extract and peptone shortened the time required for feather degradation, but delayed the onset of the wool keratin hydrolysis with two days. The predominant amino acids released in feather hydrolysate were tyrosine, phenylalanine, and histidine. In contrast, the wool lysate was rich in aspartic acid, methionine, tyrosine, phenylalanine, histidine, and lysine. Results established that utilizing the C4 strain for keratin degradation in waste management holds considerable potential.

Place, publisher, year, edition, pages
Omics Publishing Group , 2014. Vol. 4, 181- p.
Keyword [en]
Resource Recovery
National Category
Industrial Biotechnology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-1938DOI: 10.4172/2155-9821.1000181Local ID: 2320/14387OAI: oai:DiVA.org:hb-1938DiVA: diva2:870016
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2016-03-03

Open Access in DiVA

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