An in vitro investigation of NECAB2 membrane interactome
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
NECAB2 is a Ca2+-binding protein highly expressed in brain, known to interact with G protein-coupled receptors and altering receptor behavior and downstream signaling. Here we investigate potential NECAB2 interactors in vitro using NECAB2 transfected HEK293 cells and co-immunoprecipitation and mass spectrometry approaches. NECAB2 containing protein complexes were isolated and analyzed using mass spectrometry. MARCKS, known to be involved in dendritic spine formation, was identified as a NECAB2 interacting protein. This interaction might explain the morphological changes and formation of neurite-like structures observed in NECAB2 transfected HEK293 cells. We have established an in vitro model system to study the NECAB2 interactome. However, these findings are still preliminary and additional experiments are needed to validate these results.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:kth:diva-176461OAI: oai:DiVA.org:kth-176461DiVA: diva2:867489
Master of Science - Medical Biotechnology