Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Embryonic and mature astrocytes exert different effects on neuronal growth in rat ventral mesencephalic slice cultures
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
Show others and affiliations
2015 (English)In: SpringerPlus, E-ISSN 2193-1801, Vol. 4, 558Article in journal (Refereed) Published
Abstract [en]

One obstacle with grafting of dopamine neurons in Parkinson's disease is the insufficient ability of the transplant to reinnervate the host striatum. Another issue is the prospective interaction between the donor fetal tissue and the adult astrocytes of the host. To study nerve fiber growth and its interaction with immature/mature astrocytes, ventral mesencephalic (VM) organotypic rat tissue cultures from embryonic days (E) 12, E14, and E18 were studied up to 35 days in vitro (DIV), and co-cultures of E14 VM tissue and mature green fluorescent protein (GFP)-positive astrocytes were performed. Generally, nerve fibers grew from the tissue slice either in association with a monolayer of migrated astroglia surrounding the tissue (glial-associated), or distal to the astroglia as non-glial-associated outgrowth. The tyrosine hydroxylase (TH)-positive glial-associated nerve fiber outgrowth reached a plateau at 21 DIV in E12 and E14 cultures. In E18 cultures, TH-positive neurons displayed short processes and migrated onto the astrocytes. While the non-glial-associated nerve fiber outgrowth dominated the E14 cultures, it was found absent in E18 cultures. The GFP-positive cells in the VM and GFP-positive astrocyte co-cultures were generally located distal to the monolayer of migrated fetal astrocytes, a few GFP-positive cells were however observed within the astrocytic monolayer. In those cases TH-positive neurons migrated towards the GFP-positive cells. Both the non-glial-and glial-associated nerve fibers grew onto the GFP-positive cells. Taken together, the glial-associated growth has limited outgrowth compared to the non-glial-associated nerve fibers, while none of the outgrowth types were hampered by the mature astrocytes.

Place, publisher, year, edition, pages
2015. Vol. 4, 558
Keyword [en]
Organotypic culture, Ventral mesencephalon, Mature astrocytes, Developmental stages
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-110567DOI: 10.1186/s40064-015-1362-3ISI: 000361917700003PubMedID: 26435904OAI: oai:DiVA.org:umu-110567DiVA: diva2:867843
Available from: 2015-11-06 Created: 2015-10-23 Last updated: 2017-12-01Bibliographically approved

Open Access in DiVA

fulltext(2177 kB)151 downloads
File information
File name FULLTEXT01.pdfFile size 2177 kBChecksum SHA-512
60d48f32a126a94d8630266d7e5d033bb7746ef62e0b9a0ef58a4035a626a4156df29199cb9d0e787b1cc95103854882d876f23858fdb4e8fb79c0153dbfcbec
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hashemian, SanazStrömberg, Ingridaf Bjerkén, Sara
By organisation
Department of Integrative Medical Biology (IMB)
In the same journal
SpringerPlus
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 151 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 177 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf