Change search
ReferencesLink to record
Permanent link

Direct link
Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
University of Borås, School of Engineering. (Biotechnology)
University of Borås, School of Engineering. (Biotechnology)ORCID iD: 0000-0003-4887-2433
2012 (English)In: Electronic Journal of Biotechnology, ISSN 0717-3458, E-ISSN 0717-3458, Vol. 15, no 3, 1-14 p.Article in journal (Refereed) Published
Sustainable development
The content falls within the scope of Sustainable Development
Abstract [en]

Background: Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS8066. Results: The flocculating strain CCUG53310 could rapidly ferment all hexoses in dilute acid spruce hydrolysate, while CBS8066 was strongly inhibited in this medium. In synthetic inhibitory media, CCUG53310 was more tolerant to carboxylic acids and furan aldehydes, but more sensitive than CBS8066 to phenolic compounds. Despite the higher tolerance, the increase in expression of the YAP1, ATR1 and FLR1 genes, known to confer resistance to lignocellulose-derived inhibitors, was generally smaller in CCUG53310 than in CBS8066 in inhibitory media. The flocculation of CCUG53310 was linked to the expression of FLO8, FLO10 and one or more of FLO1, FLO5 or FLO9. Flocculation depended on cell wall proteins and Ca2+ ions, but was almost unaffected by other compounds and pH values typical for lignocellulosic media. Conclusions: S. cerevisiae CCUG53310 can be characterised as being very robust, with great potential for industrial fermentation of lignocellulosic hydrolysates relatively low in phenolic inhibitors.

Place, publisher, year, edition, pages
Pontificia Universidad Catolica de Valparaiso , 2012. Vol. 15, no 3, 1-14 p.
Keyword [en]
biofuel, furfural, lignocellulose, phenolic inhibitors, Saccharomyces cerevisiae, Resource Recovery
National Category
Industrial Biotechnology
Research subject
Resource Recovery
URN: urn:nbn:se:hb:diva-1317DOI: 10.2225/vol15-issue3-fulltext-8ISI: 000307004200008Local ID: 2320/11322OAI: diva2:869341
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2016-03-14

Open Access in DiVA

fulltext(431 kB)7 downloads
File information
File name FULLTEXT01.pdfFile size 431 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Westman, Johan O.Taherzadeh, Mohammad J.
By organisation
School of Engineering
In the same journal
Electronic Journal of Biotechnology
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 7 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 21 hits
ReferencesLink to record
Permanent link

Direct link